Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 606,988 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583606,9880AT95.7% 416.8 / NA 187intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (64/114);  minor base C (8/0);  total (73/114)
Fisher's exact test for biased strand distribution p-value = 3.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583606,9890GC99.5% 433.9 / NA 187intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (72/113);  minor base T (1/0);  total (73/114)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583606,9900TA97.9% 430.4 / NA 187intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (70/113);  minor base C (3/1);  total (73/114)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

GGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCAGTCTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGAGCGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTT  >  CP042583/606709‑607262
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                
    gtgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGtt                                                                                                                                                                                                                                                                       >  1:1310737/1‑289 (MQ=255)
            ggACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCg                                                                                                                                                                                                                                                                >  1:110865/1‑288 (MQ=255)
            ggACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATAACTCACTCAGCCTGTTTGATCCgg                                                                                                                                                                                                                                                               >  1:871319/1‑289 (MQ=255)
               ccGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTTGCTGGCCGGTGATTGGTCATACCCCCCTCCGCCTTTTTGATCCGgccg                                                                                                                                                                                                                                                            >  2:1015832/1‑287 (MQ=255)
               ccGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGgcg                                                                                                                                                                                                                                                             <  1:1950896/288‑1 (MQ=255)
                         cGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                   <  2:989365/288‑1 (MQ=255)
                         cGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                   <  2:952337/288‑1 (MQ=255)
                            tggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCCGACTGGTGTTTATCCGCGGGCAACGCCGGCTGGCTGGCCGTTGTTTTGTCATACTCTCCTCCGCCTGTTTCATCCGGCGTGTGACGAccccg                                                                                                                                                                                                                                                >  2:430742/1‑287 (MQ=255)
                            tggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCa                                                                                                                                                                                                                                                >  2:600327/1‑288 (MQ=255)
                             ggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTTTTTATCCGCTTGCAACGCCTGGTTGCTGGCAGTTGATGTGATATACCTCACTCAGCCGGTTTGCGCCGGcgcggtctgaaccca                                                                                                                                                                                                                                                >  2:1619046/1‑276 (MQ=255)
                             ggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGATTTCAACAGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACACCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCCGT‑CGGGATCGGCGGGACGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCGGGCAGTTGATTGGTCATACCTCACTCCGCCTGTTTGATCCGGCGCGTGACGATCCCa                                                                                                                                                                                                                                                >  1:1273030/1‑287 (MQ=255)
                                 gcggcgTATTGCGGGTGGAGTGGAAGAAGGGTGGTTGAGTTCAAAGCCTACGTGGAATACGTGGTCTGGTTTTTATGTTTTCCACCGTGCTTCCCGTATCTCGGGCGTTTGCCGGACCCACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCttat                                                                                                                                                                                                                                          <  2:90615/289‑1 (MQ=255)
                                   ggcgCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTGTCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCttatt                                                                                                                                                                                                                                         <  1:1881666/288‑1 (MQ=255)
                                     cgcATTGCGGGATTAGCGAAAAACCGGTTGTTGAATTGCACTCCTCGGTGTAGTACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTAc                                                                                                                                                                                                                                       <  1:1092766/288‑1 (MQ=255)
                                      gcATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTGTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGt                                                                                                                                                                                                                                     <  1:991818/289‑1 (MQ=255)
                                              ggTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGgag                                                                                                                                                                                                                              <  1:110628/288‑1 (MQ=255)
                                                     cGAAAAGCAGGTTGTTGATTTGGCCTCCTCCGTGGAATAGGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGt                                                                                                                                                                                                                       <  2:1480083/288‑1 (MQ=255)
                                                     cGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGt                                                                                                                                                                                                                       <  2:274367/288‑1 (MQ=255)
                                                      gAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGt                                                                                                                                                                                                                       <  1:595960/287‑1 (MQ=255)
                                                                ttgttgAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTg                                                                                                                                                                                                               <  1:1015832/285‑1 (MQ=255)
                                                                 tgttgAATTGGACGCCTCCGTGGAATGCGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGGGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCg                                                                                                                                                                                                          <  2:243269/289‑1 (MQ=255)
                                                                 ggttgATTTGCACGCCTCCGTGGAATAGTTGGTTTGGTTTTTAGGTTTTCACCCGGGGTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCg                                                                                                                                                                                                          <  2:1959152/288‑1 (MQ=255)
                                                                        ttGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAagg                                                                                                                                                                                                   <  1:1771758/289‑1 (MQ=255)
                                                                           cACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAaggagg                                                                                                                                                                                                >  1:811536/1‑289 (MQ=255)
                                                                           cACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCCCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTTGACCGGAGAaggggg                                                                                                                                                                                                >  2:1002811/1‑289 (MQ=255)
                                                                            aCTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAaggagg                                                                                                                                                                                                <  1:112338/288‑1 (MQ=255)
                                                                             gtccgccGTGGAATACGTGGTTTGGTTTTCAGCTTGTCAACCGGGCTTCCCGTATGTCGGGGGTTTGCCGGGACCACTACACTCGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAggaggga                                                                                                                                                                                              <  2:1590100/288‑1 (MQ=255)
                                                                                ctccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTa                                                                                                                                                                                           >  1:364146/1‑289 (MQ=255)
                                                                                 tccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCTGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCCGTTTCATCCGGCGCGTGACGCAGCCATCTTATTACGTCCGGGAGACAGCTCTCGCTTTTTACCGCGGGAggaggggttgt                                                                                                                                                                                          >  2:1782634/1‑284 (MQ=255)
