Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,784,017 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,784,0170AT100.0% 862.7 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (169/188);  total (169/188)
*CP0425831,784,0180GC99.4% 852.1 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (169/186);  minor base A (0/2);  total (169/188)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,784,0190TA99.7% 853.7 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (168/188);  minor base C (1/0);  total (169/188)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 357 total aligned reads displayed.

TATCCTCGTGAGCGATTTTTACGAAGGGGGTAGTAGTAGTTTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAAGTTGGCTTGCGGAGAATCTTCAGAGTTGACATAACTTGCGGAGAATCTTCAGTCATGAATAGTCTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAG  >  CP042583/1783754‑1784298
                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
cttattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACt                                                                                                                                                                                                                                                                  <  1:2372909‑M2/53‑1 (MQ=255)
  tattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGa                                                                                                                                                                                                                                                                <  2:1830720‑M2/55‑1 (MQ=255)
   attgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGac                                                                                                                                                                                                                                                               >  1:2388899‑M2/234‑289 (MQ=255)
         caaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCa                                                                                                                                                                                                                                                         <  1:2033793‑M2/62‑1 (MQ=255)
                 gaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  1:2092534‑M2/70‑1 (MQ=255)
                   aaagcgtcagtaccccagtgagcgattttacctaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtttccagggcggcatcaaagtgctgtgactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACg                                                                                                                                                                                                                                               <  2:791672‑M2/72‑1 (MQ=255)
                                                                 atcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:236476‑M2/172‑272 (MQ=255)
                                                                 atcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTgg                                                                                                                                                                                                 >  1:910816‑M2/172‑289 (MQ=255)
                                                                   cattactgacgcatcaggtgaaaaagtttgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTCCAGTCATGAATTCCCTACGTCCCAAATTATTAGAACTGACAACGCAGGCCCTGACGGCGCTAAGCAACGCCGGTTTTGTTAAGCGAACTCTGAaggaaccgcaa                                                                                                                                                                                               >  2:1134455‑M2/170‑284 (MQ=255)
                                                                   cattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAg                                                                                                                                                                                                                                       >  2:1632456‑M2/170‑249 (MQ=255)
                                                                        ctgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATgg                                                                                                                                                                                          <  1:2182659‑M2/125‑1 (MQ=255)
                                                                        ctgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCCCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGGTAagccccggcttaaggccgtcctttatac                                                                                                                                                                                          >  2:1477483‑M2/165‑276 (MQ=255)
                                                                                tcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGCATTATTAGAACTGACACCGCAGGCCCCGACGGCGTTAAGCAATGCCGGTTTTGTTAATCGCAGGCTTAAGGAACTGGAAACTGGAAACGTc                                                                                                                                                                                   >  2:2486945‑M2/157‑288 (MQ=255)
                                                                                tcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGaaaa                                                                                                                                                                                             <  1:645705‑M2/122‑1 (MQ=255)
                                                                                tcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGaaaa                                                                                                                                                                                             >  2:645705‑M2/157‑278 (MQ=255)
                                                                                  aggtgaaaaagtgtgtccggagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTcc                                                                                                                                                                                  <  1:1562970‑M2/133‑1 (MQ=255)
                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCAt                                                                                                                                                                    >  2:2447878‑M2/143‑289 (MQ=255)
                                                                                               tgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGc                                                                                                                                                                                                                                                          >  2:1221652‑M2/142‑202 (MQ=255)
                                                                                               tgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGAt                                                                                                                                                                           >  2:3203750‑M2/142‑281 (MQ=255)
                                                                                               tgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGAt                                                                                                                                                                           <  1:3203750‑M2/140‑1 (MQ=255)
                                                                                                 tccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  1:3136218‑M2/70‑1 (MQ=255)
                                                                                                 tccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATgag                                                                                                                                                                 >  1:1604286‑M2/140‑289 (MQ=255)
                                                                                                 tccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATgag                                                                                                                                                                 >  2:1377358‑M2/140‑289 (MQ=255)
                                                                                                       gcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTa                                                                                                                                                                                                          >  2:1855724‑M2/134‑242 (MQ=255)
                                                                                                       gcggcatcaaagtgctgggactggcagcgctcgacagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGc                                                                                                                                                                                         >  1:2958507‑M2/134‑259 (MQ=255)
                                                                                                       gcggcatcaaagtgctgggactggcagcgctcgacagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGc                                                                                                                                                                                         <  2:2958507‑M2/126‑1 (MQ=255)
                                                                                                          gcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  1:1223615‑M2/131‑231 (MQ=255)
                                                                                                           catcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:744175‑M2/130‑230 (MQ=255)
                                                                                                           catcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCtt                                                                                                                                                       >  1:372383‑M2/130‑289 (MQ=255)
                                                                                                                       gggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCAcc                                                                                                                                            <  2:2589230‑M2/171‑1 (MQ=255)
