Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 95 78.2 1797446 84.3% 1515246 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,280,577 A→G 22.9% S200G (AGC→GGC)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,5770AG22.9% 99.6 / 3.7 54S200G (AGC→GGC) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (5/35);  new base G (11/3);  total (16/38)
Fisher's exact test for biased strand distribution p-value = 1.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGA  >  NC_000913/4280342‑4280815
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
gttGCTTTCTCCGACCGATTCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAACAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAgct                                                                                                                                                                                                                                               <  1:393502/237‑1 (MQ=255)
ggTGCTTTCGCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAgc                                                                                                                                                                                                                                                <  1:693615/238‑1 (MQ=255)
     tttCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTCTCCCACGTGTTTGGGTGGgcgggctg                                                                                                                                                                                                                                          >  1:800047/1‑239 (MQ=255)
       tctcCGGACGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTa                                                                                                                                                                                                                                        <  1:226188/239‑1 (MQ=255)
            gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCTCCGGGTTTTTGGGTGTCTGGCTTTACGGt                                                                                                                                                                                                                                    >  1:628466/1‑238 (MQ=255)
               cgaTGCTTGTGCGTTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGtcgct                                                                                                                                                                                                                                <  1:139178/239‑1 (MQ=255)
                            ctctCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTTCTGCCGGGTTTTTGGGTGGCTGGCTGTACGGTGCCTCGCTGccgtttc                                                                                                                                                                                                                    >  1:371335/1‑232 (MQ=255)
                                    gATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGc                                                                                                                                                                                                            <  1:73360/238‑1 (MQ=255)
                                       tgttgGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGCCGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTCTGAAAGTGCCCATTGGCGGGATTTTTGCCCGGTTTGTGGGTGGCTTGCTGTACGCTCCGCCGCGGCGACTCTTCCGccccctgg                                                                                                                                                                                                        >  2:164146/1‑233 (MQ=255)
                                        tttgggtaaGGGGGCATCCCTAAAACAATTGTGGGCATTTTGCAGGGCGAAG‑GTTGTATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGGTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTgggg                                                                                                                                                                                                       <  2:243830/232‑1 (MQ=255)
                                         ttgGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGCATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTTTGGTGGGATGGCTGTACGGTCGCTCGCTGCGCTTCCTCAGCCGCTGGGGc                                                                                                                                                                                                      >  1:97883/1‑239 (MQ=255)
                                           ggCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTTTCCCCGGGTTTTTGGTGGGCTGGCTTTCAGGTCGCCCCCTCCGATTCTCCAGCCCCTCgggggg                                                                                                                                                                                                    >  1:78986/1‑239 (MQ=255)
                                              gAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGGCGAATTTATGAAAGTAGCCATTGGCGGCATTCTCGGCGGTTTTGTTGTGGGCTGGCGGTTAGGGCGCTCGCTTCGGTTCCCCAGCCGCTGGGGCgggtg                                                                                                                                                                                                 >  1:12707/1‑236 (MQ=255)
                                                 gggCGCATCCCGAAAAAAATCATGGGCATTTGGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTTGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGtgatg                                                                                                                                                                                               <  1:765366/238‑1 (MQ=255)
                                                      cATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCCCCGGCTTTTTGGGGAGCGGGCTGTACGGTCGCTCCCTCCGCTTTCTCAGCCGCTGGGGCGGGGGTGAAaccc                                                                                                                                                                                         >  1:620206/1‑238 (MQ=255)
                                                      cATCCCGAAAAAAATCAGGGGCTTTGGGGAGGGGGAAGGGTTG‑AAGAATGACGCCTCCGGTCTGGTTTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTGCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCcg                                                                                                                                                                                         <  2:751466/239‑1 (MQ=255)
                                                             aaaaaaaTCATGGGCATTTTGCAGGGCGAAGGGTTG‑ATGAATGACGCCTCCGGCCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGTGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCa                                                                                                                                                                                 <  1:198106/239‑1 (MQ=255)
                                                                    tCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTc                                                                                                                                                                          <  1:617881/239‑1 (MQ=255)
