Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,328,783	(G)_5→6	coding (1015/1650 nt)	yfaQ ←	tandem DUF2300 domain protein, putative host defense protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,328,778	1	.	G	97.0%	235.6 / ‑1.1	66	coding (1020/1650 nt)	yfaQ	tandem DUF2300 domain protein, putative host defense protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (35/29); total (35/31)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑GG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328635‑2328923

aTCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCg‑gg‑ggggc                                                                                                                                             >  2:742808/1‑149 (MQ=255)

 tCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑gg‑ggggcg                                                                                                                                            >  1:585552/1‑148 (MQ=255)

  cGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑gg‑ggggcgt                                                                                                                                           >  1:16940/1‑147 (MQ=255)

  cGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑gg‑ggggcgt                                                                                                                                           >  1:505712/1‑147 (MQ=255)

        cgcCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACtttt                                                                                                                                     >  2:285190/1‑151 (MQ=255)

         gcCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTc                                                                                                                                    <  1:1027645/151‑1 (MQ=255)

         aaccccGGGGTAGATGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCACGAATGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACtttt                                                                                                                                     >  2:521968/3‑150 (MQ=255)

               gTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTcg                                                                                                                              >  2:462339/1‑151 (MQ=255)

                 ggTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTcgcg                                                                                                                            <  2:810862/151‑1 (MQ=255)

                 ggTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTcgcg                                                                                                                            >  1:429657/1‑151 (MQ=255)

                   tCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGAt                                                                                                                          >  1:237700/1‑151 (MQ=255)

                     gCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATcc                                                                                                                        >  2:26152/1‑151 (MQ=255)

                        ggCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑GGTGGG‑CGTACTTTTCGCCTCGCGATCCAg                                                                                                                      <  2:820436/150‑1 (MQ=255)

                          cGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAAc                                                                                                                   <  2:156565/151‑1 (MQ=255)

                           gAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAAct                                                                                                                  >  1:615604/1‑151 (MQ=255)

                            aaGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAActc                                                                                                                 >  1:893422/1‑151 (MQ=255)

                             aGCAGAAACACGCTGCGTGTCGCTGCTGTCGGGGATGGTCAAGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTAGCCTCGCGATCCAGAtctcg                                                                                                                <  2:808462/151‑2 (MQ=255)

                             aGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAActct                                                                                                                <  1:604637/151‑1 (MQ=255)

                              gCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTg                                                                                                               <  2:356595/151‑1 (MQ=255)

                              gCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGAAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTg                                                                                                               >  1:480362/1‑151 (MQ=255)

                                     cacGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGt                                                                                                        >  2:645819/1‑151 (MQ=255)

                                      acGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGt                                                                                                        >  2:852042/1‑150 (MQ=255)

                                         cTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTActc                                                                                                    >  1:341446/1‑151 (MQ=255)

                                          tGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTActct                                                                                                   >  1:542410/1‑151 (MQ=255)

                                              tGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCAc                                                                                               >  2:905549/1‑151 (MQ=255)

                                                gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                                                                                             <  1:491723/151‑1 (MQ=255)

                                                gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGa                                                                                             <  2:588215/151‑1 (MQ=255)

                                                        gTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGAt                                                                                      <  1:440750/150‑1 (MQ=255)

                                                                tGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGc                                                                              >  1:381057/1‑150 (MQ=255)

                                                                         aaTCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGtt                                                                    >  2:836841/1‑151 (MQ=255)

                                                                            cGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTg                                                                  <  2:832173/150‑1 (MQ=255)

                                                                            cGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTg                                                                  <  2:117048/150‑1 (MQ=255)

                                                                             gCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGcc                                                                >  1:659917/1‑151 (MQ=255)

                                                                                 tCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCt                                                              <  1:267802/149‑1 (MQ=255)

                                                                                  cccGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCttt                                                            <  1:312327/150‑1 (MQ=255)

                                                                                    cGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCg                                                         >  1:124032/1‑151 (MQ=255)

                                                                                      aTTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCt                                                       >  1:364280/1‑151 (MQ=255)

                                                                                      aTTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCt                                                       >  1:358561/1‑151 (MQ=255)

                                                                                        tGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTc                                                      >  1:804495/1‑150 (MQ=255)

                                                                                         ggCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTc                                                      >  1:846715/1‑149 (MQ=255)

                                                                                         ggCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCa                                                     <  2:346999/150‑1 (MQ=255)

                                                                                           cGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGt                                                   >  1:31948/1‑150 (MQ=255)

                                                                                               ttGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              >  2:38925/1‑151 (MQ=255)

                                                                                               ttGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              >  1:1000508/1‑151 (MQ=255)

                                                                                                tGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑GG‑GGGACGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgt                                              <  2:313513/150‑1 (MQ=255)

                                                                                                 gCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGgtcg                                            <  2:804449/151‑1 (MQ=255)

                                                                                                    gTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGATCAGTAAGgtcgtc                                          >  1:1020290/1‑150 (MQ=255)

                                                                                                      aaaaaaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCa                                       <  1:721180/151‑1 (MQ=255)

                                                                                                          aaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGt                                   >  1:781341/1‑151 (MQ=255)

                                                                                                          aaGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGCCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGt                                   >  2:182055/1‑151 (MQ=255)

                                                                                                           aGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACCGCAATTTCAGTTTGCCCTTTTAGCTCAGTACGGTCGTCTCACCGTg                                  >  2:835423/1‑151 (MQ=255)

                                                                                                            gTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTgg                                 <  1:271712/151‑1 (MQ=255)

                                                                                                               cGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              <  1:556293/151‑1 (MQ=255)

                                                                                                                gAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              >  1:1040370/1‑150 (MQ=255)

                                                                                                                gAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTg                              >  2:172238/1‑150 (MQ=255)

                                                                                                                  aTCGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGGCTCACCGTGGCTg                              <  2:198846/148‑1 (MQ=255)

                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTTCTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCACTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaaa                         <  2:676832/151‑1 (MQ=255)

                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAGCGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaa                          <  2:911118/150‑1 (MQ=255)

                                                                                                                    cGCCACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaa                          >  1:947831/1‑150 (MQ=255)

                                                                                                                      ccACGGTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACaaa                         >  2:374168/1‑149 (MQ=255)

                                                                                                                           gTCATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtg                  <  1:521997/151‑1 (MQ=255)

                                                                                                                             cATGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtgtg                <  1:171559/151‑1 (MQ=255)

                                                                                                                              aTGGCTTTCGCTGCCTCAGGG‑GGG‑CGTGCTTTTCGCCTCGCGATCCAGAACTCTTGCAACGTATTCTTCACGATCGAGATGCGACTGCAACTTCAGTTTGCCCTTTTCGCGCAGTAAGGTCGTCTCACCGTGGCTGACAAAc                        <  2:177324/142‑1 (MQ=255)

                                                                                                                              aTGGCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTgg               <  2:386213/151‑1 (MQ=255)

                                                                                                                                ggCTTTCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGtt             <  2:822249/151‑1 (MQ=255)

                                                                                                                                    ttCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTAcc          >  2:639468/1‑150 (MQ=255)

                                                                                                                                    ttCGCTGCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTAcc          <  1:420378/150‑1 (MQ=255)

                                                                                                                                          gCCTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCGCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa   <  2:555340/151‑1 (MQ=255)

                                                                                                                                           ccTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaa  <  2:234620/151‑1 (MQ=255)

                                                                                                                                           ccTCAGGG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa   >  2:138494/1‑150 (MQ=255)

ATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCA‑GG‑GGG‑CGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAA  >  NC_000913/2328635‑2328923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A