Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,534,031 |
T→C |
intergenic (+10/‑35) |
ptsH → / → ptsI |
phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,534,031 | 0 | T | C | 100.0%
| 157.3
/ NA
| 44 | intergenic (+10/‑35) | ptsH/ptsI | phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/24); total (20/24) |
CCTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTG > NC_000913/2533901‑2534162
|
ccTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTaa > 2:137326/1‑139 (MQ=255)
tttAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAAt < 1:295689/139‑1 (MQ=255)
tAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCa < 2:115895/139‑1 (MQ=255)
aaCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGt < 2:52875/139‑1 (MQ=255)
aaCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGt < 2:197927/139‑1 (MQ=255)
aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc < 1:396777/139‑1 (MQ=255)
aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc < 2:30413/139‑1 (MQ=255)
cAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACaa < 1:528535/139‑1 (MQ=255)
aGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg < 1:137326/139‑1 (MQ=255)
gggCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAACAATCAGTCACAAGTAAGGTa > 2:729077/1‑139 (MQ=255)
gTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCa < 1:378238/139‑1 (MQ=255)
cGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCa > 1:337181/1‑139 (MQ=255)
tgACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTa > 1:505088/1‑139 (MQ=255)
gACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg < 2:472197/139‑1 (MQ=255)
aTCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATc > 2:312351/1‑139 (MQ=255)
tctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGc > 2:315851/1‑135 (MQ=255)
tctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGc < 1:315851/135‑1 (MQ=255)
ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATccc > 1:316689/1‑139 (MQ=255)
cGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg < 2:782237/115‑1 (MQ=255)
cGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg > 1:782237/1‑115 (MQ=255)
cGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTa > 1:572902/1‑139 (MQ=255)
cAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGc > 2:316157/1‑139 (MQ=255)
aGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGct < 1:13188/139‑1 (MQ=255)
aaGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTg < 1:591796/139‑1 (MQ=255)
aGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg > 2:142789/1‑106 (MQ=255)
aGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg < 1:142789/106‑1 (MQ=255)
cGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCtt < 2:359137/139‑1 (MQ=255)
ttGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTAt < 1:141544/84‑1 (MQ=255)
ttGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTAt > 2:141544/1‑84 (MQ=255)
ttGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTg > 2:180349/1‑139 (MQ=255)
tGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGAc < 1:180349/139‑1 (MQ=255)
ggTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACg > 2:88675/1‑139 (MQ=255)
ggTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACg > 1:2981/1‑139 (MQ=255)
gTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGa < 2:702412/139‑1 (MQ=255)
aCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGAc > 2:277869/1‑132 (MQ=255)
aCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGAc < 1:277869/132‑1 (MQ=255)
aTGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg > 1:200613/1‑50 (MQ=255)
aTGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg < 2:200613/50‑1 (MQ=255)
aTGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTa < 1:157726/63‑1 (MQ=255)
aTGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTa > 2:157726/1‑63 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc > 1:259350/1‑44 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc < 2:259350/44‑1 (MQ=255)
ggCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTg > 1:474981/1‑139 (MQ=255)
tCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTg < 1:229222/139‑1 (MQ=255)
|
CCTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTG > NC_000913/2533901‑2534162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
