Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F9 I0 R1	81	94.4	2603848	74.4%	1937262	227.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,604	T→A	14.6%	I55F (ATC→TTC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,604	0	T	A	14.6%	189.6 / 2.4	87	I55F (ATC→TTC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand): ref base T (54/17); new base A (6/9); total (61/26)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065368‑4065833

catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑a                                                                                                                                                                                                                                      >  1:979634/1‑238 (MQ=255)

catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑a                                                                                                                                                                                                                                      >  1:978606/1‑238 (MQ=255)

catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTAAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑a                                                                                                                                                                                                                                      >  1:616001/1‑238 (MQ=255)

   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATc                                                                                                                                                                                                                                   >  1:518374/1‑238 (MQ=255)

   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGGTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCCTATAAAATAAAGGGACGAAATTTGCCTTTAGGCACAATATTTT‑T‑ACGCGAGTCGAGTAAAAAACAGGTG‑AGCATACCGGTAAACG‑CGGTAAAAAAATGCGACACCAGAAAGaa‑aacc                                                                                                                                                                                                                                   >  2:361018/1‑236 (MQ=255)

        tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCa                                                                                                                                                                                                                             >  1:148813/1‑239 (MQ=255)

        tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAAATTCGCCACCAGCAAGAT‑AATCCCGCCa                                                                                                                                                                                                                             >  1:710119/1‑239 (MQ=255)

        tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAACATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGGG‑AGCATATCGGTAAACG‑CGGGAAAAAACTTGTCCACACGAAAGAA‑AATCCCGCCa                                                                                                                                                                                                                             >  2:225152/1‑239 (MQ=255)

        tGTATGAAAGTCCAAACATCATAAACAGCGCCGTGGAAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACAAAAATTGCCTTTAGGCCCAATATTTT‑T‑TACCAAGTCGAGTAAAAATCAGGCG‑AGCATACCGGTAAAAT‑CGGCAAAAAACTTCGCCACCAGAAAGAA‑AATCCCGCCa                                                                                                                                                                                                                             >  2:626595/1‑239 (MQ=255)

          tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCAACCAGAAAGAT‑AATCCCGCCATa                                                                                                                                                                                                                           >  1:1018001/1‑239 (MQ=255)

           aTGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGGGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCGGAAAGAT‑AAACCCGCCATAg                                                                                                                                                                                                                          >  1:941898/1‑239 (MQ=255)

                aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGCTAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAAATTCGCAACCAGAAAGAT‑AATCCCGCCTTAGTagcc                                                                                                                                                                                                                     >  1:112242/1‑236 (MQ=255)

                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCa                                                                                                                                                                                                                    >  1:452165/1‑238 (MQ=255)

                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAg                                                                                                                                                                                                                   >  1:55382/1‑239 (MQ=255)

                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATACGGTG‑AGCATACCGTTAAACC‑CGGTAAAAAACTTCACCACCAAAAAGAA‑AAACCCCCCaaaaaaaaaag                                                                                                                                                                                                                   >  2:837019/1‑229 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGATTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAAAG‑CGGGAAAAAACTTCGCAACCAGAAAGAA‑AATCCCGCCAAAGaaaaaaagg                                                                                                                                                                                                                 >  2:846173/1‑233 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAGATCCGGTG‑AGCATATCGGAAAACG‑CGGTAAAAAAATTCGCCACCAGAAAGAA‑AATCCCGCCATAGGAAACAGGc                                                                                                                                                                                                                 >  1:598395/1‑239 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                 >  1:922083/1‑239 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGAAAACG‑CGGAAAAAAACTTTGCCACCAGAAGGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                 >  1:721744/1‑239 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTA‑AGCATATCGGTAAACG‑CGGTAAAAACCTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                 >  1:9148/1‑239 (MQ=255)

                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGGG‑AGAATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCCGCAAGAT‑AATCCCGCCATCGAAAGCAgg                                                                                                                                                                                                                  >  1:319770/1‑238 (MQ=255)

                            atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCTCTGCATATAAAATAAAGGGACGAAATTTGACTTTAGGCCCCATATTTT‑T‑CCGAGAGACGAGTAAAAATCCGTTG‑TGCCAATAGGTAACCC‑TGGTAAAAACCTTCGCAACCAGACAAAA‑AACCCCCCCAAATAAAACAGGCATACCaaa                                                                                                                                                                                                         >  2:1204148/1‑239 (MQ=255)

                            atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑GGGTAAAAAAATTCGCCACACGAAGGAT‑AATCCCGCCATAGTAACCAGGCATCCCt                                                                                                                                                                                                           >  1:408583/1‑237 (MQ=255)

