Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,544,384	C→T	pseudogene (381/381 nt)	yddK ←	pseudogene, leucine‑rich protein, putative glycoportein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,544,384	0	C	T	98.6%	231.2 / ‑4.3	72	pseudogene (381/381 nt)	yddK	pseudogene, leucine‑rich protein, putative glycoportein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (37/34); total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GCTAACCTTCAAACGGGGTTAATCTTTGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCACTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTCATGTTTTT  >  NC_000913/1544250‑1544509

gCTAACCTTCAAACGGGGTTAATCTTTGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTaaa                                                                                                                           >  2:73288/1‑139 (MQ=255)

      cTTCAAACGGGGTTAATCTTTGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGcc                                                                                                                     <  2:240685/139‑1 (MQ=255)

                ggTTAATCTTTGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTgga                                                                                                           <  1:300919/139‑1 (MQ=255)

                          tGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATGTTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTgga                                                                                                           >  2:540095/1‑129 (MQ=255)

                          tGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTgga                                                                                                           <  1:540095/129‑1 (MQ=255)

                          tGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 >  1:559843/1‑139 (MQ=255)

                          tGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 <  2:667903/139‑1 (MQ=255)

                          tGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 >  2:590862/1‑139 (MQ=255)

                           gAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATc                                                                                                >  1:551505/1‑139 (MQ=255)

                                 aTGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTcaa                                                                                          >  1:733151/1‑137 (MQ=255)

                                   gTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGa                                                                                        <  2:280946/139‑1 (MQ=255)

                                    tCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGaa                                                                                       <  1:700871/139‑1 (MQ=255)

                                           tCAACCCTTCAGATCGTTAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTAt                                                                                <  2:733151/139‑1 (MQ=255)

                                           tCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 >  2:530572/1‑122 (MQ=255)

                                           tCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 <  1:530572/122‑1 (MQ=255)

                                              aCCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCgg                                                                             >  2:108733/1‑139 (MQ=255)

                                                  ttCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTcc                                                                         >  2:784059/1‑139 (MQ=255)

                                                   tCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  1:648454/139‑1 (MQ=255)

                                                   tCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:247424/139‑1 (MQ=255)

                                                   tCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:316605/139‑1 (MQ=255)

                                                   tCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:442820/139‑1 (MQ=255)

                                                          gTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGACCAGAAGGTCTATCGGTTCCTCTCTTAt                                                                 >  2:480722/1‑139 (MQ=255)

                                                           tGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        >  1:46636/1‑131 (MQ=255)

                                                           tGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:46636/131‑1 (MQ=255)

                                                            gAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTg                                                               >  1:763968/1‑139 (MQ=255)

                                                              aTCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAg                                                                                         >  2:781368/1‑111 (MQ=255)

                                                              aTCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAg                                                                                         <  1:781368/111‑1 (MQ=255)

                                                                        ttACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGtaat                                                   >  1:715282/1‑139 (MQ=255)

                                                                         tACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:159513/117‑1 (MQ=255)

                                                                         tACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        >  1:159513/1‑117 (MQ=255)

                                                                           cATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTaataat                                                <  1:602648/139‑1 (MQ=255)

                                                                           cATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTaataat                                                <  2:421983/139‑1 (MQ=255)

                                                                           cATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCACTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTaataat                                                >  2:662487/1‑139 (MQ=255)

                                                                                tAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGtta                                           >  2:44207/1‑139 (MQ=255)

                                                                                 aaCTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGtaata                                                  >  1:750048/1‑131 (MQ=255)

                                                                                 aaCTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGtaata                                                  <  2:750048/131‑1 (MQ=255)

                                                                                  aCTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGttatt                                         <  2:551505/139‑1 (MQ=255)

                                                                                      tattatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACag                                     >  1:625081/1‑139 (MQ=255)

                                                                                      tattatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACag                                     >  1:167507/1‑139 (MQ=255)

                                                                                      tattatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACag                                     >  1:628340/1‑139 (MQ=255)

                                                                                       attatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagt                                    <  2:663766/139‑1 (MQ=255)

                                                                                        ttatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagta                                   >  1:97575/1‑139 (MQ=255)

                                                                                         tatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTaataat                                                >  2:62527/1‑125 (MQ=255)

                                                                                         tatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTaataat                                                <  1:62527/125‑1 (MQ=255)

                                                                                         tatCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagtaa                                  >  2:637872/1‑139 (MQ=255)

                                                                                            caacaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        <  2:241615/98‑1 (MQ=255)

                                                                                            caacaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCct                                                                        >  1:241615/1‑98 (MQ=255)

                                                                                            caacaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagtaagta                               <  2:167507/139‑1 (MQ=255)

                                                                                            caacaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagtaagta                               <  1:82739/139‑1 (MQ=255)

                                                                                              acaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTAtt                             >  1:718871/1‑139 (MQ=255)

                                                                                              acaaGACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTAtt                             >  2:704433/1‑139 (MQ=255)

                                                                                                  gACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTATTAATCGTTATTACAGTAAGTATTGttt                         >  1:210411/1‑139 (MQ=255)

                                                                                                  gACCAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGttt                         >  1:139839/1‑139 (MQ=255)

                                                                                                    ccAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTAt                       <  1:108733/139‑1 (MQ=255)

                                                                                                     cAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTAtt                      <  1:480722/139‑1 (MQ=255)

                                                                                                     cAGACACTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTAtt                      <  1:590862/139‑1 (MQ=255)

                                                                                                          acTTATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATc                 <  2:149450/139‑1 (MQ=255)

                                                                                                            ttATAGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTa               >  2:249492/1‑139 (MQ=255)

                                                                                                             tataGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 >  2:538724/1‑56 (MQ=255)

                                                                                                             tataGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 <  1:538724/56‑1 (MQ=255)

                                                                                                             tataGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTAt                       >  1:11704/1‑130 (MQ=255)

                                                                                                             tataGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTAt                       <  2:11704/130‑1 (MQ=255)

                                                                                                              ataGAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 >  2:106106/1‑55 (MQ=255)

                                                                                                              ataGAATTAGTGATGATTGATTCATTAAAAAGGCCATGAACTGGAGGAAAACGAt                                                                                                 <  1:106106/55‑1 (MQ=255)

                                                                                                                 gAATTAGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTc          <  2:625081/139‑1 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTTCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATGATCGTTATTACagtaagta                               <  2:532926/113‑1 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTTCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACagtaagta                               >  1:532926/1‑113 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCg                                              <  1:85359/98‑1 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCg                                              >  2:85359/1‑98 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTCATGtt     >  1:172455/1‑139 (MQ=255)

                                                                                                                      aGTGATGATTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTCATGtt     >  1:168282/1‑139 (MQ=255)

                                                                                                                         gatgatTTATTCATTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTCATGttttt  >  1:33844/1‑139 (MQ=255)

GCTAACCTTCAAACGGGGTTAATCTTTGAACGTATGTCATGTTTCAACCCTTCAGATCGTGAATCTAAAGGGTTACATATTAACTATATTATCAACAAGACCAGACACTTATAGAATTAGTGATGATTTATTCACTAAAAAGGCCATGAACTGGAGGAAAACGATCTGTCCAGAAGGTCTATCGGTTCCTCTCTTATTGCCTGATTGTAATAATCGTTATTACAGTAAGTATTGTTTATTTCATCTAAGGTCATGTTTTT  >  NC_000913/1544250‑1544509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A