Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,295	C→T	R21H (CGT→CAT)	der ←	GTPase, multicopy suppressor of ftsJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,295	0	C	T	100.0%	113.7 / NA	36	R21H (CGT→CAT)	der	GTPase, multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637163‑2637426

tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               <  1:54600/139‑1 (MQ=255)

  ttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAAt                                                                                                                             >  2:701258/1‑139 (MQ=255)

          cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGa                                                                                                                     <  1:628140/139‑1 (MQ=255)

           aTCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  1:149298/139‑1 (MQ=255)

           aTCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  1:336713/139‑1 (MQ=255)

                                   gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                            >  2:20656/1‑139 (MQ=255)

                                    aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACaa                                                                                           >  2:755820/1‑139 (MQ=255)

                                         cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                                      >  1:434155/1‑139 (MQ=255)

                                           cACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                                                                    <  2:619027/139‑1 (MQ=255)

                                           cACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAc                                                                                    <  1:182892/139‑1 (MQ=255)

                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTTGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAcc                                                                                   >  2:697050/1‑139 (MQ=255)

                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAcc                                                                                   >  1:764591/1‑139 (MQ=255)

                                                   tCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTa                                                                            >  1:787140/1‑139 (MQ=255)

                                                     aaTTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                                          <  1:301966/139‑1 (MQ=255)

                                                     aaTTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                                          <  1:755820/139‑1 (MQ=255)

                                                              aCGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTaaa                                                                 >  2:403388/1‑139 (MQ=255)

                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  1:115917/1‑139 (MQ=255)

                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGA‑TTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAACACCTCATTTTtatac                                                  >  1:772975/1‑138 (MQ=255)

                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  2:537980/1‑59 (MQ=255)

                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               <  1:537980/59‑1 (MQ=255)

                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                                               >  1:198194/1‑139 (MQ=255)

                                                                                           ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  2:434155/139‑1 (MQ=255)

                                                                                           ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  2:772977/139‑1 (MQ=255)

                                                                                           ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  1:790552/139‑1 (MQ=255)

                                                                                           ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATGTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  1:484845/139‑1 (MQ=255)

                                                                                             gggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgca                                  <  1:142463/139‑1 (MQ=255)

                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAggg                                                                                                     >  1:595581/1‑71 (MQ=255)

                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAggg                                                                                                     <  2:595581/71‑1 (MQ=255)

                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAt                                                          <  2:205417/114‑1 (MQ=255)

                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAt                                                          >  1:205417/1‑114 (MQ=255)

                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcac                                 >  1:750038/1‑139 (MQ=255)

                                                                                                        aaCCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTcg                       <  2:750038/139‑1 (MQ=255)

                                                                                                                    tcGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTaa           <  2:211031/139‑1 (MQ=255)

                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  2:55593/105‑1 (MQ=255)

                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    >  1:55593/1‑105 (MQ=255)

                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGaa  >  1:533674/1‑139 (MQ=255)

TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637163‑2637426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A