Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 559,765 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583559,7650AT98.1% 626.9 / NA 269intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (143/120);  minor base G (1/4);  total (145/124)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583559,7660CG99.2% 636.6 / NA 269intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (144/122);  minor base T (1/1);  total (145/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583559,7670TA98.5% 629.9 / NA 269intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (144/120);  minor base G (0/4);  total (145/124)
Fisher's exact test for biased strand distribution p-value = 4.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑GCTCTCCCAGGCA‑TGGGCACTGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTGCTGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCACTGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGGCTCTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGAACTAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGGCAGCAGCTCCGGGATCACCAGATACCAGGTAAAGA  >  CP042583/559490‑560045
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
cccGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcg                                                                                                                                                                                                                                                                             >  2:1792261/1‑289 (MQ=255)
 ccGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg                                                                                                                                                                                                                                                                            <  1:1392099/289‑1 (MQ=255)
    tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGAc                                                                                                                                                                                                                                                                         >  1:261161/1‑289 (MQ=255)
    tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGAc                                                                                                                                                                                                                                                                         >  1:2563116/1‑289 (MQ=255)
    tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGAc                                                                                                                                                                                                                                                                         >  2:2920551/1‑289 (MQ=255)
              atGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGCAAAcgcgc                                                                                                                                                                                                                                                               >  2:1424549/1‑288 (MQ=255)
              atGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGa                                                                                                                                                                                                                                                               >  1:1719/1‑289 (MQ=255)
               tGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              <  2:907176/289‑1 (MQ=255)
               tGGCAGGAAAGGGACAATGGAAGTCCAGTCAAACTCGTACATTAGTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGGGGCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAC‑CGACCCCCAGGCA‑TGGGCTGAGTAATCGGGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              <  2:521900/289‑1 (MQ=255)
               agggagggaagggaCAAGGGAACTCCAGGCAAACTAGTACATTATTTGCCCCCCATGTGGACAAGGAAGCGAGCTTTCCGTTCAAACAGCGTCATCACCAGCAGGGTAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACACCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              <  2:3152774/285‑1 (MQ=255)
                  cAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGAATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              <  1:2105161/286‑1 (MQ=255)
                      gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCCTATCCACCAGACCGATAGTCGAGGCGATGGCCGAGGTTTTCACCCGGTTCATCATCCCTGAGGTCATCGGCGGGACAGTAACCCGATAAGAAtt                                                                                                                                                                                                                                                       >  1:1508139/1‑289 (MQ=255)
                      gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCAtt                                                                                                                                                                                                                                                       >  1:1066508/1‑289 (MQ=255)
                            aCAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAAACGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGCGGCGATGGCGGAGTTTTACACCAGGTTCATCATCCTCGAGGTCAACGGCGGGACGCTAAcccggacacaacatggac                                                                                                                                                                                                                                                  >  2:2105161/1‑274 (MQ=255)
                             cAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGgcag                                                                                                                                                                                                                                                 <  1:306320/288‑1 (MQ=255)
                                tGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGCCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGCTCATCATCTCTGAGGTCATCGGCGGGACGATAACCCGATAAGCATTAAGCAGCAGt                                                                                                                                                                                                                                             >  1:3011218/1‑289 (MQ=255)
                                 ggAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCCCGTAGGTCATCGGCGGGACGACAACCCGATACGCAATAGGCAGCAGTa                                                                                                                                                                                                                                            >  2:1132787/1‑289 (MQ=255)
                                    aCTCCAGACAAACTCGTACATTTTTTGCCCCCCATGTTGCCAGGCAGGGGAACTTTAAGTTCAACCAGCGTCATCACCAGGATAAAAAAAGCGTTATACAAGACATACGCCGACGTAAGGGCGGTAAC‑CGACTCCCGGGAA‑TGGGCTGAGTAATCGAGTAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGt                                                                                                                                                                                                                                         <  2:1586206/289‑1 (MQ=255)
                                     cTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAAAACGCGCTAAGCATAAGGCACCAGTACGTa                                                                                                                                                                                                                                        >  2:2423221/1‑289 (MQ=255)
                                            cAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGAt                                                                                                                                                                                                                                   <  2:1167809/287‑1 (MQ=255)
                                            aaacccctaaactaattTCCCCCCCAGTTTGCAAGGAAGCCGAACTTTCAGTCCAACCAGCGTAATCACAGGAATGATAAAAGCGTTAATCACCACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGAt                                                                                                                                                                                                                                   <  2:1909199/273‑1 (MQ=255)
                                                    tACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGGTAACGGCGTAAGCATTAGGCCGCAGTACGGATCGATAAGCCTGCgg                                                                                                                                                                                                                         >  2:3009551/1‑289 (MQ=255)
                                                    tACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCgg                                                                                                                                                                                                                         >  1:2491326/1‑289 (MQ=255)
                                                     atattattGGCCCCCCACGTGGCAGGGCAGGCGAACTTTACGTTAAACCAGGGTCATAACCAGCAGAAAAAACGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGCCTCCCAGGCA‑TGGGCTGAGTAATCGGGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGt                                                                                                                                                                                                                        <  2:305896/287‑1 (MQ=255)
                                                                    cATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACg                                                                                                                                                                                                                                          >  1:992411/1‑256 (MQ=255)
