Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F27 I0 R1 86 139.2 2835800 97.9% 2776248 226.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,151,329 T→G 23.8% A590A (GCA→GCC)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,151,3290TG23.8% 101.1 / 2.7 67A590A (GCA→GCC) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (34/12);  new base G (11/7);  total (46/21)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCGTTGTT  >  NC_000913/4151094‑4151565
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
ggTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCACTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCCTGAGGAGGtct                                                                                                                                                                                                                                            >  1:126694/1‑236 (MQ=255)
    gACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTG‑GACCACAGCGCCCCCTGCGCAGGGGggccg                                                                                                                                                                                                                                        >  2:1303247/1‑238 (MQ=255)
     aCATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGCGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCTCCTTTCAGGCTTCCTGGCGGTTTT‑GAAAGGACCGCCGCATGAGCAGGGGggccgc                                                                                                                                                                                                                                       >  1:1340019/1‑238 (MQ=255)
      cATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGcgccgccg                                                                                                                                                                                                                                     <  1:946423/239‑1 (MQ=255)
      cATCGCAGGAGATGACTGACAGTTCATCAATAATGCGAGGCCAGCTCTCTGTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTGAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCGTTCAGGCTGCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGcgccgcc                                                                                                                                                                                                                                      <  2:58767/238‑1 (MQ=255)
        tCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATCTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCcgcg                                                                                                                                                                                                                                   <  1:371563/239‑1 (MQ=255)
         cGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGGGCGCCGCcgcg                                                                                                                                                                                                                                   >  1:101826/1‑238 (MQ=255)
         cGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAG‑‑CTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCACGAGCCGGGGCGCCGCcgcgga                                                                                                                                                                                                                                 >  1:1054388/1‑236 (MQ=255)
              gagaTGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATAATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCACCCCCGCCTGAGCCGTGGCGCCGCCGGGACCAAt                                                                                                                                                                                                                             >  1:202000/1‑239 (MQ=255)
                        acAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTG‑TACAGCAACGCCGCCTGAGCAGGTGCGCCGCAGCCGACAAAGGAAccgcc                                                                                                                                                                                                                    >  2:1228706/1‑238 (MQ=255)
                            ttCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAAGCCGCCTTTCAGGCTTCCTGGCGGCTGC‑TACAACACCGCCGCACGAGAAGATGCGTCCCCCCCACCACTAGAACcgccgccg                                                                                                                                                                                                                >  2:786143/1‑236 (MQ=255)
                              cATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTT‑GACAGCAGCGCCGCATGAGCCAGGGCGCCGCCGCGACCAATGGAACCGCCGCGcccc                                                                                                                                                                                                             >  1:1237757/1‑238 (MQ=255)
                                    tAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGACTTTCAGGCTTCCTGGCGGGTGT‑GACAGCCGGGGCGGATGAGCCGGTGGGCCGCCGCCGACAAAGGAACCGCCGCGAGCGGGGaaa                                                                                                                                                                                                       >  2:113904/1‑238 (MQ=255)
                                       tGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGGGGaacaac                                                                                                                                                                                                    >  1:1171719/1‑239 (MQ=255)
                                           aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCACCGCCGCATGCGCAGGTGCGCCGCCGCGACCACTGGACACGCCGCGGCCGCGGCAACACGTCa                                                                                                                                                                                                >  2:29693/1‑239 (MQ=255)
                                                       tttCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGGATGAGCAGGTGCGCCGCCGCGACCAATGGAAACGCCCCCACCCGGGAACAACGTCCGCCCAAAAccc                                                                                                                                                                                     >  2:1378109/1‑238 (MQ=255)
                                                                      gggcggtgggTTGGCGTCCAGAGTCCCCCCGTTATAAAGGGGCGGTCTGCGGACGTGTTTTTCTG‑GCAGACCATTTTTAATGGGGATCACCTCGCCCTGGTCGGTTACGCGCGGGCCGCCTTTCAGGTTTCCTGGGGGTTGT‑GACAGCGGCGCCGCATGAGCAGGTGCCCCGCCGCGCCCATTGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGtt                                                                                                                                                                      <  2:596800/230‑1 (MQ=255)