                                                                                                                                 gcgcccgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggggcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACgg                                                                                                                                 <  2:2083764‑M2/182‑1 (MQ=255)
                                                                                                                                  cgctcgttaacaacgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgccccaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACgg                                                                                                                                 <  2:2034013‑M2/182‑1 (MQ=255)
                                                                                                                                   gctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACg                                                                                                                                  <  1:2199759‑M2/181‑1 (MQ=255)
                                                                                                                                   gctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACg                                                                                                                                  <  1:2310816‑M2/181‑1 (MQ=255)
                                                                                                                                    ctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:3036644‑M2/105‑205 (MQ=255)
                                                                                                                                    ctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  1:704272‑M2/105‑205 (MQ=255)
                                                                                                                                    ctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGgtg                                                                                                                               >  1:584264‑M2/105‑288 (MQ=255)
                                                                                                                                    ctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGgtg                                                                                                                               >  1:1813944‑M2/105‑288 (MQ=255)
                                                                                                                                               cgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTcc                                                                                                                                                                                  >  1:1111526‑M2/94‑226 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:200360‑M2/92‑192 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  1:200360‑M2/101‑1 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAacg                                                                                                                                                                                                                  >  2:1604507‑M2/92‑192 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGGTTTAATCGCCACCTTCAGTGACGGGGTGCGTacccatacg                                                                                                                 >  2:1346281‑M2/92‑285 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCTCCACCTTCAGTGACGGTGTCCGTACCCAGCTg                                                                                                                 >  1:1948747‑M2/92‑289 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGAc                                                                                                                                   <  1:1891348‑M2/180‑1 (MQ=255)
                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTg                                                                                                                 >  2:47874‑M2/92‑289 (MQ=255)
                                                                                                                                                         tgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGaa                                                                                                                                                                                                     >  2:1794472‑M2/84‑197 (MQ=255)
                                                                                                                                                          gctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              <  1:256009‑M2/41‑1 (MQ=255)
                                                                                                                                                          gctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              >  2:256009‑M2/83‑123 (MQ=255)
                                                                                                                                                              tgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACg                                                                                                                                                                                                                                               >  1:97014‑M2/79‑150 (MQ=255)
                                                                                                                                                              tgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAgg                                                                                                    <  1:48589‑M2/211‑1 (MQ=255)
                                                                                                                                                                accgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCg                                                                                                                                                                                                                               <  2:1673434‑M2/88‑1 (MQ=255)
                                                                                                                                                                 ccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAg                                                                                                                                       <  1:1050659‑M2/176‑1 (MQ=255)
                                                                                                                                                                 ccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCGTTAATCGCCACCTTCATTGACGGTGTCCGTACCCAGCTGCGTAACTGCCAGGCa                                                                                                  >  2:1185525‑M2/76‑288 (MQ=255)
                                                                                                                                                                    cgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCa                                                                                                                                                                                        >  2:2851319‑M2/73‑199 (MQ=255)
                                                                                                                                                                    cgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCa                                                                                                                                                                                        <  1:2851319‑M2/127‑1 (MQ=255)
                                                                                                                                                                    cgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCa                                                                                                  >  2:3094446‑M2/73‑285 (MQ=255)
                                                                                                                                                                       tacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAag                                                                                           <  1:1346281‑M2/220‑1 (MQ=255)
                                                                                                                                                                          ggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTa                                                                                                                                                                                                          <  1:3089094‑M2/109‑1 (MQ=255)
                                                                                                                                                                            cccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAgg                                                                                        <  1:2989375‑M2/223‑1 (MQ=255)
                                                                                                                                                                               aggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              >  2:486205‑M2/62‑102 (MQ=255)
                                                                                                                                                                               aggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCa                                                                                                                                                                                                                                                         <  2:679014‑M2/62‑1 (MQ=255)
                                                                                                                                                                                 gcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGga                                                                                                                                                                              >  1:1462078‑M2/60‑196 (MQ=255)
                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  1:2878674‑M2/55‑155 (MQ=255)
