Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,534,031 |
T→C |
intergenic (+10/‑35) |
ptsH → / → ptsI |
phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,534,031 | 0 | T | C | 100.0%
| 161.4
/ NA
| 47 | intergenic (+10/‑35) | ptsH/ptsI | phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/23); total (24/23) |
TTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA > NC_000913/2533905‑2534166
|
tttAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAAt < 2:374535/139‑1 (MQ=255)
tAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCa < 2:21607/139‑1 (MQ=255)
tGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTcac < 1:771080/139‑1 (MQ=255)
aGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg < 1:949388/139‑1 (MQ=255)
tGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGt < 2:548656/139‑1 (MQ=255)
tGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGAAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGtt > 2:247642/1‑139 (MQ=255)
aCTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc > 2:587481/1‑120 (MQ=255)
aCTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc < 1:587481/120‑1 (MQ=255)
aaGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGAtt > 2:33021/1‑139 (MQ=255)
gTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCa < 1:68376/139‑1 (MQ=255)
ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGc > 2:460625/1‑139 (MQ=255)
cGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTaaaaa > 1:229252/1‑103 (MQ=255)
cGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTaaaaa < 2:229252/103‑1 (MQ=255)
cGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCa > 2:922185/1‑139 (MQ=255)
gTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCAt > 1:735239/1‑139 (MQ=255)
gtgACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCAtttt > 2:317697/1‑139 (MQ=255)
gACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg < 1:33021/139‑1 (MQ=255)
aCTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGc > 2:829769/1‑139 (MQ=255)
ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAgg < 2:463576/107‑1 (MQ=255)
ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAgg > 1:463576/1‑107 (MQ=255)
tcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc < 2:375638/96‑1 (MQ=255)
tcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc > 1:375638/1‑96 (MQ=255)
cAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGt < 1:829769/139‑1 (MQ=255)
aGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTa < 1:250112/139‑1 (MQ=255)
cAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGc > 2:334575/1‑139 (MQ=255)
aGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGc > 2:308165/1‑139 (MQ=255)
ttGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTg > 1:952649/1‑139 (MQ=255)
tAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAgg < 1:120555/80‑1 (MQ=255)
tAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGATTATGATTTCAgg > 2:120555/1‑80 (MQ=255)
aaaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCa < 2:560827/89‑1 (MQ=255)
aaaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTGTAGCa > 1:560827/1‑89 (MQ=255)
aCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGctct > 1:479733/1‑116 (MQ=255)
aCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGctct < 2:479733/116‑1 (MQ=255)
gATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCAGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCa > 1:468329/1‑139 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTGTGATTTCAGGCATTTTAg > 2:40739/1‑80 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTGTGATTTCAGGCATTTTAg < 1:40739/80‑1 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATcc > 2:389429/1‑85 (MQ=255)
tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATcc < 1:389429/85‑1 (MQ=255)
gAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAaaga > 2:1047472/1‑118 (MQ=255)
gAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAaaga < 1:1047472/118‑1 (MQ=255)
gAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGaaaaa > 1:945773/1‑139 (MQ=255)
aTTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg < 2:36826/64‑1 (MQ=255)
aTTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg > 1:36826/1‑64 (MQ=255)
aTTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAAttt > 1:360269/1‑139 (MQ=255)
ttCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCAGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCt < 2:468329/139‑1 (MQ=255)
tCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTg < 2:618164/139‑1 (MQ=255)
gggCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGa < 2:945773/139‑1 (MQ=255)
|
TTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGA > NC_000913/2533905‑2534166
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
