Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,925,070 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,925,0700AT99.7% 702.9 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (133/159);  minor base G (1/0);  total (134/159)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,925,0710GC100.0% 701.7 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (134/159);  total (134/159)
*CP0425831,925,0720TA99.7% 698.3 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (133/159);  minor base C (1/0);  total (134/159)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

AACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTAGTCAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGCCGCGCTGCCGGA  >  CP042583/1924795‑1925348
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                     
aaCTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGc                                                                                                                                                                                                                                                                            <  2:2246374/289‑1 (MQ=255)
          cGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTAcc                                                                                                                                                                                                                                                                  <  1:899751/289‑1 (MQ=255)
          cGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTAcc                                                                                                                                                                                                                                                                  <  1:1267521/289‑1 (MQ=255)
                     aaaCGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAg                                                                                                                                                                                                                                                        >  1:3075658/1‑288 (MQ=255)
                      aaCGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTc                                                                                                                                                                                                                                                      <  1:1634191/289‑1 (MQ=255)
                           gcATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑tct                                                                                                                                                                                                                                                 >  1:2362265/1‑288 (MQ=255)
                                    gggCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCcctgcctg                                                                                                                                                                                                                                        <  1:623375/288‑1 (MQ=255)
                                             cTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTgcg                                                                                                                                                                                                                              <  1:744352/289‑1 (MQ=255)
                                                gCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTCCCTGGTGCACAAAAACCCGATGACGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCCCTGCGCgg                                                                                                                                                                                                                           >  2:741002/1‑289 (MQ=255)
                                                     cgcCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTCTCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAAAACGCGCCCACGTACGACTGCCGATATGGCACTGCCTGGTTCACAAGAACCCGCTGAAGt                                                                                                                                                                                                                                                                      >  2:2358047/1‑242 (MQ=255)
                                                     cgcCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCCGGTTCCCCAGAACCCGCTGAAGTTACCTTGCGGAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCCCGGTTcc                                                                                                                                                                                                                       >  2:865107/1‑287 (MQ=255)
                                                     cgcCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCCGGCACGTCCTGGTTCACAAGAACCCGCCGAAGTTACCTTGCGGAAAGGCATTCCCCCTGCCTCCCCGCCTGCG‑GGTTCt                                                                                                                                                                                                                       >  2:1713970/1‑288 (MQ=255)
                                                     cgcCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGGATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTTCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCC‑TCTCCCTCCCTCCCCCTCTGCGCCGCTCTg                                                                                                                                                                                                                      >  2:2697227/1‑289 (MQ=255)
                                                      gcCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTg                                                                                                                                                                                                                      <  1:1996966/288‑1 (MQ=255)
                                                               ttGCCCGCGACGACGACACGGGCGGCGGTACGGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATc                                                                                                                                                                                                             <  2:1007428/288‑1 (MQ=255)
                                                                       gttGACGCCCGGGGGGGACGTAAGGGCAGCCGTGGCATCGTGCTGCCCGACAATGCTGAACGCGATGGGCTGGACGGATTTATCACCACCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTCCCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGc                                                                                                                                                                                                     <  2:2338167/286‑1 (MQ=255)
                                                                            cggcccGGGCGGACGTAAGGGCAGCCGTGGCATCGGGCTGCTCGACCAGGCTGGACGGAGTGGTCGGGAGGGATTTATCACCATAACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGGTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCg                                                                                                                                                                                                <  2:2073079/285‑1 (MQ=255)
                                                                               cccGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCAc                                                                                                                                                                                             >  1:1311638/1‑288 (MQ=255)
                                                                                  gacgggACGTAACGTCCGCCGGGGCGTCGTGCTGCCCGACCAGGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGAGGACCTTTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACgc                                                                                                                                                                                           <  2:1044576/283‑1 (MQ=255)
                                                                                     tggagttAACGTCAGCCGTGGGATCGTGCGGCTCGACCAGGCTGAGCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACTCGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACgcctgc                                                                                                                                                                                       <  2:1848676/282‑1 (MQ=255)
                                                                                              cGTCAGCCGTGTCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑tctc                                                                                                                                                                                                                                                <  1:1428167/222‑1 (MQ=255)
                                                                                                 cAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGaa                                                                                                                                                                          >  1:1598616/1‑289 (MQ=255)
