Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,931,810 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,931,8100AT99.6% 604.7 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (113/141);  minor base G (0/1);  total (113/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,931,8110CG98.8% 596.1 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  major base G (110/140);  minor base T (1/2);  total (113/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,931,8120TA99.6% 602.9 / NA 255intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (112/142);  minor base C (1/0);  total (113/142)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 255 total aligned reads displayed.

CACACTTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTACTACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGAGCTACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCACTAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCC  >  CP042583/1931537‑1932091
                                                                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                                                                       
cactgatttATGAATACGACCATTCACCGGAACCGGCTCATTGAGAAGAATCGGGTAACACTGCGTACGCATCGTTGAACAATCAGGGCTGGTCATCAGAAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACAGCATGGTAAGAGTAAACAGACGAACCGGGCGGCACCACCGCTTGCCCCCTTGATCTGCCGAATGCGGCAATTTCCACCAGCGTGTTTGAACCACC‑CATCACGCCAACATCCGCCTGCATAACTTCGATACCAGCTTGTGAAAGCCTACGaaaa                                                                                                                                                                                                                                                                            >  2:1871605/7‑289 (MQ=255)
  ctgatttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGa                                                                                                                                                                                                                                                                          <  2:283036/285‑1 (MQ=255)
  cggatttATGACTACGCACATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGa                                                                                                                                                                                                                                                                          <  2:102321/285‑1 (MQ=255)
    gatttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGAtt                                                                                                                                                                                                                                                                        >  1:746622/3‑289 (MQ=255)
     atttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGAtt                                                                                                                                                                                                                                                                        >  1:2083994/2‑288 (MQ=255)
             aTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTATGAAAGCGTACGAAACGATTGCAGtgtg                                                                                                                                                                                                                                                                >  2:847286/1‑288 (MQ=255)
               aCGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCg                                                                                                                                                                                                                                                             >  1:675315/1‑289 (MQ=255)
               aCGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCg                                                                                                                                                                                                                                                             <  2:510538/289‑1 (MQ=255)
               aCGAACATTCACCGGAACCGGAGCATTGGGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCg                                                                                                                                                                                                                                                             <  2:2239758/289‑1 (MQ=255)
                 gACCTTCCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACAGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCGAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCgtg                                                                                                                                                                                                                                                           <  2:162923/289‑1 (MQ=255)
                      ttCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCTACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAgt                                                                                                                                                                                                                                                                   <  2:44704/276‑1 (MQ=255)
                      ttCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTCAAAGCGTACGAAAAAATTGCCGTGTGCCgggggcc                                                                                                                                                                                                                                                       >  2:1004087/1‑285 (MQ=255)
                           cGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAACGATTGCAGTGTTCCGTGGTGCTCAcc                                                                                                                                                                                                                                                  >  2:1462929/1‑288 (MQ=255)
                            ggAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGc                                                                                                                                                                                                                                                <  1:1942777/289‑1 (MQ=255)
                                   gCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGa                                                                                                                                                                                                                                                                          >  1:1418957/1‑256 (MQ=255)
                                   gCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGa                                                                                                                                                                                                                                                                          <  2:1418957/256‑1 (MQ=255)
                                         tGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACcatcat                                                                                                                                                                                                                                   <  1:1603182/289‑1 (MQ=255)
                                                     ggTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGgcgc                                                                                                                                                                                                                       >  1:1351779/1‑289 (MQ=255)
                                                          aaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCgcgttgc                                                                                                                                                                                                                  >  1:2134657/1‑289 (MQ=255)
                                                                       tcgttgaacAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCCGTTcgcg                                                                                                                                                                                                     >  2:487621/8‑289 (MQ=255)
                                                                         gttgaacAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGAt                                                                                                                                                                                                   <  2:751106/284‑1 (MQ=255)
                                                                         gttgaacAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAAGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGAt                                                                                                                                                                                                   <  1:466300/284‑1 (MQ=255)
                                                                              acAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACtt                                                                                                                                                                                              <  1:487621/289‑1 (MQ=255)
                                                                                  tCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTGTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCAt                                                                                                                                                                                          >  1:218621/1‑289 (MQ=255)
                                                                                  tCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCAt                                                                                                                                                                                          >  1:802530/1‑289 (MQ=255)
                                                                                  tCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGAGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCAt                                                                                                                                                                                          >  1:1458162/1‑289 (MQ=255)
                                                                                  tCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGAGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGAGTTGCGTTTCAGTTCGCGTTAAATTTCAt                                                                                                                                                                                          >  1:1742379/1‑289 (MQ=255)
                                                                                   ccGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATa                                                                                                                                                                                         <  1:1713293/289‑1 (MQ=255)
                                                                                            tcatcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCt                                                                                                                                                                                                                                                      >  1:1231458/1‑219 (MQ=255)
                                                                                               tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCGCCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggcgg                                                                                                                                                                             <  1:1544105/289‑1 (MQ=255)
                                                                                               tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggcgg                                                                                                                                                                             <  2:1829677/289‑1 (MQ=255)
