Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,637,295 C→T R21H (CGT→CAT)  der ← GTPase, multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,2950CT100.0% 178.9 / NA 55R21H (CGT→CAT) derGTPase, multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637163‑2637426
                                                                                                                                    |                                                                                                                                   
tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  1:892408/1‑139 (MQ=255)
tcttctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  1:478401/1‑139 (MQ=255)
tcttctGTGCCATCAATCCCGCCGGTATCAATACAAATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  2:1408162/1‑139 (MQ=255)
         ccATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTgg                                                                                                                      <  1:287265/139‑1 (MQ=255)
               aTCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  2:387865/139‑1 (MQ=255)
                                   gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                            >  2:1508006/1‑139 (MQ=255)
                                    aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  1:1501029/114‑1 (MQ=255)
                                    aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    >  2:1501029/1‑114 (MQ=255)
                                    aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACaa                                                                                           >  1:1600482/1‑139 (MQ=255)
                                       aaCTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgcg                                                                                        >  2:348744/1‑139 (MQ=255)
                                         cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                                      <  1:1508006/139‑1 (MQ=255)
                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               <  1:1374375/95‑1 (MQ=255)
                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTaa                                                                                                                               >  2:1374375/1‑95 (MQ=255)
                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  1:847827/1‑110 (MQ=255)
                                            aCGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  2:847827/110‑1 (MQ=255)
                                                ccTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAg                                                                               <  2:1578517/139‑1 (MQ=255)
                                                          ccGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  2:1006008/96‑1 (MQ=255)
                                                          ccGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  1:1006008/1‑96 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAGTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  1:624130/1‑139 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    <  2:1284669/74‑1 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                                    >  1:1284669/1‑74 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                <  2:376903/78‑1 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTc                                                                                                                >  1:376903/1‑78 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  1:657540/1‑139 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   <  2:892408/139‑1 (MQ=255)
                                                                            ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                                   >  2:427115/1‑139 (MQ=255)
                                                                             gTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATaa                                                  >  1:624004/1‑139 (MQ=255)
                                                                               cACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgcg                                                                                        <  1:1290642/99‑1 (MQ=255)
                                                                               cACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgcg                                                                                        >  2:1290642/1‑99 (MQ=255)
                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgc                                                                                         <  2:409481/97‑1 (MQ=255)
                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgc                                                                                         >  1:409481/1‑97 (MQ=255)
                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                                               >  1:821123/1‑139 (MQ=255)
                                                                                aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                                               >  2:1542446/1‑139 (MQ=255)
                                                                                 cGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatca                                              <  1:565750/139‑1 (MQ=255)
                                                                                  gAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatcat                                             <  1:427115/139‑1 (MQ=255)
                                                                                  gAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatcat                                             <  2:821123/139‑1 (MQ=255)
                                                                                      cAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGa                                         <  2:478401/139‑1 (MQ=255)
                                                                                      cAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGa                                         <  1:1408162/139‑1 (MQ=255)
                                                                                       aGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAc                                                                           >  2:1391556/1‑104 (MQ=255)
                                                                                       aGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAc                                                                           <  1:1391556/104‑1 (MQ=255)
                                                                                           ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  1:348744/139‑1 (MQ=255)
                                                                                            cGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAGTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgc                                   <  2:624130/139‑1 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGt                                                                                                                        >  2:97073/1‑52 (MQ=255)
                                                                                              ggAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGt                                                                                                                        <  1:97073/52‑1 (MQ=255)
                                                                                               gAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcacg                                >  2:1490304/1‑139 (MQ=255)
                                                                                                   tCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    <  2:372766/131‑1 (MQ=255)
                                                                                                   tCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                                    >  1:372766/1‑131 (MQ=255)
                                                                                                     cGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                            >  2:1057133/1‑73 (MQ=255)
                                                                                                     cGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                            <  1:1057133/73‑1 (MQ=255)
                                                                                                                   ctcGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGcgtcg                  <  2:376566/133‑1 (MQ=255)
                                                                                                                   ctcGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGcgtcg                  >  1:376566/1‑133 (MQ=255)
                                                                                                                        gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                                          <  2:735642/72‑1 (MQ=255)
                                                                                                                        gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                                          >  1:735642/1‑72 (MQ=255)
                                                                                                                           cGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAgg    <  2:926578/139‑1 (MQ=255)
                                                                                                                             aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGaa  >  2:1028237/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TCTTCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAA  >  NC_000913/2637163‑2637426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A