Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F148 I1 R1	14	45.7	1660340	90.3%	1499287	142.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,643	G→A	intergenic (‑33/+427)	cyoA ← / ← ampG	cytochrome o ubiquinol oxidase subunit II/muropeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,643	0	G	A	100.0%	150.7 / NA	49	intergenic (‑33/+427)	cyoA/ampG	cytochrome o ubiquinol oxidase subunit II/muropeptide transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/28); total (21/28)

ATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAG  >  NC_000913/451504‑451778

aTCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCAttt                                                                                                                                      <  1:476986/143‑1 (MQ=255)

   tGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTaaa                                                                                                                                   <  1:403998/143‑1 (MQ=255)

   cgTCCGTTGGGGTCTAGCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACTACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTaaa                                                                                                                                   <  2:657558/142‑1 (MQ=255)

        tttGGGATCTAACAGCGCAGAATTACAGCCACTGAGAAATACAGTGGCTGCAAAAAATGACAACCATCCCAAACTTTTCTTGTATATCCTGAGTCTCATTTAACGACCTCAATTCCACGGCACCTGGTGGCATTTAAAgtgtg                                                                                                                              >  1:369262/1‑143 (MQ=255)

           tggATCTAACATCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAATTTTTTTGTAGTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTgg                                                                                                                           <  1:310147/142‑1 (MQ=255)

             gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCCATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGc                                                                                                                         >  2:715784/1‑143 (MQ=255)

               tCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCAt                                                                                                                       >  1:528873/1‑143 (MQ=255)

                     aGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACg                                                                                                                 <  1:293721/143‑1 (MQ=255)

                     aGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACg                                                                                                                 <  1:293723/143‑1 (MQ=255)

                     aGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCAGTTTACg                                                                                                                 >  2:252320/1‑143 (MQ=255)

                      ccgcAGAATTACAGCCACTGAGCAATACAGTGCCCGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACgg                                                                                                                <  1:258807/142‑1 (MQ=255)

                          aGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAg                                                                                                            <  1:584563/143‑1 (MQ=255)

                             agtACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGtt                                                                                                         <  2:783085/141‑1 (MQ=255)

                              ttACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTa                                                                                                        <  1:273959/143‑1 (MQ=255)

                                     cACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACt                                                                                                 <  1:682149/143‑1 (MQ=255)

                                      aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGa                                                                                                              >  2:156130/1‑129 (MQ=255)

                                      aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGa                                                                                                              <  1:156130/129‑1 (MQ=255)

                                      aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTg                                                                                                <  1:794565/143‑1 (MQ=255)

                                        tGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTa                                                                                              >  2:753909/1‑143 (MQ=255)

                                                    tGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTaaa                                                                                  <  1:242746/143‑1 (MQ=255)

                                                    tGCCTGCAAATAACGACAACCATCCCAAACTTTTATTTTATTTCCTTATTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTaaa                                                                                  <  1:257806/143‑1 (MQ=255)

                                                        tGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTcc                                                                              >  1:727118/1‑143 (MQ=255)

                                                         gCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCt                                                                             <  2:574257/143‑1 (MQ=255)

                                                                   aCAACCCTCCGAAACTTTTATTGGTTTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGa                                                                   <  2:82655/143‑1 (MQ=255)

                                                                      aCCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGAATCTGGTGGCATTTAAAGTGTGTGGGCATTATACCGGAAGGTTACATTACTGTCAACATGATTAAATTCCTGTTCCTTGGAtct                                                                >  1:615137/1‑141 (MQ=255)

                                                                           cccAAACTTTTGTTGTATTTCCTGACTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTg                                                           <  1:698785/143‑1 (MQ=255)

                                                                             cAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTTTGAGCATTTTACGGGAAGGATACATTACTGTAAACATGATTAAATTACTATTACGTGAATTTGGCTggc                                                         >  2:21031/1‑141 (MQ=255)

                                                                                 ctttgcttGTGTTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGGGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGt                                                     <  2:185519/137‑1 (MQ=255)

                                                                                  ttttATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGATACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGcctgttc                                                    >  1:339672/1‑138 (MQ=255)

                                                                                   tttATTGTGTTTCCTGAGTCTCATTTAACGAGCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTca                                                   <  2:703832/143‑1 (MQ=255)

                                                                                         gTATTTCCTGGGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATg                                             <  2:355484/143‑1 (MQ=255)

                                                                                                 tGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTCTAAACATGCTTACATTTGAGCTGCGTGGATTTGGCTGCCAGGTCACATATGTTGCaaaa                                     >  2:802810/1‑143 (MQ=255)

                                                                                                  tggTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAAc                                    <  1:552001/141‑1 (MQ=255)

                                                                                                      ctcATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACatat                                <  1:767576/143‑1 (MQ=255)

                                                                                                       tcATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATc                               >  1:72420/1‑143 (MQ=255)

                                                                                                          tttAACGACCTCAATTCCACGGGATCTGGTGGCATTTATAGAGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCGCATACGTTGCAAAACATATCAgg                            >  2:453351/1‑143 (MQ=255)

                                                                                                                gACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGAtt                      <  1:795494/143‑1 (MQ=255)

                                                                                                                    tCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTTGCTGCCAGGTCACATATGTGGCAAAACATATCAGGTTGATTGTaa                  >  1:353258/1‑143 (MQ=255)

                                                                                                                      aaTTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCGTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATCTCAGGTTGCTTTTaaga                >  2:497894/1‑143 (MQ=255)

                                                                                                                        ttCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCCCCCATGCACCAAAACATATCAGGTTGATTTTaagaag              >  1:780129/1‑143 (MQ=255)

                                                                                                                         tCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGGTTTTAAGAAGc             >  1:749055/1‑143 (MQ=255)

                                                                                                                            ccGGGATTTTGTGGCATTTAAAGTCTGTGTGCATTTTACGTGAAGGTTACATTACTGTAAGCATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGt          <  2:471285/142‑1 (MQ=255)

                                                                                                                            aCGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTACTGTTACATGGATTTGGCTGCCAGGTCACATATATTGCAAAACATATCAGGTTGATTTTAAGCAGCTGt          >  1:453090/1‑143 (MQ=255)

                                                                                                                             cGGGATCGGGTTGCATTTAAAGTGTGTGGGCACTTTCCGTGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGATGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGtt         <  2:365195/143‑1 (MQ=255)

                                                                                                                              cgcaTCTGGTGCCAGTCAAAGCGTGTGGGCATTTTACCGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTg        <  1:499655/140‑1 (MQ=255)

                                                                                                                                gATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCa      >  1:355991/1‑143 (MQ=255)

                                                                                                                                 aTCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGACGCTGTTGCaa     >  2:115456/1‑143 (MQ=255)

                                                                                                                                   cTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCaaaa   <  1:385583/143‑1 (MQ=255)

                                                                                                                                    tggtggCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAg  >  2:480070/1‑143 (MQ=255)

ATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAG  >  NC_000913/451504‑451778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A