Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 611,379 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583611,3790GC99.6% 594.6 / NA 254intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (112/140);  total (114/140)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*CP042583611,3800CG99.6% 595.0 / NA 254intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (113/139);  minor base T (0/1);  total (114/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583611,3810TA98.8% 591.0 / NA 254intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (112/138);  minor base G (1/2);  total (114/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 255 total aligned reads displayed.

AGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGACTAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGGCTCATATGAATATTAGCGGGAT‑CGCCGCTGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACC  >  CP042583/611101‑611660
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                        
aGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGAGAGCGAGAAAGGTACGCGACATATTAATATTAGCGGGAT‑CGCCCGAGCAAAttt                                                                                                                                                                                                                                                                                  >  1:696835/1‑289 (MQ=255)
       taaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CCCCAGACCAAATTTTGTTCt                                                                                                                                                                                                                                                                            >  2:1512473/1‑288 (MQ=255)
            ttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATTCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGccat                                                                                                                                                                                                                                                                       >  2:93178/1‑288 (MQ=255)
            ttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGccatc                                                                                                                                                                                                                                                                      <  2:1353099/289‑1 (MQ=255)
              aCCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGccatccc                                                                                                                                                                                                                                                                    >  2:361000/1‑289 (MQ=255)
              aCCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGccatccc                                                                                                                                                                                                                                                                    >  1:795710/1‑289 (MQ=255)
               ccAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGccatccca                                                                                                                                                                                                                                                                   <  1:902955/289‑1 (MQ=255)
                         tcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCgatgat                                                                                                                                                                                                                                                         <  1:1054135/289‑1 (MQ=255)
                         tcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCgatgat                                                                                                                                                                                                                                                         <  1:933098/289‑1 (MQ=255)
                              gttgtTTCCGGTTAAATGGAAAAATTGACGCTGGAATCCAGCACGCGAATGTTTTCCAGACCAGTAATGTGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCt                                                                                                                                                                                                                                                    <  1:1498531/287‑1 (MQ=255)
                               gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCgatgat                                                                                                                                                                                                                                                         >  1:1305755/1‑283 (MQ=255)
                               gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTc                                                                                                                                                                                                                                                   >  1:190810/1‑289 (MQ=255)
                                 gtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCa                                                                                                                                                                                                                                                  <  2:1851425/288‑1 (MQ=255)
                                  tttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAgg                                                                                                                                                                                                                                                >  2:1407166/1‑289 (MQ=255)
                                    tccggtgaaaTGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAgggg                                                                                                                                                                                                                                              >  1:1471961/9‑289 (MQ=255)
                                     ccggtgaaaTGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGt                                                                                                                                                                                                                                             <  2:68938/282‑1 (MQ=255)
                                     ccggtgaaaTGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGt                                                                                                                                                                                                                                             >  2:756039/8‑289 (MQ=255)
                                       ggtgaaaTGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTTGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCCCGATGGTCTGCCCAGGGGtat                                                                                                                                                                                                                                           >  2:212830/6‑289 (MQ=255)
                                       ggtgaaaTGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTg                                                                                                                                                                                                                                                                                >  1:712509/6‑252 (MQ=255)
                                                 aaaaaTTGACGCTGCGATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCaa                                                                                                                                                                                                                                  <  1:85518/288‑1 (MQ=255)
                                                          cGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTAc                                                                                                                                                                                                                        <  2:718289/288‑1 (MQ=255)
                                                          cGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTAc                                                                                                                                                                                                                        <  1:365395/288‑1 (MQ=255)
                                                          cGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGCCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTAc                                                                                                                                                                                                                        <  2:1448391/288‑1 (MQ=255)
