Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F184 I2 R1
|
15 |
56.1 |
2030262 |
91.3% |
1853629 |
141.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,164,899 |
G→A |
R359C (CGC→TGC) |
parC ← |
DNA topoisomerase IV, subunit A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,164,899 | 0 | G | A | 95.5%
| 53.7
/ NA
| 22 | R359C (CGC→TGC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/15); minor base T (0/1); total (6/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164759‑3165038
|
cgACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGCGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGACGGcaca > 2:695608/1‑143 (MQ=255)
gcgcCGGTTTCGGTTCATCCTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATcg < 1:837136/143‑1 (MQ=255)
ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGGGGATATGCAGGCGCTTGAGGGCTTTCTCCAGCCGATAGTTCAGTCGCGGACACTCGGTGTCGCGGCGGACCACCCGCCAtt > 2:759735/1‑143 (MQ=255)
ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGCCCTTCGCCAAACGATAGTTCAGTCGGCGGCACACGGTCTCGCGGCGGAGCCCCAGCCAtg > 2:116398/1‑142 (MQ=255)
aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa < 1:397894/119‑1 (MQ=255)
aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa > 2:397894/1‑119 (MQ=255)
tCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAgg < 1:565937/143‑1 (MQ=255)
agaAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCAGAGGAACACCAGCCAATCCGAGAGGGTTTACAGGAGGATGTTCACCGCCGgaca > 1:727036/1‑142 (MQ=255)
tcaGCAAACCTTCGTGGCTTTGCTGGCGCTTGTGGATTTTCTCTACTCTATATTTCAGTCGGCGTCACATGTTATCGCTGCTGAACACCAGCCATTCGGAGAGGATTTCCAGTAGGTTTTTCACCGCCGGACGACCATCCAGa < 2:563038/142‑1 (MQ=255)
aaaCCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGAt < 1:330825/143‑1 (MQ=255)
tggATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 2:325471/142‑1 (MQ=255)
cgtatatGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGTCTCACGTTATCGCAGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 2:818812/140‑1 (MQ=255)
aGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 1:880909/143‑1 (MQ=255)
ggATATGCAGGCGCTTGAGGAGTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAgg < 1:264669/143‑1 (MQ=255)
gcgcTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGAt < 1:889793/143‑1 (MQ=255)
gAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACGGTCCGGAGCGACGAGACAAAGGCTAATGCGCTAGctcg > 2:442999/1‑142 (MQ=255)
ggACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCttt < 1:116398/143‑1 (MQ=255)
gTTCAGTCGGCGGCACACGGTAGCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGa < 1:458544/143‑1 (MQ=255)
ttCCGTCGGCGTCACACGGTATCGCGTCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAATGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 2:1011610/143‑1 (MQ=255)
tccatccggcggCAGACGTTACCGCGGGGGACCAACAGCCATTCGCAGAGGATTTCCAGCAGGTGTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 2:642610/137‑1 (MQ=255)
gTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:917116/143‑1 (MQ=255)
tCGGCGGCATACGGGATCGCGGCGGAGCACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt < 2:742547/143‑1 (MQ=255)
tCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATGCGATGGCTCTTTGCCAGATCGGTGGCGGCGCAGCggt > 2:360772/1‑143 (MQ=255)
gcggcaCACGGGATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt < 1:314918/143‑1 (MQ=255)
ggcaCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtc < 1:846838/143‑1 (MQ=255)
|
CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164759‑3165038
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
