Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,463,349 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,463,3490AT100.0% 503.2 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (113/100);  total (113/100)
*CP0425831,463,3500GC100.0% 502.9 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (113/100);  total (113/100)
*CP0425831,463,3510CG99.5% 502.9 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (113/99);  minor base T (0/1);  total (113/100)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 213 total aligned reads displayed.

GAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGAGCGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTAAGTGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGC  >  CP042583/1463069‑1463625
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                  
gAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGAc                                                                                                                                                                                                                                                                              >  1:1686137/1‑289 (MQ=255)
              tgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGTCGCTAATTCCCCTTTCAACCACATCTCCCGCTTTAAACGCATCGGGAAGTGAGCCTAACGCATGTCGGCTGACAAATTTGATGccct                                                                                                                                                                                                                                                                >  2:94810/1‑287 (MQ=255)
                tctcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGAGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGCTGAAAAATTTGATGCCCTtg                                                                                                                                                                                                                                                              >  2:582733/1‑289 (MQ=255)
                    cGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          <  1:1908496/289‑1 (MQ=255)
                    cGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          <  1:279208/289‑1 (MQ=255)
                    cGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          <  1:715340/289‑1 (MQ=255)
                    cGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          <  2:1686137/289‑1 (MQ=255)
                         tgtTGCTGAAATCCGACCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGt                                                                                                                                                                                                                                                      <  1:1663520/288‑1 (MQ=255)
                               tGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGc                                                                                                                                                                                                                                                <  2:564559/288‑1 (MQ=255)
                                 aaaTCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGtt                                                                                                                                                                                                                                             >  2:1032451/1‑289 (MQ=255)
                                        aTCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTa                                                                                                                                                                                                                                      >  2:1541797/1‑289 (MQ=255)
                                             tACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTTCGGGCGTTGCTGGTAGCGtt                                                                                                                                                                                                                                 >  2:1579939/1‑289 (MQ=255)
                                                    cGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGc                                                                                                                                                                                                                            >  1:525878/1‑287 (MQ=255)
                                                    cGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCa                                                                                                                                                                                                                           <  1:321853/288‑1 (MQ=255)
                                                    cGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCa                                                                                                                                                                                                                           <  1:206603/288‑1 (MQ=255)
                                                    cGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCa                                                                                                                                                                                                                           <  1:1705186/288‑1 (MQ=255)
                                                       tGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGCAGGGAGCATAACGCATGTCGGCTGCCAAAATTGATGCCATTGTGGTCGGTCCGGGCCTTGCTGGGTGCGGTTCCGcgcgtg                                                                                                                                                                                                                       >  2:1118571/1‑284 (MQ=255)
                                                       tGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCACGTCGGATTACAAATTTGATGCCATTGGGGGCGGAGCGGGCGTTGCGGGTCGCGTCGCCGCACTgg                                                                                                                                                                                                                       >  2:1193866/1‑289 (MQ=255)
                                                         gggATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTc                                                                                                                                                                                                                     >  1:1073398/1‑289 (MQ=255)
                                                          tgATCTCCGAGCAGATCGAGCATATGGTTGGCGGAAAAGGCGCAAGAGTGATTGTAGCCATAAATAAAGATAAAAACGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTc                                                                                                                                                                                                                     <  1:46639/287‑1 (MQ=255)
                                                               tcCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGCGAGCCTAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGCGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGc                                                                                                                                                                                                                >  2:1740114/1‑288 (MQ=255)
                                                                    gCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAg                                                                                                                                                                                                           <  1:327588/288‑1 (MQ=255)
                                                                    gCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAg                                                                                                                                                                                                           <  1:306502/288‑1 (MQ=255)
                                                                     cAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGc                                                                                                                                                                                                          <  1:1032451/288‑1 (MQ=255)
                                                                        aTCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGt                                                                                                                                                                                                                      >  1:150521/1‑273 (MQ=255)
                                                                         tCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCgg                                                                                                                                                                                                       >  1:1013066/1‑287 (MQ=255)