                                                                         tGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGc                                                                                                                                                                                                       <  1:1681247/286‑1 (MQ=255)
                                                                              ggcaggcGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATc                                                                                                                                                                                                                                      >  1:2278358/1‑250 (MQ=255)
                                                                              ggcaggcGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGcgcg                                                                                                                                                                                                >  1:1645203/1‑288 (MQ=255)
                                                                                    cGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCAtttt                                                                                                                                                                                         >  1:1453834/1‑289 (MQ=255)
                                                                                             cGTTCAACACGCGTCATCACCATACTGATAAAAGCGTTAATCAACACATACGCCGACGTAATGGCGGTAAA‑CGACTCCAAGGAA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTc                                                                                                                                                                                <  1:640128/289‑1 (MQ=255)
                                                                                                  aaacacggTCATCCACAGCATATTAAAGGCATTGATCAAACCAGACGCCGGAGTAAGGGGGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGAAATTTACCCGCTTGCGCCGCCTTATCCACCAGACCGGTAGTCGAGGCGCTGGCGGAGTTTTTCCCCAGGTTCATCATCTCTGGGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGc                                                                                                                                                                           <  2:1217904/282‑1 (MQ=255)
                                                                                                  aaCCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCTATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGc                                                                                                                                                                           >  1:377902/1‑289 (MQ=255)
                                                                                                   aCCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCa                                                                                                                                                                                                                 <  1:733388/250‑1 (MQ=255)
                                                                                                   aCCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCa                                                                                                                                                                                                                 >  2:733388/1‑250 (MQ=255)
                                                                                                            atcaCCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGCTAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCCTCAGGCCCATCGACAGCGCCGGATTTTTTTGCCCCCCCGGGAgcgcacgac                                                                                                                                                                 >  2:1836389/1‑281 (MQ=255)
                                                                                                               aCCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATcg                                                                                                                                                              <  1:3186021/289‑1 (MQ=255)
                                                                                                               aCCAGCATAATAAAACCGTTAATCAACCCATACGCCAGCGTAAGGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGGGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATcg                                                                                                                                                              <  1:1027729/289‑1 (MQ=255)
                                                                                                                 cAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATcgcg                                                                                                                                                            <  1:2423221/289‑1 (MQ=255)
                                                                                                                       gATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGTGCGt                                                                                                                                                      >  1:274826/1‑289 (MQ=255)
                                                                                                                         tAAAAGCGTGAGTCAACCAATACCACAGCGTAACGGGGGT‑AA‑GCGCCTCCAGGGACTGGGCTGAGTAACAGGGCATTTGCCCCGCCTGCGCCGCCAGATCCACCAGACCGATAGTCGAGGCGATGGCGTAGTTTTTCACCAGGTTAATCATCTCTGAGGTCATCGGCGGTACGATAAGGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGGGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTAc                                                                                                                                                    <  2:2580458/289‑1 (MQ=255)
                                                                                                                           aaaGCGTTAGTCAAAACAACCGCCAGCGTAATGGCGGTAAA‑CGCCCCCCGGGCA‑GGGGCTGAGAAATCCGGCAATTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCt                                                                                                                                                  <  2:1453834/289‑1 (MQ=255)
                                                                                                                                 ttAATCAACACTTACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGc                                                                                                                                            >  1:1346406/1‑289 (MQ=255)
                                                                                                                                 ttAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGt                                                                                                                                                                                                                                             >  2:2084002/1‑192 (MQ=255)
                                                                                                                                 ttAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGt                                                                                                                                                                                                                                             <  2:2282935/192‑1 (MQ=255)
                                                                                                                                  tAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCa                                                                                                                                                                                                                                                         >  2:1077147/1‑179 (MQ=255)
                                                                                                                                  tAATCAACACATACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCCCGCGGCAGCGACTTAATCGCGGCGCGTACCTGTTCGCa                                                                                                                                           >  1:1517426/1‑289 (MQ=255)
                                                                                                                                         cacaTACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCCTTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAacaccg                                                                                                                                    >  1:1891546/1‑287 (MQ=255)
                                                                                                                                          acttACGCCCGGGTAAGGGCGGTAAACGCAC‑CCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACggg                                                                                                                                   <  2:1346406/286‑1 (MQ=255)
                                                                                                                                            aTACGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATAGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGGTCGCAAACACCggcg                                                                                                                                 >  1:294870/1‑289 (MQ=255)
                                                                                                                                              aCGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATAGATAAGCCCGCGGTAGCGTCAGGCACATCGCCAGCGGGGCATTTTTTTGCCCCTGCGGAAGCGACTGAATCGCGGCGCGTAGCTGTTCGCAAACCCGggcggc                                                                                                                               >  1:2782569/1‑289 (MQ=255)
                                                                                                                                               cGCCAGCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGgcggcg                                                                                                                              >  1:1839574/1‑289 (MQ=255)
