Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,449,635	(C)_5→6	coding (415/822 nt)	yfcO ←	DUF2544 family putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,449,630	1	.	C	100.0%	132.0 / NA	38	G140G (GGC→GGG)	yfcO	DUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (15/23); total (15/23)

CAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTG  >  NC_000913/2449494‑2449766

cAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGagag                                                                                                                              <  1:177623/150‑1 (MQ=255)

  aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCt                                                                                                                           >  2:445328/1‑151 (MQ=255)

   ccACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCtt                                                                                                                          <  1:585782/151‑1 (MQ=255)

     acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCa                                                                                                                        <  2:215689/151‑1 (MQ=255)

     acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCa                                                                                                                        <  2:104499/151‑1 (MQ=255)

           cccGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtca                                                                                                                  <  2:509656/151‑1 (MQ=255)

                cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                             <  2:67419/151‑1 (MQ=255)

                  aCTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                                                                           <  2:235550/151‑1 (MQ=255)

                     gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                                                                         <  2:386079/150‑1 (MQ=255)

                            gAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                                                                                  <  1:59749/150‑1 (MQ=255)

                                  ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                            >  1:437011/1‑150 (MQ=255)

                                  ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                            >  1:464731/1‑150 (MQ=255)

                                    ttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACaa                                                                                          <  2:314930/150‑1 (MQ=255)

                                       aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaa                                                                                       >  2:451995/1‑150 (MQ=255)

                                          tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                                                                    >  2:506656/1‑150 (MQ=255)

                                              gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                               <  1:86929/151‑1 (MQ=255)

                                               aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                                                                  >  2:80768/1‑147 (MQ=255)

                                                 aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                            >  2:318576/1‑151 (MQ=255)

                                                  tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                            >  2:30585/1‑150 (MQ=255)

                                                   aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                                                                           <  2:138577/150‑1 (MQ=255)

                                                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                <  2:109776/106‑1 (MQ=255)

                                                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                >  1:109776/1‑106 (MQ=255)

                                                             ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                                                                   >  2:532591/1‑148 (MQ=255)

                                                              cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                                                               <  2:225163/151‑1 (MQ=255)

                                                                 gAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                             <  1:172332/150‑1 (MQ=255)

                                                                              gTTGAACAGAAACGGATGGACAAATCGTGTGGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGACATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAAt                                                >  2:386677/1‑150 (MQ=255)

                                                                                 gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                            <  1:359103/151‑1 (MQ=255)

                                                                                 gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                            <  1:221526/151‑1 (MQ=255)

                                                                                                gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTcc                             <  1:59949/151‑1 (MQ=255)

                                                                                                  cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCAc                           >  1:275529/1‑151 (MQ=255)

                                                                                                        gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGAc                     <  2:479573/151‑1 (MQ=255)

                                                                                                        gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGAc                     <  2:56032/151‑1 (MQ=255)

                                                                                                          gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                   >  1:314737/1‑151 (MQ=255)

                                                                                                            cgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTa                 <  2:86669/151‑1 (MQ=255)

                                                                                                               gACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc              >  2:465952/1‑151 (MQ=255)

                                                                                                                aCTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc             <  1:381404/151‑1 (MQ=255)

                                                                                                                          cccTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACtt   <  1:402140/151‑1 (MQ=255)

                                                                                                                            cTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTg  >  1:354668/1‑150 (MQ=255)

CAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTG  >  NC_000913/2449494‑2449766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A