                                                                                                     aTTCGCTGAATGGCGTGTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCggcaggca                                                                                                                                                                       <  2:218621/289‑1 (MQ=255)
                                                                                                        cccTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCAc                                                                                                                                                                      >  1:585528/3‑287 (MQ=255)
                                                                                                           tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCtt                                                                                                                                                                  <  2:855877/288‑1 (MQ=255)
                                                                                                           tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCtt                                                                                                                                                                  <  2:1351779/288‑1 (MQ=255)
                                                                                                              aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGa                                                                                                                                                               <  1:1004087/288‑1 (MQ=255)
                                                                                                              aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGAt                                                                                                                                                              <  1:1895651/289‑1 (MQ=255)
                                                                                                              aTGGCGTATTGGGGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGAt                                                                                                                                                              <  2:98150/289‑1 (MQ=255)
                                                                                                               tGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATc                                                                                                                                                             <  2:713657/289‑1 (MQ=255)
                                                                                                                    tATTGGTGAAGGTGATAACCGTATGGTGAGAGTAAACAGAGGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCAtt                                                                                                                                                         <  2:1226034/288‑1 (MQ=255)
                                                                                                                    gaTTGGTGAGGGTGTGCACAGCATGGTGAGATTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCAtt                                                                                                                                                         <  2:1567734/287‑1 (MQ=255)
                                                                                                                     aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTc                                                                                                                                                       <  1:499659/289‑1 (MQ=255)
                                                                                                                     aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTc                                                                                                                                                       <  1:1078665/289‑1 (MQ=255)
                                                                                                                     aTTGGTGAAGGTAATCACCGCCTGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTGGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTc                                                                                                                                                       <  2:1923419/289‑1 (MQ=255)
                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTtat                                                                                                                                                >  1:1005547/1‑289 (MQ=255)
                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTtat                                                                                                                                                >  1:1770097/1‑289 (MQ=255)
                                                                                                                              ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGcgcg                                                                                                                                                                                                                      >  2:110164/1‑217 (MQ=255)
                                                                                                                              ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAAc                                                                                                                                                                                                >  2:414374/1‑239 (MQ=255)
                                                                                                                              ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATTCCTCTGTGGCTGTCGGCACTCTTTGTTCCCTTTCAGGTTATAg                                                                                                                                              >  2:1241402/1‑289 (MQ=255)
                                                                                                                                tGATCACCGCATGGTGAGAGTAAACCGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAgg                                                                                                                                             <  1:1514605/288‑1 (MQ=255)
                                                                                                                                  aTCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGgc                                                                                                                                           >  1:1847406/1‑288 (MQ=255)
                                                                                                                                   tCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGgcgc                                                                                                                                         >  1:1122374/1‑289 (MQ=255)
                                                                                                                                     aCCGCATGGGGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACCTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGgcgcgc                                                                                                                                       <  2:1278566/289‑1 (MQ=255)
                                                                                                                                         cATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGc                                                                                                                                   >  2:802098/1‑289 (MQ=255)
                                                                                                                                               acaactAAACAGACGAACCGTGCGGCACCACCAGTTGCACCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCGGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTggg                                                                                                                              <  2:2134657/283‑1 (MQ=255)
                                                                                                                                                    tAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTTGCGGCAGGCCCTCTTCGATCCATTTCAGGTTATAGGTCGCGCAAGCGTGGGCCAGtt                                                                                                                        >  1:1031247/1‑289 (MQ=255)
                                                                                                                                                      aacagacgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGtt                                                                                                                        <  2:568683/278‑1 (MQ=255)
                                                                                                                                                       acagacgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTgg                                                                                                                     >  1:31202/9‑289 (MQ=255)
                                                                                                                                                           acgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTc                                                                                                                                                       <  2:1159843/247‑1 (MQ=255)
                                                                                                                                                           acgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTc                                                                                                                                                       >  1:1159843/5‑251 (MQ=255)
                                                                                                                                                            cgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCtgtg                                                                                                                                                                                 <  2:638434/221‑1 (MQ=255)
                                                                                                                                                            cgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCAtt                                                                                                                                                         <  1:380485/245‑1 (MQ=255)
                                                                                                                                                            cgaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCAtt                                                                                                                                                         >  2:380485/4‑248 (MQ=255)
                                                                                                                                                             gaaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCAt                                                                                                               <  1:915993/287‑1 (MQ=255)
                                                                                                                                                             aacacGTGCGGCACGACCAGTTGACCCCTGGATGTGTCGCTGGCGGCAATTTCCACCATCGTGGTTAAAACACCGCAACA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTGCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACGGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCAt                                                                                                               <  2:1228649/285‑1 (MQ=255)
                                                                                                                                                              aaCCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggcggc                                                                                                                                                                            >  1:869259/2‑227 (MQ=255)
                                                                                                                                                                 cGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGtt                                                                                                           <  1:553950/289‑1 (MQ=255)
