Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I1 R1 141 76.7 2402765 98.4% 2364320 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 949,117 (T)5→4 coding (452/909 nt) ycaN ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913949,1130T.91.9% 344.0 / 16.7 87coding (456/909 nt)ycaNLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (7/0);  new base . (39/40);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 1.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGTG  >  NC_000913/948964‑949258
                                                                                                                                                     |                                                                                                                                                  
aaaGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATTGCCACGCCTATCCTAtttt                                                                                                                                                   >  1:806984/1‑151 (MQ=255)
       tGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCttttcgac                                                                                                                                             <  2:76341/150‑5 (MQ=255)
       tGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCttttcgac                                                                                                                                             <  2:922128/150‑5 (MQ=255)
              gggATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCaa                                                                                                                                     <  2:962012/150‑1 (MQ=255)
              gggATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACCCTGATCATATCTTTTT‑GACGATGCaa                                                                                                                                     >  1:631425/1‑150 (MQ=255)
                gATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTa                                                                                                                                  <  1:350000/151‑1 (MQ=255)
                 aTAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTT‑GACGATGCAACTa                                                                                                                                  >  1:616066/1‑150 (MQ=255)
                    aCGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGTCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTc                                                                                                                              >  2:629027/1‑151 (MQ=255)
                    aCGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTc                                                                                                                              >  2:951535/1‑151 (MQ=255)
                     cGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCg                                                                                                                             <  1:453987/151‑1 (MQ=255)
                      gAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTT‑GACGATGCAACTAAGTCg                                                                                                                             >  1:749012/1‑150 (MQ=255)
                      gAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCg                                                                                                                             >  2:659001/1‑150 (MQ=255)
                      gAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGa                                                                                                                            <  2:1116328/151‑1 (MQ=255)
                        aaaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAAc                                                                                                                          <  2:963455/151‑1 (MQ=255)
                        aaaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAAc                                                                                                                          <  1:1014456/151‑1 (MQ=255)
                        aaaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAAc                                                                                                                          <  2:695993/151‑1 (MQ=255)
                        aaaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCCCCACGCTGATCATATC‑TTTTCCACGATGCAACCAAGTCGaa                                                                                                                           >  1:1017496/1‑150 (MQ=255)
                         aaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGaa                                                                                                                           >  2:45460/1‑149 (MQ=255)
                         aaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAAc                                                                                                                          >  2:1181125/1‑150 (MQ=255)
                         aaaCAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACg                                                                                                                         <  1:737434/151‑1 (MQ=255)
                          aacaacACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGaa                                                                                                                           >  2:545223/1‑148 (MQ=255)
                             aacaCATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAg                                                                                                                     <  2:1007804/151‑1 (MQ=255)
                                acaTTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCg                                                                                                                   >  2:85792/1‑150 (MQ=255)
                                acaTTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑t                                                                                                                 <  2:336047/151‑1 (MQ=255)
                                  aTTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCCCGCTGATCATATCTTTTT‑GACGATGCAACTAAGTCGAACGCCAACg                                                                                                                   >  1:952659/1‑148 (MQ=255)
                                      atgatTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTT‑GACGATGCCACTAAGTCGAACGCCAGCG‑TCaaa                                                                                                             >  2:722246/1‑149 (MQ=255)
                                           tAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCCCGCTGGTCATATCTTTTT‑GACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGccc                                                                                                         >  1:13641/1‑148 (MQ=255)
                                            aaGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGtt                                                                                                     >  2:661447/1‑151 (MQ=255)
                                                 gATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACg                                                                                                >  1:451174/1‑151 (MQ=255)
                                                  aTCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACg                                                                                                >  2:788000/1‑150 (MQ=255)
                                                  aTCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGa                                                                                               <  2:257833/151‑1 (MQ=255)
                                                   tCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGGACGCCACGCTTTACCTATC‑TTTTCGACCATGGCACTAAGTCTGACGCCCACG‑TCAAAGACCCGTTGGAc                                                                                                 >  1:1152041/1‑148 (MQ=255)
                                                    cATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGat                                                                                              >  1:808593/1‑150 (MQ=255)
                                                       gAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCg                                                                                          <  1:441260/151‑1 (MQ=255)
                                                          gATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACa                                                                                       <  1:959925/151‑1 (MQ=255)
                                                             ggCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAag                                                                                     <  1:906445/150‑1 (MQ=255)
                                                              gCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAagag                                                                                   <  1:709753/151‑1 (MQ=255)
                                                                  ggTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAgtc                                                                               <  1:1155727/151‑1 (MQ=255)
                                                                    tttGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAgtcg                                                                              >  2:144787/1‑150 (MQ=255)
                                                                        ccATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATAT‑ATTTTCGACGATGCAACTAAGGCGAACGCCATCG‑TCAAAGCCCTGTTGGACGATATCGGCAAGAGAGGCGTCAg                                                                          >  2:1201340/1‑150 (MQ=255)
                                                                         cATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGtt                                                                        <  1:977451/151‑1 (MQ=255)
                                                                         cATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGtt                                                                        <  1:98394/151‑1 (MQ=255)
                                                                          atatCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGGCGATCGCCACCCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCCGTTTGACGATATCGACAAGAGAGTCGTCCGtt                                                                        >  1:809261/1‑150 (MQ=255)
                                                                             tCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGACCGCCACGCCTATCATATCTTTTTGGA‑GATTCAACTTCATCGGACGCCAGCGCTCAATCCCCTTTTTG‑CGGTATACACAAAAGAGTCCTCCCTtgtg                                                                     >  1:577920/1‑150 (MQ=255)