                                                                                                                                                    gCGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              >  2:2632319/1‑156 (MQ=255)
                                                                                                                                                     cGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGgca                                                                                                                                                                                                                                                  <  2:379703/167‑1 (MQ=255)
                                                                                                                                                     cGTAATGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTaaca                                                                                                                        >  1:2689533/1‑287 (MQ=255)
                                                                                                                                                          tGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGAt                                                                                                                                                                                                                                                              <  1:833694/150‑1 (MQ=255)
                                                                                                                                                          tGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGcc                                                                                                                    >  1:2054298/1‑288 (MQ=255)
                                                                                                                                                          tGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCCCGGCCGCGACTGAAACGCGGCCCGTACCTGTTCCCAAACAAGGGCCGCGGTAAACAGcc                                                                                                                    >  2:2833847/1‑288 (MQ=255)
                                                                                                                                                          tGGCGGTAAA‑CGACTCCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCAGCACTTTTTTGCCCACGCGGCCGCGACTGAATCGCGGCGCGTACCTGTTCGCCAACACGGGACGCGGTAAACAGcc                                                                                                                    >  1:2861660/1‑288 (MQ=255)
                                                                                                                                                                      gactcCCAGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCa                                                                                                        <  1:1291995/287‑1 (MQ=255)
                                                                                                                                                                             aGGCA‑TGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGAt                                                                                                                                                                                                                                   >  1:221580/1‑159 (MQ=255)
                                                                                                                                                                                    gggctgagTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGaa                                                                                           >  1:891320/8‑289 (MQ=255)
                                                                                                                                                                                     ggctgagTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCa                                                                                                                                                                                                                 >  1:1599182/7‑170 (MQ=255)
                                                                                                                                                                                     ggctgagTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGaaa                                                                                          >  1:2647543/7‑289 (MQ=255)
                                                                                                                                                                                         gagTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTAc                                                                                                                                                    >  1:2820903/3‑227 (MQ=255)
                                                                                                                                                                                          agTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTg                                                                                     >  1:54674/2‑289 (MQ=255)
                                                                                                                                                                                          agTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGGACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGGGGCAAGGAAAAACTg                                                                                     >  2:310854/2‑289 (MQ=255)
                                                                                                                                                                                          agTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGAGGTAGCGGCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCCGCGACTGGATCGCGGCGCGGTCCTGTTCGCAAACACGGGCGGCGGTAACCAGCCCCAGGCCGCGCCTGGACGAAAGGAAAAACTg                                                                                     >  2:1694356/2‑289 (MQ=255)
                                                                                                                                                                                           gTATTCGAGCAATTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTg                                                                                     <  1:1814293/288‑1 (MQ=255)
                                                                                                                                                                                                 gcgCAAGTTCCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTg                                                                              <  1:2358005/287‑1 (MQ=255)
                                                                                                                                                                                                 gAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAgg                                                                                                               >  1:816575/1‑256 (MQ=255)
                                                                                                                                                                                                    cAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATgg                                                                                                     >  1:1015923/1‑263 (MQ=255)
                                                                                                                                                                                                           cccGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCAACTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCt                                                                    <  2:738980/289‑1 (MQ=255)
                                                                                                                                                                                                           cccGCCTGCGCCGCCAGATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATTTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCt                                                                    <  1:2010166/289‑1 (MQ=255)
                                                                                                                                                                                                           ccagCCTGAGCCGCAATATCAACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCCCCAGGTTCATCAACTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCt                                                                    <  1:2080877/286‑1 (MQ=255)
                                                                                                                                                                                                            ccGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGAGGAAAGGAAAAACTGAAAATTGGGATCCAGCTc                                                                   >  1:186111/1‑289 (MQ=255)
                                                                                                                                                                                                            ccGCCTCGGCGGCCATATCCACCCGCCCGATGGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATAATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTc                                                                   <  2:1516676/289‑1 (MQ=255)
