Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,924,695 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,924,6950AT99.0% 472.9 / NA 203intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (95/106);  minor base C (2/0);  total (97/106)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,924,6960GC99.5% 467.4 / NA 203intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (96/105);  total (97/106)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,924,6970CG98.5% 463.4 / NA 203intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base G (94/106);  total (97/106)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGAAGCCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTAGCCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGA  >  CP042583/1924417‑1924974
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                     
     gAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTacca                                                                                                                                                                                                                                                                          <  1:899695/289‑1 (MQ=255)
     gAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTacc                                                                                                                                                                                                                                                                           >  2:975594/1‑288 (MQ=255)
      agtgaaCGGGGCTGATTCGTGAAGGGGCGACGGGGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCCGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGAACTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTac                                                                                                                                                                                                                                                                            <  2:240901/281‑1 (MQ=255)
           aCGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTacc                                                                                                                                                                                                                                                                           >  2:829529/1‑282 (MQ=255)
           aCGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTacc                                                                                                                                                                                                                                                                           <  1:829529/282‑1 (MQ=255)
           aCGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCtt                                                                                                                                                                                                                                                                    >  2:1926180/1‑289 (MQ=255)
           aCGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATC‑GGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCttt                                                                                                                                                                                                                                                                   <  1:35451/289‑1 (MQ=255)
               ggCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTAc                                                                                                                                                                                                                                                                 <  2:251030/288‑1 (MQ=255)
               ggCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTAc                                                                                                                                                                                                                                                                 <  1:271192/288‑1 (MQ=255)
               ggCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGACGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTAc                                                                                                                                                                                                                                                                 <  1:1283393/288‑1 (MQ=255)
                 cTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTTATCATGGATCACCGCATTAACCAGCATGTGATCAACCGTTGCCGTAAACCTTCCCACGCCGATATTC‑CGTGCACTGGCGTTACCTTTCCGCTGATGGGTACCACCCCtttcactt                                                                                                                                                                                                                                                              >  2:892103/1‑284 (MQ=255)
                  tGATTCGTGAGGTCTCGAAGGTGTGCGGTGTTCGTGTCCGTAACCATCTCACCGGCGAAACTCAGGCACTGCATGCACCTGTAGTGGTTAATGCCGCTGGGATCTGGGGGCAACACTTTGCCGAATATGCCGGTCTGCGCATTCGCATGTGCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTa                                                                                                                                                                                                                                                              <  2:91356/288‑1 (MQ=255)
                  tGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTa                                                                                                                                                                                                                                                              <  1:102497/288‑1 (MQ=255)
                      tCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGAt                                                                                                                                                                                                                                                         <  1:134164/289‑1 (MQ=255)
                         tGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGAACACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATGTTCTGGTGC‑CTGGCCATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTAc                                                                                                                                                                                                                                                      >  2:972596/1‑289 (MQ=255)
                           aaGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGGGATCAACCGCTGCCGTAAACCTTCCGACGCCGATAGTCTGGTGC‑CTGGCGATACCCTTTGCCTGGTTGGGACCACCTCTTGACGTATTGATTaac                                                                                                                                                                                                                                                     >  2:431434/1‑286 (MQ=255)
                            aGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAAc                                                                                                                                                                                                                                                   <  1:1601574/289‑1 (MQ=255)
                                gcgACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACgaga                                                                                                                                                                                                                                               <  1:321227/289‑1 (MQ=255)
                                     ggTGTGCGGTGTTGGGGTACGTAACCATCTCACCGCCGGAACTCATGCCCTACATGCAGCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACGTTGCCGAATGTGCCGATCTGCGCATTCGCATGTTCTCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTATACGTATTGATTACAACGAGATTGa                                                                                                                                                                                                                                           <  2:1177644/288‑1 (MQ=255)
                                         tgCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATCCCATTTCGCTGATTGGTACCACCCCTTTCCTTATTGTTTACCACGAAATTGACGaaa                                                                                                                                                                                                                                      >  2:1028150/1‑287 (MQ=255)
                                                ttggtgcACGCAACCGTCTCCCCGGCGAAACTCCAGCCCTCCGTGCTCCTGTCGTGGTTAATGCCGCTGGGGTCTGGGGGCAACACATTGCCGACTATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                                                                                               <  2:1584407/286‑1 (MQ=255)
                                                ttCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTac                                                                                                                                                                                                                                                                            <  1:903894/244‑1 (MQ=255)