                                                                              cATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACTAGCCGGGCTGGAt                                                                                                                                                                                                >  1:989906/1‑289 (MQ=255)
                                                                               atatGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTgg                                                                                                                                                                                                  <  2:412632/286‑1 (MQ=255)
                                                                               atatGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTgg                                                                                                                                                                                                  <  1:1571698/286‑1 (MQ=255)
                                                                                  tGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATTTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCCCTGGTCCTGGCCAGAACCGGGCTGGAttg                                                                                                                                                                                              >  2:1951814/1‑285 (MQ=255)
                                                                                   ggTTGGCGGCAACGGCGCATAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGt                                                                                                                                                                                              >  1:91585/1‑286 (MQ=255)
                                                                                   ggTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCAGGGCAGGAGCCGGGCTGGATgg                                                                                                                                                                                              >  2:811283/1‑285 (MQ=255)
                                                                                   ggTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTACTGGCACGAGCCGGGCTGGATgc                                                                                                                                                                                              >  2:1917874/1‑285 (MQ=255)
                                                                                   ggTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTCCCGCACTGGTCATGGCCCGCGCCGGGCTGGATGTccg                                                                                                                                                                                           >  2:1767814/1‑288 (MQ=255)
                                                                                       ggcggcAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGc                                                                                                                                                                                                                >  2:1672920/1‑264 (MQ=255)
                                                                                        gcggcAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAg                                                                                                                                                                                                                                     >  2:1649750/1‑242 (MQ=255)
                                                                                              aCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAAc                                                                                                                                                                                >  2:75551/1‑289 (MQ=255)
                                                                                                     aaaaGTGATTCTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGa                                                                                                                                                                         <  1:1143927/289‑1 (MQ=255)
                                                                                                      aaaGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGAc                                                                                                                                                                        <  2:208830/289‑1 (MQ=255)
                                                                                                            aTTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCggg                                                                                                                                                                                                                                                 <  2:3257/210‑1 (MQ=255)
                                                                                                                              gATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGa                                                                                                                                                                         >  2:477624/1‑264 (MQ=255)
                                                                                                                              gATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGa                                                                                                                                                                         <  1:477624/264‑1 (MQ=255)
                                                                                                                               aTAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACcg                                                                                                                                               >  1:390069/1‑289 (MQ=255)
                                                                                                                                  aaaaTGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCg                                                                                                                                                                                                                                               <  2:1326741/190‑1 (MQ=255)
                                                                                                                                     aTGGGCCAATATGCAACTATGCCGACGACGTGCGGGTGGAC‑GATATCTACAAGGTCGTCCCTGCCCTGATTGGCCGGTGGGGCCGCTAATGCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGGGCAGAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCggcgggc                                                                                                                                         <  2:1150996/289‑1 (MQ=255)
                                                                                                                                     aTGCGCCGAGCTTAAACTATCCCGACTTGGGTCTGGTGGGC‑CATCTCTACACAGTCGTCCCTGCCCTGATTGGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCCTAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGAACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCggcgggc                                                                                                                                         <  2:1834285/289‑1 (MQ=255)
                                                                                                                                     aTGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCggcgggc                                                                                                                                         <  1:1882186/289‑1 (MQ=255)
                                                                                                                                        cgcCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACCAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGGGGGAAGGGAGCCTAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGTTTCGGGCGTTGATGGTAGCGTTGCCGCCCTGGTCATGGCACGAGCTGGGTCGGACTTACTGGTTGGAGGACGTCGCGACAGCGCCGGTTGATGACACATGACcggtcgtggtg                                                                                                                                      >  2:1900422/1‑281 (MQ=255)
                                                                                                                                        cgcCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCggcgggcg                                                                                                                                        >  1:138969/1‑287 (MQ=255)
                                                                                                                                         gcCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCt                                                                                                                                     >  1:1729842/1‑289 (MQ=255)
