Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	699,596	3 bp→TCG	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	699,596	0	A	T	99.3%	642.9 / NA	281	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (122/154); minor base G (0/2); total (125/156)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
*	CP042583	699,597	0	G	C	99.3%	649.5 / NA	281	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (124/153); total (125/156)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	699,598	0	C	G	99.3%	651.6 / NA	281	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (2/0); new base G (123/156); total (125/156)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 281 total aligned reads displayed.

GGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATAGTCATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACAGC‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGC  >  CP042583/699322‑699877

|||

ggAACAAGAAGCGAAATTAGTTGCCGATCATCTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCgg                                                                                                                                                                                                                                                                               <  1:2867506/288‑1 (MQ=255)

             aaaTTAGTTGCCGGTCATTTAATCGCGGCAAACCGGGCAGGGCATAATTCACATGGTATTGGCAAGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGAGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATGGGCCGTATCGGTTACt                                                                                                                                                                                                                                                                 <  1:725915/289‑1 (MQ=255)

              aaTTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACt                                                                                                                                                                                                                                                                 <  1:430117/288‑1 (MQ=255)

              aaTTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACt                                                                                                                                                                                                                                                                 <  1:2895188/288‑1 (MQ=255)

                ttAGTTGCCGATCATTTAATCGCGGCAAACCTGGAAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTggg                                                                                                                                                                                                                                                              <  1:1955022/289‑1 (MQ=255)

                     ttcggatactttAATCGCGACAAACCGTGAAGGGCATGATTCACATGGTATTGGCATGATGCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTAACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                                                                                                                                                                                         <  2:2309187/280‑1 (MQ=255)

                     tGCCGCTCTTTTAACGGCGGAAAACCGGGACGGGCAGGATTCACAGGGTGTTAGCATGATCCCAATCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                                                                                                                                                                                         <  2:1802828/289‑1 (MQ=255)

                          aTCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAgtg                                                                                                                                                                                                                                                    >  1:557261/1‑289 (MQ=255)

                                  aTCCCGGCAAACCGGGAAGGGACTGATTCGACTGGTATTGGCATGATCCCAAGCTATCTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcgg                                                                                                                                                                                                                                            <  2:2074777/289‑1 (MQ=255)

                                       ggCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCCCGGTATTGCCGCCGTGGCGCCACATAACTCG‑CACCATATCGGCCGTATCGGTTACTGGGCGGGAACGTGTGCAGTCGCgggg                                                                                                                                                                                                                                       >  2:727031/1‑289 (MQ=255)

                                         cAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCAAATCGGCCGTATCGGTTACTGGGCGGCGCAGTGTGCAGCGGCGGGGtt                                                                                                                                                                                                                                     >  1:236078/1‑289 (MQ=255)

                                                      gCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         <  1:2603158/288‑1 (MQ=255)

                                                      gCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         <  1:2025642/288‑1 (MQ=255)

                                                                cATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGt                                                                                                                                                                                                                                                                           >  1:1112675/1‑228 (MQ=255)

                                                                     tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGCAAGCGTATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          <  1:1851690/288‑1 (MQ=255)

                                                                     tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          <  1:839662/288‑1 (MQ=255)

                                                                     gaTTGGCCTGATCCCCAGCTATGTACGCTCCGGGAGTCAGGGGAACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          <  2:830485/287‑1 (MQ=255)

                                                                        tGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATAAACCATCATGCCAAAACCGTTAAAGAGACGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGt                                                                                                                                                                                                      <  1:2537409/289‑1 (MQ=255)

                                                                         ggCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACt                                                                                                                                                                                                                                                                 >  1:2326101/1‑229 (MQ=255)