                            atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTaa                                                                                                                                                                                                         >  1:667635/1‑239 (MQ=255)

                              aaaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGAAAAAAACTTTGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCAAAtc                                                                                                                                                                                                       >  1:291777/1‑238 (MQ=255)

                                   gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCCGAAAGAT‑AATCCCGCCATATTAAGCAGGCATCACTAATTCATCg                                                                                                                                                                                                  >  1:865917/1‑239 (MQ=255)

                                   gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGAAACCAGGCATCCCTAATTcctg                                                                                                                                                                                                   >  1:311397/1‑235 (MQ=255)

                                    cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                                 >  1:811831/1‑239 (MQ=255)

                                    cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                                 >  1:261733/1‑239 (MQ=255)

                                     gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAAtt                                                                                                                                                                                                       >  1:904744/1‑232 (MQ=255)

                                       ggTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat                                                                                                                                                                                              >  1:585503/1‑239 (MQ=255)

                                                 gtcgtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCTCGAGTAGGGTAAAAATCAGGTG‑CGCATATCGGTAAACG‑GGGTAAAAACCTTCGCAACCAGAAAGAG‑AATCCCGCCATATGAAGCAGCAAACCTAAATTAATAGGTAAAAAATTTgag                                                                                                                                                                                    >  2:261854/1‑239 (MQ=255)

                                                   cgtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga                                                                                                                                                                                  >  1:277425/1‑239 (MQ=255)

                                                                      aaGGTGGGGGAGAAAAACGGGAGTGTTGCAATTAACCCCCCCGGAA‑CCGCTGAATATAAAATAAAGGGACAAAATTTGCCTTTAGCCCCAATATTTT‑C‑CCGCGAGTCGAGTAAAAACCCGGTG‑AGCATATCGGAAAACG‑CGTTAAAAACCTTCGCCACCAGAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCa                                                                                                                                                               <  1:1086048/239‑1 (MQ=255)

                                                                       tggtggtGGATATAAAGTGGGGTTTTTCATTTCACCCCGCCGGAC‑CCTGTGAAAAAAAGAAAAAGGGACAAAATTTGCTTTTGGCCCAAATATTTT‑T‑CCGCTGGTGGAGAAAAAACCGGGTG‑AGCATATCGATAAACG‑CGTAAAAAACCCCCGCCACAAAAAAAAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag                                                                                                                                                              <  2:667635/238‑1 (MQ=255)

                                                                         gtggtgGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAATG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCGGCAAAAAAGTTGAGGAGAAACAAAGTACCGATGCaaag                                                                                                                                                            >  2:888658/1‑239 (MQ=255)

                                                                                    aaaCTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGATTCGAGTAAAAATCCGGTG‑AGCTTATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGTAGATACAGAGAACCGATACacagactacaattca                                                                                                                                                 >  2:760861/1‑228 (MQ=255)

                                                                                          gcggttgacAATTACCGCCGGCGGAC‑CCGTTG‑AAAAAAAATAAAAGGGCCAAATTTGGCCTTAGGGCCAATTTTTTCT‑CGGCGAGCTGTGAAAAAACCCGGTG‑ACAATTGCGGTAAACG‑CGGAAAAAACCTGCACCACAGGAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCg                                                                                                                                            <  2:922083/230‑1 (MQ=255)

                                                                                               ttGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTc                                                                                                                                       >  1:521077/1‑238 (MQ=255)

                                                                                               ttGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTAATCGGTATAAAATTTGAGGAGTTACAAAGAACCGCTGCAGAGCATTAAATTCGACCCGAcacc                                                                                                                                       >  1:289208/1‑235 (MQ=255)

                                                                                               ttGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCACAAAGAT‑AATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCGGGATACAATTTGAGGAGATAAAGAGTAACGATACAGAGCATTAAATTCGAAcagcaggc                                                                                                                                       >  2:555281/1‑231 (MQ=255)

                                                                                                tcctttttaccccgccgGAC‑CCTCTGAAATAAAAAAAAAAGGAAAAAATCTGCCTTGAGGCACAATTTGTT‑T‑CCGCGAGTAGAGAAAAAACCCGGAG‑GGCATACCGGTAAACG‑AGGTAAAAACCCGCCCCACAAAAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGc                                                                                                                                     <  2:710119/228‑1 (MQ=255)

                                                                                                  caaatttacccgcccGAACCCGCTGAAAAAAAAAAAAAGTGACGACATGTGCCTTTAGGCCAAATTATTG‑C‑CCGCGAGTACAATAAAAAGCCGGTG‑AGCTTACCGGTAAACG‑CGGTAAAAAACTTCGCCACAAGAAAAAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGcc                                                                                                                                    <  2:55382/230‑1 (MQ=255)