                                                                                                  aGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGc                                                                                                                                                                                                                                                                            >  1:2947867/1‑191 (MQ=255)
                                                                                                  aGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAg                                                                                                                                                                         >  1:1761635/1‑289 (MQ=255)
                                                                                                    ccGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTAcc                                                                                                                                                                                                                                                                  >  2:3008522/1‑199 (MQ=255)
                                                                                                       tGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACGAGAACCCGCTGAAGTTACCTTCCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCCGCGGCTTATCGTCATTGCCATCGGACGCCTGCCTGGCTGAGCGAAGGCCg                                                                                                                                                                     >  2:1592275/1‑288 (MQ=255)
                                                                                                          cATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCt                                                                                                                                                                  <  1:865107/288‑1 (MQ=255)
                                                                                                                  tgctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         <  2:1833220/289‑1 (MQ=255)
                                                                                                                  tcggcgacgaTGCTGAACGGGATGGGCTGGACGGATTAAGCGCCATCACCGGGGGCAAACTGATGACCTATCGGCTGAGGGCTGAATGGGCTCCAGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAGGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         <  2:281808/285‑1 (MQ=255)
                                                                                                                    ctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCg                                                                                                                                                                                                >  1:822814/1‑248 (MQ=255)
                                                                                                                    ctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCg                                                                                                                                                                                                <  2:822814/248‑1 (MQ=255)
                                                                                                                    ctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAg                                                                                                                                                                         >  1:2235460/1‑271 (MQ=255)
                                                                                                                         ccATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCTAACTGATGACCTTTCGTCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGTGCAACTCGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGGAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCTTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGcc                                                                                                                                                       <  1:1184342/284‑1 (MQ=255)
                                                                                                                         ccATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGAt                                                                                                                                                                                                  <  2:1632573/241‑1 (MQ=255)
                                                                                                                         ccATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTa                                                                                                                                                  >  1:2799552/1‑289 (MQ=255)
                                                                                                                         ccATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGCCTGATGGCTGAATGTGCTACCGACGCGGTGTGCCTCAAACTGGTCAACACACGCTCCTGTACGACTGCCGATCTGGCACTTCCTGCTTCACCAGAACCCTCTGAAGTTACCTTTCGTAAAGTCA‑TCTCCCTGCCCGCCCCGCTTCGTGATTCCGCGTGTTATCTTCATTCCGACCGCCCGCCTGCCCGGCTTGTCGAAGTCCGCTGGCACCGTAGCCTggca                                                                                                                                                  >  2:1907137/1‑287 (MQ=255)
                                                                                                                                     ggATGGTCTGGACGGATTTTCCACCAGCCCCGGTGGCAAACTGATGACCTACCGGCTGATGGCGGACTGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGCCTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGa                                                                                                                                       <  2:2734836/287‑1 (MQ=255)
                                                                                                                                      gATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGc                                                                                                                                                                                  >  2:2042722/1‑244 (MQ=255)
                                                                                                                                      gATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGc                                                                                                                                                                                  <  1:2042722/244‑1 (MQ=255)
                                                                                                                                                   ggtttATCACCGACACCTGGTGCAAGCTGCTGACCTATCGGCTGAGGCCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCACCACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGACCCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCgg                                                                                                                         <  2:2194269/286‑1 (MQ=255)
                                                                                                                                                    aTTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGt                                                                                                                       >  1:947929/1‑289 (MQ=255)
                                                                                                                                                     tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGt                                                                                                                                                           >  1:567927/1‑252 (MQ=255)
                                                                                                                                                     tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTg                                                                                                                      <  1:584923/289‑1 (MQ=255)
                                                                                                                                                     tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTg                                                                                                                      <  1:3158918/289‑1 (MQ=255)
                                                                                                                                                       tATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGaa                                                                                                                    <  1:2662544/289‑1 (MQ=255)
                                                                                                                                                          caccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTg                                                                                                                 <  1:2322733/289‑1 (MQ=255)
                                                                                                                                                              atcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCa                                                                                                               >  1:2194269/1‑287 (MQ=255)
