Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,784,017 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,784,0170AT100.0% 730.3 / NA 304intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (145/159);  total (145/159)
*CP0425831,784,0180GC99.7% 723.4 / NA 304intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (145/158);  minor base A (0/1);  total (145/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,784,0190TA99.0% 725.8 / NA 304intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (143/158);  minor base C (2/1);  total (145/159)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

CAGCGAAAAGCGTCATTATCCTCGTGAGCGATTTTTACGAAGGGGGTAGTAGTAGTTTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAAGTTGGCTTGCGGAGAATCTTCAGAGTTGACATAACTTGCGGAGAATCTTCAGTCATGAATAGTCTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAG  >  CP042583/1783738‑1784298
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
gaagtatagtctgcaacttatttaacaaccagcgaaaagcgtcattatcctcgtgagcaatttttacgaaggtgtttcatcatcattactgacgcatcaggtgaaaaagtgtttccaaagctgcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgttactgcccaggcgctggttattgtcggcgcacaaatagccgccatgacgccgggcgatctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTc                                                                                                                                                                                                                                                                                  <  1:1456984‑M2/37‑1 (MQ=255)
    tgtggtcggaaacttattgaaaaaccagcgaaaagcgtcattattctcgtgagcgatttttacgaagggggttcatcatcattactgacgaatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagggctcgatagcaccgcaacaccttgcgatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              <  2:1937009‑M2/41‑1 (MQ=255)
     atggtcggcaacttattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              <  2:1250186‑M2/41‑1 (MQ=255)
      tggtcggcaacttattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAat                                                                                                                                                                                                                                                                            >  1:841228‑M2/247‑289 (MQ=255)
          cggcaacttattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagaaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAattatt                                                                                                                                                                                                                                                                        <  1:1908497‑M2/47‑1 (MQ=255)
                  tattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGa                                                                                                                                                                                                                                                                >  2:778220‑M2/235‑289 (MQ=255)
                  tattgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGa                                                                                                                                                                                                                                                                <  1:962118‑M2/55‑1 (MQ=255)
                     tgaacaaccagcgaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGaca                                                                                                                                                                                                                                                              >  1:2098740‑M2/232‑288 (MQ=255)
                           accagcgaaaagcgtcattatcctcgtgagcgagttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGa                                                                                                                                                                                                                                                                              <  1:1962529‑M2/41‑1 (MQ=255)
                                 gaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  1:1500676‑M2/70‑1 (MQ=255)
                                 gaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  1:914711‑M2/70‑1 (MQ=255)
                                 gaaaagcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  1:1753419‑M2/70‑1 (MQ=255)
                                   aatgcgacattatccgctgtagcgatttttacgaagggggttcgtcatcattacggacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgctagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 <  2:2203204‑M2/70‑1 (MQ=255)
                                   aaagcgtcattgtcagcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggctcaggcgctggttaatgtcggcgcacaaatagccgctatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACg                                                                                                                                                                                                                                               <  2:2104691‑M2/72‑1 (MQ=255)
                                     agcgtcattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGc                                                                                                                                                                                                                                             >  1:1477144‑M2/216‑289 (MQ=255)
                                          cattatcctcgtgagcgatttttacgaagggggttcatcatcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgacctggcagcatgGCTTGCGGAGAATCTTCAGTCATGACTTCACTACGCCCGGACTTATTAGAACGGACACCCCAGGCCCTGACGGCGTTaa                                                                                                                                                                                                                                        >  2:1543465‑M2/211‑289 (MQ=255)
                                                                                 atcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTTACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAATGAACTgg                                                                                                                                                                                                 >  2:1026636‑M2/172‑289 (MQ=255)
                                                                                 atcattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTgg                                                                                                                                                                                                 >  2:2254227‑M2/172‑289 (MQ=255)
                                                                                   cattactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTa                                                                                                                                                                                                          <  1:2207771‑M2/109‑1 (MQ=255)
                                                                                     ttactgacgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgccgggactggcagcgcacgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgccggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAATCGCAGTCTCTAGGAACTGGaaaa                                                                                                                                                                                             >  2:1592853‑M2/168‑289 (MQ=255)
                                                                                           gagcactaggtgaagaagggagtccagggcgggatcaaagtgctgggcctggcggagcacgatagcaccgcaacaagttgcgaggacggcgatacggccaaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcaagGCCTGCGGAGAATCTTCAGTCATGAATTCACTACGGCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCa                                                                                                                                                                                        <  2:1366051‑M2/127‑1 (MQ=255)
                                                                                           acgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:469306‑M2/162‑262 (MQ=255)
                                                                                           acgcatcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGGTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCaa                                                                                                                                                                                       >  1:1144796‑M2/162‑289 (MQ=255)
                                                                                              catcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACg                                                                                                                                                                                     >  1:2718817‑M2/159‑288 (MQ=255)