                                                             tGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGt                                                                                                                                                                                                                     <  2:17808/288‑1 (MQ=255)
                                                              gCAACCCAGCCCGCGAATGTTTTCCAGAACAGTAATCTGATATTTCAGGTTGTGGGCAGTATGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCg                                                                                                                                                                                                                   <  2:1451243/289‑1 (MQ=255)
                                                                aaTCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGa                                                                                                                                                                                                                  <  1:1836437/288‑1 (MQ=255)
                                                                aaTCCAGAACGGGAATGTTTTCCAGACCAGTAATATGCTATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGAt                                                                                                                                                                                                                 <  2:1631312/289‑1 (MQ=255)
                                                                   ccAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATaca                                                                                                                                                                                                              <  1:638811/289‑1 (MQ=255)
                                                                   ccAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATaca                                                                                                                                                                                                              <  1:1428381/289‑1 (MQ=255)
                                                                       cACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGAGGAGGCATTTCTCGTATTTTGACGTATAACCGCATGCTAGCGAGAAAGGTACGCGACATATGAATATTAGACGGAA‑CGCCCGACCAAATTTTGTTCTGCCTTCACATCGCGATGATCAGATTAGGGGTATAACTATCAATGT‑TTTTAATGCCGATACCCttg                                                                                                                                                                                                          >  2:1908135/1‑287 (MQ=255)
                                                                         cgcgAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGCTGATCTGCCCAGGGGTAGAAATATCAATGT‑TCTTACTGTCGATACCCTGGtt                                                                                                                                                                                                        >  2:775621/1‑289 (MQ=255)
                                                                                             gTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAACTATCAATGT‑TCTTACTGTCGATACACTGGTTAGTTTCTATGCGAAttttt                                                                                                                                                                                     >  2:869143/1‑288 (MQ=255)
                                                                                                   tGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGa                                                                                                                                                                               >  2:844436/1‑288 (MQ=255)
                                                                                                     atatTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCCGACTg                                                                                                                                                                            >  1:1374339/1‑289 (MQ=255)
                                                                                                     atatTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTg                                                                                                                                                                            >  2:1173415/1‑289 (MQ=255)
                                                                                                        tttCAGGTTTTGGGCAGTATGGTCTTCGTTAACGCTACCGGCACCGTCACATTGAACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCTGAGGCATCTCGCGTATTTTGACGATTAACAGCTTTCCAGCCAGAAAAGTACGCGACATATGAATATGAGCGGGATGCACCG‑AGCACATGCTGTTATGCCATTCAATCGCGATGCTCTGCTCAGGGGTATAAAAATGAATGT‑TCGTACTTTCGATACCCCGGTTAGTGACGCGGCGAATTTTTCCTAATCTGGCa                                                                                                                                                                         >  2:24936/1‑289 (MQ=255)
                                                                                                        tttCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCa                                                                                                                                                                         >  2:1293595/1‑289 (MQ=255)
                                                                                                        tttCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCa                                                                                                                                                                         >  2:683683/1‑289 (MQ=255)
                                                                                                        tttCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCa                                                                                                                                                                         >  1:1443194/1‑289 (MQ=255)
                                                                                                        tttCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCCCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGGGTCGATGCGACTTTTTCCCAGACTgcct                                                                                                                                                                         >  2:1543499/1‑286 (MQ=255)
                                                                                                                tttGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATc                                                                                                                                                                 <  2:896233/289‑1 (MQ=255)
                                                                                                                     gCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCGGACTGGCATCGTAATCGaca                                                                                                                                                             <  1:869143/288‑1 (MQ=255)
                                                                                                                     gCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGaca                                                                                                                                                             <  1:171024/288‑1 (MQ=255)
                                                                                                                       atgcgggTGTTCGTTAACGCTCCCGGCACCGTCAAATTGCACGACGATATCGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTCCGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCg                                                                                                                                                          <  1:167440/285‑1 (MQ=255)
                                                                                                                              tCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTTTATg                                                                                                                                                   >  1:350855/1‑289 (MQ=255)
                                                                                                                              tCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATg                                                                                                                                                   >  2:382/1‑289 (MQ=255)
                                                                                                                                ttCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTc                                                                                                                                                 >  1:738403/1‑289 (MQ=255)
