Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,969,103 |
2 bp→GC |
coding (232‑233/1263 nt) |
wecC → |
UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,969,103 | 0 | C | G | 99.3%
| 401.9
/ NA
| 148 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (79/68); minor base T (0/1); total (79/69) |
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 3,969,104 | 0 | G | C | 98.6%
| 374.1
/ NA
| 143 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (73/67); minor base A (2/0); total (75/68) |
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 148 total aligned reads displayed.
CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG > NC_000913/3969035‑3969171
||
tgAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct > 2:553365/2‑76 (MQ=255)
ggAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct < 2:5324035/75‑1 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 2:2443621/1‑76 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:2671335/1‑76 (MQ=255)
ggtgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa < 2:4329810/75‑1 (MQ=255)
tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:2045513/76‑1 (MQ=255)
tagtaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:2794505/76‑1 (MQ=255)
tagtaAAACCGGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:179493/76‑1 (MQ=255)
tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:5450704/76‑1 (MQ=255)
agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 1:1375061/1‑76 (MQ=255)
agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 2:2001420/1‑76 (MQ=255)
taAAACCTGCCGGAGAAGGCGGTGTTTTACGAGGGAGCAGGACGCCGGTTGAAGCGGATGCCTGGCTGATTGCTGt < 2:1035919/76‑1 (MQ=255)
aaaacCTGCCGTAGAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa < 2:2157772/76‑1 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:3256200/1‑76 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 2:2288772/1‑76 (MQ=255)
aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc > 1:5470265/1‑76 (MQ=255)
ccTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa < 2:1315850/75‑1 (MQ=255)
aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa < 1:553365/76‑1 (MQ=255)
cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc < 1:5451150/76‑1 (MQ=255)
cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc > 2:5336222/1‑76 (MQ=255)
tGCCGTGGAGGGCGTTTTTTTACGAGGGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg < 1:3470525/76‑1 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:803433/1‑76 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:4629425/1‑76 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGACGCCTGGCTGGCTGATGTAGCCACg > 1:2357829/1‑76 (MQ=255)
gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc < 2:3257876/76‑1 (MQ=255)
gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc < 1:476922/76‑1 (MQ=255)
gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc > 1:5575484/1‑76 (MQ=255)
ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc > 1:3420599/1‑76 (MQ=255)
cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg > 2:4143753/1‑76 (MQ=255)
gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 2:3238406/1‑76 (MQ=255)
aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGctt > 2:1165153/1‑76 (MQ=255)
gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa < 2:3965360/76‑1 (MQ=255)
aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGGCGTTTaa > 2:338555/1‑76 (MQ=255)
aGGCGGTTTTTTACGAGCGAGCACGACGCCCGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg > 2:2458665/1‑76 (MQ=255)
ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg < 2:3059087/76‑1 (MQ=255)
ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg < 1:108980/76‑1 (MQ=255)
gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 2:428528/76‑1 (MQ=255)
gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg > 1:4489204/1‑76 (MQ=255)
gCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCGGGCTGATTGCTGTACCCACGCCGGTGAAggg < 2:471446/76‑1 (MQ=255)
gggTTTTTTACGAGCGGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc < 2:3752341/75‑1 (MQ=255)
cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc > 1:3886330/1‑76 (MQ=255)
ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:3437174/1‑76 (MQ=255)
ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:4641953/1‑76 (MQ=255)
ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt < 1:2870789/76‑1 (MQ=255)
ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt < 2:5696443/76‑1 (MQ=255)
tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGCTTGCTGTACCCACGCCGTTTAATGGCGATc > 1:4977345/1‑76 (MQ=255)
tttttACGAGCGAGCACGACGCCAGCTGAAGAGGATGACCGCACGATTCCCGCACCCACGCCGTCTAAGcgcgccc > 2:336295/1‑73 (MQ=255)
tttgACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa < 2:568519/76‑1 (MQ=255)
ttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg > 1:2828138/1‑76 (MQ=255)
tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa > 2:2917693/1‑76 (MQ=255)
tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 1:1348197/76‑1 (MQ=255)
gaagagcgaggAGGGCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 2:5579527/73‑1 (MQ=255)
aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg > 2:2709203/1‑76 (MQ=255)
aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 1:1111474/76‑1 (MQ=255)
aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 2:4236554/76‑1 (MQ=255)
cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc < 1:5336223/76‑1 (MQ=255)
gagcgagcACGACGCCAGTAGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 2:3351301/76‑1 (MQ=255)
cgcgggcACGCCGCCATTTGAAGCGTATTGCTGGCTGCTTGCTGTCCCCACGCCTTTTACGGCCGATCATGAGCCa < 1:4739976/75‑1 (MQ=255)
agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa > 2:5258890/1‑76 (MQ=255)
agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa > 2:2678208/1‑76 (MQ=255)
gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg < 1:4396605/76‑1 (MQ=255)
gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg > 1:652473/1‑76 (MQ=255)
cgagcACGACGCCAGTTGAAGCGGATGCCTGGCCGATTGCGGTACCCACGCCGTTCAAGGGCGATCACGAGCCAGa > 2:1080326/1‑76 (MQ=255)
gagcAGGACGCCAGTTGAAGCGGATGCCTGGCTGATTGATGGAGCCACGCCGTTTAAGGGCGCTCATGAGCGAggt > 1:1186429/1‑74 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 1:1570429/76‑1 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 1:550377/76‑1 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:835252/1‑76 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:1847321/1‑76 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 2:540152/76‑1 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:63599/1‑76 (MQ=255)
gagcACGAAGCCAGTTGAAGCGCATGCCAGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAGCATGAGGCAGat > 2:3516766/1‑76 (MQ=255)
agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 1:2001420/76‑1 (MQ=255)
gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat < 2:2562011/76‑1 (MQ=255)
cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 1:3712417/76‑1 (MQ=255)
cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGGTTAAGGGACATCATGAGCCAGATATg > 2:1190103/1‑76 (MQ=255)
acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa < 1:2288772/76‑1 (MQ=255)
acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa > 2:1659336/1‑76 (MQ=255)
cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc > 1:4164764/1‑76 (MQ=255)
gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 2:1312223/73‑1 (MQ=255)
acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:195279/76‑1 (MQ=255)
acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:1078457/76‑1 (MQ=255)
cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa < 1:5776344/76‑1 (MQ=255)
cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa > 1:2541797/1‑76 (MQ=255)
gCCAGTTGAAGCGGATGCCTGTCTGATTGCTTTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 2:5246081/76‑1 (MQ=255)
gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:2315924/76‑1 (MQ=255)
gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc > 2:5443545/1‑76 (MQ=255)
gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:3024900/76‑1 (MQ=255)
ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg > 1:3128147/1‑76 (MQ=255)
aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt > 1:213747/1‑76 (MQ=255)
aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt < 2:4727195/76‑1 (MQ=255)
gTTGAAGCGGATGCCTGGCTGGTTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg < 2:5736131/76‑1 (MQ=255)
ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTCCGTTgc < 1:4092513/76‑2 (MQ=255)
ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa < 2:4402559/76‑1 (MQ=255)
tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 1:3809387/1‑76 (MQ=255)
tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 1:161422/1‑76 (MQ=255)
gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGCTGact > 2:583586/1‑74 (MQ=255)
aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 1:3221493/1‑76 (MQ=255)
aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc < 1:3378638/76‑1 (MQ=255)
aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 2:2122443/1‑76 (MQ=255)
aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGATCTAAGTTGAAtag > 1:1206142/1‑74 (MQ=255)
||
CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG > NC_000913/3969035‑3969171
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
