Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I2 R1 104 44.1 1376607 98.9% 1361464 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,769,479 A→G T136A (ACG→GCG)  ydiK → UPF0118 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,769,4790AG100.0% 140.2 / NA 41T136A (ACG→GCG) ydiKUPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/25);  total (16/25)

TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTG  >  NC_000913/1769339‑1769617
                                                                                                                                             |                                                                                                                                          
tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCg                                                                                                                                          >  2:302891/1‑143 (MQ=255)
      aCGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGa                                                                                                                             >  2:420635/1‑150 (MQ=255)
                 ccGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCtt                                                                                                                 >  1:523178/1‑151 (MQ=255)
                  cGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTta                                                                                                                <  1:490087/151‑1 (MQ=255)
                   gCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTtat                                                                                                               <  1:479009/151‑1 (MQ=255)
                      aaTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                                            <  2:639770/151‑1 (MQ=255)
                              ccATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcactac                                                                                                    <  2:687426/151‑1 (MQ=255)
                                 tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGTGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacca                                                                                                  <  1:111702/150‑1 (MQ=255)
                                      aGCGGTTACCGGACGTCACCCGATCTGGCGGGGCTTAATACCAGTCCGGAGATGGGAGCGCAGCTGTATGCAGGCGGGCACAACTTGCTGGATAT‑GGCGGGGGCGGCGGTCATGCTGGAACTCCCCGCTTATATTCTTcccaccaccacc                                                                                             >  1:155669/1‑150 (MQ=255)
                                        cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTgg                                                                                          <  1:508599/151‑1 (MQ=255)
                                          gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                                        <  2:680675/151‑1 (MQ=255)
                                          gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                                        <  1:174907/151‑1 (MQ=255)
                                                         ccGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAggcggc                                                                         >  2:71086/1‑151 (MQ=255)
                                                            aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGgcgc                                                                       >  2:623727/1‑150 (MQ=255)
                                                            aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGgcgc                                                                       >  1:549801/1‑150 (MQ=255)
                                                              cTGGCGGGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCat                                                                     >  1:656859/1‑150 (MQ=255)
                                                                ggCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCGCCACCTGGTTCGTTGGGCAggcggc                                                                         <  2:495492/144‑1 (MQ=255)
                                                                ggCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCatat                                                                   <  1:480786/150‑1 (MQ=255)
                                                                 gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCg                                                                 >  2:293977/1‑151 (MQ=255)
                                                                    tGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg                                                                <  1:402274/149‑1 (MQ=255)
                                                                     ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                                             <  1:419070/151‑1 (MQ=255)
                                                                     ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                                             >  2:240659/1‑151 (MQ=255)
                                                                     ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                                              <  1:256273/150‑1 (MQ=255)
                                                                          aaTACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGAGCTGCGTTCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTa                                                        <  1:539996/151‑1 (MQ=255)
                                                                            tACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATg                                                      >  1:573491/1‑151 (MQ=255)
                                                                              ccATTACGACGATTGGCGCGAAGCTGTATGAAGGTTGGCACAACTTGCTGGATAT‑GGCGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATGGGCACCACCGCCACCTGGTGCGTGGGACAGGCGGCGCATATCGGgcgtt                                                           <  1:423411/144‑3 (MQ=255)
                                                                                   ccGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCAt                                                <  2:235722/151‑1 (MQ=255)
                                                                                             gcgcGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGAt                                     <  2:677440/151‑1 (MQ=255)
                                                                                               gcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGc                                   >  1:508470/1‑151 (MQ=255)
                                                                                                  aaGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtc                                >  2:317958/1‑151 (MQ=255)
                                                                                                    gCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtctc                              <  2:274173/151‑1 (MQ=255)
                                                                                                        tATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCAACACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTc                           >  2:688185/1‑150 (MQ=255)
                                                                                                          tGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGt                        >  2:294200/1‑151 (MQ=255)
                                                                                                           gCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg                       <  1:279507/151‑1 (MQ=255)
                                                                                                            cAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc                      <  2:519730/151‑1 (MQ=255)
                                                                                                              ggCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGccc                    <  2:487458/151‑1 (MQ=255)
                                                                                                                  ggCACATCTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCCCCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCctgct                <  1:565861/151‑1 (MQ=255)
                                                                                                                      caACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGTTTCGTTGGGCAGTCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTa             <  1:127737/150‑1 (MQ=255)
                                                                                                                           tGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTGTGGTGCATTGTGCGCTGCTGCTTCTCTTCAGTGCCCTGCTGTACTGgc        <  1:574704/150‑1 (MQ=255)
                                                                                                                            gCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGgcg       <  2:625186/150‑1 (MQ=255)
                                                                                                                                gATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTg  >  1:517493/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                          
TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTG  >  NC_000913/1769339‑1769617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A