Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,171	T→C	E2E (GAA→GAG)	yfcD ←	putative NUDIX hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,171	0	T	C	100.0%	177.9 / NA	46	E2E (GAA→GAG)	yfcD	putative NUDIX hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/29); total (17/29)

ATTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAT  >  NC_000913/2419025‑2419316

aTTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTcc                                                                                                                                                >  1:481218/1‑150 (MQ=255)

    tGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTa                                                                                                                                            <  1:510947/150‑1 (MQ=255)

                   gCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             <  1:303023/150‑1 (MQ=255)

                      cgacgaTGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTc                                                                                                                         >  1:681218/1‑151 (MQ=255)

                      cgacgaTGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTc                                                                                                                         <  2:469577/151‑1 (MQ=255)

                            tGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGgcgc                                                                                                                   >  2:255044/1‑151 (MQ=255)

                             gTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGct                                                                                                                  <  2:452305/151‑1 (MQ=255)

                               aaGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGctct                                                                                                                >  2:402657/1‑151 (MQ=255)

                                 gTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTa                                                                                                              <  2:69994/151‑1 (MQ=255)

                                  ttGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             <  1:138/135‑1 (MQ=255)

                                  ttGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             >  2:138/1‑135 (MQ=255)

                                     cACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACATTATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCGTGCgga                                                                                                          <  1:145471/151‑2 (MQ=255)

                                              gCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGa                                                                                                 <  1:434024/151‑1 (MQ=255)

                                                 gACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAATCGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAg                                                                                                <  1:606094/149‑1 (MQ=255)

                                                 gACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGtt                                                                                              <  1:7203/151‑1 (MQ=255)

                                                   cacTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTtgt                                                                                            <  2:397756/151‑1 (MQ=255)

                                                    acTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTtgtg                                                                                           <  2:574740/151‑1 (MQ=255)

                                                         gCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTaa                                                                                      <  1:37893/151‑1 (MQ=255)

                                                                 ttGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACgg                                                                               >  2:671847/1‑150 (MQ=255)

                                                                    ttCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGAtt                                                                            >  1:665004/1‑150 (MQ=255)

                                                                         ggctggctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATcgc                                                                      >  1:292909/1‑151 (MQ=255)

                                                                              gctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCt                                                                 <  1:644278/151‑1 (MQ=255)

                                                                               ctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTg                                                                >  2:447929/1‑151 (MQ=255)

                                                                                             tCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                  >  2:90192/1‑151 (MQ=255)

                                                                                              cGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                  <  2:121343/150‑1 (MQ=255)

                                                                                              cGTTCTCTTCATTGACAATATCCACCCATTCAGTACGTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTTTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTg                                                 <  1:213036/151‑1 (MQ=255)

                                                                                                      tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGTAAACCTTCTTTTTCTGGCGCTCTTACGTCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAt                                            <  1:347556/148‑1 (MQ=255)

                                                                                                      tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAtt                                           <  1:455851/149‑1 (MQ=255)

                                                                                                      tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTg                                          >  1:165793/1‑150 (MQ=255)

                                                                                                       cATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAg                                        <  1:446901/151‑1 (MQ=255)

                                                                                                        aTTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGt                                       <  2:69739/151‑1 (MQ=255)

                                                                                                              aaTATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGAtt                                 <  1:255040/151‑1 (MQ=255)

                                                                                                                  tCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTacac                             >  2:117589/1‑151 (MQ=255)

                                                                                                                    cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACt                            >  2:370534/1‑150 (MQ=255)

                                                                                                                    cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACt                            >  2:500624/1‑150 (MQ=255)

                                                                                                                    cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTAAGTGCGATTACACt                            >  1:283833/1‑150 (MQ=255)

                                                                                                                      cccATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAg                         <  1:500653/151‑1 (MQ=255)

                                                                                                                      cccATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTGTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTaa                          <  1:487132/150‑1 (MQ=255)

                                                                                                                           tCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACg                     <  2:178044/150‑1 (MQ=255)

                                                                                                                            cAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTc                   <  1:276609/151‑1 (MQ=255)

                                                                                                                              gTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAt                 <  2:295730/151‑1 (MQ=255)

                                                                                                                               tACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAtt                >  2:631675/1‑151 (MQ=255)

                                                                                                                                   tGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATcc            >  1:554403/1‑151 (MQ=255)

                                                                                                                                        aaCGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCAt       <  1:665492/151‑1 (MQ=255)

                                                                                                                                            acgCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCa   <  2:555030/151‑1 (MQ=255)

                                                                                                                                             cgCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCa   <  1:473017/150‑1 (MQ=255)

                                                                                                                                              gctgctCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAt  >  2:663207/1‑150 (MQ=255)

ATTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAT  >  NC_000913/2419025‑2419316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A