                                                                              cTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTtgtg                                                                     >  2:178373/1‑149 (MQ=255)
                                                                                 gCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCt                                                                 <  2:661739/150‑1 (MQ=255)
                                                                                    aaGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGAc                                                             <  1:179874/151‑1 (MQ=255)
                                                                                    aaGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGACGAACGCCACGCTGGTCATATC‑CTTTCCAAGAAGCAACTAAATCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGAAATCGACAAGAAAGTCGTCAGGTGTGAGCCCGa                                                              >  1:50988/1‑150 (MQ=255)
                                                                                     aGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCCATCGCCACGCTGCGCCAATC‑TTTTCGACGAATCTAAAAAATCGAACGCCAGCG‑CCAAAGCCCTCTTTGTGGATATCGACAATAGAGTCGTCAGTTGTGGGGTCgcgt                                                            >  1:618488/1‑148 (MQ=255)
                                                                                      gTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACtt                                                           >  2:741334/1‑151 (MQ=255)
                                                                                      gTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACtt                                                           <  2:675952/151‑1 (MQ=255)
                                                                                             ggCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACCCTGATCATATC‑TTTTCGACGATGCAACAAAGTCGAAAGCCAGCG‑TCAAAACCCTCTTTGACGATTTCGGAAAGAGAGTCGTCTGTTGTGAGGTCGACTTTTatat                                                     >  2:569729/1‑150 (MQ=255)
                                                                                              gCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCa                                                   <  1:341110/151‑1 (MQ=255)
                                                                                                 ttGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAATCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAAAGAGTCGTCAGTTGTGAGCTCGACTTTTATATCAgg                                                 >  2:143657/1‑150 (MQ=255)
                                                                                                   gctgctACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGat                                               >  2:209823/1‑150 (MQ=255)
                                                                                                   gctgctACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGat                                               <  1:464514/150‑1 (MQ=255)
                                                                                                   gctgctACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGat                                               <  2:911620/150‑1 (MQ=255)
                                                                                                      gctACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGatat                                             <  1:376688/149‑1 (MQ=255)
                                                                                                      gctACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATAtt                                            <  1:579726/150‑1 (MQ=255)
                                                                                                           acaTAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACg                                        >  2:1187834/1‑149 (MQ=255)
                                                                                                               aaTTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGaa                                  <  2:35199/151‑1 (MQ=255)
                                                                                                                aTTTCACTGGCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGaaa                                 <  1:226403/151‑1 (MQ=255)
                                                                                                                        ggCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAaaccaacc                           <  2:800156/149‑1 (MQ=255)
                                                                                                                        ggCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAaaccaacc                           >  2:1003840/1‑149 (MQ=255)
                                                                                                                        ggCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAg                         <  2:736530/151‑1 (MQ=255)
                                                                                                                        ggCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAg                         <  1:370709/151‑1 (MQ=255)
                                                                                                                         gCGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCGACCAGt                        <  2:917107/151‑1 (MQ=255)
                                                                                                                          cGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGt                        >  2:972682/1‑150 (MQ=255)
                                                                                                                          cGGGCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTa                       <  1:609751/151‑1 (MQ=255)
                                                                                                                            ggCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTaaa                     <  2:635551/151‑1 (MQ=255)
                                                                                                                             gCCGATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAg                    <  1:478874/151‑1 (MQ=255)
                                                                                                                                gATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGAc                  >  1:1007123/1‑150 (MQ=255)
                                                                                                                                gATCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACa                 <  2:1183607/151‑1 (MQ=255)
                                                                                                                                 aTCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACAt                >  2:234696/1‑151 (MQ=255)
                                                                                                                                 aTCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACAt                <  2:862370/151‑1 (MQ=255)
                                                                                                                                  tCGCCACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACAtt               >  1:156434/1‑151 (MQ=255)
                                                                                                                                     ccACGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTaa             >  2:176964/1‑150 (MQ=255)
                                                                                                                                        cGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAat         >  2:503667/1‑151 (MQ=255)
                                                                                                                                        cGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAat         >  1:1144394/1‑151 (MQ=255)
                                                                                                                                        cGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAat         >  1:552215/1‑151 (MQ=255)
                                                                                                                                        cGCTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAat         >  2:1062334/1‑151 (MQ=255)
                                                                                                                                          cTGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAatat       >  2:719958/1‑151 (MQ=255)
                                                                                                                                           tGATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAatat       >  1:35715/1‑150 (MQ=255)
                                                                                                                                            gATCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATAc     >  1:299327/1‑151 (MQ=255)
                                                                                                                                             aTCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACg    >  2:853919/1‑151 (MQ=255)
                                                                                                                                             aTCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACg    >  2:1116121/1‑151 (MQ=255)
                                                                                                                                              tCATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGt   >  2:317236/1‑151 (MQ=255)
                                                                                                                                               cATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGta  <  1:195243/151‑2 (MQ=255)
                                                                                                                                               cATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGt   <  1:348178/150‑1 (MQ=255)
                                                                                                                                               cATATC‑TTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGTg  <  2:83921/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGATGCAACTAAGTCGAACGCCAGCG‑TCAAAGCCCTGTTGGACGATATCGACAAGAGAGTCGTCAGTTGTGAGCTCGACTTTAATATCAGGATATTCACGTGTGAAACCAACCAGTAAAGACATTAAAAATATACGTG  >  NC_000913/948964‑949258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A