                                                                                                                                   gTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCg                                                                                                                                                                                                                   >  1:35949/1‑220 (MQ=255)
                                                                                                                                   gTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAAt                                                                                                                                                                  >  1:1176897/1‑269 (MQ=255)
                                                                                                                                   gTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACg                                                                                                                                              <  1:161568/289‑1 (MQ=255)
                                                                                                                                     tAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGcc                                                                                                                                            <  1:232607/289‑1 (MQ=255)
                                                                                                                                        cGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCgatgat                                                                                                                                                                                                                                                         <  2:316323/178‑1 (MQ=255)
                                                                                                                                        cGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCgatgat                                                                                                                                                                                                                                                         >  1:316323/1‑178 (MQ=255)
                                                                                                                                                     gTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAAt                                                                                                                                                                  <  2:1273668/251‑1 (MQ=255)
                                                                                                                                                          aTTGCACGACGATATAGTCGCTACGCGTACTTGGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTg                                                                                                                       <  2:1379981/289‑1 (MQ=255)
                                                                                                                                                               acgacgATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAccc                                                                                                                  <  1:1587456/289‑1 (MQ=255)
                                                                                                                                                               acgacgATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAccc                                                                                                                  <  2:795710/289‑1 (MQ=255)
                                                                                                                                                                cgacgaTATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAcccc                                                                                                                 <  1:339133/289‑1 (MQ=255)
                                                                                                                                                                      tataGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTACTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGAt                                                                                                            <  2:489664/288‑1 (MQ=255)
                                                                                                                                                                      tataGTAGCTAAGCGTACTTTGATAATCAGCCGGCGGCATTGCTCGTATGTTGACGTAGAACCGAGTGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATa                                                                                                           <  2:1669670/289‑1 (MQ=255)
                                                                                                                                                                         aGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAggg                                                                                                        >  2:972096/1‑289 (MQ=255)
                                                                                                                                                                         aGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAggg                                                                                                        >  1:939570/1‑289 (MQ=255)
                                                                                                                                                                             gCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGttt                                                                                                    >  2:351128/1‑289 (MQ=255)
                                                                                                                                                                             gCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGt                                                                                                      >  1:153871/1‑287 (MQ=255)
                                                                                                                                                                                aaGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACTCCGAGTTGATAATAACGATCCTGCACCCCCGGAAAGGGTTTTAc                                                                                                  >  2:1075731/1‑288 (MQ=255)
                                                                                                                                                                                aaGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                                                                 <  1:756039/289‑1 (MQ=255)
                                                                                                                                                                                aaGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAACCCCGGATAGGGTGTTAc                                                                                                  >  2:1208494/1‑288 (MQ=255)
                                                                                                                                                                                 aGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAccc                                                                                                <  2:515952/289‑1 (MQ=255)
                                                                                                                                                                                     tACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGc                                                                                            >  1:557417/1‑289 (MQ=255)
                                                                                                                                                                                                  aTGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                                                                 <  1:1659795/271‑1 (MQ=255)
                                                                                                                                                                                                    gcggcgGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCt                                                                             >  1:953336/1‑289 (MQ=255)
                                                                                                                                                                                                      ggcggcATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCGCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑GCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGCGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGCGATGCTCGCtt                                                                            >  1:58308/1‑288 (MQ=255)