                                                                                   cGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGCGCGCTTTGTACCGCAGAAGGAGGGAGTAt                                                                                                                                                                                          >  1:289899/1‑287 (MQ=255)
                                                                                     tGGAATACGTGGTCTGGTTTTTAGGTTGTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCt                                                                                                                                                                                       <  1:1342879/288‑1 (MQ=255)
                                                                                          tACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGCCTGGTTTTTATTCGCTGGCCACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGGCTCGTTGATCTGGCGTGTGACGCACCCATCCTCTCACGTCCGGGCGCACGCTCTCGCTGTGTCCCGCAGTAGACGGGAGTATGCTGAAgct                                                                                                                                                                                 >  2:51793/1‑287 (MQ=255)
                                                                                          tACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                   >  1:24631/1‑223 (MQ=255)
                                                                                            cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCCACGCCGGGCGGCTGGCAGTTGATTGGTCATACCTCACCCCGCCTGTTTTCTCCGGGCCGTGACCAACCCATCTTATTCCGTCCGGGGGGCCGCGGTCGCTTTGTACCGCCGAAGGAGGGGTTAGGCTGAAGagta                                                                                                                                                                               >  2:1693239/1‑289 (MQ=255)
                                                                                            cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGatta                                                                                                                                                                               >  2:1807469/1‑289 (MQ=255)
                                                                                             gTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAagg                                                                                                                                                                                                   <  1:701408/268‑1 (MQ=255)
                                                                                               ggTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGCCGAACCCATCTTATTACGTCCTGGCGACAGCGTGCGCCTTGTACCGCAGAATGAGGGAGTATGCTGAAGATTTTTc                                                                                                                                                                            >  2:1390672/1‑289 (MQ=255)
                                                                                                  cTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGc                                                                                                                                                                                        <  2:1671950/274‑1 (MQ=255)
                                                                                                  cTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGc                                                                                                                                                                                        >  1:1671950/1‑274 (MQ=255)
                                                                                                  cTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTg                                                                                                                                                                         <  1:1637000/289‑1 (MQ=255)
                                                                                                      tttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCgg                                                                                                                                                                      <  1:1685422/288‑1 (MQ=255)
                                                                                                      tttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCgg                                                                                                                                                                      <  1:938927/288‑1 (MQ=255)
                                                                                                       ttttGGGTCTTCAACCGGGCTTCCGCTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:605753/289‑1 (MQ=255)
                                                                                                       ttttAGTGTTTCAACCGGGCTTCCCGTATCGCGGGAGTGTTCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTTGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:645069/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTGACGCGGGCTGCCCGGATCTCGGGAGTTTGCCGGAAACACGACACACGCCACGGCGCGCTGAACGGCGCTTACTCGTTCCGGCGGGTTCTGT‑CGGGAGCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:752652/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTCTCGCGGGAGGTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:1434062/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  1:672975/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:694496/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  1:1807469/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  1:1521083/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:110865/289‑1 (MQ=255)
                                                                                                       ttttAGGTGTTCAACCGGGATTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  1:1140287/289‑1 (MQ=255)
                                                                                                       ttttAGCTTTTCACCCGGGCTTCCCGTCGCTCGGGGGTGTGCCGGAACAACTCCACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:737456/289‑1 (MQ=255)
                                                                                                       gtttAGGTTTGCATCCGGGCTGCCCGGAGCTCGGGAGCTGGCCGAAACAACAACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTGCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:1949717/288‑1 (MQ=255)
                                                                                                          tAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGCACCCATCTTATTACGTCCGGGGTACAGCGGTCGCCTTTGACCGCAGCAGGGGGGAGGATGGCGGAGATTATTctgtcgtg                                                                                                                                                                     >  2:475682/1‑278 (MQ=255)
                                                                                                                ttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:1329549/280‑1 (MQ=255)
                                                                                                                 tCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                          <  1:1853810/289‑1 (MQ=255)
                                                                                                                 tCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                          <  1:60299/289‑1 (MQ=255)
                                                                                                                      cGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACtgtg                                                                                                                                                     <  1:430742/289‑1 (MQ=255)
                                                                                                                       gggCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATg                                                                                                                                                                                         >  1:352464/1‑252 (MQ=255)
                                                                                                                       gggCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATg                                                                                                                                                                                         <  2:352464/252‑1 (MQ=255)
                                                                                                                                       gggAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGgtcgtc                                                                                                                                     >  2:410021/1‑288 (MQ=255)
                                                                                                                                              tGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAagg                                                                                                                                                                                                   <  2:1291817/219‑1 (MQ=255)
                                                                                                                                              tGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAagg                                                                                                                                                                                                   >  1:1291817/1‑219 (MQ=255)
                                                                                                                                                                 gCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGcc                                                                                                          >  1:1982301/1‑289 (MQ=255)
                                                                                                                                                                   cACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAcc                                                                                                                                                                                                                                                  <  2:539989/151‑1 (MQ=255)
                                                                                                                                                                   cACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAcc                                                                                                                                                                                                                                                  >  1:539989/1‑151 (MQ=255)
                                                                                                                                                                   cACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTTCGCTTTGTACCGCAGACGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCCTGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTTGGGTACCAGccc                                                                                                         >  2:1877887/1‑287 (MQ=255)
