Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,982	C→T	P97S (CCG→TCG)	entB →	isochorismatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,982	0	C	T	100.0%	175.9 / NA	45	P97S (CCG→TCG)	entB	isochorismatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/20); total (25/20)

TGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAG  >  NC_000913/627841‑628128

tGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCAGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTg                                                                                                                                            >  1:99590/1‑150 (MQ=255)

         atgGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGATCGc                                                                                                                                  <  1:18598/151‑1 (MQ=255)

             aGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGa                                                                                                                              <  1:388404/151‑1 (MQ=255)

              gCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGaa                                                                                                                             <  2:246666/151‑1 (MQ=255)

                     atagcgAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACACTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACa                                                                                                                           >  2:750070/4‑146 (MQ=255)

                     aTCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCaaa                                                                                                                      <  1:542101/151‑1 (MQ=255)

                       cgcgAATATTGCTGCACTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCaaa                                                                                                                      >  2:1045336/1‑149 (MQ=255)

                              atTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGgtggtg                                                                                                              <  2:149837/150‑1 (MQ=255)

                                   tgcgctgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGc                                                                                                        >  2:454707/1‑151 (MQ=255)

                                       ctgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTg                                                                                                     >  2:676445/1‑150 (MQ=255)

                                        tgcgcGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGAc                                                                                                   >  1:531642/1‑151 (MQ=255)

                                               ccactgataaCAGCATAATATCCCGGCTTATCACACCGCTCAGCCGACGGTGCAGAGCGACGAAGAATGGGCCCTGTTGGATGTTATGTGTGGGTCTCTCCTAACCCGGCCGCCGGATTCGCAATTTGTGGTGGATCGCCTGACGcc                                                                                                >  2:683347/9‑147 (MQ=255)

                                                tactGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATg                                                                                           >  1:3581/1‑151 (MQ=255)

                                                tactGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATg                                                                                           >  2:436697/1‑151 (MQ=255)

                                                  ctGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGcc                                                                                         >  2:519595/1‑151 (MQ=255)

                                                         cAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGaca                                                                                  <  1:76413/151‑1 (MQ=255)

                                                           gCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACg                                                                                >  1:63417/1‑151 (MQ=255)

                                                               aaTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGc                                                                            <  1:272316/151‑1 (MQ=255)

                                                               aaTATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGc                                                                            >  2:143974/1‑151 (MQ=255)

                                                                  atCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTg                                                                          <  1:660408/150‑1 (MQ=255)

                                                                    cccGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTg                                                                       <  1:136259/151‑1 (MQ=255)

                                                                      cGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGa                                                                      >  1:970344/1‑150 (MQ=255)

                                                                      cGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGa                                                                      >  2:1067457/1‑150 (MQ=255)

                                                                         tttATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGCGCTGGTGAAGTg                                                                  >  2:855626/1‑151 (MQ=255)

                                                                                cacCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTAc                                                           >  1:189649/1‑151 (MQ=255)

                                                                                 acCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGACTGACGTCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACa                                                          <  1:131434/151‑1 (MQ=255)

                                                                                  ccGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTAc                                                           >  2:1011367/1‑149 (MQ=255)

                                                                                     cccAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAgcgc                                                      >  2:762875/1‑151 (MQ=255)

                                                                                           cGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTc                                                 >  1:439498/1‑150 (MQ=255)

                                                                                                  gcagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtct                                         <  1:215502/151‑1 (MQ=255)

                                                                                                   cagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtctc                                        >  1:668719/1‑151 (MQ=255)

                                                                                                   cagagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTtctc                                        <  1:605951/151‑1 (MQ=255)

                                                                                                    agagcGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTTTcc                                       >  1:779861/1‑151 (MQ=255)

                                                                                                          aTGAAGATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTgg                                  >  1:513237/1‑150 (MQ=255)

                                                                                                               gATCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCaaa                            <  1:990659/151‑1 (MQ=255)

                                                                                                                aTCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAAt                           <  1:167969/151‑1 (MQ=255)

                                                                                                                 tCGGGCGCTGTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATg                          >  2:215861/1‑151 (MQ=255)

                                                                                                                          gTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAag                   <  1:182464/149‑1 (MQ=255)

                                                                                                                          gTTGAATGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAag                   <  1:155796/149‑1 (MQ=255)

                                                                                                                              aaTGATATGTGGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTg             <  2:941067/151‑1 (MQ=255)

                                                                                                                                     tgtgGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTa       >  1:616379/1‑150 (MQ=255)

                                                                                                                                       tgGGGGTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAAcc    >  1:274806/1‑151 (MQ=255)

                                                                                                                                        gggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAAcc    <  1:535089/150‑1 (MQ=255)

                                                                                                                                        gggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCa   <  2:181527/151‑1 (MQ=255)

                                                                                                                                         ggggTCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAg  <  1:379376/151‑1 (MQ=255)

TGCCCGATGATGGAGCAGGTGATCGCGAATATTGCTGCGCTGCGCGACTACTGCAAACAGCACAATATCCCGGTTTATTACACCGCCCAGCCGAAAGAGCAGAGCGATGAAGATCGGGCGCTGTTGAATGATATGTGGGGGCCGGGCCTGACCCGCTCGCCGGAACAGCAAAAGGTGGTGGATCGCCTGACGCCAGATGCCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTCCGCTGGAGCAAATGCTGAAAGAGAGTGGACGTAACCAG  >  NC_000913/627841‑628128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A