                                                                     tgtGGGTATTTTGGGGGGCGAAGGGTTG‑GAGAATGCGGCCTCCGGTCTGGTGTCGTTGAAGGTTGCGGGGCCAGTGGCGATGGGGACGATGATCTTCACCGTCGGGGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTcc                                                                                                                                                                         <  2:619487/237‑1 (MQ=255)
                                                                              tttGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCCCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CCCAGATCGTCCTGCTGtt                                                                                                                                                                  >  1:440033/1‑237 (MQ=255)
                                                                                         gagggTTG‑GTTAATGCCGCCCTCGGTCGGGTTTTGTTGAAGTTTGCCGTAGCAGGGGGCATGGGGAAGATGATCTTCACCGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTc                                                                                                                                                    <  2:789549/235‑1 (MQ=255)
                                                                                         aaGCGTTG‑ATGAATGACGCGTCCGGGTTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑tt                                                                                                                                                     <  1:872127/238‑1 (MQ=255)
                                                                                                       tggcGCCTCCGGTTTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGa                                                                                                                                       <  1:682249/236‑1 (MQ=255)
                                                                                                       tggcGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGa                                                                                                                                       <  1:218473/236‑1 (MQ=255)
                                                                                                       tgaCGCCTCCGGTCTGGTTTCGTTGAAGTTGGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGa                                                                                                                                       <  1:240779/239‑1 (MQ=255)
                                                                                                            ccgccGGTGTGGTTTCGTGGAGGTTTGCCGTAGCAGTGGGGGTGGGGGCGATGATCTTCACCGTCGGGGGTGGGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGcc                                                                                                                                  <  2:571145/236‑1 (MQ=255)
                                                                                                              tCCGGTTGGGTTTCGTTGAGTGTTGCCGTGGAGGGGGGGATGGGGCAGTTGGCCTCGCCCGTGGGGGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGa                                                                                                                                <  2:455045/239‑1 (MQ=255)
                                                                                                              gccGGTCTGGTGGCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGa                                                                                                                                <  1:738466/238‑1 (MQ=255)
                                                                                                                       gtgtCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCCTTGGCGGTATTCTCGCCGGTGTTGTGGTGGGCTGGCTGTACCGTCGCTCGCTGCGATTTCCCAGGCGCTGGGGGGGGGGTGAACCCCCCG‑CGCCGCTCCGCCCGCTGTTCTCGCTGCCA‑TTCGCCTCTTCTTTGATTGcccgaccaacttg                                                                                                                       >  2:504212/1‑228 (MQ=255)
                                                                                                                             ttGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGTGGGCTGGCTGGACGGTCGCCCGCTGCGGTTCCTCAGCCGCTGGGGCGGGGATGAACCCGCGA‑CGCCGATCGTCCCGCTGTTCCTGCTGCCA‑TTCCCTTCTTTTCTGATTTCCGGACAAATTgggggtt                                                                                                                  >  1:236412/1‑233 (MQ=255)
                                                                                                                                     tGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGCATTCTCGCCGGGTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCCGCCGCTGGGGCGGGGGTGAACCCCCGA‑CCCAGATCGTCCTTCTGTTCCTCCCGCCC‑TTCCCCTCTTATTTGATTGCCCGACAAATTGGGGTTTCCGGGATcc                                                                                                         >  2:429620/1‑239 (MQ=255)
                                                                                                                                            gCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGGGGACTGGCTGTACGGGCCCTCCGTCGCATTCCTCAGCCGCTGGGGCGGGGGTGAACCCCCGAGCGC‑GAGCGGCCCGCCGTTCCTGCCGCCATTTCCCTCTTTATC‑GATTGCCGAAAAAATTTGCGTTTCCCGcccccccccgcg                                                                                                  >  2:817064/1‑228 (MQ=255)
                                                                                                                                                    gttGGGGACGTTGTTCTTTCCCGTCGGGGGTGGGACGGTCGAATTTATGAAAGTGGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCACCCTCGCTGCGGTTgccg                                                                                          <  2:236412/237‑1 (MQ=255)
                                                                                                                                                           aCGTTGATCTTCCCCTTCGGGGTTGGGGCGGTCGAATTTATGAAATTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGa                                                                                    <  2:820318/238‑1 (MQ=255)
                                                                                                                                                            cGATGATCTTCCCCGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGGCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGTATCCTCGCTGCGGTTGCCGCCGGGAt                                                                                   <  1:802704/238‑1 (MQ=255)
                                                                                                                                                            cGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTTTGGGGAGCTGGCTGTAAGGTCGCTCCCTGCGGTTCCTCAGCCGCTGGGGCGGGGGGGAACCCCCGC‑GGCGCACCGTCCGCCTTTTCCTCCGGCCA‑TTCGCTTCTTATCTGATTTCCGGAAAAATTTGGGTTTTCCGCATCCCCCCTCCGGTTTCCCCCgggtgg                                                                                  >  2:210067/1‑236 (MQ=255)
