Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I2 R1 139 64.5 2139856 92.7% 1983646 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,337,019 G→A A801V (GCC→GTC)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,337,0190GA100.0% 219.0 / NA 56A801V (GCC→GTC) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/30);  total (26/30)

ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG  >  NC_000913/2336874‑2337165
                                                                                                                                                 |                                                                                                                                                  
aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATc                                                                                                                                                <  2:936061/150‑1 (MQ=255)
aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCg                                                                                                                                               >  2:798050/1‑151 (MQ=255)
    aGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTg                                                                                                                                            >  2:396749/1‑150 (MQ=255)
       tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGatc                                                                                                                                         >  1:953360/1‑150 (MQ=255)
          tCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGatcatc                                                                                                                                      <  2:820208/150‑1 (MQ=255)
           ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGAtcatca                                                                                                                                     <  1:870018/150‑1 (MQ=255)
           ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATcatcat                                                                                                                                    <  1:926021/151‑1 (MQ=255)
              cGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGAt                                                                                                                                 <  1:282053/151‑1 (MQ=255)
              cGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGAt                                                                                                                                 <  1:943801/151‑1 (MQ=255)
               gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGGTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATc                                                                                                                                <  2:984850/151‑1 (MQ=255)
                 cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCt                                                                                                                               <  2:445279/150‑1 (MQ=255)
                   aCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGt                                                                                                                            >  1:488559/1‑151 (MQ=255)
                   aCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGt                                                                                                                            <  1:896282/151‑1 (MQ=255)
                              aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                 >  1:994322/1‑151 (MQ=255)
                              aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                 <  1:954063/151‑1 (MQ=255)
                                      gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAc                                                                                                         >  1:660351/1‑151 (MQ=255)
                                        aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcg                                                                                                       <  1:505328/151‑1 (MQ=255)
                                         gACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcgc                                                                                                      >  1:935164/1‑151 (MQ=255)
                                          aCCCACTACGTTTTCAACTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcg                                                                                                       <  2:745273/149‑1 (MQ=255)
                                            ccACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                                                                                                   >  1:933831/1‑151 (MQ=255)
                                             cACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                  <  2:663091/151‑1 (MQ=255)
                                                tACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                >  2:492271/1‑150 (MQ=255)
                                                 aCGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaaca                                                                                               <  1:809460/150‑1 (MQ=255)
                                                  cGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                <  1:64101/148‑1 (MQ=255)
                                                     tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTa                                                                                           >  1:473264/1‑150 (MQ=255)
                                                       tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAc                                                                                        >  1:39359/1‑151 (MQ=255)
                                                       tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAc                                                                                        >  2:129843/1‑151 (MQ=255)
                                                        cATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                       >  2:298865/1‑151 (MQ=255)
                                                           cTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGtt                                                                                    >  2:442484/1‑151 (MQ=255)
                                                                 cAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                              >  1:1061389/1‑151 (MQ=255)
                                                                 cAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                              <  1:1051787/151‑1 (MQ=255)
                                                                   gTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCgg                                                                            <  1:212243/151‑1 (MQ=255)
                                                                     aCGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTa                                                                          <  1:545281/151‑1 (MQ=255)
                                                                        gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                       >  1:631478/1‑151 (MQ=255)
                                                                           gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTg                                                                    >  2:926435/1‑151 (MQ=255)
                                                                            aGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGa                                                                   <  2:777784/151‑1 (MQ=255)
                                                                             ggATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                  <  1:442510/151‑1 (MQ=255)
                                                                              gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATg                                                                 >  2:467311/1‑151 (MQ=255)
                                                                              gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATg                                                                 >  2:562780/1‑151 (MQ=255)
                                                                                tCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATgg                                                                >  1:165077/1‑150 (MQ=255)
                                                                                 cACGCCCTGGGTGTTACGGCCCCCGATGCTAATTTCCAAAACGCGAGTCCGTCCCAGCTTACCGACATCGGTGTTCATAATGATCTGGTCGCAGCCATCTCCCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACTTTGATgg                                                                <  2:324762/149‑1 (MQ=255)
                                                                                 cACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgag                                                              <  1:18774/151‑1 (MQ=255)
                                                                                    gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                            >  1:484647/1‑150 (MQ=255)
                                                                                         gggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTTCGTTCGGTAACCTTGATGGAGATAAcccc                                                      <  1:440556/151‑1 (MQ=255)
                                                                                          ggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                     <  1:629085/151‑1 (MQ=255)
                                                                                              ttACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                 >  1:830705/1‑151 (MQ=255)
                                                                                                    cccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                           >  1:911183/1‑151 (MQ=255)
                                                                                                     ccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                          <  1:436621/151‑1 (MQ=255)
                                                                                                     ccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                          >  2:989119/1‑151 (MQ=255)
                                                                                                      cccGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATTGAGATAACCCCTTTCGTCGCAcgc                                         <  2:1069375/149‑1 (MQ=255)
                                                                                                       aCGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                        <  1:989171/151‑1 (MQ=255)
                                                                                                                   tCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTggg                             <  1:217780/150‑1 (MQ=255)
                                                                                                                       aaaCGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                        <  1:548887/151‑1 (MQ=255)
                                                                                                                                    tacCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGt            >  1:432985/1‑150 (MQ=255)
                                                                                                                                      ccAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg         <  1:115742/151‑1 (MQ=255)
                                                                                                                                             aCCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg  >  2:846474/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG  >  NC_000913/2336874‑2337165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A