Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,467,446	A→G	K301R (AAG→AGG)	intS →	CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,467,446	0	A	G	100.0%	153.1 / NA	41	K301R (AAG→AGG)	intS	CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/27); total (14/27)

ACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAAGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTG  >  NC_000913/2467304‑2467588

aCCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCt                                                                                                                                        <  1:474182/151‑1 (MQ=255)

 ccATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTg                                                                                                                                       <  1:353896/151‑1 (MQ=255)

   aTCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCg                                                                                                                                     >  2:32871/1‑151 (MQ=255)

        cGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAAc                                                                                                                                <  2:331393/151‑1 (MQ=255)

        cGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAAc                                                                                                                                <  1:427638/151‑1 (MQ=255)

               gtgATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTAc                                                                                                                         >  2:526578/1‑151 (MQ=255)

                       aGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTtgtg                                                                                                                 <  2:123469/151‑1 (MQ=255)

                         gACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGAt                                                                                                               >  1:253146/1‑151 (MQ=255)

                              aaaaTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATcaaa                                                                                                           <  2:617418/150‑1 (MQ=255)

                                 aTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATcaaacaa                                                                                                        >  2:512921/1‑150 (MQ=255)

                                    cATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATc                                                                                                     <  1:153689/150‑1 (MQ=255)

                                         ggTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTa                                                                                                <  1:513381/150‑1 (MQ=255)

                                         ggTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTa                                                                                                <  2:598991/150‑1 (MQ=255)

                                          gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              <  2:632086/151‑1 (MQ=255)

                                          gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              >  2:334311/1‑151 (MQ=255)

                                            tCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              >  2:352472/1‑149 (MQ=255)

                                                  gTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTg                                                                                      <  1:512020/151‑1 (MQ=255)

                                                       aCCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAg                                                                                 <  2:239779/151‑1 (MQ=255)

                                                               gTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACg                                                                         <  2:78251/151‑1 (MQ=255)

                                                                       tctcACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCa                                                                   <  2:608232/149‑1 (MQ=255)

                                                                       tctcACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCa                                                                   <  2:123094/149‑1 (MQ=255)

                                                                                gCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTc                                                        <  1:362100/151‑1 (MQ=255)

                                                                                      cTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCAc                                                   <  2:223691/150‑1 (MQ=255)

                                                                                       tCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACg                                                  >  1:356490/1‑150 (MQ=255)

                                                                                        ccATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCAAGAt                                                <  2:216034/151‑1 (MQ=255)

                                                                                                     aGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCAc                                   <  2:38493/151‑1 (MQ=255)

                                                                                                      gTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACg                                  >  2:317775/1‑151 (MQ=255)

                                                                                                      gTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACg                                  >  2:347417/1‑151 (MQ=255)

                                                                                                           agagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATgg                             <  1:216535/151‑1 (MQ=255)

                                                                                                           agagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATgg                             <  2:391014/151‑1 (MQ=255)

                                                                                                            gagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGc                            >  1:299425/1‑151 (MQ=255)

                                                                                                                  gtttTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCctgctg                      <  1:437658/151‑1 (MQ=255)

                                                                                                                      ttGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACg                   <  2:464607/150‑1 (MQ=255)

                                                                                                                        gCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCt                 >  1:533421/1‑150 (MQ=255)

                                                                                                                          cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  2:512414/151‑1 (MQ=255)

                                                                                                                          cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  1:347424/151‑1 (MQ=255)

                                                                                                                          cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  1:381119/151‑1 (MQ=255)

                                                                                                                              cgcAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGa            >  1:398323/1‑149 (MQ=255)

                                                                                                                              cgcAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGa            >  1:615380/1‑149 (MQ=255)

                                                                                                                                  aCGATAAGAAGAGGCCAATCTGTGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCa      <  1:210429/151‑1 (MQ=255)

                                                                                                                                      tAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTg  >  1:112823/1‑151 (MQ=255)

ACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAAGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTG  >  NC_000913/2467304‑2467588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A