                                                                                                                                                              atcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGt                                                                                                             >  1:2831540/1‑289 (MQ=255)
                                                                                                                                                                caccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGCAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCCCACGCCTGCCTTGCGGAGCGAAGGCCGTCTGCACCGTATCCTGGTTTTTGAGTGCTAACCGTGAACTGTGGGTGaatgccagtcc                                                                                                           >  2:2658016/1‑280 (MQ=255)
                                                                                                                                                                       ggCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTa                                                                                                                                                                                                               <  1:2116988/182‑1 (MQ=255)
                                                                                                                                                                         cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAg                                                                                                                   <  2:2777036/272‑1 (MQ=255)
                                                                                                                                                                         cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGaa                                                                                                  >  2:57040/1‑289 (MQ=255)
                                                                                                                                                                           aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGc                                                                                                                                                                                                                                                                            >  1:1008378/1‑118 (MQ=255)
                                                                                                                                                                                atgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTAcc                                                                                                                                                                                                                                                                  <  2:677480/123‑1 (MQ=255)
                                                                                                                                                                                    ccgaTCGGGTGGTGGCTGAGTGGGCTACCAGCGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTa                                                                                            <  2:1946561/281‑1 (MQ=255)
                                                                                                                                                                                        tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGcc                                                                                                                                                       <  1:3087460/221‑1 (MQ=255)
                                                                                                                                                                                        tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTa                                                                                            >  1:1916431/1‑280 (MQ=255)
                                                                                                                                                                                        tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAAt                                                                                   >  2:195917/1‑289 (MQ=255)
                                                                                                                                                                                        tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAAt                                                                                   >  1:429516/1‑289 (MQ=255)
                                                                                                                                                                                         cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATa                                                                                  <  2:3083142/289‑1 (MQ=255)
                                                                                                                                                                                            cggATGGCTGAATGGCCTACCGACGCGTTATGCGGCAAGCTGGGCAACACGCGCCCCTGTACGACGGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGc                                                                                <  2:2831540/286‑1 (MQ=255)
                                                                                                                                                                                               aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACCCGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGa                                                                                                                                       <  1:2853495/230‑1 (MQ=255)
                                                                                                                                                                                               aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGc                                                                                                                                                                                                     <  1:668682/168‑1 (MQ=255)
                                                                                                                                                                                               aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGc                                                                                                                                                                                                     <  1:1686265/168‑1 (MQ=255)
                                                                                                                                                                                                      aaTGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCCCACGCCTGCCTGGCTGCGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGGAGCGGTCACTCCGGGTGACGGGCAGTACGCGGCAGAAAATTTAAACGTTAATAGACTGCCggtgtt                                                                     >  2:1201498/1‑285 (MQ=255)
                                                                                                                                                                                                          ggCTACCGCGGCGGTATGCCCCAACCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCCCTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCGCCCTGCCTGCCCCGCTGCGCGGTGCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACg                                                                  <  2:1159995/288‑1 (MQ=255)
                                                                                                                                                                                                           ggtACCGGCGCGGTATGCCGCAACCTGGGCAACACGAGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGc                                                                 <  2:2549943/286‑1 (MQ=255)
                                                                                                                                                                                                           gctgctgACGGGGTAGGCCGCAAACTGGGCAGCCCGCGCCCCGGTACGACGCCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGc                                                                 <  2:1761635/282‑1 (MQ=255)
                                                                                                                                                                                                                  aCGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGc                                                                 <  1:2732553/281‑1 (MQ=255)
                                                                                                                                                                                                                   cgcgGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTAc                                                         <  1:1043570/288‑1 (MQ=255)
                                                                                                                                                                                                                     cgGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGCGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCg                                                      >  2:3053510/1‑289 (MQ=255)