                                                                                                    aTTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCcca                                                                                                                                  >  1:834494/1‑238 (MQ=255)

                                                                                                       aaCGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGGG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCCAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGACTTAAATTCGAACCGACGGCGCccataa                                                                                                                               >  1:650847/1‑237 (MQ=255)

                                                                                                                             tataAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTc                                                                                                               >  1:411483/1‑233 (MQ=255)

                                                                                                                                aaaaaaagGGGAAAAATTTTGCTTTTAGGCCAAATATTTT‑C‑CCGCGAGTGGAGTAAAACTCCGGTG‑AGCATATGGGAAAACG‑CGTAAAAAACCTTCGCCCCAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  1:837019/239‑1 (MQ=255)

                                                                                                                                       aGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                                               >  1:470634/1‑239 (MQ=255)

                                                                                                                                       aGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGCACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                                               >  1:131707/1‑239 (MQ=255)

                                                                                                                                       aGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGTAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGACGATTCAGgttt                                                                                               >  1:722927/1‑239 (MQ=255)

                                                                                                                                         ggccGAAATTTCCCTTGAGGCCCAATCTTTT‑C‑CCGGGAATCAAATAAAAACCGGGTG‑AGCATACGGGAAACCG‑CGGTAAAAACCTTCACCACCAAAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                                               <  1:831254/234‑1 (MQ=255)

                                                                                                                                            cGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTCTAAAATTTGAGGAGATACAGAGTACCGATCCAGAGCATTAAATTCGCACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGCTTCAGTTTTAATGt                                                                                          >  1:513184/1‑239 (MQ=255)

                                                                                                                                            cGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGt                                                                                          >  1:363511/1‑239 (MQ=255)

                                                                                                                                            cGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACAGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCCGATTCAGTTTTAATGt                                                                                          >  1:1262948/1‑239 (MQ=255)

                                                                                                                                            aaaaaaTTGTCTTTGGGGCCAATTTTTT‑TCCCGGGAGGCGAAAAAAACCCCGGGG‑AGCATAGCGATAAACG‑CGGAAAAAAACTTCGCCACAAAAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                                            <  1:853689/236‑1 (MQ=255)

                                                                                                                                                    gCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAACAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATCAAATTTATGGAGATAACAAGTACCGATGCAGAGCATTAAATTGGAGCAGAGGTAGCCCAAACCACAGAGGATTTTTTTCCGCGGAATCATTTTTTATGTTAGTggca                                                                                  >  2:43970/1‑237 (MQ=255)

                                                                                                                                                    gCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAACGTCACGGGAt                                                                                  >  1:524240/1‑239 (MQ=255)

                                                                                                                                                         tAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCAACGCCGCCCATACCTAAGGCGGTTTTTTCCCCCAAATTAAATTTTAATGTCAGTGGATTAAgg                                                                             >  2:519614/1‑239 (MQ=255)

                                                                                                                                                          gtgcctattttttt‑t‑CCGAGCGTCGATAAAACACCCGGTG‑GCAATAT‑GGAAAAAG‑CAGTAAAAAAATTTCCCCCAAAAGAAATAAATCCCGCCATAGTAAGCAGTCATCCCTAATTCATCGGTATAAAATGTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  2:979634/229‑1 (MQ=255)

                                                                                                                                                          gggCCCAATTTTTT‑C‑CCGGGAGGCGGAAAAAAACCCGGTG‑AGCATAGGGGTAAACG‑GGGAAAAAATCTGCCCCACAAAAAAGAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  1:846364/238‑1 (MQ=255)

                                                                                                                                                          agcccaaaTTTTTT‑C‑CCGCGATTCGAAAAAAACCCGGGTA‑AGAAAATCGGTAAACG‑CGGTAAAAATCTCCGCCACCAGAAAAAT‑ACTCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  1:626595/233‑1 (MQ=255)

                                                                                                                                                           ggCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGCCAGTGGATTATGGTc                                                                           >  1:531357/1‑239 (MQ=255)

                                                                                                                                                           gaccaaaTTTTTT‑T‑CCGCGGGGCGAGTAAAAATCGGGTA‑AGCATAGGGATAAGCG‑GGGAAAAAAATGGCAACACAAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  1:589438/233‑1 (MQ=255)

                                                                                                                                                              cacatttttt‑c‑CCGGGAGTAGAATAAAAACCCGGGG‑AGCATCGCGG‑AAACGTAGATAAAAAACTTCCCAACCAAAAAAAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGa                                                                        <  1:1248463/233‑1 (MQ=255)

