Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 606,988 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583606,9880AT98.4% 711.2 / NA 305intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (125/173);  minor base C (5/0);  total (131/174)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583606,9890GC100.0% 729.5 / NA 305intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (131/174);  total (131/174)
*CP042583606,9900TA99.0% 723.5 / NA 305intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (128/174);  minor base C (3/0);  total (131/174)
Fisher's exact test for biased strand distribution p-value = 7.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

GGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCAGTCTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGAGCGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTG  >  CP042583/606709‑607263
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                  
ggtggtGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGc                                                                                                                                                                                                                                                                              <  1:637323/288‑1 (MQ=255)
     tgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGttt                                                                                                                                                                                                                                                                        >  1:1935916/1‑289 (MQ=255)
              aCCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGgc                                                                                                                                                                                                                                                                >  1:1344826/1‑288 (MQ=255)
              aCCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGCCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTACGCCTGTTTGATCCGgcg                                                                                                                                                                                                                                                               >  1:1676734/1‑289 (MQ=255)
                    gttGGCGGTTGTCGCGGCGCATTGCGGCTTGAGCGAAAGACAGGTTGTTGAATTGCACCCCTCCTGGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGa                                                                                                                                                                                                                                                          <  1:1257696/287‑1 (MQ=255)
                         gggttgtCGCGTCGGATGGGGGGGTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAGCCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     <  2:220978/287‑1 (MQ=255)
                         cGGTGGTGGCGGCGCATTGCGGGTTGAGCGAAAACCAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACCCACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     <  1:2356725/288‑1 (MQ=255)
                             ggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGCCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTTATTGGTCAGACCGCACTCGGCCTGTTTGAGCCGGCGCGTgcggaaaccttt                                                                                                                                                                                                                                                >  2:817199/1‑278 (MQ=255)
                                     cgcATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTAc                                                                                                                                                                                                                                         <  2:768124/288‑1 (MQ=255)
                                                     cGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATGCCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGt                                                                                                                                                                                                                         <  1:2083317/288‑1 (MQ=255)
                                                        aaaaCAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑gcg                                                                                                                                                                                                                     <  1:1305366/288‑1 (MQ=255)
                                                                ttgttgAATTGCACTCCTCCGTGGAATACGTGGTGTGGGTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGt                                                                                                                                                                                                               <  1:1027077/286‑1 (MQ=255)
                                                                 tgttgaatgtagctccgcCGTGGAATACTGGGGCTGGTTTTGAGGTTTTCAACCGGGCTTCCCGGATCTCGGGAGTTTGCCGGAACAAC‑TCCACACGCCACGGCGCGCTGAACCGCGCTTGCTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCg                                                                                                                                                                                                           <  2:2642903/277‑1 (MQ=255)
                                                                 tggtgAATTGCCCTCCTCCGTGGATTACGTGGTCTGGTTTTTAGGTTTTCAACTGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGAATGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCg                                                                                                                                                                                                           <  2:2719298/286‑1 (MQ=255)
                                                                        ttGCACTCCACGGTGGACTCCGGGGTCTGGTTTTTAGGTTTTCACCCGGGCTTCCCGGATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAagg                                                                                                                                                                                                    <  2:675099/289‑1 (MQ=255)
                                                                                ctccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     >  1:2116617/1‑233 (MQ=255)
                                                                                  ccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCg                                                                                                                                                                                                           >  1:225336/1‑272 (MQ=255)
                                                                                    gTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCt                                                                                                                                                                                        <  1:70495/289‑1 (MQ=255)
                                                                                    gTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCt                                                                                                                                                                                        <  1:2389236/289‑1 (MQ=255)
                                                                                     tGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGTGGGAGTATGCTg                                                                                                                                                                                       >  1:1834412/1‑289 (MQ=255)
                                                                                      ggaagtcggggtttggtttTTAGGATTGCAACCGGGCTTCCCGGATCTCGGGAGTTTGCCGGAACAAC‑TACCAACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGAGCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGTCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTg                                                                                                                                                                                       <  2:1043628/279‑1 (MQ=255)
                                                                                        aaTACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTTACGAACCCATCTTCTTACGTCCGGGGGACAGCCTCCCCCTTTGCCCCG‑AGAAGGAGGGAGGATGCTGAAg                                                                                                                                                                                    >  2:2448648/1‑289 (MQ=255)