                                                                                                                                                                                                          gcATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGGGTTGATAATAACGATCCTGCACCCCCGCATAGGGGTTTACCCAGGCTTAGACATGCTCGCTTTCaaa                                                                       >  1:1358166/1‑289 (MQ=255)
                                                                                                                                                                                                            aTTTCTCGTATTGTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGt                                                                     <  2:706958/289‑1 (MQ=255)
                                                                                                                                                                                                                  cGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTCGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGt                                                               <  2:1226780/289‑1 (MQ=255)
                                                                                                                                                                                                                      ttttGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGt                                                           <  1:1877861/289‑1 (MQ=255)
                                                                                                                                                                                                                       tttGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTa                                                          <  1:775621/289‑1 (MQ=255)
                                                                                                                                                                                                                       tttGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTa                                                          <  2:1887341/289‑1 (MQ=255)
                                                                                                                                                                                                                         tGACGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAtt                                                        <  1:1030918/289‑1 (MQ=255)
                                                                                                                                                                                                                           aCGTATAACCGCATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTAttgt                                                      >  2:338877/1‑289 (MQ=255)
                                                                                                                                                                                                                                      cATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                                                                 >  2:436074/1‑235 (MQ=255)
                                                                                                                                                                                                                                      cATGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTAcc                                                                                                 <  1:436074/235‑1 (MQ=255)
                                                                                                                                                                                                                                       aTGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAg                                           >  1:654748/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                         >  1:1099455/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tGCGAGCGAGAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGAc                                         >  1:1291924/1‑289 (MQ=255)
                                                                                                                                                                                                                                          cgagcgagAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCAccc                                                                                                                  <  1:69325/214‑1 (MQ=255)
                                                                                                                                                                                                                                              cgagAAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGa                                     >  2:1682542/1‑287 (MQ=255)
                                                                                                                                                                                                                                                agaAAGGTACGCGACATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAAttt                                 >  1:450029/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       tacgcgacATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCCAAGTTGCCGTTGGTATTGTTGTCCCAATAGAAGGGAATTTTAatat                           >  2:1650754/8‑288 (MQ=255)
                                                                                                                                                                                                                                                           cgacATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                                 <  2:540204/243‑1 (MQ=255)
                                                                                                                                                                                                                                                           cgacATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGcc                                                                 >  1:540204/4‑246 (MQ=255)
                                                                                                                                                                                                                                                            gacATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg                     <  1:382/287‑1 (MQ=255)
                                                                                                                                                                                                                                                            gacATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg                     <  1:1511676/287‑1 (MQ=255)
                                                                                                                                                                                                                                                             acATATGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAg                     <  2:390812/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                 atGAATATTAGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTc                 >  1:65102/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          aGCGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTa       >  1:1780309/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           gCGGGAT‑CGCCCGCGCCCATTTTGATATGCATTCCCATCGCGGTACTGATCTTAGCGGTATACATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTATTCCCAGTCTGGCAGCGTAATCGACACCGTTGTATGTCACGCTGATTTAATAATAACGATCCTGCACCCCCTCTTAGGGCGTTACCCCGGCTTCGCCATGCTCGCTTGCAACGTTGCCGTTGGTCTTGTTGTCACGCTAGACGGGAATTTTAATAACGTCAGAGTCGCCGATCTTa       >  2:644840/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                            cGGGAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCACAGATCTTAtt     >  1:1552422/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                               gAT‑CGCCCGAGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCAc   <  1:972096/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gttgacctgtGCAAAGTTGTTTATGCCTTCCCATCGCGATGTGCTGCTCAGGGGTATAAAGTTC‑ATGTGTCTTACTTTCGATACCCTTGGTAGTGTCGATGGGACTTTTTCGCAGACTGGCATCATAATCGACACAGTTGTATGTCCCGCCGGGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCAcc  <  2:604751/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                        
AGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGACTAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTATAACCGCATGCGAGCGAGAAAGGTACGGCTCATATGAATATTAGCGGGAT‑CGCCGCTGCAAATTTTGTTCTGCCATCCCATCGCGATGATCTGCTCAGGGGTATAAATATCAATGT‑TCTTACTGTCGATACACTGGTTAGTGTCGATGCGACTTTTTCCCAGACTGGCATCGTAATCGACACCGTTGTATGTCACGCCGAGTTGATAATAACGATCCTGCACCCCCGGATAGGGGTTTACCCAGGCGTAGACATGCTCGCTTTCAAAGTTGCCGTTGGTATTGTTGTCACAATAGACGGGAATTTTAATATCGTCAGATTCCCAGATCTTATCACC  >  CP042583/611101‑611660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A