Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,972,637 |
2 bp→GC |
coding (430‑431/546 nt) |
wecD → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,972,637 | 0 | C | G | 97.7%
| 324.0
/ NA
| 130 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base G (64/61); total (66/64) |
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 3,972,638 | 0 | G | C | 99.2%
| 338.1
/ NA
| 130 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (65/64); total (66/64) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 131 total aligned reads displayed.
GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC > NC_000913/3972567‑3972706
||
gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTcgc > 1:5117582/1‑76 (MQ=255)
gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCgg < 1:3130640/76‑1 (MQ=255)
aTTGGCGTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa < 2:256139/76‑1 (MQ=255)
ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa < 2:2977865/76‑1 (MQ=255)
ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCCTATGCTCGCGGTaa < 2:4736266/76‑1 (MQ=255)
tgggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa < 1:696792/74‑1 (MQ=255)
tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaa < 1:808381/76‑1 (MQ=255)
tGGCCTCCTGGCTGGACGAGGTGCAGGGGCTGAGCTGATGCAAACGGCGCTGAACTGCGTGTAGTGTCGAGGTata > 1:4454822/1‑74 (MQ=255)
ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaaa > 1:3040394/1‑76 (MQ=255)
gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaac < 2:3532822/76‑1 (MQ=255)
ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaaca > 1:4518316/1‑76 (MQ=255)
ctgctgGCTGGACGCGGTGCAGGTGCTGCGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaa < 2:4829553/76‑1 (MQ=255)
ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAcacaa > 1:5731044/1‑76 (MQ=255)
tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaac > 1:4567501/1‑76 (MQ=255)
gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACt < 2:2537708/76‑1 (MQ=255)
ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGGAAAACAACtt > 2:4912518/1‑76 (MQ=255)
tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt < 2:4742466/76‑1 (MQ=255)
tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt < 1:5362438/76‑1 (MQ=255)
gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGc < 1:4597212/76‑1 (MQ=255)
gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGc < 1:2389794/76‑1 (MQ=255)
ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCg < 1:883784/76‑1 (MQ=255)
gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAGCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 2:4873534/76‑1 (MQ=255)
gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 1:5469788/76‑1 (MQ=255)
gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt < 1:1143251/76‑1 (MQ=255)
gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt > 1:3216990/1‑76 (MQ=255)
gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:3767620/75‑1 (MQ=255)
gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:1211543/75‑1 (MQ=255)
aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg > 2:5525582/1‑76 (MQ=255)
aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg > 2:3486567/1‑76 (MQ=255)
aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg < 2:1381414/76‑1 (MQ=255)
cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg < 2:78926/76‑1 (MQ=255)
cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg < 2:2805616/76‑1 (MQ=255)
gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGc > 2:4259920/1‑76 (MQ=255)
gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGc > 1:1145746/1‑76 (MQ=255)
ggTGGAGGGGCGGAGCTGGTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa < 2:233962/76‑1 (MQ=255)
ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAACACAACTTTGCGGGGGGCgc > 2:5819699/1‑75 (MQ=255)
ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa > 2:2837023/1‑76 (MQ=255)
ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa > 1:3201533/1‑76 (MQ=255)
ggTGCAGGGGCGGAGCTGAGGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa < 2:5443874/76‑1 (MQ=255)
gTGCAGGTGCTGCGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAc < 1:5127167/76‑1 (MQ=255)
tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAcc > 2:1858779/1‑76 (MQ=255)
tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGCGGAGAcc > 1:2300007/1‑76 (MQ=255)
tGCAGGTGCGGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAca < 2:4889208/76‑2 (MQ=255)
gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGGGGCGAccc > 2:142773/1‑76 (MQ=255)
cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCa > 2:2385138/1‑76 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAGCTTTGCGGGTGGCGTCCCaa < 2:3420676/76‑1 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 2:2277872/1‑76 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 1:4730019/1‑76 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 2:4257131/1‑76 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaca > 1:2618272/1‑74 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 1:2792163/1‑76 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 2:4435956/1‑76 (MQ=255)
gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 1:5648692/76‑1 (MQ=255)
gctgagctgaTGCAAACGGCGCTAAACTGGGCGCATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAACgg > 1:4022595/1‑76 (MQ=255)
tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc < 1:1017148/76‑1 (MQ=255)
gagctgaTGCAAACGTCGCTAAACGAGTCTTATGCTCGCGGTAAACCAACGTTGCGGGTTGCGACCCAACTGTGCa < 1:2664159/76‑1 (MQ=255)
gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa < 2:927955/76‑1 (MQ=255)
ggctaaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa < 2:5731042/75‑1 (MQ=255)
gctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAac < 1:5002378/76‑1 (MQ=255)
ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca > 2:3874461/1‑76 (MQ=255)
ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca > 1:5736975/1‑76 (MQ=255)
gtGCAGACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg < 2:215507/75‑1 (MQ=255)
aTGCAAACGGCGCTAACCTGGGCTTATTCTCGCGTTAAAACAACTTTGCGGGGGTAGACCACAATGTGCACGAccg > 2:2727275/1‑76 (MQ=255)
aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 1:608573/1‑76 (MQ=255)
aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 2:4927557/1‑76 (MQ=255)
aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAACGGGCAACAccg > 2:2699638/1‑76 (MQ=255)
gCAACCGGCGCGAGACTGGGCGTATGCTCGCGGTAAAACCGCTTTGCGGGTGGCGGCCCAAATGGGCAACAgcgcc < 1:267100/76‑1 (MQ=255)
gCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:5525584/76‑1 (MQ=255)
gCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:2956394/76‑1 (MQ=255)
aaaaCGGCGCGAAGCGGGGCGTAGGCTCGCGGTAAAACACCTTGGGGGGTGGCGCCCAAAAGGGGCAACAccgccg < 2:5117581/75‑1 (MQ=255)
aaaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc > 1:3780863/1‑76 (MQ=255)
aggggCGCTAAGGGGGGCGTATGCTGGGGGTAAAACAACTTGGGGGGGGGCGACCCAAAGGGGCAACACCGCcgcg < 2:4287551/73‑1 (MQ=255)
accGGCGCAAGACTGGGCGGAGGCTGGCGGTAAAACAATTTGGCGGGTGGCGCCCAAAAGGGGCAACACCGCcgcg < 2:3123634/74‑1 (MQ=255)
aaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg > 2:3985863/1‑76 (MQ=255)
aCGGCGCTAAACTGGGCGTATGCTAGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc > 2:3326321/1‑76 (MQ=255)
cGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:823452/1‑76 (MQ=255)
cGGCGCTAAACGGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt < 1:1691591/76‑1 (MQ=255)
ggCGCTAACGTGGGCGTATGCTCGCGGTAAAACAACGTTGCGGTGGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:3626562/76‑1 (MQ=255)
ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:4883464/76‑1 (MQ=255)
ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt > 1:1734646/1‑76 (MQ=255)
ggCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt > 2:5304131/1‑76 (MQ=255)
gccgcAAAACGGGGCGTATCCCCGCGGAAAAACACCTTGGCGGGGGGCGACCCAAAGGGGCAACACCGCCGCGCtt < 1:1989849/74‑1 (MQ=255)
gcgcTAAACTGGGCGTATGCTCGCGGTACAACAACTTTGCGGGTGGATACCCAAATTTGCAACACCGCCGAGCCTa > 1:1485666/1‑76 (MQ=255)
gcgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa > 1:1441239/1‑76 (MQ=255)
gcgcGAAACGGGGGGTAGCCGCGCGGTAAAACAACTTTGCGGGTGGCGACCAAAAGGGGCAACACCGCCGCGCTTa < 2:1145746/76‑1 (MQ=255)
ggcTAAACGGGGCGTAGGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa < 2:3190285/75‑1 (MQ=255)
ggcTAAACGGGGCGTAGGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa < 2:3159511/75‑1 (MQ=255)
cgcTAACCGGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGCCCCAAATGGGCAACACCGCCGCGCTTaa < 1:3983023/76‑1 (MQ=255)
gcTAAACTGGGCGTATGCTTGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 1:4072583/1‑76 (MQ=255)
cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:1209590/1‑76 (MQ=255)
tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAcn < 1:3591325/76‑2 (MQ=255)
tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACg > 1:3062561/1‑76 (MQ=255)
tAAACGGGGCGTAGCCTCGGGGAAAACCAACTTGGGGGGTGGCGACCAAAGTGGGCAACCCCGCCGCGCTTAAACg < 2:1610880/76‑1 (MQ=255)
aaaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa > 1:1855574/1‑76 (MQ=255)
aCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata > 1:1750037/1‑76 (MQ=255)
cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac > 1:354706/1‑76 (MQ=255)
gggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 1:4078167/1‑76 (MQ=255)
gggCGTAGGGTGGCGGTAAAACAACTTTGCGGGGGGCGACCAAAAGGGGAAACACCGCCGCGCTTAAACGatacat < 2:5404472/76‑1 (MQ=255)
ggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata > 2:4839664/1‑76 (MQ=255)
gCGTATGCTCGCGGAAAAACAACTTGGCGGGTGGCGCCCAAAATGGGCAACACCGCCGCGCTTAAACGatacatac < 1:2450955/76‑1 (MQ=255)
||
GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC > NC_000913/3972567‑3972706
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