                                                                                            cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCg                                                                                                                                                                                                           >  1:2491246/1‑262 (MQ=255)
                                                                                                  gtGGGTTTTATGTCTTCGACCGGGCTGCCCGTATGTCGGGAGGTTGCCGGAACAAC‑TACACCCGCCACGGCGCGCTGGACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGAGCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTg                                                                                                                                                                          <  2:1834412/288‑1 (MQ=255)
                                                                                                  cTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTg                                                                                                                                                                          <  1:1624254/289‑1 (MQ=255)
                                                                                                      tttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCgg                                                                                                                                                                       <  2:499132/288‑1 (MQ=255)
                                                                                                       ttttGGGTTTTCACCCGGGTTTCCCGTATCTCGGGGGTTTGCCGGAACAAC‑TGCAACCGCCACGGCGGCCTGAACCGCGGTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:1299064/289‑1 (MQ=255)
                                                                                                       ttttCGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:2040588/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:928525/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:2016124/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:1928084/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:360001/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:2641873/289‑1 (MQ=255)
                                                                                                       ttttAGGTTTCCACCCGCGGTGCCCGTGTCGCGGGAGTTTGCCGGAACAAC‑TACGCACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:1544044/289‑1 (MQ=255)
                                                                                                       ttttAGGTTGTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:2400269/289‑1 (MQ=255)
                                                                                                       ttttAGGTCTTCAACCGGGCTTCCCGTATCACGGGGGTTTGCCGGAACAAC‑TACAGACGCCAGGGCGCGCTGAGCCGGGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:2511487/289‑1 (MQ=255)
                                                                                                       tgttAGGTTTTCAACCGGGCTTCCCGTATTTCGGGGGTTTGCCGGAACCAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:133925/287‑1 (MQ=255)
                                                                                                       gtttAGGTTTTCAACCGGGCTTCCCGTATGTCGGGAGTTTGCCGGAACAAC‑TGCACCAGCCGCGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCGGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:1704108/288‑1 (MQ=255)
                                                                                                              ttttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAaggagg                                                                                                                                                                                                 <  1:118002/254‑1 (MQ=255)
                                                                                                                ttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATAcc                                                                                                                                                            >  1:1453831/1‑289 (MQ=255)
                                                                                                                 tcacacgcgCTTCCGGGAGCTCGGGGGTTGACCGGAACAAG‑TGCACACGCCTCTGCGGGCTGAACCGCGTTTACTCGTACCGGCGGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGGTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                           <  2:2739974/281‑1 (MQ=255)
                                                                                                                 tCAACCGGGCTGCCCGTATCTCGGGGGTTTGGCGGAACAAC‑TACACACGCCACGGCGGGCTGGACCGCGCTTAGTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                           <  2:2344486/289‑1 (MQ=255)
                                                                                                                  cacgcGGGCTGCCCGACTCTAGGTAGTTTGCCGG‑ACAACGCACACACGCCCGGGGGCGCTGAACCGCGTGTACTCGGTCGGGCAGGTGCTGTCGGGATGGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCGGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCa                                                                                                                                                           <  2:2111803/284‑1 (MQ=255)
                                                                                                                                   tctcGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGc                                                                                                                                                                                                                                                                              <  2:942701/157‑1 (MQ=255)
                                                                                                                                   tctcGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTcc                                                                                                                                                                                                                                     <  1:815041/198‑1 (MQ=255)
                                                                                                                                       gggAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTAc                                                                                                                                                                                                                                         <  1:797480/190‑1 (MQ=255)
                                                                                                                                       gggAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTTTGCAGGATGGCGgtcgtc                                                                                                                                      >  1:1540454/1‑288 (MQ=255)
                                                                                                                                        ggAGTTTGCCGGAACAAC‑AACCCACGCCACGGCGCGCTGAACCGCCCTTACTCGTTCCGGCAGGTGCTGTCGGGATCGGCGGGCCGCATACTGGTGTTTATCCGCTGGCAACGCCGTGTGGCTGGCAGTTGATCGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCa                                                                                                                                     <  1:654210/288‑1 (MQ=255)
                                                                                                                                            tttGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAagg                                                                                                                                                                                                    >  1:425684/1‑221 (MQ=255)
                                                                                                                                              tGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGCCAGCGT‑GCGCTTTGTACCGCAGAAGGTGGGAGTAGTCTTAACATTATTCGTGCGGCCATGTCTACCACTGTGCAGGGTGGGGGTGGTAACGGttt                                                                                                                               >  2:197355/1‑288 (MQ=255)
                                                                                                                                              tGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     >  2:722063/1‑250 (MQ=255)
                                                                                                                                                      caac‑TCAACACGCTGCGGCCGGCTGGGCCGCGATTACTCGTTCCGTCAGGTTCTGTCGGGATAGGCGGGCCGCGGAGTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCagtc                                                                                                                      <  2:2381271/289‑3 (MQ=255)
