Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 43 55.9 1320120 86.3% 1139263 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,065,604 T→A 27.8% I55F (ATC→TTC)  yihO ← putative sulphoquinovose importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,065,6040TA27.8% 65.5 / 4.9 41I55F (ATC→TTC) yihOputative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (24/5);  new base A (4/8);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 3.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACG  >  NC_000913/4065378‑4065826
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAGTCCGG‑TGAG‑CATATCGGAAAAC‑GCGGAAAAAAACTTCGCCACCAGAAAAATa                                                                                                                                                                                                                               >  1:309267/1‑228 (MQ=255)
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATa                                                                                                                                                                                                                               >  1:517299/1‑228 (MQ=255)
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑GGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATa                                                                                                                                                                                                                               >  1:517502/1‑228 (MQ=255)
      aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAACGGTGGT‑GGCGCTAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAACTAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCAAGCAAAAATCCGG‑TGAG‑CAGATCGGAAAAC‑GCGGTAAAAAACTTGGCAACCAGAAAGATAATACCg                                                                                                                                                                                                                         >  1:583109/1‑228 (MQ=255)
      aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAACGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCCTATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGTGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GTGGTAAAAACCTTCCACCACAGAAAGAAAAAccct                                                                                                                                                                                                                         >  2:140193/1‑227 (MQ=255)
        tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGcc                                                                                                                                                                                                                       >  1:13971/1‑228 (MQ=255)
          cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGGAAAAAACTTCGCCACCAGAAAGAAAATccctgtc                                                                                                                                                                                                                      >  1:146533/1‑223 (MQ=255)
          cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGAAGAAATTTGCCTTTAGGCACAATATTTTTCCGAGATACGGGGAAAAATCCGGCTGA‑‑CATATCGGAAAAC‑GCGGTAAAAAACTTCACAAACAAAAAGAAAATCCCGccca                                                                                                                                                                                                                     >  2:347092/1‑226 (MQ=255)
                 catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACCGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGAAAGc                                                                                                                                                                                                              >  1:67994/1‑228 (MQ=255)
                          cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCACGAGTCGAGTAAAAATCCGG‑TGGG‑CATATCGGTAAAC‑CCGGGAAAAACCTTCGCCAACAGAAAGAAAATCCCGCCAAAGAAAGAAGGCAAccc                                                                                                                                                                                                     >  2:362839/1‑228 (MQ=255)
                          cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAAT‑AAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATACCt                                                                                                                                                                                                    >  1:342996/1‑228 (MQ=255)
                                                aCCGGCAAACAAAAGGTGGG‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                               >  1:486698/1‑228 (MQ=255)
                                                   cagaaaaaaaaGGGGGTGGGAAATAAAGGGGAGCG‑TGCAAATAACCGCCGCGGGCACCGTTCCATAAAAAAAAAAGGAAAGAATTTCCCCTTTGGCCACAATTTTTCCCCGAGGTTCGAGTAAAAACCGGG‑AGAT‑CTTACGGGAAAAC‑GCGGAAAAAAATTCCCCCACCAGAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                             <  2:202392/225‑1 (MQ=255)
                                                                   t‑ggCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGCAATTTACCTTTAGGCCCAATATTTTTCCGCGAGTCGCGTAACAATCCGG‑TGCG‑CATATCGGCAAAG‑CCGGTAAAAACCTTCACCACCAAAAAGATAACCCCGCCACAGTAACCAGGCATCCCAACTTCCTCGCAATAAAATTTAGGGAGATCCA‑AGTAcacga                                                                                                                                                           >  2:379137/1‑224 (MQ=255)
                                                                     ggCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGGTACAAGGTACCCAt                                                                                                                                                           >  2:530861/1‑228 (MQ=255)
                                                                                       tGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGCATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCg                                                                                                                                          >  1:433306/1‑227 (MQ=255)
                                                                                       tGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGAAGGCATCCCTAATTCCTCGGTATAAAATTTGAGGAGATACACGGTACCGATGCAGCGAATTAAATTCg                                                                                                                                          >  2:502923/1‑227 (MQ=255)
                                                                                                                              aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTCAGCAGGCATCCCTAATTCATCGGTATAAAAATTGAGGAGATACACAGTACCGTTGAAGCCCATTAAATTCGAACCGACGTCTCCCAAACCATAGGGGATTTTTTccccag                                                                                                   >  2:239209/1‑225 (MQ=255)
                                                                                                                              aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGACCCAACGTCGCCCATCCCATAGGCGATTTTTTCCCGCa                                                                                                   >  1:632113/1‑228 (MQ=255)