                                                                              tgTTGGCTCCCAGAGCCGCCCGGCTAAAAAGCGACGGGGTGGGGTGGGTGTTTTCTG‑GCAGCCCCTATTAAAAGCTGATCATCTCGCCCTGTGCGGTTGCGCGCAGCCCCCCTTCCAGGCTTCCTGGCGGTGGC‑GACCGCAGCGCCGCAGGAGCGGGGGCGCCGCCGCGACCAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAAt                                                                                                                                                             <  2:889630/238‑1 (MQ=255)
                                                                                       ccAGAAGCCCCCCGTTATAAAGCGATAGGCGGGTGAGGGTGTTTTCGG‑GCGGCCCTTATTTAAAGCGCTTCATCCCGCCTTGTTCGGTTCCGCGCAGGCCGCCTTTCAGGCTTCGGGGGGGTTGT‑GACAGCAGCGCCCCATGGGCAGGGGCGCCGCCGCCACCAATGAAGCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCtgt                                                                                                                                                    <  2:1051564/239‑1 (MQ=255)
                                                                                         aGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCCGCGCCGCCTGGGCAGGGGCGCCGCCCCGACCAAAGGAACCGCCGCGGCCCTGGGACAACGCCAGCTCACTACCCCCCTTTTCCCAGGGTTTGTTTAATTCCTCCtgtg                                                                                                                                                   >  2:72028/1‑238 (MQ=255)
                                                                                                          aaggcacgggcggcTGA‑GGTTATTTCGGTGCAGAACTTATTCAAAGCGCCCCATCCCGCCTTTGCCGATTCCGCGCGCGCCGCCTTTCGGGTTGCCGGGCGGTAGT‑GCGAGCAGCCCCGTATGGGCGGGTGCCCGCCCGCACCCAATGAACCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAgg                                                                                                                                 <  2:938457/231‑1 (MQ=255)
                                                                                                               aCAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTCATATTGCGCCCCggca                                                                                                                               >  1:1394129/1‑234 (MQ=255)
                                                                                                                cgggCTGCGGGCGTTGTTTTCGG‑GCAGCCTTTTTTTAAGGGGTACCCTCGCCCCCTTTTGGTTTCCGCCCAGCCCGCCTTCCGGGCTTCCTGGGGTTTGT‑GACAGCGGCGCCGCATGAGCGGGTCCGCCCCCGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                            <  2:1126347/236‑1 (MQ=255)
                                                                                                                cAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTT‑GACAGCAGCGCCGCATGAGCAGGGGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                            >  1:1334210/1‑238 (MQ=255)
                                                                                                                cAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                            >  1:194610/1‑238 (MQ=255)
                                                                                                                cAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAAACGCCGCGCACGTGGAACAACGGCAGCTCAATACCCGCTTTTTCCCAAGTTTTGATTAATACATCCTGTGCCCTGTATTTCGCCCAGGAAGCt                                                                                                                            >  2:1207071/1‑238 (MQ=255)
                                                                                                                     tgctgACGGTGGTTTCTG‑GCAGACCATATTTAAAGCGTATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCCACCAATGGAACCGCCGCGCCCCTGGAACAACGTCAGCCCCATACCCCCCTTTTCGCAGGTTTTCATTAATGCATCCTGGGCCTGGTATTTCTGCCAGGAAGCGTCCAt                                                                                                                       >  2:1160170/1‑238 (MQ=255)
                                                                                                                       ctgACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATc                                                                                                                      >  1:1199554/1‑237 (MQ=255)
                                                                                                                              ttttttCTG‑GCCGACAATTTTCAAAGCGGCTCCCCCCGCCCTGTTGGTTTGCGCGCGCGCCCCTTTTCGGTCTTCGTGGGGGTTGT‑GCCACCGCCCCCACTGGAGGAGGGCCCCCCCCCCGACAAAGGTAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGc                                                                                                              <  2:1334210/235‑1 (MQ=255)
                                                                                                                              tgttttCTG‑GCATACCATATTAAAAGCGTCCCCCCCCGCCCTGTTCGTTTCCGCGCGGGCCCCCTTTCAGCCTGCCTGGCGGTTGT‑GACAGCAGCGCCGCAGGAGCGGGGGCGCCGCCGCGACCAATGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGc                                                                                                              <  2:194610/235‑1 (MQ=255)
                                                                                                                                 tttCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCCCCGCGACCGTGGGACAACGTCACCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCACGAAGCTGCCATCACTCCCGCATCt                                                                                                          >  2:1287046/1‑239 (MQ=255)
                                                                                                                                      g‑gCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCtttt                                                                                                       >  1:198583/1‑237 (MQ=255)
                                                                                                                                         cAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATg                                                                                                                                                            >  1:1030215/1‑182 (MQ=255)
                                                                                                                                           gACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGTCGGTTGT‑GACAGCAGCGCCGCCTGAGCAGGGGCGCCGCCGCGACCAATGGAACCGCCGCGCCCGTGGAACAACGTCCGCTCAATACCCGCTTTTTCGCATGTTTTGCTTAATCCCTCCTGCTCCTGATATTTCGTCCAGGGCACTTCCATCACTCCCGCATCTTTTTCTCAg                                                                                                 >  2:327242/1‑239 (MQ=255)