                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  2:2878674‑M2/101‑1 (MQ=255)
                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAgg                                                                                                                                                                                                       >  1:1630887‑M2/55‑166 (MQ=255)
                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTTTCCGTACCCAGCTGGCGAACGGCCCGGCACCGCACGAGGCCCAGTGCAGtt                                                                            >  2:1440940‑M2/55‑289 (MQ=255)
                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGtt                                                                            >  2:2529288‑M2/55‑289 (MQ=255)
                                                                                                                                                                                      ggttaaggtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACg                                                                                                                                  <  2:1942835‑M2/181‑1 (MQ=255)
                                                                                                                                                                                       gttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACGGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTCAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGaa                                                                                                            <  1:1699327‑M2/203‑1 (MQ=255)
                                                                                                                                                                                              tcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCgggg                                                                      <  1:1440940‑M2/241‑1 (MQ=255)
                                                                                                                                                                                               cggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCa                                                                   <  1:2477264‑M2/244‑1 (MQ=255)
                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCATTCCTGAATTCACTACGTCTGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCGGCCGAACGGCCAGGc                                                                                                   >  1:1210891‑M2/44‑255 (MQ=255)
                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGAc                                                                                                                                   <  2:2654697‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGAc                                                                                                                                   >  1:2654697‑M2/44‑223 (MQ=255)
                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAAc                                                                 >  1:176251‑M2/44‑289 (MQ=255)
                                                                                                                                                                                                  cgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAAc                                                                 <  1:770178‑M2/246‑1 (MQ=255)
                                                                                                                                                                                                            gccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGc                                                                                                                                                <  2:357332‑M2/167‑1 (MQ=255)
                                                                                                                                                                                                                 catgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTTTCCGTACCCATCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGGGCCGTTGCGGGGCCAACGGTATGTgacgtgctg                                                 >  2:1254815‑M2/28‑285 (MQ=255)
                                                                                                                                                                                                                     acgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATcgcg                                              <  2:176251‑M2/265‑1 (MQ=255)
                                                                                                                                                                                                                         cgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATg                                         <  1:2061283‑M2/270‑1 (MQ=255)
                                                                                                                                                                                                                          gggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGc                                        <  1:2515922‑M2/271‑1 (MQ=255)
                                                                                                                                                                                                                           ggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:3285668‑M2/18‑118 (MQ=255)
                                                                                                                                                                                                                           ggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  1:3285668‑M2/101‑1 (MQ=255)
                                                                                                                                                                                                                             cgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTg                                      <  2:2182021‑M2/273‑1 (MQ=255)
                                                                                                                                                                                                                                gctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGgtgt                                  >  1:387938‑M2/13‑289 (MQ=255)
                                                                                                                                                                                                                                  tggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGtt                                 <  2:1022270‑M2/278‑1 (MQ=255)
                                                                                                                                                                                                                                          tggcTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTAccc                                                                                                                      >  2:1087954/4‑195 (MQ=255)
                                                                                                                                                                                                                                           ggcTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCaa                       <  1:1282096/287‑1 (MQ=255)
                                                                                                                                                                                                                                           ggcTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCaa                       <  1:345141/287‑1 (MQ=255)
                                                                                                                                                                                                                                              ttGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGa                                           >  2:278414/1‑266 (MQ=255)
                                                                                                                                                                                                                                              ttGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGa                                           <  1:278414/266‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATcgcg                                              >  1:1833378/1‑262 (MQ=255)
                                                                                                                                                                                                                                                gCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACt                  >  1:1400549/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 cGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAAGTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGAGGCTTTAATCTCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACCGCCCGGCACTGACAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATAGCCAGTCATCGAAGGTCGCGGGTGTTAAGTTAGCAACGACtt                 >  2:943339/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        tCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGcca          <  1:2146620/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         cTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGcca          >  1:549300/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                cATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTACGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGCACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGGTATCAAAGACGTTGTGCCACCACCCAg  >  2:2150174/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                cATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACt     <  1:116888/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                cATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACt     <  1:1003692/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
TATCCTCGTGAGCGATTTTTACGAAGGGGGTAGTAGTAGTTTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAAGTTGGCTTGCGGAGAATCTTCAGAGTTGACATAACTTGCGGAGAATCTTCAGTCATGAATAGTCTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAG  >  CP042583/1783754‑1784298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A