Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,033	A→G	V234A (GTG→GCG)	dosP ←	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,033	0	A	G	100.0%	139.7 / NA	39	V234A (GTG→GCG)	dosP	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/19); total (20/19)

CGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTC  >  NC_000913/1564887‑1565179

cGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTc                                                                                                                                                >  2:307173/1‑151 (MQ=255)

  aTGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCAt                                                                                                                                              >  1:368720/1‑151 (MQ=255)

        cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        <  2:521201/151‑1 (MQ=255)

        cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        <  1:402735/151‑1 (MQ=255)

        cGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCg                                                                                                                                        >  2:338546/1‑151 (MQ=255)

          aCAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCggg                                                                                                                                      <  2:569933/151‑1 (MQ=255)

                   ttCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATag                                                                                                                               >  2:59492/1‑149 (MQ=255)

                   ttCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATag                                                                                                                               >  1:53856/1‑149 (MQ=255)

                            ttCATGAAATGGCGTGCTGCTGCACATTGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGCTCTTTGGTGAAATCCCCGAAAGTCATTACAAGGTTCTTCAGATGTGCGAGCGCGTCATAAACCGGGCTGATAAAGGCTTTGATc                                                                                                                    >  2:447320/1‑151 (MQ=255)

                            ttCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAAt                                                                                                                     <  2:202473/150‑1 (MQ=255)

                              cATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATcc                                                                                                                   >  2:497063/1‑150 (MQ=255)

                                     tGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAttttt                                                                                                           >  2:546482/1‑151 (MQ=255)

                                      ggCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAtttttt                                                                                                          <  2:300282/151‑1 (MQ=255)

                                      ggCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGAtttttt                                                                                                          <  2:272417/151‑1 (MQ=255)

                                        cGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTc                                                                                                         >  1:112416/1‑150 (MQ=255)

                                           gctgctGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCg                                                                                                     <  1:328491/151‑1 (MQ=255)

                                                              agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGaa                                                                                  <  2:331173/151‑1 (MQ=255)

                                                              agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGa                                                                                   >  2:604785/1‑150 (MQ=255)

                                                              agaATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGa                                                                                   >  1:421711/1‑150 (MQ=255)

                                                                      gCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTc                                                                           <  1:110425/150‑1 (MQ=255)

                                                                       ccTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCt                                                                         >  1:9586/1‑151 (MQ=255)

                                                                                   cGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                             <  1:419524/151‑1 (MQ=255)

                                                                                    gAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCCTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                             >  2:507887/1‑150 (MQ=255)

                                                                                      aTCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGttt                                                          >  1:379155/1‑151 (MQ=255)

                                                                                          gCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCa                                                      <  1:576527/151‑1 (MQ=255)

                                                                                           ccGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCAt                                                     <  2:308430/151‑1 (MQ=255)

                                                                                                  tcGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAAc                                               <  1:259855/150‑1 (MQ=255)

                                                                                                       gATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                         >  2:147448/1‑151 (MQ=255)

                                                                                                       gATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                         <  1:307180/151‑1 (MQ=255)

                                                                                                            ccGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACg                                    >  2:50163/1‑151 (MQ=255)

                                                                                                                gaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                                <  2:198143/151‑1 (MQ=255)

                                                                                                                 aaaGTCATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGc                               <  1:211998/151‑1 (MQ=255)

                                                                                                                      cATTACCAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGtt                           >  1:480290/1‑150 (MQ=255)

                                                                                                                            cAGGTTCTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                    <  1:546493/151‑1 (MQ=255)

                                                                                                                                  cTGCAGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt              >  2:336827/1‑151 (MQ=255)

                                                                                                                                      aGATGCGCGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg          <  1:563398/151‑1 (MQ=255)

                                                                                                                                          gcgcGAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAAt      <  1:416169/151‑1 (MQ=255)

                                                                                                                                              gAGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGtt   >  1:592755/1‑150 (MQ=255)

                                                                                                                                               aGCGCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTc  >  2:306461/1‑150 (MQ=255)

CGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTC  >  NC_000913/1564887‑1565179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A