Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,605	G→A	intergenic (+84/‑63)	yegP → / → yegQ	UPF0339 family protein/putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,605	0	G	A	100.0%	192.6 / NA	51	intergenic (+84/‑63)	yegP/yegQ	UPF0339 family protein/putative peptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/33); total (18/33)

GGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATG  >  NC_000913/2165465‑2165752

ggAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCt                                                                                                                                           >  1:254732/1‑151 (MQ=255)

  aaTTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTa                                                                                                                                          >  2:508865/1‑150 (MQ=255)

    ttGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAAcc                                                                                                                                       >  2:448485/1‑151 (MQ=255)

             ttAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTtcatc                                                                                                                               >  1:477949/1‑150 (MQ=255)

                  gCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTg                                                                                                                          >  2:548853/1‑150 (MQ=255)

                   ccAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGaa                                                                                                                        <  1:739475/151‑1 (MQ=255)

                        ggCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAGGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                                                                                   <  2:915348/151‑1 (MQ=255)

                            caAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                <  2:566122/150‑1 (MQ=255)

                                 cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTg                                                                                                          <  1:382638/151‑1 (MQ=255)

                                       gTGAAAGCCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatca                                                                                                    <  2:214678/151‑1 (MQ=255)

                                       gTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatc                                                                                                     >  1:726509/1‑150 (MQ=255)

                                         gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg                                                                                                   <  1:757405/150‑1 (MQ=255)

                                           aaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAAc                                                                                                >  2:260542/1‑151 (MQ=255)

                                            aGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg                                                                                               <  2:438661/151‑1 (MQ=255)

                                              aCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgag                                                                                             <  1:853989/151‑1 (MQ=255)

                                               cAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgaga                                                                                            <  1:515574/151‑1 (MQ=255)

                                                  tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagaga                                                                                          >  1:368571/1‑150 (MQ=255)

                                                   aCGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa                                                                                         <  2:1026690/150‑1 (MQ=255)

                                                      tAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTAt                                                                                     >  1:824739/1‑151 (MQ=255)

                                                                 ccGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGACGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCgg                                                                          >  2:39914/1‑151 (MQ=255)

                                                                       gTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTcc                                                                    <  1:775604/151‑1 (MQ=255)

                                                                         gggTTGTTGGCGTCCGGCGCTTTACCATAACCCTCCACAGCGCTACAATGCACGCCCTTAAAGTGGGAGCACTCCACTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTGAAACCGGAACTCCt                                                                   <  2:680958/150‑1 (MQ=255)

                                                                         gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCtt                                                                  <  1:690651/151‑1 (MQ=255)

                                                                         gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCtt                                                                  <  1:824464/151‑1 (MQ=255)

                                                                           gttgttGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTc                                                                <  2:957000/151‑1 (MQ=255)

                                                                              gttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTcccc                                                             <  2:254707/151‑1 (MQ=255)

                                                                                tgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcg                                                            <  1:4908/150‑1 (MQ=255)

                                                                                tgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcg                                                            <  2:1000128/150‑1 (MQ=255)

                                                                                   cGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcggcgg                                                        <  2:967093/151‑1 (MQ=255)

                                                                                   cGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcggcg                                                         <  1:93324/150‑1 (MQ=255)

                                                                                       cGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAAc                                                    <  2:373903/151‑1 (MQ=255)

                                                                                        ggCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACg                                                   <  2:335909/151‑1 (MQ=255)

                                                                                           gcTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTg                                                >  1:480496/1‑151 (MQ=255)

                                                                                           gcTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTg                                                <  1:685115/151‑1 (MQ=255)

                                                                                              ttACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGaa                                              >  1:767005/1‑150 (MQ=255)

                                                                                               tACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGaaa                                             >  1:928188/1‑150 (MQ=255)

                                                                                                 ccAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          >  1:998263/1‑151 (MQ=255)

                                                                                                 ccAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          <  1:490936/151‑1 (MQ=255)

                                                                                                  cAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          >  1:499530/1‑150 (MQ=255)

                                                                                                   agagCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAatat                                        <  1:301855/151‑1 (MQ=255)

                                                                                                      gCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCg                                     >  2:786429/1‑151 (MQ=255)

                                                                                                          cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTAc                                 <  2:155940/151‑1 (MQ=255)

                                                                                                          cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTAc                                 <  1:47316/151‑1 (MQ=255)

                                                                                                                gcgcTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                           <  2:619774/151‑1 (MQ=255)

                                                                                                                     aCAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCtt                       <  2:1014518/150‑1 (MQ=255)

                                                                                                                     aCAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCt                        <  2:726755/149‑1 (MQ=255)

                                                                                                                       aaTGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATg                    <  2:667693/151‑1 (MQ=255)

                                                                                                                        aTGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATgg                   >  1:707023/1‑151 (MQ=255)

                                                                                                                             cgcccTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGgcgc                >  1:325715/1‑149 (MQ=255)

                                                                                                                                   tAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGc          <  1:309648/149‑1 (MQ=255)

                                                                                                                                       gTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTa    <  1:740475/151‑1 (MQ=255)

                                                                                                                                         gggagCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATg  >  1:912408/1‑151 (MQ=255)

GGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATG  >  NC_000913/2165465‑2165752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A