                                                                                                                                                                 atCTTCCCCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGGACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGAcc                                                                               <  1:28378/237‑1 (MQ=255)
                                                                                                                                                                     tcgccGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCCCGCCGGTTTTGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCAcc                                                                         <  1:164146/236‑1 (MQ=255)
                                                                                                                                                                     tCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTGGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCAcc                                                                         <  1:226730/239‑1 (MQ=255)
                                                                                                                                                                          gggggcgtTGGGACGGGCGAATTTTTGAAGGGTGCCTTTGGTGGTATTCTCGCCGGTGTTGTGGTGAGCGGGCTGTACGGGCGCTCGGTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGGGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑GTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTc                                                                    <  2:568097/236‑1 (MQ=255)
                                                                                                                                                                            cggcggTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCCCCGGATTTGTGGTGGGCTGGCTGTACGGGTCCTCGCTGCGGTTCCTCCGCCGCTCGGGGGGGGGTGGACCCGCGC‑CGCCGAACGTCCCGCTGTTTCTGGTTCCC‑TTTCCCTCTTTTTTGCTTGCCCCACCAATTTGCGTTTCCGGCACCCCCCCCGCGGTTGCCGCCGGGGGGAAAATCACCCCCTCCg                                                                  >  2:392338/1‑239 (MQ=255)
                                                                                                                                                                                  ttcGAGGGTTGAAGTTATGCAAGGCGCCATTGGCGGTAGTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCCCGCTGCGATTCCTCAGCCGCTGGGTCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGtgtg                                                             <  1:883354/236‑1 (MQ=255)
                                                                                                                                                                                    tcaCGGTGGAATTTTTTAAAGTGGCCCTTGGGGGTTTTCTGGCCGGTTTTGGGGGGGGGTGGCTGGACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAt                                                           <  2:628466/236‑1 (MQ=255)
                                                                                                                                                                                    ggctgttggAATTTTTGGTAGTGGCTGTTGGCGGTTTTCTCGCCGGTTTGGTGGGGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑GGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAtg                                                          <  2:78986/231‑1 (MQ=255)
                                                                                                                                                                                    cGACGGTGGAATTTATGAAAGTAGCCATGGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAt                                                           <  1:31851/238‑1 (MQ=255)
                                                                                                                                                                                          tCGAATTTATGAAAGGAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCACCACCAGCGccagcgcg                                                             <  1:286635/230‑7 (MQ=255)
                                                                                                                                                                                                  aTGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCt                                            <  1:244296/239‑1 (MQ=255)
                                                                                                                                                                                                   tGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGTGAGGTGGCTGTAAGGTCGCTCGCTTCGATTCCTCAGCCGCTGGGGCGGGGGTGAACCCCCCA‑CGCAGATAGGCCTGCCGTTCCTGCTTCCA‑TTCGCTTCTTTTCTGCTTGACGCACCTATTTGCGTTTCCGGCATTCTCCCTGAGGGTTCCTCCGGGACGACCCTCCACCCCGCCCGGGTGAagcgccgggtgcccccg                                           >  2:136908/1‑235 (MQ=255)
                                                                                                                                                                                                                   gggTTTTCCCGCCGGTTTGGTGGGGGGCCGGCGGTGCGGGCCGTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGt                            <  2:571357/237‑1 (MQ=255)
                                                                                                                                                                                                                     gttttttcGCCGGTTTTTGGGTGGGCGGGGTGTCCGGTCGCTCCTCGCTTTTCCTCCGCCGCTGGGGGGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCGGCCA‑TTCGCGTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCa                         <  2:581353/233‑1 (MQ=255)
                                                                                                                                                                                                                       aTTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGGTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:210067/239‑1 (MQ=255)
                                                                                                                                                                                                                       aTTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:85433/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttCCCGCCGGTTTTGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCCGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:688625/238‑1 (MQ=255)
                                                                                                                                                                                                                                   tttGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTggg            <  1:136908/238‑1 (MQ=255)
                                                                                                                                                                                                                                            aGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGGGCAAACAGCACCTGGGCGATGCTGGa  >  1:53268/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
GGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG‑ATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGA‑CGCAGATCGTCCTGCTGTTCCTGCTGCCA‑TTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGA  >  NC_000913/4280342‑4280815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A