                                                 tCGTGTACGTAACCATCTCACCGGCGGAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCTATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGAGCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGTCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAgct                                                                                                                                                                                                                              >  1:1177644/1‑287 (MQ=255)
                                                 tCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTac                                                                                                                                                                                                                                                                            <  1:675355/243‑1 (MQ=255)
                                                 tCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                                                                                               <  1:1904926/288‑1 (MQ=255)
                                                 tCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                                                                                               <  1:422623/288‑1 (MQ=255)
                                                 tCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTg                                                                                                                                                                                                                              >  2:563306/1‑289 (MQ=255)
                                                   gtgtACGTAACCATCCCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTaccacc                                                                                                                                                                                                                                                                        >  2:725598/1‑245 (MQ=255)
                                                        cGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCag                                                                                                                                                                                                                       >  1:1496888/1‑289 (MQ=255)
                                                           aaacATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaa                                                                                                                                                                                                                     <  2:1627911/285‑1 (MQ=255)
                                                           aaCCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaa                                                                                                                                                                                                                     <  2:701741/288‑1 (MQ=255)
                                                           aaCCATCTCACCGGCGAAACTCAGCCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaa                                                                                                                                                                                                                     <  1:1095561/288‑1 (MQ=255)
                                                            cccatgcgaCCGGCGAAACCCAGGCCCTGTAGGAACAGGTCGTGGGTAATTCCGCTGGGACCTGGGGGCAACACATTGCCGAATATGCCGTTCTTCCCATTCGCATGTTCCCGTCGAAAGGCTCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTAGCTGATTGGTACCACCTCTTTACGTATGGATTACAACGAGATTGACGATAATCGAGTGACGGCagaaga                                                                                                                                                                                                                   <  2:67970/281‑1 (MQ=255)
                                                            aCCATCTCACCGGCGAAACTCAGGACCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACACTGCCGAAAATGACGACCTGCGCCTTCGCATGTTCCCGGCGAAAGGCGCGCTGCCGCTCCATGATCACCGCAGTAACCAGCATGCGATCCACCGCTCCCGGAAACCTTCCGACGCCGATCTTCTGGTGT‑CTGGCGACACCATTTCCACTACTGTGACCTCCTCTTTCCGTATTTATTACAACGATCTTTACGATACTCGaattacctcaatagt                                                                                                                                                                                                                   >  2:1670731/1‑275 (MQ=255)
                                                               aTCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGt                                                                                                                                                                                                                <  1:1684544/289‑1 (MQ=255)
                                                               aTCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGt                                                                                                                                                                                                                <  1:22720/289‑1 (MQ=255)
                                                               aTCTCACCGGCGAAACTCAGGCCCTTCACGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGt                                                                                                                                                                                                                <  2:624594/289‑1 (MQ=255)
                                                                tctcACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGtt                                                                                                                                                                                                               >  1:706873/1‑289 (MQ=255)
                                                                 ctcACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAATCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGTTTCAAACGGGATTGACGATAATCCACGCACGCCAGAAGAGGttc                                                                                                                                                                                                              >  2:1807825/1‑288 (MQ=255)
                                                                      cGGGGAAACTCAGGACCTTCATGCACCTGTAGTGGTTAATGCCGCTGGGGTCTGGGGGAAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATAtt                                                                                                                                                                                                         <  2:375234/289‑1 (MQ=255)
                                                                      cGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATAtt                                                                                                                                                                                                         <  1:807964/289‑1 (MQ=255)
                                                                      cGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATAtt                                                                                                                                                                                                         >  1:1136782/1‑289 (MQ=255)
                                                                          gAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTctg                                                                                                                                                                                                      <  1:1928461/288‑1 (MQ=255)
                                                                             aCTCAGGCCCTTCATGCACCTGTCGTTGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGt                                                                                                                                                                                                                <  2:1428058/275‑1 (MQ=255)
                                                                                 aGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGa                                                                                                                                                                                              <  2:59455/289‑1 (MQ=255)
                                                                                         cATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTTC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGaaa                                                                                                                                                                                      >  2:1928197/1‑289 (MQ=255)
                                                                                                cTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAAAGGTTGATATTCTGCTGCGGTAAGGGGAAAAACTgg                                                                                                                                                                                >  2:1512995/1‑288 (MQ=255)
                                                                                                  gTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGcc                                                                                                                                                                              >  2:484613/1‑288 (MQ=255)
