Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I1 R1 170 39.5 1266514 99.2% 1256381 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,029,338 G→A T10M (ACG→ATG)  yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,029,3380GA97.6% 149.2 / NA 42T10M (ACG→ATG) yedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (18/23);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAG  >  NC_000913/2029193‑2029485
                                                                                                                                                 |                                                                                                                                                   
gggAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGt                                                                                                                                                <  2:412379/151‑1 (MQ=255)
  gAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTa                                                                                                                                               >  1:362488/1‑150 (MQ=255)
          cGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTcc                                                                                                                                      <  1:556422/151‑1 (MQ=255)
                      aCGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTTGCCGCCAGTTTGCCCATCACCGAGATATTGTCCAGGAGTGTGGGGATATCATCGAGCAAAATCAGTAAAATACTCCCGGCCaaaa                                                                                                                             >  2:225488/1‑148 (MQ=255)
                        gCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTTGCCGCCAGTTTGCCCCTCCCGGAGATATCGTCCCGGAGTGTGGCGATATCATCGAGCAACATCAGGAAACCACTCCCCGCCAAAAGCAtt                                                                                                                        >  1:342889/1‑151 (MQ=255)
                         ccTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCCGTTTTCCCATAACGGAGATAATGTCCAGGAGTGTGGCGATATCATTCCGCAACAACAGTAAACTACTCCCGGCCAAAAACact                                                                                                                        >  1:585762/1‑148 (MQ=255)
                                  tgttgCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCa                                                                                                                <  1:317392/149‑1 (MQ=255)
                                   gttgCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAtt                                                                                                              <  1:481689/150‑1 (MQ=255)
                                     tgCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAtttt                                                                                                            <  1:453060/150‑1 (MQ=255)
                                      gcgcATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAttttt                                                                                                           <  2:165784/150‑1 (MQ=255)
                                       cgcATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCCCCGAGATAACGTCCAGGAGGGTGGCGATATCATCGAGCAACATCAGTAAAATACTCCCCGCCAAAAGCATTCCCTCTCCttttttt                                                                                                         >  2:58050/1‑149 (MQ=255)
                                             gAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGGCCATCACGGAGATATCGTCCAGGAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAACATTTCCTTTCATTTTTTttgtt                                                                                                     >  2:343479/1‑149 (MQ=255)
                                             gAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTAttgttg                                                                                                    <  1:621456/150‑1 (MQ=255)
                                                cGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAgg                                                                                                <  1:320660/151‑1 (MQ=255)
                                                      gTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACCGAGATATCGTCCCGGAGTGTGGCGATATCATCGAGCAACATCAGTAAAATACTCCCGGGCAAAAGCATTTCCTCTCCTTTTTATTGTTGAAggggg                                                                                             >  2:381708/1‑146 (MQ=255)
                                                       tCATCCCCTAATACACCGGCGGTTTTTTTCGCCGCCCGTTTTCCCCTCACGGAGATATCGTCCCGTAGTGTGGCGGTATCATCGAGCAACAACAGTAAACTACTCCCCGCCAAAAACATTCCCTCTCATTTTTATTGTTGAAggggg                                                                                             >  1:96835/1‑145 (MQ=255)
                                                       tCATCCCCTAATACACCGGCGGTTTTCTTTGCCGCCCGTTTTCCCATCACGGAGATATTGTCCCGTAATTTGGCGATATCATCGAGCAACATCAGTAAAATACTCCCCGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAggggg                                                                                             >  1:550088/1‑145 (MQ=255)
                                                       tCATCCCCTAATACACCGGCGGTTTTCTTTCCCCCCAGTTTGCCCCTTACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTTCCTCTCATTTTTATTGTTGAAggg                                                                                               >  1:419503/1‑145 (MQ=255)
                                                        cATCCCCTAATACACCGGCGGTTTTCTTCGCCCCCCGTTTGCCCATCACCGAGATATCGGCCAGTAGTGTGGCGGTATCATCGAGCAACATCAGTAAACTACTCCCCGCCAAAAGCATTCCCTCTCCTTTTTTTTTTTGAAGGGGGTaa                                                                                          >  2:610009/1‑149 (MQ=255)
                                                         aTCCCCTAATACACCGGCGGTTTTTTTCGCCGCCCGTTTTCCCATCACGGAGATATCGTCCAGTAGTGTGGGGATATCATCGAGCAACATCAGTAAAATACTCCCCGCCAAAAACATTTCCTCTCATTTTTATTTTTGAAGGGGGTaa                                                                                          >  2:101378/1‑148 (MQ=255)
                                                         aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTa                                                                                       <  1:615091/151‑1 (MQ=255)
                                                           ccccTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATg                                                                                     <  1:49112/151‑1 (MQ=255)
                                                           ccccTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATg                                                                                     <  1:623942/151‑1 (MQ=255)
                                                                aaTACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGc                                                                                 <  1:138257/150‑1 (MQ=255)
                                                                        ggcggTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                                        >  1:363125/1‑151 (MQ=255)
                                                                           ggTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAg                                                                      <  1:617046/150‑1 (MQ=255)
                                                                           ggTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGc                                                                     <  1:350660/151‑1 (MQ=255)
                                                                                   tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGa                                                                <  1:318303/148‑1 (MQ=255)
                                                                                        gccAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCt                                                        >  2:172850/1‑151 (MQ=255)
                                                                                         ccAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTACGAAGCAAAAACGGCAGCTCTGATACTTGCtt                                                       <  1:630060/151‑1 (MQ=255)
                                                                                            gTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTcac                                                    >  2:237989/1‑151 (MQ=255)
                                                                                              ttGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTcaca                                                   <  1:628938/150‑1 (MQ=255)
                                                                                                gCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGt                                                <  2:281304/151‑1 (MQ=255)
                                                                                                             atatCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAttttttt                                    >  1:31982/1‑150 (MQ=255)
                                                                                                               atCGTCCAGTAGTGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTaa                                  <  1:155653/150‑1 (MQ=255)
                                                                                                                      agtagtGTGGCGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAg                          <  2:165824/151‑1 (MQ=255)
                                                                                                                                cGATATCATCGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATtata                <  1:610026/151‑1 (MQ=255)
                                                                                                                                    atcatcGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtctc            >  1:431120/1‑151 (MQ=255)
                                                                                                                                    atcatcGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtct             <  1:494484/150‑1 (MQ=255)
                                                                                                                                      catcGAGCAACATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATAtctctc          >  2:150055/1‑151 (MQ=255)
                                                                                                                                              aaCATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCcagcag  <  2:447682/151‑1 (MQ=255)
                                                                                                                                              aaCATCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCcagc    >  2:108345/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAG  >  NC_000913/2029193‑2029485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A