                                                                                                                                                           gATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGGGCCGCCGCGACCAATGGAACCGCCGCGGCCGTGGGACAACGTCAGCCCAATACCCGCTTTTTCGCAGGGATTGATTAATGCATCCTGTGCCTGATATTGCGACCAGGAAGCTGCAATCACTCCCCCATCTTTTGTCGGGTACGAATCGGCAatca                                                                                 >  2:325215/1‑239 (MQ=255)
                                                                                                                                                           gATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGGACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTACCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                 >  2:120491/1‑239 (MQ=255)
                                                                                                                                                            atcatcTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGGAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCCGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                 >  1:380892/1‑238 (MQ=255)
                                                                                                                                                            atcatcTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCCGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCTGCCATCACCCCCGCCTCTTTTGCTGCTGCGGAATACCCAatca                                                                                 >  2:1039839/1‑238 (MQ=255)
                                                                                                                                                            atcatcTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                 >  1:784876/1‑238 (MQ=255)
                                                                                                                                                            atcatcTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                 >  1:786479/1‑238 (MQ=255)
                                                                                                                                                                    gCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCGGCCATCACTCCCGCCTCTTTTGGTGAGTCGGGATAGCCAATCATCACCa                                                                           >  2:398153/1‑236 (MQ=255)
                                                                                                                                                                       cTGTTCGGTTACGCGCACGCCGCCTTTCGGGCTTCCGGGGGGTGGT‑AAGAGCGGCGCCGAAGGGGGAGGTGCGCCGCCGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGtt                                                                     <  1:261934/239‑1 (MQ=255)
                                                                                                                                                                         gTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGGGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGttt                                                                    >  2:394447/1‑238 (MQ=255)
                                                                                                                                                                          ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg                                                                   >  2:1293107/1‑238 (MQ=255)
                                                                                                                                                                          ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg                                                                   >  2:253957/1‑238 (MQ=255)
                                                                                                                                                                               ttACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGATGAGTCGGAATAGCCCATCATCCCCATCTGTTTGCCCTgc                                                             >  1:1014027/1‑238 (MQ=255)
                                                                                                                                                                                  cgcgCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGGGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTCCTGAGTCGGAAAAGCCAATCATCACCATCTGTTTGCCCTGCATc                                                          >  2:60029/1‑239 (MQ=255)
                                                                                                                                                                                   cccgcggCCGCCTCCCAGGCGTCGGGGCGGTTGC‑CACAGCGCCGCCGAGTGAGCGGGCGCGCCGCCGCACCAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCa                                                         <  2:1054388/234‑1 (MQ=255)
                                                                                                                                                                                    cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGGTTGCCCTGAATCAg                                                        >  2:705671/1‑239 (MQ=255)
                                                                                                                                                                                    cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTTCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAAACAg                                                        >  2:1271521/1‑239 (MQ=255)
                                                                                                                                                                                    cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGcc                                                                 >  1:977951/1‑230 (MQ=255)
                                                                                                                                                                                    cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGCGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCCGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGACATCACTCCCGCCTCTTTTGCTGAGTCGGAATATGCCATAATCACCATATGTTTGCCCTGAAtacg                                                        >  2:749779/1‑236 (MQ=255)
                                                                                                                                                                                    cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGG‑GACAGCAGCGCCGCATGAGCATGGGCGCCGCCGCGAACAATGGAACCGACGCGACCCTGGAACAACGTCAGCTCAATAACCGCTTTTTCGAAGGTTTTGATTAATGCAACCTGTGCCTGATATTGCGCCCAGGAAGATGCCATCACTCCCCCCTGTTTTGCTGCGTGAGGATAGCCAAACATCACCATCTGTTTGCCCTGAATCAg                                                        >  2:274557/1‑239 (MQ=255)