                                                                                                tcaggtgaaaaagtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTc                                                                                                                                                                                   >  1:74639‑M2/157‑288 (MQ=255)
                                                                                                 caggtgaaaaagtgtgtccagtgcggcatcaacgtgctgggactggcagcggtcgctagcaccgcaacaccttgctatgaccgcgatacggaccaggtgctggttaatgtcggcgcacaaaaagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTccc                                                                                                                                                                                 <  1:2600414‑M2/134‑1 (MQ=255)
                                                                                                            gtgtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  1:2240719‑M2/101‑1 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGtt                                                                                                                                                                                                                       >  1:632090‑M2/143‑238 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGtt                                                                                                                                                                                                                       <  2:632090‑M2/96‑1 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  2:1888957‑M2/101‑1 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTa                                                                                                                                                                                                          >  2:911136‑M2/143‑251 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGAt                                                                                                                                                                           >  1:1578543‑M2/143‑282 (MQ=255)
                                                                                                              gtgtccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgcccggcgagctggcatcgtgGCCTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTTGAACTGACACCGCAGGCCCTGCCCGAGTTAAGCAATGCCTGGTTCGTTAAGCGCATTTTGAAGGAACTGGAAAATGGCaccttcccggatgtccacca                                                                                                                                                                     >  2:2724182‑M2/143‑279 (MQ=255)
                                                                                                                 tccagagcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATgag                                                                                                                                                                 >  2:169447‑M2/140‑289 (MQ=255)
                                                                                                                      agcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAgg                                                                                                                                                                                                       <  1:233660‑M2/112‑1 (MQ=255)
                                                                                                                      agcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAgg                                                                                                                                                                                                       >  2:233660‑M2/135‑246 (MQ=255)
                                                                                                                      agcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAgg                                                                                                                                                                                                       >  2:1057306‑M2/135‑246 (MQ=255)
                                                                                                                       gcggcatcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAacga                                                                                                                                                            <  1:2441773‑M2/155‑1 (MQ=255)
                                                                                                                           catcaaagtgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:242039‑M2/130‑230 (MQ=255)
                                                                                                                                   tgctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGc                                                                                                                                                <  2:74639‑M2/167‑1 (MQ=255)
                                                                                                                                    gctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGcc                                                                                                                                               <  1:2153397‑M2/168‑1 (MQ=255)
                                                                                                                                     ctgggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCa                                                                                                                                              >  1:2361411‑M2/120‑288 (MQ=255)
                                                                                                                                        ggactggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCt                                                                                                                                           >  2:1747627‑M2/117‑288 (MQ=255)
                                                                                                                                             ggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTg                                                                                                                                     >  1:2350043‑M2/112‑289 (MQ=255)
                                                                                                                                             ggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTg                                                                                                                                     >  1:1668877‑M2/112‑289 (MQ=255)
                                                                                                                                             ggcagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTg                                                                                                                                     >  1:2403576‑M2/112‑289 (MQ=255)
                                                                                                                                               cagcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  2:2147190‑M2/101‑1 (MQ=255)
                                                                                                                                                 gcgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACgg                                                                                                                                 <  2:1668877‑M2/182‑1 (MQ=255)
                                                                                                                                                  cgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAattatt                                                                                                                                                                                                                                                                        <  2:421694‑M2/47‑1 (MQ=255)
                                                                                                                                                  cgctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAattatt                                                                                                                                                                                                                                                                        >  1:421694‑M2/107‑153 (MQ=255)