                                                                                                                                                                 atatTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCGGAATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTAGCCCATACAAAAGGCGATTTTTTCCCCCAAATTAAATTTTAATGTCAGTGGATTAAGGtaaaaaaa                                                                     >  2:622078/1‑232 (MQ=255)

                                                                                                                                                                      gt‑t‑CCGCGAGACGGATAAAACTCCGGTG‑AGAATTTCGGAAAACG‑GGGAAAAAAACTCCCCCCCCAAAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:1019316/238‑1 (MQ=255)

                                                                                                                                                                       t‑t‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGCCGTTGCCCATACCATAGGGGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGAGGATTTGAGTCAGACATAATCa                                                                >  1:581406/1‑238 (MQ=255)

                                                                                                                                                                        ttcccGGGAGGCGATTAAAAACCCGGTG‑AAAATTGCGGAAAACG‑CGTAAAAAACCTTCGCCACCAAAAAGAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:888658/236‑1 (MQ=255)

                                                                                                                                                                              cgagtcgagtAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCtt                                                          >  1:808970/1‑239 (MQ=255)

                                                                                                                                                                              cgagtcgagtAAAAATCCGGAG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGAAGAGCATTAAATTCGAACCGACGTCACCCATACCATAGGCGATTTTTTCCACCCGGTTCCGTTTTACATTAAGGGGATTAATGTCAGACATCATCACTCCtt                                                          >  2:783654/1‑239 (MQ=255)

                                                                                                                                                                                     agtAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACg                                                    >  1:817149/1‑238 (MQ=255)

                                                                                                                                                                                     aatAAAAACCCGGTG‑AGTATAGCGGAAAACG‑GGAAAAAAACCTTCCCCACAAAAAATAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACg                                                    <  1:760861/236‑1 (MQ=255)

                                                                                                                                                                                      attaaaaTCCGGGGTAGAATGTCGGAAACCG‑CGGAAAAAACCTTCCCCACAAAAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACg                                                    <  1:846173/235‑1 (MQ=255)

                                                                                                                                                                                                      gCATAGCGGTAAGCG‑CGTAAAAAACCTTCCCCAACAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcgtcgt                                    <  1:951204/238‑1 (MQ=255)

                                                                                                                                                                                                       cATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGGGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGttt                                  >  1:717117/1‑239 (MQ=255)

                                                                                                                                                                                                         tctgggaaaaCG‑GGGTAAAAACCTTCCCCACCAGAAAAAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  1:198133/232‑1 (MQ=255)

                                                                                                                                                                                                         tatCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATACTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCACGGGATTATGGTACGACATAATCCCTCCTTATAACGCCCCGCAGGGCGTCGttttt                                >  2:109180/1‑237 (MQ=255)

                                                                                                                                                                                                               tAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttattattat                          >  1:111583/1‑239 (MQ=255)

                                                                                                                                                                                                                 aaCG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAGACTCAGTTTTAATTTCAGTGGCTTTGGGTCAGACATAACCCCTCCTTCTAACCCCCCCCAGGAGCTCGTttttttttatt                         >  2:705927/1‑238 (MQ=255)

                                                                                                                                                                                                                    cgggtaaaaaaaCTTCCCCACCAAAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACg                      <  1:225152/233‑1 (MQ=255)

                                                                                                                                                                                                                         tAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGGTTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCCCAGGGCGTCGTTTATTTTTATTAcccccgg                 >  2:913996/1‑236 (MQ=255)

                                                                                                                                                                                                                               ccTTCGCAACAAAAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTa           <  1:622078/238‑1 (MQ=255)

                                                                                                                                                                                                                               catccgcagctaGAAAAAT‑ACCCCCGCCGTAGGAAGGATACATCCCTAATGCATAGGTATAAAATTTGAGGAGATCCAGAGTACCGATGCAGCGAATTAAATGCGAACAGACGTCGCCCATACCATAGGCGACTTTTTCCCGCAGATTCAGTTTTAATGGCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGTAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTa           <  2:112242/228‑1 (MQ=255)

                                                                                                                                                                                                                                 tccccccccaAAAATAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg         <  1:261854/232‑1 (MQ=255)

                                                                                                                                                                                                                                 ctCGCCACAAAAAAAAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg         <  1:361018/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cgccccaaaaaaaAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg         <  1:783654/228‑1 (MQ=255)

                                                                                                                                                                                                                                   cGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACgg        >  1:979239/1‑238 (MQ=255)

                                                                                                                                                                                                                                        caaGAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCt  <  1:1204148/237‑1 (MQ=255)

CATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAA‑CCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTT‑T‑CCGCGAGTCGAGTAAAAATCCGGTG‑AGCATATCGGTAAACG‑CGGTAAAAAACTTCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065368‑4065833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A