Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,969,103 |
2 bp→GC |
coding (232‑233/1263 nt) |
wecC → |
UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,969,103 | 0 | C | G | 100.0%
| 324.1
/ NA
| 121 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (67/54); total (67/54) |
| * | NC_000913 | 3,969,104 | 0 | G | C | 99.1%
| 288.4
/ ‑6.2
| 113 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (59/53); minor base A (1/0); total (60/53) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
Only 100 of 122 total aligned reads displayed.
GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG > NC_000913/3969034‑3969179
||
gCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc > 2:4117272/1‑76 (MQ=255)
ggAGTGTAGAAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct < 1:1374291/75‑1 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 2:3330756/1‑76 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:4041187/1‑76 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:1717027/1‑76 (MQ=255)
gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:2663188/1‑76 (MQ=255)
aGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa > 2:871010/1‑76 (MQ=255)
gtgtAGTAAAAACTGCCCTAGAAGGCGGCTTTTTACGACCGAGCCCCACGCCCGTTCCAGCGGATGCCTGGCTGAt > 2:4191409/1‑76 (MQ=255)
tgtAGTAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:4794521/76‑1 (MQ=255)
ggtagaaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:118836/75‑1 (MQ=255)
ggtagaaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 2:2781518/75‑1 (MQ=255)
tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc > 2:3428624/1‑76 (MQ=255)
tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:2271795/76‑1 (MQ=255)
tagtaAAAACTGCCGTAGAAGGCGGTGTTTTAAGAGCGAGCACGACGACAGTTGAAGCGGATGCCTGGCTGATTGc > 1:1824461/1‑76 (MQ=255)
agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 2:2892103/1‑76 (MQ=255)
agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 1:2964209/1‑76 (MQ=255)
gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg > 2:3769657/1‑76 (MQ=255)
aaaaacCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt < 1:1427065/75‑1 (MQ=255)
aaaacCTGCCCGAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa < 2:4722714/76‑1 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc < 1:1950392/76‑1 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 2:3413025/1‑76 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:305846/1‑76 (MQ=255)
aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:1523853/1‑76 (MQ=255)
accTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc < 2:3231444/74‑1 (MQ=255)
aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc > 2:2194196/1‑76 (MQ=255)
aCTGCCGTAGAAGGCGGTTTTTTACGAGCGCGCACGACGCCCGTTGACGCGGAAGCCTGGCTGATTGCTGTACCCa < 2:3902153/76‑1 (MQ=255)
cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc < 1:2048447/76‑1 (MQ=255)
cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc > 1:4614897/1‑76 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 2:3550335/1‑76 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:1153287/1‑76 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg < 1:3632134/76‑1 (MQ=255)
tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:1442411/1‑76 (MQ=255)
ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc < 1:780992/76‑1 (MQ=255)
cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 1:4371878/76‑1 (MQ=255)
cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 2:3646916/76‑1 (MQ=255)
cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg > 1:184288/1‑76 (MQ=255)
cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 2:3572358/76‑1 (MQ=255)
gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 2:2898197/1‑76 (MQ=255)
gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 1:1025357/1‑76 (MQ=255)
gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 1:1072648/1‑76 (MQ=255)
tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt > 2:2643802/1‑76 (MQ=255)
aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt > 1:3652187/1‑76 (MQ=255)
aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt < 2:454777/76‑1 (MQ=255)
gaGGCGGTGTTTTACGAGCGAGCGCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa < 2:3431232/75‑1 (MQ=255)
aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa > 1:1998876/1‑76 (MQ=255)
aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg < 1:4369826/76‑1 (MQ=255)
aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGCTTTAg > 2:1745993/1‑76 (MQ=255)
ggCGGTTTTTTATGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTTATTGCTGTACCCTCGCCGTTTAAgg < 2:3465652/76‑1 (MQ=255)
gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 1:2751846/76‑1 (MQ=255)
gCGGTTTTTGACGAGCGCGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 1:2900240/76‑1 (MQ=255)
cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc < 1:1032977/76‑1 (MQ=255)
cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc > 1:816785/1‑76 (MQ=255)
cGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTCTAACGGc > 2:280256/1‑76 (MQ=255)
ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:785236/1‑76 (MQ=255)
ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa < 2:3334029/76‑1 (MQ=255)
ttggagcggggAGGACGGCAGGTGGAGGGGATGCCTGGGTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 2:327599/73‑1 (MQ=255)
aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 1:4126167/76‑1 (MQ=255)
aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg > 2:3961549/1‑76 (MQ=255)
gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 1:4438203/76‑1 (MQ=255)
gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc > 1:4193391/1‑76 (MQ=255)
gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 2:816785/76‑1 (MQ=255)
gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc > 1:2352170/1‑76 (MQ=255)
gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGCTTAAGGGCGATCATGAGcc > 2:897350/1‑76 (MQ=255)
agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa < 1:4120590/76‑1 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 2:2409803/1‑76 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:4238743/1‑76 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 2:840154/1‑76 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 2:3593269/76‑1 (MQ=255)
gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:4640468/1‑76 (MQ=255)
gagcACGACGCCAGCTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGAGAGATCATGAGCCAGaa > 2:755584/1‑75 (MQ=255)
agcACGCCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAtata < 1:617633/76‑1 (MQ=255)
agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 1:78173/76‑1 (MQ=255)
agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 2:4087419/76‑1 (MQ=255)
agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata > 2:2384231/1‑76 (MQ=255)
agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata > 2:2615513/1‑76 (MQ=255)
cACGACGCGAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 2:3552266/76‑1 (MQ=255)
cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg > 2:3253029/1‑76 (MQ=255)
acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa < 2:1153286/76‑1 (MQ=255)
cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc < 2:3411941/76‑1 (MQ=255)
cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc < 2:3877891/76‑1 (MQ=255)
gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 1:3255053/1‑76 (MQ=255)
gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 2:4347594/1‑76 (MQ=255)
gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 2:3814893/1‑76 (MQ=255)
acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:2283747/76‑1 (MQ=255)
acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt > 1:2576051/1‑76 (MQ=255)
cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa < 1:3865297/76‑1 (MQ=255)
gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATGATGAGCCAGATATGACCTAc < 2:1705841/76‑1 (MQ=255)
gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:731339/76‑1 (MQ=255)
cctgTTGAAGAGGATGCCGGGCTGATTGCTGGACCCACGCCGCTTAAGGGCGATCATCAGCCAGATATCACCTACg > 2:1320615/4‑76 (MQ=255)
cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt < 1:3609951/76‑1 (MQ=255)
aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt > 2:3512407/1‑76 (MQ=255)
gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg > 2:3487649/1‑76 (MQ=255)
tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 2:3692873/1‑76 (MQ=255)
gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt < 2:4376377/76‑1 (MQ=255)
gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt < 2:4238743/76‑1 (MQ=255)
gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt > 1:4392666/1‑76 (MQ=255)
aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 1:3167812/1‑76 (MQ=255)
aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 2:1772286/1‑76 (MQ=255)
aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg < 1:1781850/76‑1 (MQ=255)
gctgggtgATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg < 1:328693/76‑1 (MQ=255)
||
GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG > NC_000913/3969034‑3969179
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
