Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,772,863	C→T	L118L (CTG→TTG)	ydiN →	putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,772,863	0	C	T	100.0%	135.9 / NA	36	L118L (CTG→TTG)	ydiN	putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

GTGATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTA  >  NC_000913/1772719‑1773008

gtgATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATa                                                                                                                                             <  2:158182/151‑1 (MQ=255)

   aTCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACgg                                                                                                                                          >  1:330685/1‑151 (MQ=255)

      tcCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTg                                                                                                                                       >  1:6187/1‑151 (MQ=255)

       ccGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACgg                                                                                                                                          <  2:519128/147‑1 (MQ=255)

          aTAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTa                                                                                                                                   <  2:204314/151‑1 (MQ=255)

             aGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTAccc                                                                                                                                >  2:2874/1‑151 (MQ=255)

                  ggTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCcgcg                                                                                                                            <  2:174245/150‑1 (MQ=255)

                          ggCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGaa                                                                                                                   <  1:568176/151‑1 (MQ=255)

                           gCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAAt                                                                                                                  >  2:191084/1‑151 (MQ=255)

                            cGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATg                                                                                                                 >  1:426936/1‑151 (MQ=255)

                                            aGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCctct                                                                                                 >  1:136943/1‑151 (MQ=255)

                                             gTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTg                                                                                                <  2:301715/151‑1 (MQ=255)

                                             gTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTg                                                                                                >  1:6934/1‑151 (MQ=255)

                                              taataaTGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATCTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTgg                                                                                               >  1:69157/1‑151 (MQ=255)

                                                ataatGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGtt                                                                                             <  1:48624/151‑1 (MQ=255)

                                                            cTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATAc                                                                                 >  2:24324/1‑151 (MQ=255)

                                                                             tCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGgtg                                                                >  1:258113/1‑151 (MQ=255)

                                                                                     gCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTAGTTCGGCGGTCATACTGGTTAAATCGATGGTGTCATTTgg                                                        <  2:198044/151‑1 (MQ=255)

                                                                                        cGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTggg                                                       >  1:488258/1‑149 (MQ=255)

                                                                                          aaTTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCaaa                                                   <  2:126212/151‑1 (MQ=255)

                                                                                            tttAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATg                                                 >  2:219304/1‑151 (MQ=255)

                                                                                               aaCTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGtt                                               <  2:628488/150‑1 (MQ=255)

                                                                                                     cGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCAAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTAccc                                         >  2:419281/1‑150 (MQ=255)

                                                                                                      gCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCaa                                       <  1:567835/151‑1 (MQ=255)

                                                                                                       ccTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAAt                                      <  1:323313/151‑1 (MQ=255)

                                                                                                          aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCt                                   <  1:376168/151‑1 (MQ=255)

                                                                                                          aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCt                                   <  1:624594/151‑1 (MQ=255)

                                                                                                          aCGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATAATGGTTAAAGCGATGGGGTCATTTGGGCAAATGTTCTACCCAATGc                                    >  1:141851/1‑150 (MQ=255)

                                                                                                                 ggCAGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGa                              <  2:36683/149‑1 (MQ=255)

                                                                                                                    aGTGTGCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCta                          >  1:129611/1‑150 (MQ=255)

                                                                                                                       gtgCGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGGTAAAGCGATGGTGTCATTTGGGCAAATGGTCTACCCAATGCTGGTGAGCTATATg                      >  1:565552/1‑151 (MQ=255)

                                                                                                                          cGTAGGTATCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTg                   <  2:258106/151‑1 (MQ=255)

                                                                                                                                  tCGCTAACTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCaata            >  1:585875/1‑150 (MQ=255)

                                                                                                                                        aCTCAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTg     <  2:604188/151‑1 (MQ=255)

                                                                                                                                           cAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGt   <  1:611737/150‑1 (MQ=255)

                                                                                                                                           cAGCGTTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTa  <  1:492598/151‑1 (MQ=255)

GTGATCTCCGATAAGTTTGGTCGTCGGGCGGTGATATTAATGGCAGTAATAATGTATCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTA  >  NC_000913/1772719‑1773008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A