Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F999 I1 R1 225 156.4 11709729 83.8% 9812752 75.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,765 +G coding (476/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,7641.G99.2% 342.1 / ‑3.8 125coding (477/1764 nt)dgoA2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (58/66);  total (58/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 131 total aligned reads displayed.

TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG  >  NC_000913/3870689‑3870834
                                                                             |                                                                      
ttGCCCGGCGCGATAGCAATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑t                                                                         <  1:731311/76‑1 (MQ=255)
 tGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑g                                                                       <  1:3591457/76‑1 (MQ=255)
 tGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑g                                                                       >  2:4546810/1‑76 (MQ=255)
  gCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑gg                                                                      >  1:255656/1‑75 (MQ=255)
   cccGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑ggc                                                                     >  1:470313/1‑74 (MQ=255)
    ccGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑ggcg                                                                    <  1:2304156/76‑4 (MQ=255)
       gcgcGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCa                                                                  >  1:2401292/1‑76 (MQ=255)
        gccgATAGAGGCCCCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc                                                                 <  2:243631/74‑1 (MQ=255)
        gccgATAGAGATCGCGCCATGAGCCCGCCTCTGTGCAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc                                                                 <  1:831113/74‑1 (MQ=255)
        cgcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAc                                                                 <  1:5630502/76‑1 (MQ=255)
         gcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcg                                                                <  1:2428346/76‑1 (MQ=255)
         gcgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcg                                                                >  1:1071826/1‑76 (MQ=255)
          tgATAGAGATCGGTTCCTAATCCCGCCCCTGCACAACCTCCGTCTATCCACGGCGCCAGGTTTTC‑TGGCGTCAcgc                                                               <  1:5050975/75‑1 (MQ=255)
          cgATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGCTTTC‑TGGCGTCAcgc                                                               >  2:2937933/1‑76 (MQ=255)
           gATAGGGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgcc                                                              <  2:5082564/76‑1 (MQ=255)
           gATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgcc                                                              <  1:491380/76‑1 (MQ=255)
             tAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAcgccgc                                                            <  1:253251/76‑1 (MQ=255)
              agagATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACgccgcc                                                           <  1:2268710/76‑1 (MQ=255)
              agagATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACgccgcc                                                           <  1:1621073/76‑1 (MQ=255)
               gagaTCGCTGCCTACGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa                                                          <  2:4434132/76‑1 (MQ=255)
               gagaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa                                                          >  2:2806886/1‑76 (MQ=255)
               gagaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCa                                                          >  2:4853596/1‑76 (MQ=255)
                agaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCaa                                                         >  1:1691182/1‑76 (MQ=255)
                agaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTCC‑TGGCGTCACGCCGCCaa                                                         >  1:5192540/1‑76 (MQ=255)
                 gaTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAAc                                                        >  2:635103/1‑76 (MQ=255)
                  aTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACg                                                       <  1:4870148/76‑1 (MQ=255)
                  aTCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACg                                                       >  1:2158220/1‑76 (MQ=255)
                    cGCTGCCTACGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGc                                                     <  2:1146927/76‑1 (MQ=255)
                    cGCTCCCTACGCCCCCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGc                                                     <  1:1263148/76‑1 (MQ=255)
                       tGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCCCCAACGGCaaa                                                  >  2:766977/1‑76 (MQ=255)
                        gCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAg                                                 >  1:5698598/1‑76 (MQ=255)
                         ccTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGa                                                <  2:4589678/76‑1 (MQ=255)
                          cTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGAc                                               <  1:1015185/76‑1 (MQ=255)
                           tacgccggcccCTGCACAGCCTGCGTCTATCCACTGCGCCAGGTTTTC‑GGGCGTCACGCCGCCAACGGCAAAGACt                                              <  2:508290/73‑1 (MQ=255)
                           tAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACt                                              <  2:867254/76‑1 (MQ=255)
                           tAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACt                                              <  1:1258814/76‑1 (MQ=255)
                             accccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            <  1:5237484/74‑1 (MQ=255)
                             accccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            <  1:1018905/74‑1 (MQ=255)
                             aGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            <  1:3564262/76‑1 (MQ=255)
                             aGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            <  2:5511589/76‑1 (MQ=255)
                              gcccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCg                                           >  1:5363068/1‑76 (MQ=255)
                                ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            <  2:2357273/73‑1 (MQ=255)
                                ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGc                                            >  1:2357273/1‑73 (MQ=255)
                                ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                         >  2:4566318/1‑76 (MQ=255)
                                ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                         <  1:2343182/76‑1 (MQ=255)
                                ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                         <  2:1933154/76‑1 (MQ=255)
                                 cgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCACAGACTCCGATg                                        >  2:5260132/1‑76 (MQ=255)
                                  gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt                                       >  2:4726132/1‑76 (MQ=255)
                                  gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt                                       >  1:3332671/1‑76 (MQ=255)
                                  gcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt                                       >  1:3272640/1‑76 (MQ=255)