                                                                                                                                                                                       gggCCCCTTTCCAGCCCTCCGGGCGTTTGG‑GACGGCAGCCCCGCGAGAGCGGGTGCCCCGCCGCGCCCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGc                                                      <  1:749779/237‑1 (MQ=255)
                                                                                                                                                                                         gccgccTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCAc                                                   >  1:1075699/1‑239 (MQ=255)
                                                                                                                                                                                             ccTTTCAGGCTCCCGGGCGTTTGA‑GACCGGCGCCCCGCGTGAGCAGGTGCCCCGCCGAGACCAATGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                               <  1:673273/239‑1 (MQ=255)
                                                                                                                                                                                               ttccAGGCTCCCTGGCGGTTGT‑AACGGCGGCGCCCCATGGGAGGGGCCGCCGCCGCGCCCAAGGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATAcc                                             <  1:60029/236‑1 (MQ=255)
                                                                                                                                                                                                  ccggCTTCTGGGGGTTTGT‑GACAGCACCCCCGAAGGGGCGGGCGCCCCGCCGCGCAAAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                          <  1:786143/237‑1 (MQ=255)
                                                                                                                                                                                                   aGGCTCCCTGG‑GGTTGTGGACAGCGCCCCCGCAGGGGCGGGTCCCCCGCCCAGCACATGGAAACCGCCGCGACCGTGGAACAGCGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                          <  1:113904/238‑1 (MQ=255)
                                                                                                                                                                                                        tCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCCGTCAatat                                    >  1:368268/1‑239 (MQ=255)
                                                                                                                                                                                                        tCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCA‑CTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATAtt                                   >  1:1171110/1‑239 (MQ=255)
                                                                                                                                                                                                                 ttGT‑GACAGCCGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACCTCAGCTCAATACCCGCTTTTTTGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTTGGCCCAGGAAGCGGCCATCACTCCCGCATCTTTTGCTGAGTCGGACTAGCCAATCATCACCATCTGTTTGCCCTTAATCAGGCCACGATACCAGGCAATATTGcgca                              >  2:446570/1‑236 (MQ=255)
                                                                                                                                                                                                                    ggaaCAGCGGCGCCACATGGGCGGGGGCCCCGCCGCGACCAATGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTgg                         <  1:1378109/236‑1 (MQ=255)
                                                                                                                                                                                                                            accgccgcAGGGCCGGGGGCCCCGCGGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCAt                    <  1:1243984/234‑1 (MQ=255)
                                                                                                                                                                                                                             gcgcCGCATGAGGGGGTGCGCCGCCGCCACAAAGGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGAc                 <  1:398153/238‑1 (MQ=255)
                                                                                                                                                                                                                                       ggggggTGCCCCGCCGTGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCg       <  1:1207071/234‑1 (MQ=255)
                                                                                                                                                                                                                                        gCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCgtt     >  1:1025865/1‑239 (MQ=255)
                                                                                                                                                                                                                                           ggTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCgttgt   >  2:1409572/1‑238 (MQ=255)
                                                                                                                                                                                                                                             tGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCAt                                                                                                                       >  2:758479/1‑120 (MQ=255)
                                                                                                                                                                                                                                             tGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCgttgtt  <  1:1160170/237‑1 (MQ=255)
                                                                                                                                                                                                                                             tGCGCCGCCGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCAt                                                                                                                       <  1:758479/120‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            
GGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTG‑GCAGACCATATTTAAAGCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGT‑GACAGCAGCGCCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCGTTGTT  >  NC_000913/4151094‑4151565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A