                                                                                                                                                                    aCGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCTGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCa                                                                                                                                    >  2:1810173/1‑260 (MQ=255)
                                                                                                                                                                        cgtgcggAACCGGGCTGACGTGTTCCGGAAGGTT‑TGTGCGGGGTCGGCGGGACGAAGACTGGTGTTTAGCCGCTGGCAACGTCGGGTGGCTGGCAGTTGATTGGTCATTCCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGGCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGGGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcg                                                                                                    <  2:1758865/282‑1 (MQ=255)
                                                                                                                                                                        cgcgcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcg                                                                                                    <  1:1002811/288‑1 (MQ=255)
                                                                                                                                                                         gcgcGGGACCGGGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGGAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcgg                                                                                                   <  2:1172257/288‑1 (MQ=255)
                                                                                                                                                                                               tCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCgg                                                                                                                                                                      >  1:833998/1‑199 (MQ=255)
                                                                                                                                                                                               tCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCgg                                                                                                                                                                      <  2:833998/199‑1 (MQ=255)
                                                                                                                                                                                                ccGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACg                                                                           <  1:410021/289‑1 (MQ=255)
                                                                                                                                                                                                ccGGCAGGTTCCTG‑CGGGGTCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACg                                                                           <  2:638979/289‑1 (MQ=255)
                                                                                                                                                                                                       gTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  1:1763721/193‑1 (MQ=255)
                                                                                                                                                                                                       gTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGc                                                                     <  1:1593345/288‑1 (MQ=255)
                                                                                                                                                                                                         tCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                          <  2:777064/201‑1 (MQ=255)
                                                                                                                                                                                                         tCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                          >  1:777064/1‑201 (MQ=255)
                                                                                                                                                                                                          cTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAAc                                                                 <  1:1821170/289‑1 (MQ=255)
                                                                                                                                                                                                          cTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCAGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAAc                                                                 <  1:1577417/289‑1 (MQ=255)
                                                                                                                                                                                                            gT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCt                                                               >  1:215593/1‑289 (MQ=255)
                                                                                                                                                                                                                      gctcggCCGTCGACTGGACTTTATCCCGTGGCCACGCCGTGTGGCTGGCAGTTGATTGGTCATACCTCACCCAGCCTGTTTGGTCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGCGACAGCGTGCGCTTGGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACtggtgg                                                       <  2:1049475/284‑1 (MQ=255)
                                                                                                                                                                                                                        cggGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTCTTCGCCATTCGGGGTGCAGCCACTGCGGCCCCGTTGATATGCCCGCGTTACGCCTTGCTAACCTTCTGGGGGGTa                                                    >  2:1399750/1‑289 (MQ=255)
                                                                                                                                                                                                                         gggCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACGGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTa                                                    <  1:1399750/288‑1 (MQ=255)
                                                                                                                                                                                                                             cGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGAc                                               <  1:1727039/289‑1 (MQ=255)
                                                                                                                                                                                                                                    tGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATg                                        <  1:506574/289‑1 (MQ=255)
                                                                                                                                                                                                                                      gtgtTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                    <  2:1743834/163‑1 (MQ=255)
                                                                                                                                                                                                                                      gtgtTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGc                                       >  1:1564950/1‑288 (MQ=255)
                                                                                                                                                                                                                                            aTCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGGTATGACGCCACTGgcccccc                                 >  2:1243743/1‑286 (MQ=255)
                                                                                                                                                                                                                                              ccGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCCCGGTCCGCATTGCTAACCTACTGGGGGGTAATGACGCCAATGCCCCcgc                                 >  2:82100/1‑286 (MQ=255)
                                                                                                                                                                                                                                                 cTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCCCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGACCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGATTTCGCCAGTCGGGTATCCGCCACTGCAGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACTCCAATGCCCCCGCTCTggc                           >  1:1253406/1‑288 (MQ=255)
                                                                                                                                                                                                                                                 cTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTAATACCCCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCGACCGCGGCGCTCAGGATATGCCCGCGGTACCCAGTGCTAACGCACTGTGGGGTAATGACGCAAATGGCCCCACGCgggg                           >  1:581025/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           cGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTc                 <  2:1982301/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               tggctggcAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGt              <  1:1243743/288‑1 (MQ=255)
                                                                                                                                                                                                                                                               tggatggcAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGt              <  1:627515/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tggcAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCt                                                                    <  1:147311/230‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tggcAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCcg                                  <  1:979205/264‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tggcAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCcg                                  >  2:979205/1‑264 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                
GGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGT‑CGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCAGTCTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGTGCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGAGCGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTT  >  CP042583/606709‑607262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A