                                  gcccCTCCACATCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt                                       <  1:5016922/76‑1 (MQ=255)
                                   ccccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCCACGGCAACGACTGCGATgcc                                      >  2:227879/1‑74 (MQ=255)
                                    cccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                     <  2:1733024/76‑1 (MQ=255)
                                       tGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGag                                  >  1:810253/1‑75 (MQ=255)
                                        gcccaACCGGCGTCTACCCACTGCGCCAGGTTTTC‑TGGCGGCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg                                 <  2:5385071/73‑1 (MQ=255)
                                        gCACACGCTGCGTCTATCCTCTGCGCAGGATTTTCGG‑GCTCCACGCCGCCAACGGCAAAGACTTCGATGTCCGATg                                 <  1:5219467/76‑1 (MQ=255)
                                        gCACAACCGGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg                                 <  2:3394060/76‑1 (MQ=255)
                                         cacaACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATgg                                <  1:3705313/76‑1 (MQ=255)
                                          acaACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGc                               >  2:2211461/1‑76 (MQ=255)
                                            atccTGCGTCTGGCCCCTGGGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCaa                             <  2:671032/74‑1 (MQ=255)
                                             aCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAAt                            <  1:2333255/76‑1 (MQ=255)
                                              ccTGCGTCTGTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGGCCGATGGCAATa                           <  1:4800355/76‑1 (MQ=255)
                                              ccTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCACAGACTGCGATGTCCGATGGCAATa                           >  1:1179477/1‑76 (MQ=255)
                                                 gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg                        <  1:3707530/76‑1 (MQ=255)
                                                 gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg                        >  1:4294265/1‑76 (MQ=255)
                                                 gCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg                        <  1:4566319/76‑1 (MQ=255)
                                                   gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt                      >  1:2222771/1‑76 (MQ=255)
                                                   gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt                      <  2:2401292/76‑1 (MQ=255)
                                                   gTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt                      <  1:3737933/76‑1 (MQ=255)
                                                     cTATACACTGCGCCAGGTTTTC‑TGGCGTAACGCCGCCAACGGCAAAGACTGCGATGGCCGATGGCAATACCGCttt                    >  1:2491287/1‑76 (MQ=255)
                                                      tagccACTGCGCGAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCtttt                   <  2:742742/73‑1 (MQ=255)
                                                       agccACGGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTACGATCGCAAGACCGACTTTa                  >  2:4268270/3‑76 (MQ=255)
                                                       aTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAACGACTGCGATGTCCGATAGCCATCCCGCTTTTa                  >  2:604124/1‑76 (MQ=255)
                                                       aTCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTa                  <  1:4097674/76‑1 (MQ=255)
                                                        tCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                 >  1:3007504/1‑76 (MQ=255)
                                                        tCCACGGCGCCGGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                 <  2:4333812/76‑1 (MQ=255)
                                                         ccACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAc                >  1:4286121/1‑76 (MQ=255)
                                                         ccACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCCTTTAAc                >  2:1238261/1‑76 (MQ=255)
                                                          cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg               >  2:4694729/1‑76 (MQ=255)
                                                          cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg               >  1:3826759/1‑76 (MQ=255)
                                                          cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg               <  2:4186274/76‑1 (MQ=255)
                                                          cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg               >  2:3453629/1‑76 (MQ=255)
                                                          cACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg               <  1:5040694/76‑1 (MQ=255)
                                                           aCTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGc              >  1:3042330/1‑76 (MQ=255)
                                                           aCTGCGCCAGGGTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGCTGACAATACCGCTTTTAACGc              >  1:4319549/1‑76 (MQ=255)
                                                             tGCGCCAGGTTTTC‑TGGCGTTACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTTACGCtt            >  1:3118419/1‑76 (MQ=255)
                                                              gcgcCAGGTTTTC‑TGGTGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt           <  1:4554768/76‑1 (MQ=255)
                                                              gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt           >  1:68581/1‑76 (MQ=255)
                                                              gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt           >  2:297282/1‑76 (MQ=255)
                                                              gcgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt           <  1:4539934/76‑1 (MQ=255)
                                                               cgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTg          <  2:1718144/76‑1 (MQ=255)
                                                               cgcCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACCCTTTg          >  1:2372458/1‑76 (MQ=255)
                                                                  cAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATg       <  1:407257/76‑1 (MQ=255)
                                                                   gggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt      <  2:2793747/75‑1 (MQ=255)
                                                                   aGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt      >  2:5530307/1‑76 (MQ=255)
                                                                    ggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa     <  1:1583287/76‑1 (MQ=255)
                                                                    ggTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa     >  1:1250291/1‑76 (MQ=255)
                                                                     gTTTTC‑TGGCGTCACGCCGCCAACGGCAAAAACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt    <  2:4717534/76‑1 (MQ=255)
                                                                      ttttC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt   <  2:2261922/76‑1 (MQ=255)
                                                                       tttC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTg  >  1:96966/1‑76 (MQ=255)
                                                                             |                                                                      
TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG  >  NC_000913/3870689‑3870834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A