                                                                                                                                                                ccggcACGGGGCGCTGAACGCGGCTTACTCGTTCCGGCAGGTTCTGGCGGGAGCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAg                                                                                                             <  2:2033906/285‑1 (MQ=255)
                                                                                                                                                                  gCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGgaga                                                                                                                                                                                                                               <  2:1453967/174‑1 (MQ=255)
                                                                                                                                                                  gCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGcc                                                                                                           >  1:475961/1‑289 (MQ=255)
                                                                                                                                                                       ggCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgc                                                                                                      >  1:730597/1‑289 (MQ=255)
                                                                                                                                                                       ggCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgc                                                                                                      >  1:1759924/1‑289 (MQ=255)
                                                                                                                                                                         cgcgcTGAACCGGGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcg                                                                                                     <  1:2543983/288‑1 (MQ=255)
                                                                                                                                                                         cgcgcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcg                                                                                                     <  2:461141/288‑1 (MQ=255)
                                                                                                                                                                          gcgcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  1:2622583/223‑1 (MQ=255)
                                                                                                                                                                            gcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     >  2:601748/1‑142 (MQ=255)
                                                                                                                                                                            gcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGgcgc                                                                                                 >  1:277662/1‑289 (MQ=255)
                                                                                                                                                                            gcTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGGATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcggcg                                                                                                  >  2:1359499/1‑288 (MQ=255)
                                                                                                                                                                             gtagatcgcgCTTGCTCGTTCCGGCTGGTTCTTTCGGGGTCGGCGGGCCGCGGACTGGTGGTTATCCGCTGGCAACGCCGGGTGGCTGGCAGCTGATTGTTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGTAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCa                                                                                                <  2:1196507/283‑1 (MQ=255)
                                                                                                                                                                              tGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCa                                                                                                <  1:1091119/288‑1 (MQ=255)
                                                                                                                                                                                     cgcTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGc                                                                                                            <  1:2724672/269‑1 (MQ=255)
                                                                                                                                                                                     cgcTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCa                                                                                                >  1:558128/1‑281 (MQ=255)
                                                                                                                                                                                        ttACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGc                                                                                      <  1:847367/288‑1 (MQ=255)
                                                                                                                                                                                               ttCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGtt                                                                               >  1:2349450/1‑288 (MQ=255)
                                                                                                                                                                                                tCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGCTATGCCCGCGTTAc                                                                             >  1:100225/1‑289 (MQ=255)
                                                                                                                                                                                                 ccGGCAGGTTCTGTCGGGATCGGCGGGGCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATTCCTCACTCAGCCTGTTTGATCCGGCGAGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCGTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACg                                                                            <  2:1617901/289‑1 (MQ=255)
                                                                                                                                                                                                 ccGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACg                                                                            <  2:2583825/289‑1 (MQ=255)
                                                                                                                                                                                                        gTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGTGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     >  2:432179/1‑193 (MQ=255)
                                                                                                                                                                                                        gTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGc                                                                      <  1:930672/288‑1 (MQ=255)
                                                                                                                                                                                                         ttCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCt                                                                     <  2:2626595/288‑1 (MQ=255)
                                                                                                                                                                                                          tCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCagtcgg                                                                                                                    <  2:391958/240‑5 (MQ=255)
