Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F113 I2 R1
|
19 |
51.3 |
1830242 |
91.7% |
1678331 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,415,466 |
Δ1 bp |
coding (720/2145 nt) |
pta → |
phosphate acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,415,466 | 0 | T | . | 97.8%
| 195.0
/ ‑1.6
| 47 | coding (720/2145 nt) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (20/25); minor base C (0/1); total (20/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGT > NC_000913/2415331‑2415603
|
ctctTCCGCAGCTACAGTACACAATGTTGATCCGGCGCAGCTGCAAGAGTCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACcat < 1:679521/143‑1 (MQ=255)
ctctTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCCCTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACcat < 1:466528/143‑1 (MQ=255)
tCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCaccatc > 1:911475/1‑143 (MQ=255)
aaaGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATGATCAAc > 1:464564/1‑143 (MQ=255)
aaaGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAAc > 1:762917/1‑143 (MQ=255)
aaaGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCACCCT‑TGATTTCTGCTGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAAc < 1:208778/143‑1 (MQ=255)
aGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGa > 1:640851/1‑143 (MQ=255)
aaCAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATc > 1:329472/1‑143 (MQ=255)
tgctCCGGCGAAGCTGCAAGCTTCCGGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGCCTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTcg < 2:465355/140‑1 (MQ=255)
tGATTCGGTGAAGCTGCAACAATCAAGCCGGCGGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTcg < 1:333673/143‑1 (MQ=255)
ggcgccgctgctGGACTCCAGCCGGCTGCCGGTCCG‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGt < 2:460346/137‑1 (MQ=255)
cctcAAGATTCCATCCCCCTGCCTGTACT‑CGGCTGTGTGCCGCGGAGCTTTGACCTGATCCCGACTCGTGCGATCGATATGGCACACCACCTGAA‑GCGACTATTATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATcc < 2:131193/140‑1 (MQ=255)
tGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCCACGAAGGCGCCATCAATACTCGCCGCGTTAAATCCg > 1:322608/1‑143 (MQ=255)
cAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGCA‑CCGACCATCATCAACGAAGGCGACATCACTACTCGCCGCGTTAAATCCGTc > 2:761142/1‑143 (MQ=255)
aagaagCCAGCCCGCTGCCGGTTCT‑CGGGGCTGTGCCGTGGAGCGTTGACCTGATCGCGACTCGTGCGATCGATAGGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAAGACGCGCCGCGTTAAATCCGTCa < 2:161184/143‑1 (MQ=255)
ccAGCCCGCCGCCGGTTCT‑CGGCGCTGGGCCGTGGACCTTTGACCTGATCGCGACGCGTGCGAGCGATATTGCGCGCCACCTGAA‑GCGACCATCATGAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCt < 1:415078/143‑1 (MQ=255)
aGCCCGCGGCCGGTTCG‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTgc < 1:154259/143‑1 (MQ=255)
cccGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGACGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTgcgc > 1:718651/1‑143 (MQ=255)
cccGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTGTCTgggc > 1:525561/1‑143 (MQ=255)
cccGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCACCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTgcgc < 1:879508/143‑1 (MQ=255)
gcccGGGTCT‑CGGCGCTGTGGCGTGGGGCTTTGACGTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGcgcacgca < 1:516170/141‑1 (MQ=255)
t‑cGGCGCTGCGCCGTGGAGCTTGGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGc < 1:511339/143‑1 (MQ=255)
ggCGCCGCGCCGTTGAGCTTTGACCTGATCGCGCCTCGTGCGATCGAGATGGCTTGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGcac < 2:318813/143‑1 (MQ=255)
cTGTGGCGTGGAGCTTTGACCTGAGCGCGACTCGTGCGAGCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGc < 1:397471/143‑1 (MQ=255)
tgCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTgg < 1:692566/143‑1 (MQ=255)
gCCGCGGCGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGCA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGa < 2:718597/143‑1 (MQ=255)
tttaCCTGCTTGCGGCTCGTGCGATCGATATGGCTCGCCACCTG‑AGGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAAGCCGTCACTTTCTGCGCACGCAGCAGTCCGCACATGCTGGAGCACTTCCGTg < 2:20190/140‑1 (MQ=255)
tGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGtt > 1:809166/1‑143 (MQ=255)
gATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGTGACATCAATACTCGCCGCGGTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCCCATGCTGGAGCACTCCCGTCCCGGCtc > 2:793336/1‑143 (MQ=255)
aTCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtct < 1:36546/143‑1 (MQ=255)
tCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTtctc > 1:561891/1‑143 (MQ=255)
cgACTCGAGCGAACGATATGGCGCGCCACCTGTA‑GCGACCATCATCAACGAGGTCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCCCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgc < 1:146901/143‑1 (MQ=255)
caCTCGTGCGATCGATATGGCTCCCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTctgct < 1:484730/142‑1 (MQ=255)
tCGTGCGATCGATATGGCTCGCCACCTG‑ACGCGACCATCATCAAGGAAGGCGACATCAATACGCGCCGCGTTAAAGCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGt < 2:342824/143‑1 (MQ=255)
gTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGa < 2:206044/143‑1 (MQ=255)
gTGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGGTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGACCTTCCGTGCCGCTTACCTGCTGGTGa > 2:27938/1‑143 (MQ=255)
tGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCCATACTCGCCGCGGTAAATACGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGATCTCTGCTGGGGAc > 2:260064/1‑143 (MQ=255)
tGCGATCGATATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGAc < 1:369109/143‑1 (MQ=255)
cgatcgatATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTAGCCGCGGTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTGTCTGCTGGTGGCtt > 1:344647/1‑143 (MQ=255)
gatcgatATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTc > 2:610657/1‑143 (MQ=255)
gatcgatATGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTc < 2:783295/143‑1 (MQ=255)
tatGGCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGCCTTCCGCAGa > 1:201222/1‑143 (MQ=255)
gCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCGGCAGACCga > 1:20521/1‑142 (MQ=255)
gCTCGCCACCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCCGGTGACTTCCGCAGACCGt > 2:121496/1‑143 (MQ=255)
cTCGCCGCCTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCGGCGATAGTTCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTc < 2:318945/143‑1 (MQ=255)
tCGCCACCTGAA‑GCGACCATCATCAAGGAAGGCGACACCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCGGGAGCACTTCCGTGCCGCTGCTCTGCTGGTGACTGCCGCGCACCGTcc > 2:748944/1‑143 (MQ=255)
ccTGAA‑GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGGCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGt < 1:763171/143‑1 (MQ=255)
|
CTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCT‑CGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAATGCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGT > NC_000913/2415331‑2415603
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