                                                                                                                                                                                                                             cGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGa                                              <  1:3126869/289‑1 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGt                                                                                                                                           <  2:1311342/191‑1 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCcg                                                               >  2:2565895/1‑267 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCcg                                                               <  1:2565895/267‑1 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGc                                         >  1:1565965/1‑289 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGc                                         >  1:577540/1‑289 (MQ=255)
                                                                                                                                                                                                                                  aCTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTACCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGc                                         >  1:2430462/1‑289 (MQ=255)
                                                                                                                                                                                                                                      ggCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         >  1:1299402/1‑173 (MQ=255)
                                                                                                                                                                                                                                           cacGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTAc                                                         <  1:1544411/264‑1 (MQ=255)
                                                                                                                                                                                                                                            acGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTAc                                                         <  1:195677/263‑1 (MQ=255)
                                                                                                                                                                                                                                                gcCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGa                           >  2:971325/1‑289 (MQ=255)
                                                                                                                                                                                                                                                  gccggttctaCTGCCGATCTGGCACTGCCGGGTTCACCAGACGCGGCTGAGGTGACAGTGCGTAGAGTCC‑TCTGCGTGCCACCACCGGTGCGCGGTTCTGCGGTGTATCGTCATGGGGAGCGAACGCCTGACGGGCTGCGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCATTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTGCCCGTGTGGGGATGGGCACCTGCCAGGGCGAAc                         <  2:2893955/280‑1 (MQ=255)
                                                                                                                                                                                                                                                     tGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         <  2:1453193/158‑1 (MQ=255)
                                                                                                                                                                                                                                                     tGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         >  1:1453193/1‑158 (MQ=255)
                                                                                                                                                                                                                                                     tGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGCTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTACCTGCGGGTGAAGTGCAGTACGCGGGAGAAAATTTAAACGTTAATAGCCTGCTTGATTTACTCCGTTGTACCCGTCTgggg                                               >  2:1544411/1‑264 (MQ=255)
                                                                                                                                                                                                                                                          gACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGc                                                                                                                           >  1:2490849/1‑183 (MQ=255)
                                                                                                                                                                                                                                                          gACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGc                                                                                                                           <  2:2490849/183‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTGTCGATCGGGCACTGCCTGGTTCACACGAACCCGCTGAAGTTACCTTTCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTGACCGTCATGGAGATCGCACGCCTGCCTGGCTGTGCGCAGGACGTCTGCACCGTAGCCTGGTATCTGAGTGCGAAGCCGTAACTGCGGTTCAAGTCCGGCACGCGGTAGAAAATTAAAACGTTAATACCCTGCTGGATTTACGCCGTCGTACACGTGTGGGGATGGGCACCTGCCGGGGCGAAct                        >  2:437052/1‑280 (MQ=255)
                                                                                                                                                                                                                                                            cTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAgg                                                                                                                                                                        <  1:185789/136‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCAcc                                                                                                                                                             <  1:3020243/147‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAAc                                                                                                                              >  1:836683/1‑178 (MQ=255)
                                                                                                                                                                                                                                                            cTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCcgtcg                                                            <  2:2429431/244‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTATGGGGATGGGCACCTTCAAGGGCGAACTCTCCGCCt                >  2:1280910/1‑288 (MQ=255)
                                                                                                                                                                                                                                                              gCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGt                                                                                                                                                           <  2:622389/147‑1 (MQ=255)
                                                                                                                                                                                                                                                              gCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGcc             >  1:1765877/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               ccGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGcc                                                                                                                                                       <  1:1117052/150‑1 (MQ=255)
                                                                                                                                                                                                                                                               ccGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGcc             >  2:1109999/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                      ggCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGc                                                                                                                                         >  2:3132462/1‑157 (MQ=255)
                                                                                                                                                                                                                                                                         aCTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAg                                                                                                                                                         <  1:1839832/138‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                     
AACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTAGTCAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCA‑TCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTAATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGCCGCGCTGCCGGA  >  CP042583/1924795‑1925348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A