                                                                                                                                                                                                               cGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTa                                                               <  1:1276421/288‑1 (MQ=255)
                                                                                                                                                                                                                   aTCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTAc                                                                                                                                                                                                                                         >  1:1722007/1‑115 (MQ=255)
                                                                                                                                                                                                                   aTCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTAc                                                                                                                                                                                                                                         <  2:1722007/115‑1 (MQ=255)
                                                                                                                                                                                                                        cggGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     <  1:1412749/98‑1 (MQ=255)
                                                                                                                                                                                                                        cggGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAAc                                                                                                                                                                                                                                                     >  2:1412749/1‑98 (MQ=255)
                                                                                                                                                                                                                        cggGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGGTATGCACGCGTTACGCTTTGCTAACCTACTGGGGGGTa                                                     >  2:348094/1‑289 (MQ=255)
                                                                                                                                                                                                                         gggCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGAGTGGTCATACCGCAGTCAGCCTGTTGGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTaa                                                    <  1:951234/289‑1 (MQ=255)
                                                                                                                                                                                                                            ccGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTTCGGGCATGTATACCACTGTGCAGGATGGCGGTCGGCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATAGGCCCGCGTTACGCATTGATAACCTACTGGTGGGTACTGa                                                 >  2:2281505/1‑289 (MQ=255)
                                                                                                                                                                                                                               cAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     <  2:1988725/170‑1 (MQ=255)
                                                                                                                                                                                                                               cAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGc                                                                                                                                                                     >  1:1988725/1‑170 (MQ=255)
                                                                                                                                                                                                                                            aTCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCg                                                                                                                                                                        <  1:174126/154‑1 (MQ=255)
                                                                                                                                                                                                                                            aTCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCcgcg                                 <  2:1006406/289‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCCGGTTTCAGCCACGGCGGCGCCCTGGATATGCCCGCGTTACGCAGTGATATCCTAGCTGCGGGCTATGCCGCCAACcccccccgccc                               >  2:789721/1‑284 (MQ=255)
                                                                                                                                                                                                                                                gCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTgg                             >  1:1904542/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 cTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcggcg                                                                                                  <  1:1517444/219‑1 (MQ=255)
                                                                                                                                                                                                                                                 cTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTgcggcg                                                                                                  >  2:1517444/1‑219 (MQ=255)
                                                                                                                                                                                                                                                 cTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACg                                                                            >  1:1494061/1‑241 (MQ=255)
                                                                                                                                                                                                                                                   ggCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCagtc                                                                                                                      >  2:176130/1‑195 (MQ=255)
                                                                                                                                                                                                                                                      aaCGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATc                        >  1:401719/1‑288 (MQ=255)
                                                                                                                                                                                                                                                        cGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACg                     <  2:114680/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTc                  <  2:169289/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gctggcAGTAGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTGCGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCa             <  1:2519298/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cAGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTaa        <  1:197355/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aGTTGATTGGTCATACCTCACTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGTCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTCATGACGCCAATTACCCCGCGCTGGCGATCACGCTGGGTCAGTTAAcg      >  2:2706856/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                  
GGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAAC‑TACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCAGTCTCAGCCTGTTTGATCCGGCGCGTGACGAACCCATCTTATTACGTCCGGGAGACAGCGT‑GCGCTTTGTACCGCAGAAGGAGGGAGTATGCTGAAGATTATTCGTGCGGGCATGTATACCACTGTGCAGGATGGCGGTCGTCACGGTTTTCGCCAGAGCGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTG  >  CP042583/606709‑607263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A