                                                                                                                                                   gctggatagcaccgcaacacatggtgctgactgcgttacggcccaggcgcgggttaatgacgacgcacacatagccgccatgacggcgggcgagctgggagtatgGGTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCAGGAATTATTAGAACTGACACCGCAGGCCGTGACGGCGTTAAGCAATGCCGGTTTTGTTGAGCACAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTCTAATCGCCACCTTCAGTGACg                                                                                                                                  <  2:2560614‑M2/181‑1 (MQ=255)
                                                                                                                                                   gctcgatagcaccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACg                                                                                                                                  <  2:2697129‑M2/181‑1 (MQ=255)
                                                                                                                                                         gacaaccgacacacctggcttagaccgcgttacgacccaggggctggtgaatgtgggcgtacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTAGTAGAACTGACACCGCAGGCCCTGATGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGt                                                                                                                          <  2:703990‑M2/189‑1 (MQ=255)
                                                                                                                                                            caccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  2:365610‑M2/97‑197 (MQ=255)
                                                                                                                                                            caccgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  <  1:365610‑M2/101‑1 (MQ=255)
                                                                                                                                                               cgcaacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACg                                                                                                                                  >  1:2273216‑M2/94‑274 (MQ=255)
                                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTg                                                                                                                 >  1:2636396‑M2/92‑289 (MQ=255)
                                                                                                                                                                 caacaccttgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGGCcgtacccctgt                                                                                                                  >  1:1742516‑M2/92‑284 (MQ=255)
                                                                                                                                                                         tgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  1:667137‑M2/84‑184 (MQ=255)
                                                                                                                                                                         tgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCa                                                                                                                                                                                        >  1:277309‑M2/84‑210 (MQ=255)
                                                                                                                                                                         tgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTaa                                                                                                                                                    >  1:467972‑M2/84‑246 (MQ=255)
                                                                                                                                                                         tgctatgaccgcgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTA‑TAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACgg                                                                                                         >  2:1396523‑M2/84‑288 (MQ=255)
                                                                                                                                                                                    cgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAacgacg                                                                                                                                                          >  1:870479‑M2/73‑229 (MQ=255)
                                                                                                                                                                                    cgatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGa                                                                                              >  2:1716788‑M2/73‑289 (MQ=255)
                                                                                                                                                                                     gatacggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCg                                                                                                                                                                                >  2:1874241‑M2/72‑206 (MQ=255)
                                                                                                                                                                                          ggcccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCa                                                                                                  <  1:1841028‑M2/213‑1 (MQ=255)
                                                                                                                                                                                            cccaggcgctggttaatgtcggcgctcaaagagccgccatgacgccgggcgatctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGgtgt                                                                                                                              <  1:1537949‑M2/185‑1 (MQ=255)
                                                                                                                                                                                            cccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGc                                                                                       >  1:1886919‑M2/65‑288 (MQ=255)
                                                                                                                                                                                            cccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGc                                                                                       >  1:761215‑M2/65‑288 (MQ=255)
                                                                                                                                                                                             ccaggcgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCt                                                                                      <  1:1028198‑M2/225‑1 (MQ=255)
                                                                                                                                                                                                  cgctggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAg                                                                                   <  1:1925017‑M2/228‑1 (MQ=255)
                                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGa                                                                                                                                                                                                                                                 >  2:311218‑M2/55‑124 (MQ=255)
                                                                                                                                                                                                      ggttaatgtcggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGtt                                                                                                                                                                                                                       <  2:1461626‑M2/96‑1 (MQ=255)
                                                                                                                                                                                                         taactgcgacgcacaaatagccgccatgaggccgggcgagctggcatcatgGCTTTCGGAGAATATTCAGTCATGAATTCACTACGACCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGc                                                                          <  2:1086367‑M2/237‑1 (MQ=255)
