Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,295	C→T	R21H (CGT→CAT)	der ←	GTPase, multicopy suppressor of ftsJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,295	0	C	T	100.0%	123.7 / NA	39	R21H (CGT→CAT)	der	GTPase, multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/18); total (21/18)

CGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637161‑2637426

cGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGtt                                                                                                                                 >  2:322383/1‑139 (MQ=255)

  tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  2:957338/1‑139 (MQ=255)

  tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  1:829670/1‑139 (MQ=255)

    ttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAAt                                                                                                                             >  2:213753/1‑139 (MQ=255)

       tgtgCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAAc                                                                                                                          >  1:568403/1‑139 (MQ=255)

            cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGa                                                                                                                     >  1:335435/1‑139 (MQ=255)

            cATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGa                                                                                                                     <  2:890474/139‑1 (MQ=255)

             aTCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  1:957338/139‑1 (MQ=255)

                aaTCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAtttt                                                                                                                 <  1:855347/139‑1 (MQ=255)

                         ggTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTa                                                                                                        <  2:829670/139‑1 (MQ=255)

                               aaTACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  1:213980/125‑1 (MQ=255)

                               aaTACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  2:213980/1‑125 (MQ=255)

                                   cAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGa                                                                                              <  2:335435/139‑1 (MQ=255)

                                     gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  1:684748/119‑1 (MQ=255)

                                     gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  2:684748/1‑119 (MQ=255)

                                     gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                            >  2:88134/1‑139 (MQ=255)

                                           cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  1:813892/109‑1 (MQ=255)

                                           cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    >  2:813892/1‑109 (MQ=255)

                                           cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  2:465/113‑1 (MQ=255)

                                           cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  1:465/1‑113 (MQ=255)

                                              aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCAGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGAcc                                                                                   <  1:213753/139‑1 (MQ=255)

                                                                       tACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  1:453957/85‑1 (MQ=255)

                                                                       tACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  2:453957/1‑85 (MQ=255)

                                                                          ttACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCAtttt                                                       <  1:322383/139‑1 (MQ=255)

                                                                             cGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                           <  1:901891/132‑1 (MQ=255)

                                                                             cGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                           <  1:892933/132‑1 (MQ=255)

                                                                             cGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                           >  2:892933/1‑132 (MQ=255)

                                                                             cGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                           >  2:901891/1‑132 (MQ=255)

                                                                              ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                                      >  2:793790/1‑104 (MQ=255)

                                                                              ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                                      <  1:793790/104‑1 (MQ=255)

                                                                              ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  2:641275/1‑139 (MQ=255)

                                                                              ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  2:687733/1‑139 (MQ=255)

                                                                              ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   <  1:88134/139‑1 (MQ=255)

                                                                                                ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGTGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcac                                 >  2:826253/1‑139 (MQ=255)

                                                                                                                cgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcacg                                >  1:233372/1‑124 (MQ=255)

                                                                                                                cgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcacg                                <  2:233372/124‑1 (MQ=255)

                                                                                                                          gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    >  1:859954/1‑110 (MQ=255)

                                                                                                                          gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  2:859954/110‑1 (MQ=255)

                                                                                                                               aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGaa  >  1:699830/1‑139 (MQ=255)

CGTCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637161‑2637426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A