                                                                              gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCa                                                                                                                                                                                                >  1:228225/1‑289 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑catcat                                                                                                                                                                                                                                                                                    >  1:336012/1‑204 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTggg                                                                                                                                                                                                                                                              <  2:2916628/226‑1 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGGGCAGTTTGCAGCGGCGGGGTTTGGCCTCATCCACTTTGTTAGCGTGGGCGGTATTCCaa                                                                                                                                                                                               >  2:459936/1‑289 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         >  1:2496147/1‑263 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          >  1:778692/1‑278 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCaa                                                                                                                                                                                               >  1:1902553/1‑289 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCaa                                                                                                                                                                                               >  1:1525402/1‑289 (MQ=255)

                                                                               aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCaa                                                                                                                                                                                               >  1:383880/1‑289 (MQ=255)

                                                                                  ccAAGCTATATACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCACCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑gg                                                                                                                                                                                           <  1:157387/289‑1 (MQ=255)

                                                                                        tATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCGCATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTcgcg                                                                                                                                                                                      <  2:383880/288‑1 (MQ=255)

                                                                                          tGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGcc                                                                                                                                                                                                                                                                             <  2:892733/200‑1 (MQ=255)

                                                                                          tGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCg                                                                                                                                                                                   >  1:1831817/1‑289 (MQ=255)

                                                                                                 tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          <  1:3030079/260‑1 (MQ=255)

                                                                                                 tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACg                                                                                                                                                                            >  1:2149743/1‑289 (MQ=255)

                                                                                                 tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCCCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGGTCGCG‑CGTTcccc                                                                                                                                                                             >  2:1323264/1‑286 (MQ=255)

                                                                                                    tGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCCTTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCGGCACGGTATTGCCGCCGTGGCGCTACAT‑ACATCGCATCATCTCGCCCGTATCGGTTACTTGGCGGAGCAGCTTGCAGCGGCGGGGTTCGTCCCTATCCCCTTTGTTACCGTGGTCGGGATTCCCAT‑GGTTGGCCCCTTCCCCGGt                                                                                                                                                                          >  2:3128265/1‑288 (MQ=255)

                                                                                                    tGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcgg                                                                                                                                                                                                                                            <  2:2840343/223‑1 (MQ=255)

                                                                                                    tGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         <  2:3117395/242‑1 (MQ=255)

                                                                                                           aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTa                                                                                                                                                                                                                                                                   <  1:95401/193‑1 (MQ=255)

                                                                                                           aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                                                                                                                                                          >  1:2724027/1‑250 (MQ=255)

                                                                                                           aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGAACGCGCCTTTGGTCCGGTCGCGGCACATGAAGCGATGGCGCTGGGGACTGAGAAAGCGCATCACCACGGTCTTGCCGTCGTGGCGCTACATAACTCG‑CATCATAACGGCCGTATCGGGTACTGGGCCGCGGCGTGTGCAGCGGCGGGGGCTGGTTCCATCCCCTTTGTTTGCGGGGCAGGTGTTCCCAT‑TGTCGCTCCCGTCCCCggctcgca                                                                                                                                                                     >  2:2779552/1‑280 (MQ=255)

                                                                                                           aGGGGAACCTCAAAATTAACCACCAGGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGTTGGCGATCGGGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACa                                                                                                                                                                   <  2:2651786/288‑1 (MQ=255)

                                                                                                             gggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCTCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcggcg                                                                                                                                                                                                                                          >  1:666311/1‑216 (MQ=255)

                                                                                                             gggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGcc                                                                                                                                                                >  1:994530/1‑289 (MQ=255)

                                                                                                                      cAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGGATTCCAAT‑GGTCGCGCCGCTCCACGGTCTCGACAGCCGCCTTTGc                                                                                                                                                        >  2:302014/1‑288 (MQ=255)

                                                                                                                      cAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTCTGTCTCTACCCACTCTGGTGGCGTGGTCGGGATTCCAAT‑GGGCGCGCCGGTCCACGGGCCCGACACCCGCGTTGGc                                                                                                                                                        >  2:1854766/1‑288 (MQ=255)

                                                                                                                       aaaTTACCCATCATGCCAAAACGGTTAAAGGGGGGGGGGGGGCGGTCACGTTGGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCTAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       <  2:955310/288‑1 (MQ=255)