                                                                                                                                                                                                               cggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGcc                                                                    <  1:1427089‑M2/243‑1 (MQ=255)
                                                                                                                                                                                                               cggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCa                                                                   <  1:1008882‑M2/244‑1 (MQ=255)
                                                                                                                                                                                                               cggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCGTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCCCAGTGCAGTTGCGGGGCCa                                                                   >  1:1069893‑M2/46‑289 (MQ=255)
                                                                                                                                                                                                               cggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTA‑TAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCaa                                                                  <  1:1396523‑M2/244‑1 (MQ=255)
                                                                                                                                                                                                                ggcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCaa                                                                  <  1:2142715‑M2/245‑1 (MQ=255)
                                                                                                                                                                                                                 gcgcacacatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGCATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCaa                                                                  <  2:822874‑M2/245‑1 (MQ=255)
                                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCACTGCCGGTTTTGTTAAGCGCAGTCTTAAGG‑ACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCGTCAGTGACGGTGTCCGTAGCCAGCTGGCGAACGGCCAGGCACTGAAAAAGGCTCAGGGCAGTTGCGGGGCCAACg                                                                >  2:2034454‑M2/44‑289 (MQ=255)
                                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAgcg                                                                                                                                                                                                                  >  1:2036818‑M2/44‑144 (MQ=255)
                                                                                                                                                                                                                 gcgcacaaatagccgccatgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCAcc                                                                                                                                            <  2:2067528‑M2/171‑1 (MQ=255)
                                                                                                                                                                                                                                 catgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGcc                                                                                                       >  1:572501‑M2/28‑235 (MQ=255)
                                                                                                                                                                                                                                 catgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGcc                                                                                                       <  2:572501‑M2/208‑1 (MQ=255)
                                                                                                                                                                                                                                 catgacgccgggcgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCa                                                                                                  <  1:1368292‑M2/213‑1 (MQ=255)
                                                                                                                                                                                                                                             cgagctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGACCGTACCCAGCTGGCGAACCGCCAGGCACGTAAAGAGGCTCAGTGCCGTTGCGGGGCCAACGGGAGGtgacgtcctcgcggtattccgg                                     >  2:2354514‑M2/16‑280 (MQ=255)
                                                                                                                                                                                                                                               agctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGgtg                                   >  1:2627483‑M2/14‑289 (MQ=255)
                                                                                                                                                                                                                                                gctggcatcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGgtgt                                  >  1:607269‑M2/13‑289 (MQ=255)
                                                                                                                                                                                                                                                      atcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGtt                            <  1:169447‑M2/283‑1 (MQ=255)
                                                                                                                                                                                                                                                       tcatgGCTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACt                                                                                                >  1:511711‑M2/6‑220 (MQ=255)
                                                                                                                                                                                                                                                            gcTTGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCaa                       <  1:977428/287‑1 (MQ=255)
                                                                                                                                                                                                                                                              ttGCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCAcc                                                                                                                                            <  2:1628697/169‑1 (MQ=255)
                                                                                                                                                                                                                                                                gCGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTaa                               >  1:1600397/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                 cGGAGAATCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACtt                 >  1:787363/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       aTCTTCAGTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGcc           <  1:1201655/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aatCATGAATTACCTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACt     <  2:2627483/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gTCATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTc    >  1:1991781/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                cATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACt     <  1:2435618/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACt     <  2:1990432/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
CAGCGAAAAGCGTCATTATCCTCGTGAGCGATTTTTACGAAGGGGGTAGTAGTAGTTTACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAAGTTGGCTTGCGGAGAATCTTCAGAGTTGACATAACTTGCGGAGAATCTTCAGTCATGAATAGTCTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAG  >  CP042583/1783738‑1784298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A