                                                                                                                                          ccAATCTTCAACTATGCCTGCTACGGTCTGGTGGGC‑GCTATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCtt                                                                                                                                    <  2:542169/289‑1 (MQ=255)
                                                                                                                                            aattctCAACTATGCCGAATACGGCCTGGTTGGC‑GATTTCGACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGGGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAACCGCGGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGGGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTa                                                                                                                                  <  2:1483196/284‑1 (MQ=255)
                                                                                                                                              tCTTCAACTATGCCGACTACGGACTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGCCTTTAt                                                                                                                                 >  1:1115646/1‑288 (MQ=255)
                                                                                                                                                ttCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGcc                                                                                                                              <  1:761015/289‑1 (MQ=255)
                                                                                                                                                  cAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCca                                                                                                                            <  1:811283/289‑1 (MQ=255)
                                                                                                                                                  cAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCca                                                                                                                            <  1:699219/289‑1 (MQ=255)
                                                                                                                                                    aCTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCcaca                                                                                                                          <  1:580635/289‑1 (MQ=255)
                                                                                                                                                        tGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCca                                                                                                                            <  2:797025/283‑1 (MQ=255)
                                                                                                                                                        tGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACt                                                                                                                      >  1:35178/1‑289 (MQ=255)
                                                                                                                                                          ccGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTg                                                                                                                    >  1:376010/1‑289 (MQ=255)
                                                                                                                                                           cGACTACGTGTTGGTGGGCGGATGT‑TCCAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGa                                                                                                                   <  2:1060718/289‑1 (MQ=255)
                                                                                                                                                             aCTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGaa                                                                                                                  >  1:1150293/1‑288 (MQ=255)
                                                                                                                                                               tACGGTCTGGTGGGC‑GATATCTACAAAGTCGGCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCTCATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCa                                                                                                               <  1:1649528/289‑1 (MQ=255)
                                                                                                                                                                aCGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGGCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCaa                                                                                                              <  2:1531766/289‑1 (MQ=255)
                                                                                                                                                                        gtggGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCa                                                                                                      >  1:1820610/1‑289 (MQ=255)
                                                                                                                                                                          gggC‑GATATCTACGAAGTCGGCCCGGCCCTGCTTAGGCAGTTGGGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAgg                                                                                                    <  2:1881311/289‑1 (MQ=255)
                                                                                                                                                                            ggcgtattctcCAAAGTCTGCCCTGCCCTGAGTCGCCAGTTGAGCCGCTAATTCCCCTTTCAACCCCATCCCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAgg                                                                                                    <  2:684240/281‑1 (MQ=255)
                                                                                                                                                                            gC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCt                                                                                                  >  1:1313116/1‑289 (MQ=255)
                                                                                                                                                                             c‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCtt                                                                                                 >  1:1403954/1‑289 (MQ=255)
                                                                                                                                                                                  tgcTACAAGGTCGTCCCGGCCCTGCTTAGCCAGTTCAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCa                                                                                             <  2:917556/287‑1 (MQ=255)
                                                                                                                                                                                   tCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAg                                                                                            >  1:253553/1‑289 (MQ=255)
                                                                                                                                                                                       cAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTatc                                                                                        <  1:1541797/289‑3 (MQ=255)
                                                                                                                                                                                        aaaGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACcgg                                                                                                                                              >  1:224860/1‑234 (MQ=255)
                                                                                                                                                                                        aaaGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCACTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACcgg                                                                                                                                              <  2:224860/234‑1 (MQ=255)
                                                                                                                                                                                         aaGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTatcag                                                                                      <  1:1865857/289‑5 (MQ=255)