                                                                                                      tGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGt                                                                                                                                                                         >  2:1950553/1‑289 (MQ=255)
                                                                                                          tAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATg                                                                                                                                                                     >  1:1415349/1‑289 (MQ=255)
                                                                                                                 gCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATTGAGTGACGGCAGAAGGGGTTGATATTCTGCCGCGTGAAGGGGAAAAACTGGCCCCCGTGAGGGCGtaaa                                                                                                                                                              >  2:1529820/1‑289 (MQ=255)
                                                                                                                                cAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCgg                                                                                                                                                <  1:1012104/288‑1 (MQ=255)
                                                                                                                                cAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCgg                                                                                                                                                <  1:885281/288‑1 (MQ=255)
                                                                                                                                 aaCACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGCTCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGCGGTTGAGTTTCTGCTGCGTGAAGGGGAAAAACTGGCCCCAGGTATGGCGAAACCGCGCATTTTGCggg                                                                                                                                               >  2:1114469/1‑288 (MQ=255)
                                                                                                                                 aaCACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGc                                                                                                                                              <  1:566472/289‑1 (MQ=255)
                                                                                                                                      aTTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                                                                                               >  2:1711222/1‑203 (MQ=255)
                                                                                                                                        tGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGACAACGCGCATTTTGCGGGCCTATTcg                                                                                                                                       >  2:1139546/1‑288 (MQ=255)
                                                                                                                                        tGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTAt                                                                                                                                          >  2:710541/1‑286 (MQ=255)
                                                                                                                                           cGAATTTGCCGTGCTGCGCATCGCCAGGTTCCCGGCGAAAGGGTCGCTGCTGATAATGGATAACCGCATTAACCAGCATGTGGTCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTgg                                                                                                                                     <  2:324333/288‑1 (MQ=255)
                                                                                                                                                  gCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGGGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTgcgc                                                                                                                              >  1:1824711/1‑288 (MQ=255)
                                                                                                                                                       tCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTg                                                                                                                        <  1:615966/289‑1 (MQ=255)
                                                                                                                                                        cTGCGCATTCGCATGTTCCCGGCGGAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGc                                                                                                                                              >  2:672265/1‑266 (MQ=255)
                                                                                                                                                            gcATTCGCATGTTGCCGGCGAAAGGATGGCTACTGATCATGGGTGACCGCCTTAACACGCAGGTGATCAACCGTTGCCGTAACCCTTCCGACGCAGTTATTCTGGTGC‑CTGGCGATACCAGTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATGTTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGc                                                                                                                   <  2:867012/289‑1 (MQ=255)
                                                                                                                                                                tCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAACAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGGGGGCCTCTTGTGGCGTGCGCCTGCTGGTTGCCAGc                                                                                                               >  2:1156610/1‑289 (MQ=255)
                                                                                                                                                                    aTGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATg                                                                                                           <  1:112513/289‑1 (MQ=255)
                                                                                                                                                                            ggCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGCGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCTTGCGCCCGCTGGTTGCCCGCGAGGACGcccag                                                                                                   >  2:1250179/1‑287 (MQ=255)
                                                                                                                                                                               gAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAg                                                                                                 <  1:563306/288‑1 (MQ=255)
                                                                                                                                                                               gAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CGGGGGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGGGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAg                                                                                                 <  2:1873477/288‑1 (MQ=255)
                                                                                                                                                                               gAAAGGATCGCTGCGGATCGTGGATCACCGCATTAACCCGGATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAg                                                                                                 <  2:1382540/288‑1 (MQ=255)
                                                                                                                                                                                  aggatcgctgctgATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCgg                                                                                              >  1:731517/8‑288 (MQ=255)
                                                                                                                                                                                                  aTGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTg                                                                             <  1:466305/289‑1 (MQ=255)
                                                                                                                                                                                                    ggATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGc                                                                           >  2:794042/1‑289 (MQ=255)
                                                                                                                                                                                                    ggATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGGTGCCCGCGATGCCGACCCGAGCGGACGTAAGCTCAGCCggtg                                                                            >  2:55193/1‑285 (MQ=255)
