Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,560,722	A→G	Y70H (TAC→CAC)	ddpB ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,560,722	0	A	G	100.0%	104.4 / NA	33	Y70H (TAC→CAC)	ddpB	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/22); total (11/22)

CCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAG  >  NC_000913/1560575‑1560867

ccAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCg                                                                                                                                                 <  2:835119/151‑1 (MQ=255)

          gCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTg                                                                                                                                       >  2:172523/1‑150 (MQ=255)

              aTCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGtaca                                                                                                                                   >  1:579744/1‑150 (MQ=255)

                     gTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTgg                                                                                                                           <  1:634451/151‑1 (MQ=255)

                       gCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCt                                                                                                                         <  2:574404/151‑1 (MQ=255)

                       gCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCt                                                                                                                         <  2:347795/151‑1 (MQ=255)

                                   gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                             <  2:452101/151‑1 (MQ=255)

                                   gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                             <  1:734926/151‑1 (MQ=255)

                                      ccAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCCCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                                                          <  1:870973/151‑1 (MQ=255)

                                             ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                   >  1:499401/1‑151 (MQ=255)

                                             ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                   <  1:725725/151‑1 (MQ=255)

                                                 cTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGGCCCGGCCCCGTTGCTGGCGaa                                                                                                 >  2:104678/1‑149 (MQ=255)

                                                     aGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCCGGGCCCGGCCCCGTTTCTTGGGGAAAtttt                                                                                           >  2:48690/1‑151 (MQ=255)

                                                        gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                                                        <  1:316096/151‑1 (MQ=255)

                                                            ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                                                                    <  2:824573/151‑1 (MQ=255)

                                                               ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTTCACGATAg                                                                                  >  1:818414/1‑150 (MQ=255)

                                                               ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                                                                 <  1:595290/151‑1 (MQ=255)

                                                                 aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                                                               <  2:975879/151‑1 (MQ=255)

                                                                         aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                        <  1:745760/150‑1 (MQ=255)

                                                                          cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCCGGCGAATATTTTCCACGATAGCATCGCTGgcgc                                                                      <  2:420896/151‑1 (MQ=255)

                                                                            cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                                                                    <  1:754872/151‑1 (MQ=255)

                                                                                        cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCCATCCCCCGTGCCGGCGCAAATTTTCCACAACAGCCTTGCTTGCGCCGTCAcccccc                                                           >  2:107082/1‑148 (MQ=255)

                                                                                                  ccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGGCCAGGCCCCGGTGCTGGCGGATATTTTTCACCAAAACATCGCCGGCGCGGGCCCCCGCCCGTAACCCCGcc                                               >  2:391418/1‑150 (MQ=255)

                                                                                                      tccGAATGGATTTCCCCAGGTCCCCAGGAACCGGGTCTGTGA‑GGGGGGGTAAAACCTGTAAATACAGTGGCTGGTCAAGTCCCAGTTCCGGCCAAATATTTTCCAGTAATGCTTGCCTGGGGCGGCCTCCCGCCAGTAACCGCGCCg                                              <  2:514122/145‑1 (MQ=255)

                                                                                                                tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                 <  1:324031/150‑1 (MQ=255)

                                                                                                                  ttCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGGGGCTGGTCCCATGCCAATTTCTTGCGAATATTTTCCCAGAAAACATCGCCGGCCCCGACACCCCCCAGAAAACCCCCCGCATCACCCggg                                   >  2:422208/1‑146 (MQ=255)

                                                                                                                   tCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAATCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTTGCCCGGTCACCCGCCCGTAACCGCGCCGGATCACCCGGGATc                                >  1:909005/1‑148 (MQ=255)

                                                                                                                     caaGGACACCATGAAACAGGTCGCGGACGTGGGGGAAAAA‑CTGTACATACAGGGGCTGGCCCAGCCCCAGTTGCTGCCGAATATTTTCCACGATAGCACCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCa                               <  2:469346/145‑1 (MQ=255)

                                                                                                                           cACCATGAAACGGGTCTCTGTCGGGGCTGTAAAA‑TTGTACAGACGGTGGCGGGTCCATTCCCATTTCCTGACGATTTTTTTCCACAATACCAACGTGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgaga                      <  2:632779/150‑1 (MQ=255)

                                                                                                                              cATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGCACATACAGTGGCTGGGCCACACCCCGTTGCTGGGGAAAATTTTCCACGAAAGGAACGCCGGGGCGGTGAACCCCCCGAAAACCCGCCCGATCCCCAGGGacacaaaacgagaga                    >  1:100655/1‑135 (MQ=255)

                                                                                                                                    acgggTCGCTGACGTGGCGGAAAAA‑TTGTCCATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATACCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGt            <  1:520424/148‑1 (MQ=255)

                                                                                                                                       ggTCGCTGACGTGGCGGTAAAC‑CTGTACATACAGGGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAAt         <  1:905995/151‑1 (MQ=255)

                                                                                                                                              gACGTGGCGGAAAAC‑CTGTACAAACAGTGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCGCGATAGCATCGCTGGGGCGGTCAACCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAg  <  2:439411/151‑1 (MQ=255)

CCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAG  >  NC_000913/1560575‑1560867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A