                                                                                                                       aaaTTAACCCACATGCCAAAACCGGTAAGGAGGCGGGGGCGGCGGTCACGCTGGAGGGCGAGCGCGGATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAGAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       <  2:2865522/288‑1 (MQ=255)

                                                                                                                       aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       <  2:1831817/288‑1 (MQ=255)

                                                                                                                       aaaTGAACCATGGTGCCCAAACCGGTAGGGGCGCGGGGGCGGCGTTCACGCTGGGTGGCGTTCTGGGGTTTGGTCAGGTCGCGGCGAGTGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGGGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       <  2:66689/288‑1 (MQ=255)

                                                                                                                        aaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       <  2:1543355/287‑1 (MQ=255)

                                                                                                                             aCCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAgcggcg                                                                                                                                                                                                                                          <  1:2217360/200‑1 (MQ=255)

                                                                                                                                     gCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTa                                                                                                                                                                                                                                                                   <  1:122795/167‑1 (MQ=255)

                                                                                                                                     gCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTa                                                                                                                                                                                                                                                                   >  2:122795/1‑167 (MQ=255)

                                                                                                                                     gCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCCCGGGCGCGACAGCGGCTTTGGCACCAACCCGTGTtgtg                                                                                                                                        >  1:2944762/1‑289 (MQ=255)

                                                                                                                                     gCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTgg                                                                                                                                                         <  1:2192040/272‑1 (MQ=255)

                                                                                                                                         aaaCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                                                                                                                                            >  2:2178337/1‑170 (MQ=255)

                                                                                                                                          aaCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGGCTCCAACCACTTTGTTAGCGTGGTCGGTATTTCa                                                                                                                                                                                                >  2:2987370/1‑229 (MQ=255)

                                                                                                                                            ccGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGtttt                                                                                                                                 <  2:643163/289‑1 (MQ=255)

                                                                                                                                              gTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt                                                                                                                                                  >  1:1987765/1‑270 (MQ=255)

                                                                                                                                              gTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt                                                                                                                                                  <  2:1987765/270‑1 (MQ=255)

                                                                                                                                                       gCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                                                                                                                                                                                         <  1:3126367/159‑1 (MQ=255)

                                                                                                                                                       gCGGGGGCGGCGATCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTTAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTTCTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTTTCCACCTTGTTTGCGTGTTCGGGTTTTCCAT‑GGTCTGGCCGTTCCACCGTCTCACCGACCGCTTTGGCAACACATCGTTCTGTTTTGTTTTCCCTCGTGAAg                                                                                                                      >  2:2020381/1‑289 (MQ=255)

                                                                                                                                                                     cACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCAGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCt                                                                                                         <  1:1356862/288‑1 (MQ=255)

                                                                                                                                                                        gCTCGATGGGGACGGCGCATTTGGGCAGGTCGCGGCAACTGAAGCGATGGCGCTGGGAATTGAGAATGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTg                                                                                                     <  2:2228334/289‑1 (MQ=255)

                                                                                                                                                                        gCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTg                                                                                                     <  1:460092/289‑1 (MQ=255)

                                                                                                                                                                         gtCGATGGCGATCGGGCAGTTGGGCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTg                                                                                                     <  2:2383786/287‑1 (MQ=255)

                                                                                                                                                                           cGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGAGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTcgcg                                                                                                                                                                                      >  2:1409688/1‑205 (MQ=255)

                                                                                                                                                                             aTGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         <  2:2796944/169‑1 (MQ=255)

                                                                                                                                                                             aTGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         >  1:2796944/1‑169 (MQ=255)

                                                                                                                                                                                  gATCGGGCATTTGGTCGGGTCGCGGCACAGGAGGCGGTGGCGGTGGGGATTGAGAAGGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTAc                                                                                            <  2:1912782/288‑1 (MQ=255)

                                                                                                                                                                                       cgcATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGccacc                                                                                      >  1:2844685/1‑289 (MQ=255)

