Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,972,637 |
2 bp→GC |
coding (430‑431/546 nt) |
wecD → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,972,637 | 0 | C | G | 97.9%
| 243.1
/ ‑4.4
| 98 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (45/50); total (46/52) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
| * | NC_000913 | 3,972,638 | 0 | G | C | 99.0%
| 248.4
/ NA
| 99 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (45/52); minor base T (0/1); total (46/53) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCG‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT > NC_000913/3972569‑3972704
||
gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg > 1:3562088/1‑76 (MQ=255)
gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg > 1:2466837/1‑76 (MQ=255)
gAATGGGCGTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg < 2:4720266/76‑1 (MQ=255)
aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCggg > 2:1503881/1‑75 (MQ=255)
ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCATATGC‑TCGCGGTaa < 2:894182/76‑1 (MQ=255)
tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTaaa < 2:1976293/76‑1 (MQ=255)
ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTaaaa < 2:3119777/76‑1 (MQ=255)
gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTCTGC‑CCGCGGTcaaaa > 2:163098/1‑75 (MQ=255)
gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGGaaacc > 2:4716749/1‑74 (MQ=255)
ccggctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 2:567459/73‑1 (MQ=255)
ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca > 2:1629674/1‑76 (MQ=255)
ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 2:3591133/76‑1 (MQ=255)
ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 1:2538474/76‑1 (MQ=255)
tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaacaac > 1:129359/1‑76 (MQ=255)
gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACt < 1:2142482/76‑1 (MQ=255)
gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAAATGGGCGTATGC‑TCGCGGGAaaaaaaat > 1:2929825/1‑74 (MQ=255)
ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACtt < 2:2632803/76‑1 (MQ=255)
tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACttt > 2:4411707/1‑76 (MQ=255)
ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:1948162/76‑1 (MQ=255)
ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 1:2800779/76‑1 (MQ=255)
ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:2641966/76‑1 (MQ=255)
ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:347997/76‑1 (MQ=255)
ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:3506188/76‑1 (MQ=255)
gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGc < 2:677256/76‑1 (MQ=255)
gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGc < 1:2277429/76‑1 (MQ=255)
gtggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCg < 2:4084688/75‑1 (MQ=255)
tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCgg > 2:3342727/1‑76 (MQ=255)
tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCgg > 1:3863630/1‑76 (MQ=255)
tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑CCGCGGTAAAACAACTTTGccg > 2:207447/1‑74 (MQ=255)
ggACGCGGTGCGTGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCggg < 2:814451/76‑1 (MQ=255)
ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCggg > 1:2820920/1‑76 (MQ=255)
gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGTGGGTg < 2:4793592/75‑1 (MQ=255)
gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTg < 2:4120306/75‑1 (MQ=255)
gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc > 2:3291629/1‑76 (MQ=255)
gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc < 1:1640184/76‑1 (MQ=255)
gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc > 1:4829185/1‑76 (MQ=255)
cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCg < 1:4076754/76‑1 (MQ=255)
cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCg < 1:2353610/76‑1 (MQ=255)
cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGGGGCg > 2:1897794/1‑76 (MQ=255)
cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑GCGCGGTAAAACAACTTTGCGGGTGGCg < 1:4374971/76‑1 (MQ=255)
gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAc < 1:271802/76‑1 (MQ=255)
tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAcc > 2:2689546/1‑76 (MQ=255)
ggCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAcc < 2:4523851/75‑1 (MQ=255)
gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAccc > 1:986302/1‑76 (MQ=255)
cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCa > 1:2556182/1‑76 (MQ=255)
cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGCGGCCACCCa > 2:1160509/1‑76 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa < 1:4219208/76‑1 (MQ=255)
aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 1:1429084/1‑76 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACCACTTTGCGGGTGGCGCCCCaaa > 2:4820345/1‑76 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa < 1:959168/76‑1 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa < 1:2060543/76‑1 (MQ=255)
ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 1:4597180/1‑76 (MQ=255)
gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 1:164117/76‑1 (MQ=255)
gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 2:2359025/76‑1 (MQ=255)
gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg > 2:2002972/1‑76 (MQ=255)
gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg < 2:2679605/76‑1 (MQ=255)
gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg > 2:1773589/1‑76 (MQ=255)
ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg > 1:2748999/1‑76 (MQ=255)
ctgagctgaTGCAAACGGCGCTAAACTGGGCGTA‑CGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg > 1:4130212/1‑76 (MQ=255)
gagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa < 2:1826240/76‑1 (MQ=255)
gagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa > 1:3848755/1‑76 (MQ=255)
gggtgaTGCAAGCGGCGGTAGTCTGGGCGTATGT‑TCGCGGTAAAACAACTTTGCTGGTGGCGACCCAAATGGGCaa < 2:4723187/73‑1 (MQ=255)
ggctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa < 2:1180791/75‑1 (MQ=255)
agctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa > 1:1277817/1‑76 (MQ=255)
ctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca < 2:3397853/76‑1 (MQ=255)
ctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca < 2:3975595/76‑1 (MQ=255)
tgaTGCAAGCGGCGCTCAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGCCCCAAATGGGCAacac < 1:401259/76‑1 (MQ=255)
tgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac < 1:2858290/76‑1 (MQ=255)
gaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc > 2:2653047/1‑76 (MQ=255)
gaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAGGGGCAACAcc > 2:1642068/1‑76 (MQ=255)
aTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 2:3928940/1‑76 (MQ=255)
tGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGGGGCGCCCCAAATGGGCCACAcccc > 2:19140/1‑74 (MQ=255)
gCAATCGGCGCGAAACTGGGCGTAGTC‑TCGCGGTAAAGCAACTTTGCGGGTGGCGACCCAAATGGGCACCAccgcc < 1:3121189/76‑1 (MQ=255)
gCAAACGGCGCTAACCGGGGCGTATGC‑TCGCGGTAAAACACCTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:2429788/76‑1 (MQ=255)
gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:4105566/76‑1 (MQ=255)
gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:4278773/76‑1 (MQ=255)
gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:3763278/76‑1 (MQ=255)
gCAAACGGCGCTAAACGGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:436736/76‑1 (MQ=255)
cAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg > 1:2556576/1‑76 (MQ=255)
aagcGGCGCTAACCGGGGCGTATGC‑TCGCGGTAAAACATCTTTGGGGGTGGCGACCAAAAGGGGCAACACcgccgc < 1:4200378/73‑1 (MQ=255)
aaccGGCGCTAAACGGGGGGTATGC‑TCGCGTAAAACCAACTTGGCGGGTGGCGACCAAAAGGGCCACCCCcgccgc < 1:4106704/73‑1 (MQ=255)
aaaCGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc > 1:4147046/1‑76 (MQ=255)
aCGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc < 1:1962912/76‑1 (MQ=255)
cGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:3805876/1‑76 (MQ=255)
cGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:2883859/1‑76 (MQ=255)
cGGCGCGAAACTGGGCGTATGC‑TCGCGGTAAAACATCTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt < 2:4293248/76‑1 (MQ=255)
ggCGCTAAACGGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:2203413/76‑1 (MQ=255)
cgcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa > 1:185870/1‑76 (MQ=255)
gcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 2:3753872/1‑76 (MQ=255)
gcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 2:4387586/1‑76 (MQ=255)
cTAAGCGGGGCGTATGC‑GCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc < 2:1310995/76‑1 (MQ=255)
cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 1:2945781/1‑76 (MQ=255)
cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:3236861/1‑76 (MQ=255)
cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:3021310/1‑76 (MQ=255)
aCTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata > 1:751037/1‑76 (MQ=255)
aCTGGGCGTATCC‑TCGGGGAAAAACACCTGTGCGGGTGGCGCCCCAAAGGGGCAACACCGCCGCGCTTAAACGata < 1:1104903/76‑1 (MQ=255)
cggggCGTATCC‑CGGGGTTAAACCACCTTGGCGGGTGGGGCCCCAAGGGGGCAACCCCGCCGCGCTTAAACGatac < 2:3805019/74‑1 (MQ=255)
gggCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 1:2613564/1‑76 (MQ=255)
gggCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 2:4275506/1‑76 (MQ=255)
||
GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCG‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT > NC_000913/3972569‑3972704
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
