Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,548,346	G→A	G82D (GGC→GAC)	yfeW →	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,548,346	0	G	A	100.0%	229.9 / NA	60	G82D (GGC→GAC)	yfeW	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/26); total (34/26)

ACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTG  >  NC_000913/2548208‑2548486

ccGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCACGTTGCTGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGa                                                                                                                                   >  2:339116/2‑150 (MQ=255)

      caCGGCTTAAGCAGATGGTTCGCTGGATTAGCCAGCAAGTTGCTGTCGGTTATCCCAGCGGAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGa                                                                                                                               >  2:237169/2‑148 (MQ=255)

         ggCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGcc                                                                                                                         >  2:418485/1‑151 (MQ=255)

              aaCCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGTGTGTTGATGGAACAGCCTGTCa                                                                                                                    <  2:825777/151‑1 (MQ=255)

               aCCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGGACGATGACAGCGTGTTGATGGAACAGCCTGTCa                                                                                                                    >  2:298218/1‑150 (MQ=255)

                ccAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCaa                                                                                                                   >  1:830982/1‑150 (MQ=255)

                     tGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGccac                                                                                                             >  2:638320/1‑151 (MQ=255)

                          cGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCg                                                                                                        >  1:852896/1‑151 (MQ=255)

                              gtaTTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGa                                                                                                     <  2:627649/148‑1 (MQ=255)

                                    gCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTa                                                                                              <  1:286542/151‑1 (MQ=255)

                                      cAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  2:522539/1‑151 (MQ=255)

                                       aGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  1:610678/1‑150 (MQ=255)

                                       aGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  2:214977/1‑150 (MQ=255)

                                           aGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTg                                                                                       <  2:290333/151‑1 (MQ=255)

                                                 tGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTc                                                                                  >  1:278049/1‑150 (MQ=255)

                                                    cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCaaa                                                                               <  2:46751/150‑1 (MQ=255)

                                                    cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAac                                                                              >  2:1015539/1‑151 (MQ=255)

                                                               gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGt                                                                    >  1:616189/1‑150 (MQ=255)

                                                               gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTa                                                                   <  1:768303/151‑1 (MQ=255)

                                                                cGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTa                                                                   <  2:123108/150‑1 (MQ=255)

                                                                 gTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTAc                                                                  <  2:407678/150‑1 (MQ=255)

                                                                  tAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGc                                                                >  1:883174/1‑151 (MQ=255)

                                                                    aaCCTGCTGATCATTAAAGATAATCAGATGGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCa                                                              <  2:402697/151‑1 (MQ=255)

                                                                         gctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGa                                                           <  2:152518/149‑1 (MQ=255)

                                                                          ctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACt                                                        >  2:113467/1‑151 (MQ=255)

                                                                            gATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTc                                                      <  2:116701/151‑1 (MQ=255)

                                                                               cATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGc                                                    <  1:660426/150‑1 (MQ=255)

                                                                                  tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTg                                                >  2:483027/1‑151 (MQ=255)

                                                                                  tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACATGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTg                                                >  2:452004/1‑151 (MQ=255)

                                                                                        tAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaa                                            >  2:861436/1‑149 (MQ=255)

                                                                                        tAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGGGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaa                                            >  1:107628/1‑149 (MQ=255)

                                                                                          aTCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCt                                        >  2:90932/1‑151 (MQ=255)

                                                                                           tCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtg                                       >  1:184254/1‑151 (MQ=255)

                                                                                            cAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtg                                       <  2:883127/150‑1 (MQ=255)

                                                                                             aGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgat                                     >  1:786485/1‑151 (MQ=255)

                                                                                              gATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatg                                    >  1:416176/1‑151 (MQ=255)

                                                                                                  gtgtATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGc                                 <  2:587662/150‑1 (MQ=255)

                                                                                                  gtgtATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCg                                <  1:858902/151‑1 (MQ=255)

                                                                                                     tATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGa                               >  2:1026140/1‑149 (MQ=255)

                                                                                                      aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  2:15311/1‑151 (MQ=255)

                                                                                                      aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            <  1:215002/151‑1 (MQ=255)

                                                                                                      aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  1:488881/1‑151 (MQ=255)

                                                                                                        cGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  2:520437/1‑149 (MQ=255)

                                                                                                        cGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGc                           >  1:608944/1‑150 (MQ=255)

                                                                                                         gCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCaa                         <  1:289614/151‑1 (MQ=255)

                                                                                                           aaaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAAc                       >  2:248023/1‑151 (MQ=255)

                                                                                                            aaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACt                      >  1:794217/1‑151 (MQ=255)

                                                                                                               ccTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCa                   <  2:479163/151‑1 (MQ=255)

                                                                                                                cTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCAt                  >  2:1059648/1‑151 (MQ=255)

                                                                                                                  ggggAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATc                 <  1:33562/150‑1 (MQ=255)

                                                                                                                    ggAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTg              >  1:184959/1‑151 (MQ=255)

                                                                                                                       gcggcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACg           <  1:519031/151‑1 (MQ=255)

                                                                                                                        cggcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGa          <  2:1004439/151‑1 (MQ=255)

                                                                                                                          gcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATc        >  2:986534/1‑151 (MQ=255)

                                                                                                                           cgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCg       >  2:70267/1‑151 (MQ=255)

                                                                                                                           cgAAAAAGTACGATGACAGCGTGTTGATGCAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATc        <  2:525273/150‑1 (MQ=255)

                                                                                                                             aaaaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGa     <  2:379879/151‑1 (MQ=255)

                                                                                                                               aaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGAtt   <  1:662610/151‑1 (MQ=255)

                                                                                                                               aaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGAtt   <  2:1062196/151‑1 (MQ=255)

                                                                                                                                aaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTg  <  1:315627/151‑1 (MQ=255)

ACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTG  >  NC_000913/2548208‑2548486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A