Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	628,546	A→G	K285E (AAA→GAA)	entB →	isochorismatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	628,546	0	A	G	100.0%	151.9 / NA	41	K285E (AAA→GAA)	entB	isochorismatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/22); total (19/22)

CGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCG  >  NC_000913/628410‑628691

cGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCa                                                                                                                                      <  1:36563/151‑1 (MQ=255)

 gACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTc                                                                                                                                       >  1:330496/1‑149 (MQ=255)

           tGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           >  2:99471/1‑151 (MQ=255)

             gATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTAATGCTGGCTAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGt                                                                                                                         <  2:174137/151‑1 (MQ=255)

                 cGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTgg                                                                                                                     <  2:586649/151‑1 (MQ=255)

                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTaa                                                                                                                 <  1:36526/151‑1 (MQ=255)

                      cgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           >  1:567924/1‑140 (MQ=255)

                      cgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           <  2:567912/140‑1 (MQ=255)

                               gcgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                                                                                       <  1:356094/151‑1 (MQ=255)

                               gcgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                                                                                       >  2:307686/1‑151 (MQ=255)

                                    ggcggcGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGGGGTAAAAATGTCTGGATAACCGGCGCAGGTaaa                                                                                                   <  2:565429/150‑1 (MQ=255)

                                      cggcgCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGt                                                                                                >  2:587991/1‑151 (MQ=255)

                                        gcgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                                                                                              <  1:160375/151‑1 (MQ=255)

                                        gcgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                                                                                              >  1:547230/1‑151 (MQ=255)

                                             cTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCt                                                                                         >  2:160332/1‑151 (MQ=255)

                                                    aaaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCAc                                                                                  >  1:472112/1‑151 (MQ=255)

                                                      aGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACgg                                                                                <  1:146994/151‑1 (MQ=255)

                                                       gTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgc                                                                               <  1:160337/151‑1 (MQ=255)

                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTg                                                                           <  1:449653/151‑1 (MQ=255)

                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTg                                                                           >  2:318229/1‑151 (MQ=255)

                                                                gATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGtt                                                                      >  1:2527/1‑151 (MQ=255)

                                                                   atCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttg                                                                    <  1:288607/150‑1 (MQ=255)

                                                                                 gCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑g                                                     <  1:479738/150‑1 (MQ=255)

                                                                                 gCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Ga                                                    <  1:270496/151‑1 (MQ=255)

                                                                                   tGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Gaa                                                   >  2:473347/1‑150 (MQ=255)

                                                                                   tGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Gaa                                                   >  1:103174/1‑150 (MQ=255)

                                                                                    ggCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACTGCGCTGGTGTTTTTTGAGGCGGGAGC‑Gaaa                                                  <  2:100151/150‑1 (MQ=255)

                                                                                        aaaaaCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGGAAAGta                                               >  1:611686/1‑149 (MQ=255)

                                                                                           aaCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAgg                                          >  2:389176/1‑151 (MQ=255)

                                                                                            aCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTACAgg                                          >  2:541631/1‑150 (MQ=255)

                                                                                            aCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGATACCgg                                          >  2:365341/1‑150 (MQ=255)

                                                                                             cccGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGGGC‑GAAAGGTACAGGtt                                        >  2:519122/1‑151 (MQ=255)

                                                                                             cccGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGt                                         <  1:594736/150‑1 (MQ=255)

                                                                                                    aTCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTAAAGGGTTTGATc                                  >  1:371336/1‑150 (MQ=255)

                                                                                                        aCGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCg                             <  1:414504/151‑1 (MQ=255)

                                                                                                        aCGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCg                             <  2:84859/151‑1 (MQ=255)

                                                                                                               gtggAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTACAGGGTTTGATCAAGCGGTCACTc                      >  1:191150/1‑151 (MQ=255)

                                                                                                                   aaGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGa                  <  2:543567/151‑1 (MQ=255)

                                                                                                                             cccGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAatat           <  2:459756/148‑1 (MQ=255)

                                                                                                                                  gAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGc   <  1:403004/151‑1 (MQ=255)

                                                                                                                                   aGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCg  <  1:242558/151‑1 (MQ=255)

CGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCG  >  NC_000913/628410‑628691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A