Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F184 I2 R1	13	42.0	1528396	89.7%	1370971	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,643	G→A	intergenic (‑33/+427)	cyoA ← / ← ampG	cytochrome o ubiquinol oxidase subunit II/muropeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,643	0	G	A	100.0%	163.2 / NA	52	intergenic (‑33/+427)	cyoA/ampG	cytochrome o ubiquinol oxidase subunit II/muropeptide transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/31); total (21/31)

CTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGG  >  NC_000913/451506‑451779

cTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTaa                                                                                                                                     >  2:88977/1‑143 (MQ=255)

   tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTACCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCACTTCCACGGGATCTGGTGGCATTTAAGgt                                                                                                                                  >  1:31468/1‑143 (MQ=255)

     cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAgtgt                                                                                                                                >  2:647107/1‑143 (MQ=255)

          ggATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTggg                                                                                                                           >  2:52076/1‑143 (MQ=255)

           gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGGCAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGc                                                                                                                          <  1:598949/143‑1 (MQ=255)

           gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCCAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGc                                                                                                                          >  2:478501/1‑143 (MQ=255)

             tCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATGTGGTGGCATTTAAAGTGTGTGGTCAt                                                                                                                        >  1:290960/1‑143 (MQ=255)

                aaCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTATTATCGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTCAAAGTGTGTGGGCAtttt                                                                                                                     >  2:202335/1‑143 (MQ=255)

                    gcgcAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACgg                                                                                                                 <  1:457544/143‑1 (MQ=255)

                       cAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGaa                                                                                                              <  2:649671/143‑1 (MQ=255)

                        aGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAg                                                                                                             >  1:157207/1‑143 (MQ=255)

                        aGAATTACAGACACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAg                                                                                                             <  1:597042/143‑1 (MQ=255)

                             tACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTAc                                                                                                        >  1:613484/1‑143 (MQ=255)

                                 gCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTa                                                                                                    <  1:647107/143‑1 (MQ=255)

                                  ccACTTAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTAc                                                                                                   <  2:307247/143‑1 (MQ=255)

                                      tGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTa                                                                                               >  1:344881/1‑143 (MQ=255)

                                        aGCAAGACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTaaa                                                                                             <  1:118071/143‑1 (MQ=255)

                                             tACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGa                                                                                        <  1:430403/143‑1 (MQ=255)

                                              aCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGAt                                                                                       >  1:503149/1‑143 (MQ=255)

                                                       gcgaatcatGACTACCATCCCAAACTTTTGTTGTATTTCCTGCGTCTGATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCt                                                                              <  2:6970/140‑1 (MQ=255)

                                                       gCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCt                                                                              <  1:327591/143‑1 (MQ=255)

                                                       gCAAAGAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCt                                                                              <  1:190842/143‑1 (MQ=255)

                                                              atGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACtt                                                                       <  1:715366/143‑1 (MQ=255)

                                                                gACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCGCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTGCGGGGAGGTCGCAGTACTGTAAACATGATTAAATTCCTGTTACTTgg                                                                     >  2:737639/1‑143 (MQ=255)

                                                                 aCAACCGTCCCAATCTTTTATTGTATTTCCTGAGTCTCATATAACGACCTCAAGTCCACGGGATCGGGTGGCATTTAAAGTGTGTGGTCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGa                                                                    <  2:285849/143‑1 (MQ=255)

                                                                 aCAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCGTGTTACTTGGa                                                                    >  1:83009/1‑143 (MQ=255)

                                                                  cAACCATCCCACCCTCTTATTGTATTTCCTGAGTCCCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGAt                                                                   <  1:86820/143‑1 (MQ=255)

                                                                   aaCCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGTTGGCATTTAAAGTGTGTGGGAATTTTACGGGAAGGTTACATCACTGTAAACATGATTAAATTCCTGTTACTTgagtt                                                                  >  1:547680/1‑139 (MQ=255)

                                                                     ccATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTg                                                                <  2:503149/143‑1 (MQ=255)

                                                                        tCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACGTTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCt                                                             >  2:545150/1‑143 (MQ=255)

                                                                          ccAGACCTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGc                                                           <  1:208891/143‑1 (MQ=255)

                                                                             aaCTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAg                                                        <  1:733876/143‑1 (MQ=255)

                                                                                ttttATTGTATTTCCTGAGTCTCATGTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTc                                                     <  2:15564/143‑1 (MQ=255)

                                                                                       gTATTTCCTGAGTCTCATTTAACGACATCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGAATCTTAAGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATg                                              >  2:228267/1‑143 (MQ=255)

                                                                                         agtgacggAGTCTAATTTAACGACCTCAATGTCACGGGATCGGGTGGCATTTAAAGTGTGTTGGCAGTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGtt                                            <  1:30197/136‑1 (MQ=255)

                                                                                         aTTGCCTGAGTCTCATTTAACGACTGCACTTCATGTCGTGTTTTTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGtt                                            <  2:737566/143‑1 (MQ=255)

                                                                                               tGAGTCTCATTTAGCGACCTCAATTCCACGGGATCTGGTGGCATGTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCaaaa                                      <  1:582385/143‑1 (MQ=255)

                                                                                               tGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCaaaa                                      <  1:266180/143‑1 (MQ=255)

                                                                                               tGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCaaaa                                      <  2:241292/143‑1 (MQ=255)

                                                                                                  gTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACat                                   <  1:279421/143‑1 (MQ=255)

                                                                                                        tttAACGACCTCAATTTCACGGGATCTGGTGGCATTTAAAGTGTTTGCGTATTTTTCGGGAAGGTTACATTACTGTAAACATGATTAAATTGCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAgg                             <  2:175305/143‑1 (MQ=255)

                                                                                                          tAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTg                                           <  1:128918/127‑1 (MQ=255)

                                                                                                          tAACGACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTg                                           >  2:128918/1‑127 (MQ=255)

                                                                                                              gACCTCAATTCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTGGAtt                       >  1:420942/1‑143 (MQ=255)

                                                                                                                ccTCAATTCCACGGGAGCTGGCGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACAGGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGAtttt                     <  1:142642/143‑1 (MQ=255)

                                                                                                                  ttaaTTCCACGGGCTGTGGTGGCATGTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATGCCTGCTACTTGGCTTTGGCTGCGAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTaa                   <  1:144774/141‑1 (MQ=255)

                                                                                                                       tCCACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGc              <  1:239329/143‑1 (MQ=255)

                                                                                                                         cACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTACATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTg            >  2:50799/1‑143 (MQ=255)

                                                                                                                         cACGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTg            >  1:262384/1‑143 (MQ=255)

                                                                                                                           cGGGATCTGGTGGCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGtt          <  1:484284/143‑1 (MQ=255)

                                                                                                                                tgtggtggTATTTAAAGTGTGTGGCCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATGTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCaaa     <  2:360586/141‑1 (MQ=255)

                                                                                                                                   ggtggCATTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAgg  >  1:442849/1‑143 (MQ=255)

CTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGG  >  NC_000913/451506‑451779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A