                                                                                                                                                                 cGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGtt                                                                                                           <  1:104804/289‑1 (MQ=255)
                                                                                                                                                                 cGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGtt                                                                                                           <  2:675315/289‑1 (MQ=255)
                                                                                                                                                                 cGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGtt                                                                                                           <  1:1859687/289‑1 (MQ=255)
                                                                                                                                                                         caccccaGTTGCCCCCTGGATTTGGCGATTGCGGAAATTTCCCCCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCt                                                                                                   <  2:363962/285‑1 (MQ=255)
                                                                                                                                                                         accaccAGTTGCCCCCTGGATTTGGCGATGTCAGCAATTTCCACCAGGGTGGTTAAACAACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCt                                                                                                   <  2:1967806/289‑1 (MQ=255)
                                                                                                                                                                             ccagggtcccccTGGATTTGGCGATTGCGGCAATTTCCTCCAGCGGGGTTAAACCACCGCACCA‑GCCCAGATCTGGCTGCATAGTGTCGATACCGGTTTCTTAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCCGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACt                                                                                               <  2:1713396/282‑1 (MQ=255)
                                                                                                                                                                             ccaGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATCACTTTCATACTGCTGTGGCGGCAGGCACCTCTCCATCCCTTTCAGGTTATAGGGCGCGCCAGCGTGGGCCCTTTTGGTCGAATAGTTCACGTCCTgcc                                                                                                >  2:1917271/1‑286 (MQ=255)
                                                                                                                                                                                     ccccTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAACGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGCTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCCGTGGCGGCAGGCACTCTTCGCTCCATTGCAGGTTATAGGGCGCGCAAGCGTGGGCCAGGTTGGTCGCATAGTCCCCGGCCTGatccacccaac                                                                                       >  2:2031785/1‑279 (MQ=255)
                                                                                                                                                                                                     ttGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCtgtg                                                                                                                                                                                 >  1:249499/1‑183 (MQ=255)
                                                                                                                                                                                                      tGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGAt                                                                                                                                                                                                   <  1:1072494/164‑1 (MQ=255)
                                                                                                                                                                                                      tGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGAt                                                                                                                                                                                                   >  2:1072494/1‑164 (MQ=255)
                                                                                                                                                                                                                        cGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCAtttt                                                    >  1:1292735/1‑289 (MQ=255)
                                                                                                                                                                                                                         gTGGGTAAACCAACGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGTCTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCAttttt                                                   <  2:1849523/289‑1 (MQ=255)
                                                                                                                                                                                                                          tGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAATGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCAttttt                                                   >  2:2099043/1‑288 (MQ=255)
                                                                                                                                                                                                                            atgcaaCCACCGGACCA‑CCCAACATCCGGCTGCTGAATGTCTCTACCGGTGTATGAAAGAGTACGAAAAATGTGCAGTGGCCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACAGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCa                                                 <  2:237404/284‑1 (MQ=255)
                                                                                                                                                                                                                              tAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCTGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGc                                              <  1:787026/289‑1 (MQ=255)
                                                                                                                                                                                                                              tAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGGGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGc                                              <  1:1998614/289‑1 (MQ=255)
                                                                                                                                                                                                                               aaaCCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCa                                             <  1:1201742/289‑1 (MQ=255)
                                                                                                                                                                                                                               aaaCCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCa                                             <  2:805598/289‑1 (MQ=255)
                                                                                                                                                                                                                                  ccaccGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCAt                                                                                                               <  1:742925/220‑1 (MQ=255)
                                                                                                                                                                                                                                  ccaccGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCAt                                                                                                               >  2:742925/1‑220 (MQ=255)
                                                                                                                                                                                                                                      cGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCgt                                                                                                                                                                                                                                                            <  2:2086515/75‑1 (MQ=255)
                                                                                                                                                                                                                                      cGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGAt                                                                                                                                                                                                   <  1:643887/132‑1 (MQ=255)
                                                                                                                                                                                                                                         aCCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGAc                                   <  1:847286/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   cATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGc                          >  1:415449/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    aTCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTTGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCCTTTTTCACGCATATCCGCGACCATACCGTCa                         >  1:1646742/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           tGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTg                   <  1:820983/288‑1 (MQ=255)
                                                                                                                                                                                                                                                           tGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTg                   <  1:2246936/288‑1 (MQ=255)
                                                                                                                                                                                                                                                             cATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCCCACCGCATTTTTCACGCATATCCGCCACCATAGCGGCATCTTTTCgg                >  2:1133869/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  tGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGAt              <  2:774229/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGAt              >  1:774229/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                      gATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCa       <  1:1133869/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aTACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCg                                                                                                                  >  2:1542327/1‑181 (MQ=255)
                                                                                                                                                                                                                                                                                  |||                                                                                                                                                                                                                                                                                       
CACACTTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTACTACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGAGCTACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCA‑GCCAACATCCGGCTGCATAATGTCGATACCGGTTTCACTAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTCACCGCATTTTTCACGCATATCCGCGACCATAGCGGCATCTTTGCGGATCCCCGCATCGCC  >  CP042583/1931537‑1932091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A