Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I1 R1	141	76.7	2402765	98.4%	2364320	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,560,722	A→G	Y70H (TAC→CAC)	ddpB ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,560,722	0	A	G	100.0%	181.2 / NA	50	Y70H (TAC→CAC)	ddpB	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/28); total (22/28)

GCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGC  >  NC_000913/1560578‑1560865

gCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTa                                                                                                                                           <  1:275604/151‑1 (MQ=255)

gCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTa                                                                                                                                           <  1:449863/151‑1 (MQ=255)

   ggATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTaaa                                                                                                                                         >  1:266996/1‑150 (MQ=255)

       gCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACt                                                                                                                                      >  2:737724/1‑149 (MQ=255)

       gCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGt                                                                                                                                    <  2:175916/151‑1 (MQ=255)

           aTCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGtacat                                                                                                                                <  1:746859/151‑1 (MQ=255)

             cAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGtacata                                                                                                                               >  2:646216/1‑150 (MQ=255)

              aGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGtacata                                                                                                                               >  2:761392/1‑149 (MQ=255)

                gAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGt                                                                                                                           <  1:905909/151‑1 (MQ=255)

                 aGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTg                                                                                                                          <  1:340705/151‑1 (MQ=255)

                    gCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCt                                                                                                                       <  2:219350/151‑1 (MQ=255)

                        gcagcagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTc                                                                                                                   <  1:882878/151‑1 (MQ=255)

                          agcagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTc                                                                                                                   <  2:653240/149‑1 (MQ=255)

                                gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                           <  1:1078883/151‑1 (MQ=255)

                                      aaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCAAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAAATGTACATACAATGGCTTGTCCAAGCCCACtttgt                                                                                                     >  1:307805/1‑148 (MQ=255)

                                          ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                 <  1:19370/151‑1 (MQ=255)

                                                       cGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACg                                                                                    <  1:369044/151‑1 (MQ=255)

                                                        gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGa                                                                                   <  1:854043/151‑1 (MQ=255)

                                                              aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                                                             <  1:204308/151‑1 (MQ=255)

                                                              aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGCGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTTCACGAAAGCa                                                                              >  1:179332/1‑150 (MQ=255)

                                                               aaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCAGTTTCTGGCGAAAATTTTTCAccaaaacat                                                                             >  1:472526/1‑142 (MQ=255)

                                                                  atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCt                                                                         <  2:539464/151‑1 (MQ=255)

                                                                  atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCt                                                                         <  2:1184686/151‑1 (MQ=255)

                                                                  atatACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGATTCTGGGGAATATTTTcctc                                                                                     >  2:221049/1‑137 (MQ=255)

                                                                    ataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTg                                                                        <  1:578631/150‑1 (MQ=255)

                                                                     taCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTTCACGAAAGCATCGCTgg                                                                       >  2:214615/1‑150 (MQ=255)

                                                                      aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                      <  2:774000/150‑1 (MQ=255)

                                                                      aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                      <  1:996727/150‑1 (MQ=255)

                                                                      aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                      <  1:274243/150‑1 (MQ=255)

                                                                          aaCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGt                                                                 <  2:266999/151‑1 (MQ=255)

                                                                              ctTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCAGGCCCCGTTGCTGGGGGAAATTTTTCACGATAACATCCCTGGCGCGGTTAcc                                                             >  1:516254/1‑151 (MQ=255)

                                                                                tCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAccc                                                            <  2:672373/150‑1 (MQ=255)

                                                                                tCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAccc                                                            <  1:960830/150‑1 (MQ=255)

                                                                                    gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                       <  2:14617/151‑1 (MQ=255)

                                                                                             gCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAAcgcg                                               >  2:1149761/1‑150 (MQ=255)

                                                                                                    aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCAGGTGCTGGCGGATATTTTTCCCCAAAGCATCGCTTGCGCGGTCACCCCCCCGGAAACGCGcccggtc                                        >  1:137889/1‑145 (MQ=255)

                                                                                                            aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGGCACCCGCCCGTAACCGCGCCGGATCACCAggg                                 >  2:366009/1‑149 (MQ=255)

                                                                                                                  ccAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAAAATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                           >  2:1114814/1‑149 (MQ=255)

                                                                                                                            aTGAAACAGGTCGCTGACGTGGCGGTAAAACTTTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCAAATATTTCCCACGATAGCATCGCTGGGGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                <  1:779933/150‑1 (MQ=255)

                                                                                                                               aaaCAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCCCTGGCGCGGGCACCCCCCCGTAAACGCGCCGGATCACCAGGGGTCAGATGCGAGATAATaaa             >  2:573100/1‑150 (MQ=255)

                                                                                                                               aaaCAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCCGGTGCTGGGGAAAATTTTTCCCGATAGCATCGCTGGCGCGGGGACCCGCCCGTAAACGCGCCCGGTCACCACGGGTCAGATGCGGGATAATaaa             >  1:705876/1‑150 (MQ=255)

                                                                                                                                aaCAGGTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCCGGCGAAAATTTTCCACGATAACAACCCCTGCCCGGGCACCCCCCCGTAAACGCGCCCGGACACCAGGGATCAGATGCGGGATAATaaa             >  1:573452/1‑149 (MQ=255)

                                                                                                                                    ggTCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCAGTTGCTGGGGAATATTTTTCACGATAACATCGCTGGCGCGGGCCCCCCCCCGGAAACGCGCCCGGTCACCAGGGGTCAGATGCGGGATAATAAAAGTaa        >  1:926118/1‑150 (MQ=255)

                                                                                                                                      tCGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCCATCCCCCTTTCTGGCGGAAATTTTTCACGATAAAAACCCCGTCGCGGGCACCCCCCCGGAACCCCGCCCGGTCCCCCGGGGTCCGAATCGAGGGAATaaaacgca        >  1:746505/1‑143 (MQ=255)

                                                                                                                                       cGCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCCGTCCCCGTTGCTGGCGAATATTTTCCACGATAACCTCGCTGGCGCGGTCACCCCCCAGTAACCGCCCCGGATCACCAGGGGTCAGATGCGGGGTAATaaaaggaa        >  2:1140899/1‑142 (MQ=255)

                                                                                                                                       cGCTGACGTGGCGGAAAACCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCAAATATTTTCCAGGATAGCATCGCTGGCGGGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTa         <  1:784515/146‑1 (MQ=255)

                                                                                                                                        gCTGAGGTGGCGGTAAAACTTTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCAc    <  2:464886/150‑1 (MQ=255)

                                                                                                                                        gCTGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCAAGTTGCTGGCAAATTTTTTCCACGATAGCTTCGCTGGCCCGGCCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa     <  2:724293/149‑1 (MQ=255)

                                                                                                                                          tGACGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGGGGATATTTTTCACGATAGCATCGCCGGCGCGGTCACCCGCCCGTAAACGCGCCGGATCACCAGGGGTCAGATGCGAGATAATAAAAGTAATCACGc  >  1:54892/1‑150 (MQ=255)

                                                                                                                                             cGTGGCGGTAAAACTGTACATACAGTGGCTGGTCCCCTCCCCGTTGCTGGCGGATATTTTTCACGCTAGCAAGCCTGGCGCGGGGACCCCCCCGGAAACTCCCCGGCTCCCCCGGGGTCAAATGCGGGAAAATACAAGTCATCAccc  >  1:165218/1‑145 (MQ=255)

GCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGC  >  NC_000913/1560578‑1560865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A