                                                                                                                                                                                          aTTTGGACAGGTGGCGGCCAGTGAGGCGAGGGGGCTGGGGATTGGCAAAGCGCATCAGCACGGTATTGCCGCCGGGGCGCTACATACCTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAg                                                                                    <  2:2532892/288‑1 (MQ=255)

                                                                                                                                                                                              ggTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCa                                                                               <  1:2387236/289‑1 (MQ=255)

                                                                                                                                                                                                     tCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCAt                                                                         <  1:1816728/288‑1 (MQ=255)

                                                                                                                                                                                                              aTGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                                                                                                                                            >  1:2417851/1‑101 (MQ=255)

                                                                                                                                                                                                              aTGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                                                                                                                                            <  2:2417851/101‑1 (MQ=255)

                                                                                                                                                                                                               tGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCCATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTgg                                                                     >  1:1923840/1‑282 (MQ=255)

                                                                                                                                                                                                                    cGATGGCGCTGGGTATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCcgcg                                                          <  1:676643/288‑1 (MQ=255)

                                                                                                                                                                                                                       tGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTggg                                                                                                                                                                                                                                                              <  1:2097766/90‑1 (MQ=255)

                                                                                                                                                                                                                         gcgtTGGGGATTGCGAAAGCGCATCAGCACGGTATGTCCGACGTGGCGCTACATAACTGG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGGGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGcc                                                     <  2:1343602/288‑1 (MQ=255)

                                                                                                                                                                                                                         gcgcTGGGGATTGAGAAAGCGCATCAGCACGGTCTTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGcc                                                     <  1:1775236/288‑1 (MQ=255)

                                                                                                                                                                                                                          cgcTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTg                                                   >  1:1509729/1‑289 (MQ=255)

                                                                                                                                                                                                                            cTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCgg                                                                                                                                                                                                       >  2:1608117/1‑140 (MQ=255)

                                                                                                                                                                                                                            cTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTg                                                                                                 >  1:1015091/1‑241 (MQ=255)

                                                                                                                                                                                                                                    tGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt                                                                                                                                                  <  1:2683836/184‑1 (MQ=255)

                                                                                                                                                                                                                                    tGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGc                                                                                                          <  1:1129954/224‑1 (MQ=255)

                                                                                                                                                                                                                                           gcgcATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCAt                                                                         <  2:573597/250‑1 (MQ=255)

                                                                                                                                                                                                                                                     aCGGTATGGCCGCCGTGGCGCTACATAACTGG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAg                         <  1:1475888/288‑1 (MQ=255)

                                                                                                                                                                                                                                                      cGGTATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                                                                                                                                                       >  2:1960148/1‑161 (MQ=255)

                                                                                                                                                                                                                                                         tATTGCCGCCGTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTg                     >  2:2397358/1‑288 (MQ=255)

                                                                                                                                                                                                                                                          aTTGCCGCCGTGGCGCTACATAACTCG‑CATCATATGGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGcc                   <  1:3002452/289‑1 (MQ=255)

                                                                                                                                                                                                                                                              ccgccgTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCt                                                                                                         <  2:1135352/199‑1 (MQ=255)

                                                                                                                                                                                                                                                              ccgccgTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAt               >  1:3080776/1‑289 (MQ=255)

                                                                                                                                                                                                                                                               cgccgTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAtt              <  1:2397358/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                 ccgTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTcc                                     >  2:1515066/1‑264 (MQ=255)

                                                                                                                                                                                                                                                                   gTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                                                                                                                                                         <  2:1929079/83‑1 (MQ=255)

                                                                                                                                                                                                                                                                   gTGGCGCTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCCTCGCCTGGCATAAAGGCGGCCCCGTGCCGCCAGGTTGCCCGATTGACg          >  2:104760/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                       cgcTACATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAtt              <  1:640471/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                           aCATAACTCG‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTGTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGc  <  2:2149743/289‑1 (MQ=255)

|||

GGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATAGTCATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACAGC‑CATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAAT‑GGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGC  >  CP042583/699322‑699877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A