                                                                                                                                                                                                                 tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACggg                                                                                                                                   <  2:2029976/220‑1 (MQ=255)
                                                                                                                                                                                                                        ccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTa                                                                                                                                                                                                                                                      <  1:1149134/98‑1 (MQ=255)
                                                                                                                                                                                                                                     aCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCCGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACCGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGAGGAAAGGAAAAACTGAATATTGGGATCCCGACCGGCCTTAAACCACATGCCCAtttt                                           >  2:2593942/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATTGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGCTGAAAGGAAAAACt                                                                                      >  2:344302/1‑245 (MQ=255)
                                                                                                                                                                                                                                     aCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTaa                                                                                                                          >  1:1552146/1‑209 (MQ=255)
                                                                                                                                                                                                                                     aCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCCTGGAGGAAAGGAAAAACTGAATATTGGGATCCAGCCAGGCCTTAAACCAACTGCCCAttt                                            >  2:2803402/1‑287 (MQ=255)
                                                                                                                                                                                                                                     aCCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACt                                                                                      <  1:344302/245‑1 (MQ=255)
                                                                                                                                                                                                                                       cGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAgcga                                                                                                                                                                      >  1:1373367/1‑160 (MQ=255)
                                                                                                                                                                                                                                       cGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAgg                                                                                                               >  2:1316584/1‑218 (MQ=255)
                                                                                                                                                                                                                                       cGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAgg                                                                                                               <  1:1316584/218‑1 (MQ=255)
                                                                                                                                                                                                                                         atagtcgaggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCg                                      >  2:929780/9‑289 (MQ=255)
                                                                                                                                                                                                                                            gtcgaggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTc                                                                   >  1:1779678/6‑257 (MQ=255)
                                                                                                                                                                                                                                              cgggacGAGGGCGGAGTGTTTCCCCATGTTCATCATCGCTGGGGTCAGCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGGAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGgcag                                  <  2:54674/283‑1 (MQ=255)
                                                                                                                                                                                                                                              cgaggggATGGGGGAGTTTTTCACCAGGTTCAGCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGgcag                                  <  2:1676043/282‑1 (MQ=255)
                                                                                                                                                                                                                                               gaggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCA‑TTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTa                                                            >  1:1191299/3‑260 (MQ=255)
                                                                                                                                                                                                                                                aggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGatgaaagg                                                                                             <  1:437353/226‑8 (MQ=255)
                                                                                                                                                                                                                                                 ggCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCa                                                                                                             >  1:469159/1‑210 (MQ=255)
                                                                                                                                                                                                                                                      tGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCt                                                                    <  2:2494031/246‑1 (MQ=255)
                                                                                                                                                                                                                                                       ggCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAgg                                                                                                               <  2:2445335/202‑1 (MQ=255)
                                                                                                                                                                                                                                                       ggCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAgg                                                                                                               >  1:2445335/1‑202 (MQ=255)
                                                                                                                                                                                                                                                         cGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGGCAGCAGCTCCGGGa                       <  1:1132787/288‑1 (MQ=255)
                                                                                                                                                                                                                                                           gAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGt                                                                                                                                                                                                                                         <  2:458155/76‑1 (MQ=255)
                                                                                                                                                                                                                                                                             atcatcTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGGCAGCAGCTCCGGGATCACCAGATACCAGGTAAAGa  <  1:948600/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              tcatcTCTGAGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGCGACTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGATGAAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCCTAAACCACATGCCGGTTTTCTCCGGCCGCAGCCCCCGGATCACCAGATAc            >  2:2745126/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
CCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAA‑GCTCTCCCAGGCA‑TGGGCACTGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTGCTGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCACTGGTCATCGGCGGGACGATAACGCGATAAGCATTAGGCAGCAGTACGTATCGATAAGCCTGCGGTAGCGTCAGGCCCATCGCCAGCGCGGCATTTTTTTGCCCTCGCGGCAGGCTCTGAATCGCGGCGCGTACCTGTTCGCAAACACGGGCGGCGGTAAACAGCCCCAGGCAGAGCATGGAACTAAGGAAAAACTGAATATTGGGATCCAGCTCGGCCTTAAACCACATGCCGATTTTCTCCGGCAGCAGCTCCGGGATCACCAGATACCAGGTAAAGA  >  CP042583/559490‑560045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A