                                                                                                                              aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCcaca                                                                                                   >  2:444893/1‑228 (MQ=255)
                                                                                                                              aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCACCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCa                                                                                                   >  1:176460/1‑228 (MQ=255)
                                                                                                                                  agaaaaTTCGCTTTGGGGCCAAATATTTCTCCGAGAGTAGAGAAAAAATCGGG‑TGAG‑CATATCGGAAAAC‑GCGGTAAAAAACTCCACCACCAAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                               <  1:433280/225‑1 (MQ=255)
                                                                                                                                   cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTc                                                                                              >  1:463798/1‑228 (MQ=255)
                                                                                                                                                tAGGCCTAATTTTTTGCCGGGGATCGAATAAAAAGAGGG‑TGAG‑CATGGCGGAAACC‑GGGGAAAAAAACCGCGCACCCAAAAAAATAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 <  2:286555/228‑1 (MQ=255)
                                                                                                                                                tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga                                                                                                                                                                              >  2:182510/1‑135 (MQ=255)
                                                                                                                                                tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGAGGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGACa                                                                                 >  1:286555/1‑228 (MQ=255)
                                                                                                                                                tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCAAAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 >  1:502747/1‑228 (MQ=255)
                                                                                                                                                tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGCATAAAATTTGAGGAGATACAGGGTACCGATGCAGAGCATTAAATTTGAACCGACGTCGCCCATACCAAAGGCGAATTTTTCCTCCAAATCCAGTTTTaacttc                                                                                  >  2:239974/1‑223 (MQ=255)
                                                                                                                                                aaggcaaaaTGTTTTTCCGCGAGCTGA‑TAAAAAACCCG‑GGAGTCATCGCGGTAACC‑GGGGTAAAAATCTTCGCCACAAGAAGGAAAAACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 <  1:239209/221‑1 (MQ=255)
                                                                                                                                                 aGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                             <  1:182510/135‑1 (MQ=255)
                                                                                                                                                  gcccaaaTTTTTTCCCGCAAACCGAAAAAAACCCCGG‑GGAG‑CATACCGTAAAGC‑GCGATAAAAAACCTCCCCACGAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGt                                                                               <  1:475893/223‑1 (MQ=255)
                                                                                                                                                            ttttCCGGAGGTCGAATAAAAATCGGG‑TGAG‑ATTACCGGAAAAC‑GGGGAAAAAAACTCCGCCCCAAAAAAGAAAACCCCGCCATAGTTAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                     <  1:615454/228‑1 (MQ=255)
                                                                                                                                                                        tgaatAAAACTCCGG‑TG‑G‑CCTTTCGGGAAACGGAGGAAAAAAACTCCGCCAACAGAAAAATATGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                         <  2:159657/224‑1 (MQ=255)
                                                                                                                                                                                                     tAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCCGATTCAGTTTTAATGTCAGTGGATTTTGGTCACACATAATCACTCCTATTAACCCCCCTCAGGGggtgt                              >  2:150335/1‑226 (MQ=255)
                                                                                                                                                                                                     tAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                     >  1:506046/1‑189 (MQ=255)
                                                                                                                                                                                                       aaC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCGGAGATACAGAGTACCGATGCAGAGCATTAACTTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGCGGATTATTGTCAGACATAATCACTCATTATAACGCCCCGCAGGGcgccgt                             >  1:384750/1‑227 (MQ=255)
                                                                                                                                                                                                           gcgtaaaaaaaCTCCGCCCGCAGAAAGAAAACCCCGACATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  1:502923/223‑1 (MQ=255)
                                                                                                                                                                                                           gcgATAAAAACTTTCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  1:556112/228‑1 (MQ=255)
                                                                                                                                                                                                           gatgTAAAAAGCTCCGCCACAAAAAATAAAACCCCGCCATAGTAAAGAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATCCAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  2:486698/225‑1 (MQ=255)
                                                                                                                                                                                                                             acaaGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGt       <  1:150335/225‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaGATAACCCCGCCATAGTAAGGAGGCCTCCCTAATTCATCGGTATAACATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg  <  1:259094/228‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
TATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GGCGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACG  >  NC_000913/4065378‑4065826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A