                                                                                                                                                                                            tcgtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGt                                                                                                                                                                                                                                              >  1:676521/1‑134 (MQ=255)
                                                                                                                                                                                             cgtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          <  2:135241/121‑1 (MQ=255)
                                                                                                                                                                                             cgtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGt                                                                                                                                                                                                                                                          >  1:135241/1‑121 (MQ=255)
                                                                                                                                                                                             cgtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGCGAGAACGCGGCGACATTGCCGGAGGTATAAACATTACCGGCGGGCGTCTTTTTTCACACGCCAGTTAACGAATTATTCCACGTCTTGCAGTaatcggccc                                                                                  >  2:48708/1‑282 (MQ=255)
                                                                                                                                                                                               tcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTcc                                                                                                                                                                                            >  2:1093329/1‑181 (MQ=255)
                                                                                                                                                                                               tcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGAACGGCGGGCGTCTTTATGCCCCCACACTTGAAGCAATCATTCCAGGCTTTGCAGTCACAGCGCCgg                                                                                >  2:465966/1‑289 (MQ=255)
                                                                                                                                                                                                    gccctgATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGaa                                                                           >  1:1939134/1‑289 (MQ=255)
                                                                                                                                                                                                      cctgATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAAc                                                                          >  1:832784/1‑288 (MQ=255)
                                                                                                                                                                                                               ccAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATTTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACCCTGGCAGCAAACATTCCCGGCTTTGCATTCTCCGCGCCGGTAGAACGAAAGGTcac                                                                >  2:1462616/1‑289 (MQ=255)
                                                                                                                                                                                                               ccAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTcac                                                                >  1:1584669/1‑289 (MQ=255)
                                                                                                                                                                                                                    tGAGCCGCTAAGTCCCCTTTAAACCACAGCTCCCGCTGTAAACGCAGCGGGAAGGGGGCATAACGCATGTCGGATGACAAATTTGCTGCCATTGTGGTCGGTGCGGGCGTTGCTAGTGGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCacac                                                              <  2:253553/286‑1 (MQ=255)
                                                                                                                                                                                                                            tAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAAtc                                                   <  1:1193866/289‑1 (MQ=255)
                                                                                                                                                                                                                                   cccttCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCTGATGACAAATTTGATGCCATTGTGGTCGTTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCAAGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCcttct                                            <  1:1171787/286‑1 (MQ=255)
                                                                                                                                                                                                                                      ttCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTaa                                         <  1:1568132/289‑1 (MQ=255)
                                                                                                                                                                                                                                      ttCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGACCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTa                                          >  1:796885/1‑288 (MQ=255)
                                                                                                                                                                                                                                              caTCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCgaagaa                                 >  1:434026/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 ctcCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAgc                              >  1:517663/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    ccGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCCATCATTCCAGGCTTTGCAGTATCAGCGCCGGGAGAACGCAAGGCCCACCGCCAGGAAATCCCCTTCTTAACCGAAGaaaaccc                            >  2:1418239/1‑284 (MQ=255)
                                                                                                                                                                                                                                                        tGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCACCGCCGGTAGAACGCAAGGTCACACGCGCGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGtt                        >  1:1778273/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                 aGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCgg                                                                                                                                                                <  1:876750/143‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCgg                                                                                                                                                                >  2:876750/1‑143 (MQ=255)
                                                                                                                                                                                                                                                                  gCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCCTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAttt             >  1:1809206/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    gggAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCctt                                              >  1:777259/1‑254 (MQ=255)
                                                                                                                                                                                                                                                                           gatctgaaCGCATGTCGGATGACCAATTTGAGGCATTGGTGGGCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGAGAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACcgcg     <  2:765955/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGTCCACACACCTGAAGCAATCATTCCAGGCTTTGCAGTAACAGCGCCGGTAAAACGCAAGGTCACACGCGCGAAAAGCTCCTTCTTAAACGAAGAAAGCGCCGTTACCCTCGAATTTTACCTCTagc  >  2:1803849/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                  
GAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGC‑GATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGAGCGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTAAGTGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGC  >  CP042583/1463069‑1463625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A