                                                                                                                                                                                                    ggATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGACGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCCATGACGACCCGAGCGTACGTAACCTCAGCCCTgc                                                                            >  2:1298221/1‑287 (MQ=255)
                                                                                                                                                                                                        cACCGCATTAACCAGCATTTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCg                                                                       <  1:699559/289‑1 (MQ=255)
                                                                                                                                                                                                        cACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCg                                                                       <  1:291057/289‑1 (MQ=255)
                                                                                                                                                                                                         aCCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCg                                                                       <  2:1621869/288‑1 (MQ=255)
                                                                                                                                                                                                         aCCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCg                                                                       >  1:1621869/1‑288 (MQ=255)
                                                                                                                                                                                                                tAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGc                                                                                                                                              >  2:224929/1‑210 (MQ=255)
                                                                                                                                                                                                                tAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGc                                                                                                                                              <  1:224929/210‑1 (MQ=255)
                                                                                                                                                                                                                            gATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGa                                                    >  2:749084/1‑288 (MQ=255)
                                                                                                                                                                                                                                acccGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTGGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAAcgcg                                               <  1:122046/287‑1 (MQ=255)
                                                                                                                                                                                                                                aaCCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAAcgcg                                               <  1:982871/289‑1 (MQ=255)
                                                                                                                                                                                                                                aaCCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAAcgcg                                               <  1:1572127/289‑1 (MQ=255)
                                                                                                                                                                                                                                 aCCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAAAGTCAGCAGTGGCATCGTGCTGCTCGACCATGCTGAACGCGa                                              >  1:626944/1‑289 (MQ=255)
                                                                                                                                                                                                                                     cTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGCCGGAACGTCAGCCGTGTCATCGTGCTGCCCGACCATGCTGAAccgcatgg                                           >  2:53927/1‑281 (MQ=255)
                                                                                                                                                                                                                                     cTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGGGCTGCCGGACCCTGCTGAGCGCCATgg                                           >  2:809837/1‑288 (MQ=255)
                                                                                                                                                                                                                                      tgacGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTc                                         <  1:1674282/286‑1 (MQ=255)
                                                                                                                                                                                                                                          gTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGa                                     <  1:1950553/289‑1 (MQ=255)
                                                                                                                                                                                                                                            aaaCCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGAc                                    <  1:1288866/288‑1 (MQ=255)
                                                                                                                                                                                                                                               ccTTCCCACGCCGGTATTCTGGTGC‑CGGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGAt                                <  1:8418/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  tCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGt                                                                                                                                                                                                                >  1:1927311/1‑110 (MQ=255)
                                                                                                                                                                                                                                                  tCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGAc                                                            <  1:171362/258‑1 (MQ=255)
                                                                                                                                                                                                                                                  tCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTa                             >  1:1210463/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     tGC‑CGGGCGATACCATTTCGCTGATTGCTACCACCTCTTTACGTATTGATTACAACGGGATTGACGCTAATCGGGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTCAAGGGGAAAAACTGGCCCCGTTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGTCGTGCGCCCTCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCa           <  1:727391/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       c‑cTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCg                                                                       <  2:275791/226‑1 (MQ=255)
                                                                                                                                                                                                                                                                          tGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGa                                     >  1:932464/1‑258 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACtga     >  1:975587/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGAAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCAGCTCGCCCATGCTGAACGCGATGGTCTGGCCGGACTTATCACCATCACCGGTGGCAAAAtga     >  1:1544867/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             cGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACtgat    <  1:1920691/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                     
CTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGAAGCCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGC‑CTGGCGATACCATTAGCCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGA  >  CP042583/1924417‑1924974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A