Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,960,578 |
2 bp→CG |
coding (1266‑1267/1485 nt) |
gppA ← |
guanosine pentaphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,960,578 | 0 | G | C | 100.0%
| 354.3
/ NA
| 132 | L423V (CTC→GTC) | gppA | guanosine pentaphosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (60/72); total (60/72) |
| * | NC_000913 | 3,960,579 | 0 | C | G | 99.2%
| 363.3
/ ‑5.0
| 132 | R422R (CGG→CGC) | gppA | guanosine pentaphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (59/72); minor base A (1/0); total (60/72) |
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
Only 100 of 132 total aligned reads displayed.
CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA > NC_000913/3960509‑3960646
||
cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcgg > 1:1539593/1‑76 (MQ=255)
gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggg > 1:5365395/1‑75 (MQ=255)
gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:307086/76‑1 (MQ=255)
gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:3783056/76‑1 (MQ=255)
gagaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc < 1:4702144/76‑1 (MQ=255)
agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggcg < 2:2761647/76‑1 (MQ=255)
aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt > 2:3947458/1‑76 (MQ=255)
aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt < 2:5000075/76‑1 (MQ=255)
aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt < 1:671074/76‑1 (MQ=255)
tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa > 1:513224/1‑76 (MQ=255)
cGTCACGGCGACGGCTGTCAAAAATGATGTCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc < 1:5583435/76‑1 (MQ=255)
gTCGCGTCGTCGGCTGGCAAAAATGCTGGCGAGGCGTAGTAAAGTGCCGAGTTGTTCTGCGACGCGCGGCGGTTCg < 2:2245476/76‑1 (MQ=255)
gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg > 2:3867731/1‑76 (MQ=255)
gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACg > 2:1710550/1‑76 (MQ=255)
tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg > 1:1578354/1‑76 (MQ=255)
cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc > 2:805927/1‑76 (MQ=255)
cACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc < 1:319276/76‑1 (MQ=255)
aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa < 2:3013182/76‑1 (MQ=255)
cGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGcct > 2:4503362/1‑74 (MQ=255)
cGGCGACGGCGGGCAAAAATGATGGCCAGGCGTAGTAAACGGCACAGGTGTTCAGAGACGCGCGGCGGCACgggct > 2:4372951/1‑73 (MQ=255)
ggCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt > 1:2800957/1‑76 (MQ=255)
cGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtttt < 1:4601176/76‑1 (MQ=255)
gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 1:3610882/1‑76 (MQ=255)
gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 2:937449/1‑76 (MQ=255)
gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg < 1:3305082/76‑1 (MQ=255)
gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTg > 1:2317126/1‑76 (MQ=255)
aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc > 1:90022/1‑76 (MQ=255)
aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc < 1:5077018/76‑1 (MQ=255)
aCGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc < 1:4412451/76‑1 (MQ=255)
ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:5649437/1‑76 (MQ=255)
ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:4903114/1‑76 (MQ=255)
ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTg > 1:5821789/1‑76 (MQ=255)
gCTGGCAAAAATGAGGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa < 2:5267732/76‑1 (MQ=255)
gtGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt < 2:5036343/75‑1 (MQ=255)
cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt < 2:4475729/76‑1 (MQ=255)
cTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAt > 1:2072488/1‑76 (MQ=255)
gggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg < 1:347259/75‑1 (MQ=255)
ggCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc > 1:7654/1‑76 (MQ=255)
gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa > 1:2330830/1‑76 (MQ=255)
gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa > 2:1907435/1‑76 (MQ=255)
gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa < 2:513224/76‑1 (MQ=255)
aaaagTGATGTGCAGGCATAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc < 1:4372952/76‑1 (MQ=255)
aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa < 2:4353805/76‑1 (MQ=255)
aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa > 2:2339938/1‑76 (MQ=255)
aaaTGAGGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa < 2:5748751/76‑1 (MQ=255)
aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCCTTTTGCCGCTGCAGCgct > 2:588078/1‑74 (MQ=255)
aaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAt < 1:5466917/76‑1 (MQ=255)
aaTGATGGCCAGGAGAAGAAAACGGCAGAGGTGTTGCGCGACGCGCGGCCGCCGGGGATGTTGGTGATGCAGCgct > 1:3222460/1‑74 (MQ=255)
atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg > 2:2234423/1‑76 (MQ=255)
atgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATg < 2:2152991/76‑1 (MQ=255)
gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag < 2:5063302/76‑1 (MQ=255)
gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgag < 1:461051/76‑1 (MQ=255)
tgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag > 1:2888159/1‑76 (MQ=255)
tgGCCAGGCGTAGGAAAGGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagag > 1:2594137/1‑76 (MQ=255)
ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga > 1:481656/1‑76 (MQ=255)
ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga < 2:880839/76‑1 (MQ=255)
gcgaGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTTCGGCATTTTGCTGATGCAGCGGTGAGAGAt < 2:3718830/73‑1 (MQ=255)
ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 1:3155168/76‑1 (MQ=255)
ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 1:1967425/76‑1 (MQ=255)
ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc < 2:1421098/76‑1 (MQ=255)
cAGGCGTGGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg < 2:2929369/76‑1 (MQ=255)
cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGCGAGATCg > 1:4171072/1‑76 (MQ=255)
cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCg < 2:3556064/76‑1 (MQ=255)
cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGGACGGCATTTTGCTGATGCAGGGGTGAGAGATCg > 2:5633442/1‑76 (MQ=255)
aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa > 1:563759/1‑76 (MQ=255)
aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa > 1:1741278/1‑76 (MQ=255)
aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa < 1:4338733/76‑1 (MQ=255)
aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCCATGATAGATCGa > 1:1781389/1‑76 (MQ=255)
ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAc < 1:2558986/76‑1 (MQ=255)
gCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc < 1:4184222/76‑1 (MQ=255)
cGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCCTTTTGCTGATGCAGCGATGAGAGATCGACCg > 2:1477573/1‑76 (MQ=255)
gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg < 1:1536238/76‑1 (MQ=255)
gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCgg > 1:2679742/1‑76 (MQ=255)
gtagtaAACGGCAGAGGTGTTCTGCGACGCGCGGAGGAACGGGAGTTTGCCGATGCAGCCGTGCGAGATGGATCgg > 1:3491763/1‑76 (MQ=255)
tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGa < 1:5259213/76‑1 (MQ=255)
gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt > 1:1007872/1‑76 (MQ=255)
gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAtt < 2:4227331/76‑1 (MQ=255)
aaaCGGCAGAGTTGTTCTGCGGCGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAg < 2:549137/76‑1 (MQ=255)
aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt < 2:1007872/76‑1 (MQ=255)
aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt > 2:4717175/1‑76 (MQ=255)
aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc > 1:491899/1‑76 (MQ=255)
aCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTc < 1:3947459/76‑1 (MQ=255)
aCGGCAGAGTTGTTCTGCGACGCGCCGCGGCAAGGCACCTTGCTGATGCAGCGATGGGAGGTCGATCGGGATAGTc > 1:209492/1‑76 (MQ=255)
cGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt < 2:2305579/76‑1 (MQ=255)
ggCAGAGTTGTTCTGCGACACGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg > 1:4095266/1‑76 (MQ=255)
gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTgg < 2:2399727/76‑1 (MQ=255)
cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGt < 2:3491763/76‑1 (MQ=255)
agagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt < 2:2631798/76‑1 (MQ=255)
gagTTGTTCTGCGACGCGCGGCGTTACGGCATTTTGCTGATGCAGCGATGAGAGATCGGCCCGCTTAGTCTgggcg > 2:482071/1‑73 (MQ=255)
gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCCGCGATGAGAGATCGGTCGGATTAGTCTGGTTg < 1:589075/76‑1 (MQ=255)
gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGCGATCGCCCGGATTAGTCTGGTTg > 2:110424/1‑76 (MQ=255)
gagTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg < 2:2038296/76‑1 (MQ=255)
agTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTgg > 2:4755612/1‑75 (MQ=255)
gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAg > 2:4313861/1‑76 (MQ=255)
ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGc < 1:5106050/76‑1 (MQ=255)
tgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa < 1:2241529/76‑1 (MQ=255)
gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg < 1:2489041/76‑1 (MQ=255)
ttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGTGCAGt > 2:3835414/1‑76 (MQ=255)
tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa < 2:1848203/76‑1 (MQ=255)
tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa < 1:4503363/76‑1 (MQ=255)
||
CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA > NC_000913/3960509‑3960646
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
