Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,836	T→C	L151P (CTG→CCG)	yeaI →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,836	0	T	C	100.0%	170.5 / NA	45	L151P (CTG→CCG)	yeaI	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/30); total (15/30)

CCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTT  >  NC_000913/1870689‑1870977

ccGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGAt                                                                                                                                            <  2:457022/151‑1 (MQ=255)

 cGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATc                                                                                                                                           <  1:343469/151‑1 (MQ=255)

        tttcttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACa                                                                                                                                     >  1:642140/1‑150 (MQ=255)

          tcttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACaaa                                                                                                                                   <  2:198854/150‑1 (MQ=255)

             tttcATGTTAATGAAATATTTCACTAACACACAAATTTTACCAACACTCAGTTTAAGCCTTGCATTTTTAAGTGGCCTTGTCGATTTAGTTCAAACCATTGTAAATATCCAAAAGCCAATTAACGGCAGTACACCGATCCAGTCAAAgg                                                                                                                                 <  1:130514/149‑2 (MQ=255)

             tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAg                                                                                                                                  <  2:305809/148‑1 (MQ=255)

             tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAg                                                                                                                                  <  2:364481/148‑1 (MQ=255)

             tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCg                                                                                                                               >  1:180231/1‑151 (MQ=255)

                   gTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatgat                                                                                                                         >  1:393083/1‑151 (MQ=255)

                                  cACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttctat                                                                                                          >  2:443630/1‑151 (MQ=255)

                                      aacaaacaAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                                                                      <  1:324288/151‑1 (MQ=255)

                                      aacaaacaAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                                                                      <  1:245651/151‑1 (MQ=255)

                                             aaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCa                                                                                               <  2:403184/151‑1 (MQ=255)

                                               aTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAAc                                                                                             >  2:16591/1‑151 (MQ=255)

                                                             cATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTAttt                                                                                 <  1:374132/149‑1 (MQ=255)

                                                                tttAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt                                                                             >  1:18407/1‑150 (MQ=255)

                                                                 ttAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTa                                                                            <  1:86894/150‑1 (MQ=255)

                                                                   aaGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAAcc                                                                         <  1:27221/151‑1 (MQ=255)

                                                                    aGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCt                                                                        <  2:145007/151‑1 (MQ=255)

                                                                         tGCATTTTTAAGTGGCCTTAACTATTTAGTTGAATCCATTGTAATTATCCATAAACCAATTAACGGCAGTGTTCCGATCCAGACAATGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTGTAACCGCGCt                                                                    <  1:595370/150‑1 (MQ=255)

                                                                                ttAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGctct                                                            >  1:504023/1‑151 (MQ=255)

                                                                                      ggCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGt                                                       <  2:670326/150‑1 (MQ=255)

                                                                                          ttATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATgg                                                  >  2:84063/1‑151 (MQ=255)

                                                                                           tATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATgg                                                  >  2:131096/1‑150 (MQ=255)

                                                                                           tATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATg                                                   <  2:542705/149‑1 (MQ=255)

                                                                                              cTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaaa                                               >  1:531604/1‑150 (MQ=255)

                                                                                                aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaaa                                               >  2:394111/1‑148 (MQ=255)

                                                                                                aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaa                                                <  1:332006/147‑1 (MQ=255)

                                                                                                aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaa                                                <  1:580806/147‑1 (MQ=255)

                                                                                                aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGa                                            <  1:402229/151‑1 (MQ=255)

                                                                                                       tGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACAt                                      <  2:374479/150‑1 (MQ=255)

                                                                                                        gAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATcc                                    <  1:96823/151‑1 (MQ=255)

                                                                                                                tGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaac                            >  1:32602/1‑151 (MQ=255)

                                                                                                                tGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaa                             <  2:177037/150‑1 (MQ=255)

                                                                                                                  tAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaaca                           >  2:225350/1‑150 (MQ=255)

                                                                                                                   aaTTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAAt                         <  2:540460/151‑1 (MQ=255)

                                                                                                                      tATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATaa                       <  2:224094/150‑1 (MQ=255)

                                                                                                                       aTCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGa                     <  2:454791/151‑1 (MQ=255)

                                                                                                                         ccATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaa                    >  1:90059/1‑150 (MQ=255)

                                                                                                                           aTAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaaaaa                 <  1:448398/151‑1 (MQ=255)

                                                                                                                              aaCCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAAc              <  2:137531/151‑1 (MQ=255)

                                                                                                                                  aaTTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCCATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACggg           >  1:444309/1‑150 (MQ=255)

                                                                                                                                     tAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAAt        <  2:530579/150‑1 (MQ=255)

                                                                                                                                        cGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTg    <  2:532675/151‑1 (MQ=255)

                                                                                                                                          gCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGtt  <  2:429675/151‑1 (MQ=255)

CCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTT  >  NC_000913/1870689‑1870977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A