Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,219	A→G	V496V (GTT→GTC)	uvrC ←	excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,219	0	A	G	100.0%	173.3 / NA	50	V496V (GTT→GTC)	uvrC	excinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/28); total (22/28)

CCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATAA  >  NC_000913/1993075‑1993360

cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTGCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCa                                                                                                                                         <  2:228977/151‑1 (MQ=255)

 ccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCaa                                                                                                                                        <  1:514313/151‑1 (MQ=255)

  cGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAg                                                                                                                                       <  1:969288/151‑1 (MQ=255)

    ccAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGt                                                                                                                                      >  1:801023/1‑150 (MQ=255)

     cAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGt                                                                                                                                      >  1:427927/1‑149 (MQ=255)

      aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagc                                                                                                                                   >  2:593492/1‑151 (MQ=255)

       aTCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagc                                                                                                                                   >  1:1047243/1‑150 (MQ=255)

         cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagcagc                                                                                                                                >  2:588586/1‑151 (MQ=255)

         cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagcag                                                                                                                                 <  1:709128/150‑1 (MQ=255)

            gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCgg                                                                                                                              >  1:923336/1‑150 (MQ=255)

              gATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgat                                                                                                                            <  2:141552/150‑1 (MQ=255)

              gATCATGTGATTCATCGCGAATATGCTGGATAACATGCAACGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgatg                                                                                                                           <  1:316631/151‑1 (MQ=255)

                tCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgatgat                                                                                                                         >  2:243589/1‑151 (MQ=255)

                         tCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATccc                                                                                                                >  2:228064/1‑151 (MQ=255)

                            tCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATg                                                                                                             >  2:499866/1‑151 (MQ=255)

                             cgcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATga                                                                                                            <  1:819186/151‑1 (MQ=255)

                             cgcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATg                                                                                                             <  2:644569/150‑1 (MQ=255)

                                   tatGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATc                                                                                                      >  2:1033833/1‑151 (MQ=255)

                                    atGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATcc                                                                                                     <  2:427901/151‑1 (MQ=255)

                                    atGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTCCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATc                                                                                                      <  2:185645/150‑1 (MQ=255)

                                      gCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATcc                                                                                                     >  1:327923/1‑149 (MQ=255)

                                         ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGtt                                                                                                 <  1:793846/150‑1 (MQ=255)

                                         ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGtt                                                                                                 <  2:1021405/150‑1 (MQ=255)

                                         ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTc                                                                                                <  2:292462/151‑1 (MQ=255)

                                             aaCATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGc                                                                                             <  2:540498/150‑1 (MQ=255)

                                                aTGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAg                                                                                         <  2:550228/151‑1 (MQ=255)

                                                   cAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGAc                                                                                       >  2:409091/1‑150 (MQ=255)

                                                     gcgcGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTCCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGTCATCCAGTTCGGCGAAGCCAtt                                                                                    >  2:85961/1‑151 (MQ=255)

                                                      cgcgGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAttt                                                                                   <  1:801045/151‑1 (MQ=255)

                                                        cgGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAttttt                                                                                 >  2:972455/1‑151 (MQ=255)

                                                          ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCg                                                                               <  1:151950/151‑1 (MQ=255)

                                                           gTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGc                                                                              >  1:345045/1‑151 (MQ=255)

                                                                       gcAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTg                                                                  <  1:105602/151‑1 (MQ=255)

                                                                            cTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTCTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCtt                                                             >  2:230949/1‑151 (MQ=255)

                                                                                   ccTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTgccgcc                                                      <  1:77605/151‑1 (MQ=255)

                                                                                    cTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGccgccg                                                     >  1:875172/1‑151 (MQ=255)

                                                                                          ttCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGAt                                                >  1:746501/1‑150 (MQ=255)

                                                                                               gCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGAt                                          <  1:571744/151‑1 (MQ=255)

                                                                                                   acaGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCCCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGCTcac                                       >  2:954195/3‑150 (MQ=255)

                                                                                                     aGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATc                                    <  1:650683/151‑1 (MQ=255)

                                                                                                       aaCAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATcc                                   >  1:398527/1‑150 (MQ=255)

                                                                                                         cAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCggg                                >  2:662578/1‑151 (MQ=255)

                                                                                                             gTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATc                             <  2:271728/150‑1 (MQ=255)

                                                                                                               ttCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCtt                           <  1:453720/150‑1 (MQ=255)

                                                                                                                   agtccagCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt                      <  2:820564/151‑1 (MQ=255)

                                                                                                                      ccagCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgtcg                    <  1:826540/150‑1 (MQ=255)

                                                                                                                                gATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCttt         >  2:510799/1‑151 (MQ=255)

                                                                                                                                     gCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTa        <  2:667943/147‑1 (MQ=255)

                                                                                                                                     gCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTAc       <  1:721251/148‑1 (MQ=255)

                                                                                                                                        ccTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATaa  <  1:5406/150‑1 (MQ=255)

CCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATAA  >  NC_000913/1993075‑1993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A