Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,436,536	A→G	Y120H (TAC→CAC)	ompN ←	outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,436,536	0	A	G	100.0%	116.8 / NA	33	Y120H (TAC→CAC)	ompN	outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

GCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT  >  NC_000913/1436388‑1436673

gcgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCa                                                                                                                                         <  2:4215/151‑1 (MQ=255)

 cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                        >  1:200124/1‑151 (MQ=255)

 cgAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAt                                                                                                                                        >  2:600644/1‑151 (MQ=255)

  gAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCAtt                                                                                                                                       >  1:49659/1‑151 (MQ=255)

    aGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTAc                                                                                                                                     <  2:302634/151‑1 (MQ=255)

     gTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACg                                                                                                                                    <  1:86207/151‑1 (MQ=255)

         aGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCAt                                                                                                                                >  2:250063/1‑151 (MQ=255)

              aTTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTa                                                                                                                           <  1:204852/151‑1 (MQ=255)

                 tACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGt                                                                                                                        >  2:263955/1‑151 (MQ=255)

                  aCCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGt                                                                                                                        <  2:359450/150‑1 (MQ=255)

                  aCCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTc                                                                                                                       <  2:492979/151‑1 (MQ=255)

                         cGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATc                                                                                                                <  2:394772/151‑1 (MQ=255)

                         cGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAAGCAGAATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTaa                                                                                                                  >  2:162485/1‑149 (MQ=255)

                                aTCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGa                                                                                                          >  2:401288/1‑150 (MQ=255)

                                              aaGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCaa                                                                                            <  1:567740/150‑1 (MQ=255)

                                                  cgcgACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAAttt                                                                                        <  2:305277/150‑1 (MQ=255)

                                                   gcgACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCa                                                                                      <  1:362250/151‑1 (MQ=255)

                                                         cgaTTGGCTCGACCAGTCCTCAAGGTGTCTTCATTGGTATAAGAGTCACCGCCAAATTCAGGCTGCATATCGGACCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCa                                                                                      >  2:551583/3‑145 (MQ=255)

                                                                  cGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGc                                                                         <  1:500973/149‑1 (MQ=255)

                                                                  cGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCa                                                                       <  2:588306/151‑1 (MQ=255)

                                                                   gACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAg                                                                      <  2:239856/151‑1 (MQ=255)

                                                                            tAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTcc                                                              >  1:22469/1‑150 (MQ=255)

                                                                               aGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGa                                                          <  1:6298/151‑1 (MQ=255)

                                                                                         aTTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGtttttt                                                >  1:4750/1‑151 (MQ=255)

                                                                                           tGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTg                                               <  1:215761/150‑1 (MQ=255)

                                                                                                        aCCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAgtgt                                   <  1:301816/149‑1 (MQ=255)

                                                                                                           gCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGACGTGCATTACGCCATAATTACGTCCGTAATCGAAATAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATCACTGGTTTTTTGAAGATTCAGTGTTGttt                              <  2:397062/151‑1 (MQ=255)

                                                                                                                     ggCAGCATATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAatat                    >  1:603527/1‑151 (MQ=255)

                                                                                                                       cagcaTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTg                  >  2:464379/1‑151 (MQ=255)

                                                                                                                          caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  2:103878/1‑151 (MQ=255)

                                                                                                                          caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  1:357704/1‑151 (MQ=255)

                                                                                                                          caTATCGGTCCAGCCTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTAt               >  1:219730/1‑151 (MQ=255)

                                                                                                                                        cTTCGATGTCGTGCATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGt  >  2:123978/1‑150 (MQ=255)

GCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGACGCCATTGGCTCGACCAGTCATAAAGTTGTCTGCATTGGTATAAGAGTCACCGCCAAATTCAGGCAGCATATCGGTCCAGCCTTCGATGTCGTACATTACGCCATAATTACGTCCGTAATCGAAAGAACCGTAATCTGCAAATTTCAGCCCGGCAAATGCCAGACGGGTCCATGACTGGTTTTTTGAAGATTCAGTGTTGTTTGCCTGAATATTGTATTCCCATTGACCGT  >  NC_000913/1436388‑1436673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A